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The map label for this gene is sigK [H]

Identifier: 159184326

GI number: 159184326

Start: 462837

End: 463382

Strand: Direct

Name: sigK [H]

Synonym: Atu0474

Alternate gene names: 159184326

Gene position: 462837-463382 (Clockwise)

Preceding gene: 159184324

Following gene: 159184327

Centisome position: 16.29

GC content: 61.17

Gene sequence:

>546_bases
ATGGGCTCCGCGCCTGATGATCTCGACAGGGCGCTCGCCGCCTGCGCGAGAGGCGACCGAAACGCATTGCGGGTTATTTT
CGAGCGCGAGGCAGGGCGACTGATCGCGGTGGCCGAGCGGATCGTCAAACGCAGGGAACTGGCAGAAGAGGTTGTGCAGG
AGAGTTTCGTGCGCATCTGGACGCACGCGCCCCAATATCGAGCCGACCACGGTTCGGCGCGTGGCTGGATTTACGCCATT
GTCAGGAACCGGGCGCTTAATCTTCTGCGGGACGGGAGCCGCGAACTTACCGTGCAGGATGTCGAAACGCTGCGTGACAG
CGAACAGGCGGATGAAGTGGTCGCCGCCTGGCAGAAGCTCGACAGGAATTCACGTCTTTATGAATGTCTGGGCGCGCTCG
ACGAGACCAAACGGCAGGGTATTTTGATGGCTTATGTCGCCGGATATTCCCATGGCGAAATCGCCGGCCGGTTGCGCGTT
CCGCTTGGCACGACGAAATCCTGGATAAGGCGCGGGCTTTCCGCTCTTCGGGAGTGCATGGCATGA

Upstream 100 bases:

>100_bases
GCAAAAAAATATCTGCTTATGCATCCATTTCGTTCCGCGATGGGTATTATACCAATTCCGACCGTGCCTGCGGTCCAAAC
TTGAAAGGTTTCGTATGCGT

Downstream 100 bases:

>100_bases
CATATGATCAAAAAGACATTCCCGCCATTGCCGATGACTATGTATTGGGGCTGCTCGAGGCGTCCGAGGCTGCTGCTATC
GAAACGGCAACGGAGCGGGA

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-K factor [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MGSAPDDLDRALAACARGDRNALRVIFEREAGRLIAVAERIVKRRELAEEVVQESFVRIWTHAPQYRADHGSARGWIYAI
VRNRALNLLRDGSRELTVQDVETLRDSEQADEVVAAWQKLDRNSRLYECLGALDETKRQGILMAYVAGYSHGEIAGRLRV
PLGTTKSWIRRGLSALRECMA

Sequences:

>Translated_181_residues
MGSAPDDLDRALAACARGDRNALRVIFEREAGRLIAVAERIVKRRELAEEVVQESFVRIWTHAPQYRADHGSARGWIYAI
VRNRALNLLRDGSRELTVQDVETLRDSEQADEVVAAWQKLDRNSRLYECLGALDETKRQGILMAYVAGYSHGEIAGRLRV
PLGTTKSWIRRGLSALRECMA
>Mature_180_residues
GSAPDDLDRALAACARGDRNALRVIFEREAGRLIAVAERIVKRRELAEEVVQESFVRIWTHAPQYRADHGSARGWIYAIV
RNRALNLLRDGSRELTVQDVETLRDSEQADEVVAAWQKLDRNSRLYECLGALDETKRQGILMAYVAGYSHGEIAGRLRVP
LGTTKSWIRRGLSALRECMA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 20420; Mature: 20288

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSAPDDLDRALAACARGDRNALRVIFEREAGRLIAVAERIVKRRELAEEVVQESFVRIW
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
THAPQYRADHGSARGWIYAIVRNRALNLLRDGSRELTVQDVETLRDSEQADEVVAAWQKL
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHH
DRNSRLYECLGALDETKRQGILMAYVAGYSHGEIAGRLRVPLGTTKSWIRRGLSALRECM
CCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure 
GSAPDDLDRALAACARGDRNALRVIFEREAGRLIAVAERIVKRRELAEEVVQESFVRIW
CCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
THAPQYRADHGSARGWIYAIVRNRALNLLRDGSRELTVQDVETLRDSEQADEVVAAWQKL
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHH
DRNSRLYECLGALDETKRQGILMAYVAGYSHGEIAGRLRVPLGTTKSWIRRGLSALRECM
CCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA