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The map label for this gene is nylB [H]

Identifier: 159184534

GI number: 159184534

Start: 917134

End: 918441

Strand: Reverse

Name: nylB [H]

Synonym: Atu0929

Alternate gene names: 159184534

Gene position: 918441-917134 (Counterclockwise)

Preceding gene: 15888269

Following gene: 15888266

Centisome position: 32.32

GC content: 61.7

Gene sequence:

>1308_bases
ATGCTTGCGGCGGTTCTGCCGGTTCTGGTGTTCCCGCACGGCGCTTCCAGTCAGGTTGCCACCCCGCTTTCGGCGGAGCA
GTCGGACCCGCTGAAGCTCGGCTGGATGCAGGGCTTTCCGCCACCGGAAGACAAGATCATCCGCTTTTCCGACAGTGACT
ATTTCAACTTTCCGAAATCGCGCTGGACGGTCTGCCACTTTCGCCAGCTGATGCCGACCGTCAATGTAAGCCGTGGTCTC
GGCGCACCGGCAGCGCTTGAGCGCAAGCTCGATCCCGCCATGGACGCCGTGACCTTCCGGCCGACAGGCGCCGATCAAGA
CATGACGTGGCTGCAATCGCTATCGGCGAATTATACCGACGGCATCGTCGTGCTGCACAAGGGTGTGCTCGTCTATGAGC
GTTATTCCGGCTGCCTGAACGAGGCGGGGCAACATGGCGCCATGTCGGTGACGAAGTCGCTGACCGGCCTTCTCGGTGAA
ATGCTGGTTGCCGAAGGCACGCTGGACGAGAATGCGAAGGTCGCGACCGTCGTTCCCGAACTTGAAAAAAGCGCCTTTGG
CGATGCGACGATCCGGCAGGTTCTCGATATGACGACCGGCCTGCGTTACAGCGAAGACTATGCCGACCCCAATGCCGATG
TGTGGATCTATTCGGCCGCCGGCAATCCGCTTCCCAAGCCCGATGGTTACGAAGGCCCACGCAGCTATTTCGATTATCTG
AAAACGGTGGTGAAACAGGGCGAAAACGGGTCTGCCTTCGGCTACAAGACCATCAACAGCGATGCTCTGGGCTGGGTCAT
TGCCCGCGCTTCGGGAAAATCGGTGGCGGAGCTGCTGTCGCAGCGGATCTGGAGCCGGATAGGCGCCGAGCAGGACGCTT
ATTATACGGTTGATTCCACCGGCACACCCTTTGCCGGCGGCGGCCTGAATGCCGGGTTGCGTGACATGGCGCGCATTGGC
CAGTTGCTGCTTGATGACGGCATGGCCGGCGGCCATCGTCTCATTCCGCAGAAGGCGATCGACAATATCCGCCACGGCGG
CGATAAGGCGGCTTTTGCGAAGGGCGGTTATCCGGCATTGCGGGGCTGGAGCTATCGCGCCATGTGGTGGGTCACCCACA
ATGCCAATGGCGCCTTCATGGCGCGGGGCGTGCACGGTCAGGCAATCTATGTCGACCCGGCCGCCGACATGGTCATCGCC
CGCTTTTCCTCGCACCCCGTCGCCAGCAATGCCGCCAACGACCCGACATCGCTGCCCGCCTATGAGGCCGTGGCGCGTTA
TTTGATGGGAGGTGCGGCTAGGCCGTGA

Upstream 100 bases:

>100_bases
TTTCCCGGCAAGGGCATGGAATCCATGACTGCAGAAAATTCGCCGACCTGGCTTGGAGGTTTCGTCAATGTCATCGTCAA
TTTCTAGCGCCCTGTCGATC

Downstream 100 bases:

>100_bases
GTCTGTGCTTGGCTGGGATAGTGTGCGTGGTCCGGACCATCGCTTGACCGGGACGGCCCGGAAGGTGGCGATGCGGCTCG
GCGGTGATATCCGGGCGCAT

Product: amide hydrolase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MLAAVLPVLVFPHGASSQVATPLSAEQSDPLKLGWMQGFPPPEDKIIRFSDSDYFNFPKSRWTVCHFRQLMPTVNVSRGL
GAPAALERKLDPAMDAVTFRPTGADQDMTWLQSLSANYTDGIVVLHKGVLVYERYSGCLNEAGQHGAMSVTKSLTGLLGE
MLVAEGTLDENAKVATVVPELEKSAFGDATIRQVLDMTTGLRYSEDYADPNADVWIYSAAGNPLPKPDGYEGPRSYFDYL
KTVVKQGENGSAFGYKTINSDALGWVIARASGKSVAELLSQRIWSRIGAEQDAYYTVDSTGTPFAGGGLNAGLRDMARIG
QLLLDDGMAGGHRLIPQKAIDNIRHGGDKAAFAKGGYPALRGWSYRAMWWVTHNANGAFMARGVHGQAIYVDPAADMVIA
RFSSHPVASNAANDPTSLPAYEAVARYLMGGAARP

Sequences:

>Translated_435_residues
MLAAVLPVLVFPHGASSQVATPLSAEQSDPLKLGWMQGFPPPEDKIIRFSDSDYFNFPKSRWTVCHFRQLMPTVNVSRGL
GAPAALERKLDPAMDAVTFRPTGADQDMTWLQSLSANYTDGIVVLHKGVLVYERYSGCLNEAGQHGAMSVTKSLTGLLGE
MLVAEGTLDENAKVATVVPELEKSAFGDATIRQVLDMTTGLRYSEDYADPNADVWIYSAAGNPLPKPDGYEGPRSYFDYL
KTVVKQGENGSAFGYKTINSDALGWVIARASGKSVAELLSQRIWSRIGAEQDAYYTVDSTGTPFAGGGLNAGLRDMARIG
QLLLDDGMAGGHRLIPQKAIDNIRHGGDKAAFAKGGYPALRGWSYRAMWWVTHNANGAFMARGVHGQAIYVDPAADMVIA
RFSSHPVASNAANDPTSLPAYEAVARYLMGGAARP
>Mature_435_residues
MLAAVLPVLVFPHGASSQVATPLSAEQSDPLKLGWMQGFPPPEDKIIRFSDSDYFNFPKSRWTVCHFRQLMPTVNVSRGL
GAPAALERKLDPAMDAVTFRPTGADQDMTWLQSLSANYTDGIVVLHKGVLVYERYSGCLNEAGQHGAMSVTKSLTGLLGE
MLVAEGTLDENAKVATVVPELEKSAFGDATIRQVLDMTTGLRYSEDYADPNADVWIYSAAGNPLPKPDGYEGPRSYFDYL
KTVVKQGENGSAFGYKTINSDALGWVIARASGKSVAELLSQRIWSRIGAEQDAYYTVDSTGTPFAGGGLNAGLRDMARIG
QLLLDDGMAGGHRLIPQKAIDNIRHGGDKAAFAKGGYPALRGWSYRAMWWVTHNANGAFMARGVHGQAIYVDPAADMVIA
RFSSHPVASNAANDPTSLPAYEAVARYLMGGAARP

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 46857; Mature: 46857

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAAVLPVLVFPHGASSQVATPLSAEQSDPLKLGWMQGFPPPEDKIIRFSDSDYFNFPKS
CHHHHEEHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCCCCC
RWTVCHFRQLMPTVNVSRGLGAPAALERKLDPAMDAVTFRPTGADQDMTWLQSLSANYTD
HHHHHHHHHHCCCCCHHCCCCCCHHHHHHCCHHHHCEEECCCCCCCHHHHHHHHCCCCCC
GIVVLHKGVLVYERYSGCLNEAGQHGAMSVTKSLTGLLGEMLVAEGTLDENAKVATVVPE
CEEEEECCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCH
LEKSAFGDATIRQVLDMTTGLRYSEDYADPNADVWIYSAAGNPLPKPDGYEGPRSYFDYL
HHHHHCCHHHHHHHHHHHHCCCCCHHCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
KTVVKQGENGSAFGYKTINSDALGWVIARASGKSVAELLSQRIWSRIGAEQDAYYTVDST
HHHHHCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCC
GTPFAGGGLNAGLRDMARIGQLLLDDGMAGGHRLIPQKAIDNIRHGGDKAAFAKGGYPAL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCHHH
RGWSYRAMWWVTHNANGAFMARGVHGQAIYVDPAADMVIARFSSHPVASNAANDPTSLPA
CCCCEEEEEEEEECCCCEEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCH
YEAVARYLMGGAARP
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MLAAVLPVLVFPHGASSQVATPLSAEQSDPLKLGWMQGFPPPEDKIIRFSDSDYFNFPKS
CHHHHEEHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCCCCC
RWTVCHFRQLMPTVNVSRGLGAPAALERKLDPAMDAVTFRPTGADQDMTWLQSLSANYTD
HHHHHHHHHHCCCCCHHCCCCCCHHHHHHCCHHHHCEEECCCCCCCHHHHHHHHCCCCCC
GIVVLHKGVLVYERYSGCLNEAGQHGAMSVTKSLTGLLGEMLVAEGTLDENAKVATVVPE
CEEEEECCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCH
LEKSAFGDATIRQVLDMTTGLRYSEDYADPNADVWIYSAAGNPLPKPDGYEGPRSYFDYL
HHHHHCCHHHHHHHHHHHHCCCCCHHCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
KTVVKQGENGSAFGYKTINSDALGWVIARASGKSVAELLSQRIWSRIGAEQDAYYTVDST
HHHHHCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCC
GTPFAGGGLNAGLRDMARIGQLLLDDGMAGGHRLIPQKAIDNIRHGGDKAAFAKGGYPAL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCHHH
RGWSYRAMWWVTHNANGAFMARGVHGQAIYVDPAADMVIARFSSHPVASNAANDPTSLPA
CCCCEEEEEEEEECCCCEEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCH
YEAVARYLMGGAARP
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 2512123 [H]