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The map label for this gene is virB4 [H]

Identifier: 159046515

GI number: 159046515

Start: 34700

End: 37063

Strand: Direct

Name: virB4 [H]

Synonym: Dshi_3976

Alternate gene names: 159046515

Gene position: 34700-37063 (Clockwise)

Preceding gene: 159046514

Following gene: 159046516

Centisome position: 27.47

GC content: 62.86

Gene sequence:

>2364_bases
ATGCCCCGTGAAGCCGCGCTTGATCTCCGCACGATGACGCCGGAGTGGTATGCGCGCGAGACCCGCCTCGCGCACATGCT
GCCCTATGTGAGCCTCGTCGATCACCAGACCGTGCGGACCCGGGTCAACGAGCTTTTCCAGTGCATCCGGCTCGACGGGG
TCAATAGCTATACGACCGATGACGCCTATCTCGACAAGGTGACCGCGCTCTTTGCCCGGATCATTGCGCAATTGGGGCCG
GAGTTCAGCTATTACGTCCATAAGGTCTCGAAGGCGATCACGCCGGACCTCGAGCCGCTGCGCGAGGACAGTTTCGCGGG
AGAAGTCGACCGGCTCTGGCGCAAGAAGCTAGAGACCAGCGGGCTGCGCGACAAGACGCTCACCCTTACGATCATCCATC
GCCCGCCTCCAAAAAGCGTCCTGCCATTCCTGAACCGCAGCGCGCCGGATCGCCTCAAGGAAGCGACCGAGAAGCGCCTT
CGCCGATTGGGCGAGGCCGTGAACGTGTTCCTGTCTGGCCTGACGGAGCTTAACCCACGTGTGCTTTCGGCCAAGTCCGG
CGAGTTGATTGGCTTCCTAGGGGCCCTCAACACTGGCCAGGAACTGCCGCTCTATCCGGCGAACACCTATGGCTTCCTGT
CCTTCAACGTCGCCAACACGCGAGTGACGTTTCAAGGCGATCATTTCGAGCTCTCGGAAGGCGTCGTGGGCCATCGCTAC
GGCAAGAGTTTCACCATCGGGGAATACTCGGAAGGCACCTCCTGCACCATGTTCGACATGCTGAACCTGCCGGTCGACAT
GATCGTCACCCATTCCTTCACACCGATCAATTCGAACCTCATGGCGGGCCGCATCAAGCGGCAAAAGCGCCAGATGCAGG
CGAGCCAGGACGCGGCCCTCTCGCTCCTGGAAGCGCTCGACATCGCCGCCGATGATCTCGAGGCCAAGCGCCAAAGCTTC
GGCGAGCATCACATGGTCGTGACGCTCTTCTGCGAAACGCTGGACGAGCTGCAGACGCTCAGCGCCGAAATCGTGAACGC
CGCCGCGACCGAAGGCGTGAAGATGATCGGTGAGCGGGTCGCCGCAAAGGCCCATTACCTCAGCCAGCATCCCGGCAACC
AGCCCAAGCGCGTGCGTGCCAGCGCCGTCACCAATCGCAACTTCGCGGATTTTGCGGCCTTTCACCGGACACAGCTCGGC
AAACCCGCAGCACTTACCCCTTGGGGCCGGGTCGTCACATATTTGCCTACGCCCGAGCAAAGCGCCTATCGGTTTTCCTA
TCACGAGCAAGGCTCGCCCGACAAAGAACCGACCAGCGGCCATACCCTGATCATGGGACGACCCGGGTCGGGCAAATCGG
TGCTGTCGGCCTTCCTGATGACCCAAGCCCGTCGAGCAGGGGCGCGGATCTTCGTCTTCGATTACCGTCTTGGTATGGAG
ATGGCGGTCCGCGCGAATGGCGGGCGCTACGCGTCCCTGAACGCAGGCCAGCCCACGGGTCTCAATCCGCTCTGGACCGA
AACCGATGCGCGGGGGACCGCCTGGCTCTCGGACTGGCTTGCCACGCTTCTCTACCGCGCCGACAAGCCCCTGACGCCCG
CACAGACCAACCGCATCCAGGAAGTCGTGCGCCAGAATGCCCAGGCCTCCAATCCGGCCCTGCGGAACTGGCGGGATTTC
GCATCGCTTTTTGTGTCCACCGATGATGGCGGCGATCTGCACCAGCGCCTGCTCGAATGGTCTGAGGACGGCCGCTATGG
CTGGATCTTCGGGCAGAGCCTCGAGGACACCTTCTCGCTCAAAGGCGATGTCGTGGGCTTCGATCTGACCGGCATTCTCG
ACAGCGAGGCCGACAAGGAGCGGATGGCGGTTCTCTCCTATCTTTTCCGCCGGGTCGAACGCGAGATCGAGGATCGCCGC
CCCACCATCATCGTGATCGACGAGGCCTGGAAGGCCCTCGATAACGCGTATTTCGCCGAGCGGCTGTCGAACTGGCTGGT
GACCGCGCGCAAGCAGAACACCGTCGCGGTGATGATGACGCAATACGCGAGCCAGCTCGAGCGCACCCGGACCGGCAAGA
CCATCGTCGAGGCCGTGCCGACGCAGATCCTGCTGCCCAATATCCGCGCGCAGCCTGCGGATTACGCCATGCTGAACCTC
ACGGAGAAGGAACTCGACGTCCTTCTCAACACGGGCAGCAACAGCCGCCTGGCGCTGATCCGCGACGATCAGGGCTCGAT
CGTCGTCGATGCCGATCTGAGCGCCCTTGGTCCCACGCTCACCATCCTCGGCGGCATGGAGAAAGGCGAGGCGCTCGTCG
GCGCCGATTACCGCGACCGCCCAGACTTCTGGAGGCTTTCATGA

Upstream 100 bases:

>100_bases
AAGGCCGCGGACTGGGATCCGCGTTTCATCGACGTGATGATGACGGCGCTGCAAGAGACGCCGCCCACGCGGAACCGCTC
CATCCACGGCGGGGACAGCT

Downstream 100 bases:

>100_bases
TCCGTATTCTTATCACTTTGGCTGCGCTCGGCGCGCTGACCTCCTGCACCCAGTATCGGGAGCCGCAGGCCAATTGCTTT
TCCTTTCTCGCCTCGACGGC

Product: CagE TrbE VirB component of type IV transporter system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 787; Mature: 786

Protein sequence:

>787_residues
MPREAALDLRTMTPEWYARETRLAHMLPYVSLVDHQTVRTRVNELFQCIRLDGVNSYTTDDAYLDKVTALFARIIAQLGP
EFSYYVHKVSKAITPDLEPLREDSFAGEVDRLWRKKLETSGLRDKTLTLTIIHRPPPKSVLPFLNRSAPDRLKEATEKRL
RRLGEAVNVFLSGLTELNPRVLSAKSGELIGFLGALNTGQELPLYPANTYGFLSFNVANTRVTFQGDHFELSEGVVGHRY
GKSFTIGEYSEGTSCTMFDMLNLPVDMIVTHSFTPINSNLMAGRIKRQKRQMQASQDAALSLLEALDIAADDLEAKRQSF
GEHHMVVTLFCETLDELQTLSAEIVNAAATEGVKMIGERVAAKAHYLSQHPGNQPKRVRASAVTNRNFADFAAFHRTQLG
KPAALTPWGRVVTYLPTPEQSAYRFSYHEQGSPDKEPTSGHTLIMGRPGSGKSVLSAFLMTQARRAGARIFVFDYRLGME
MAVRANGGRYASLNAGQPTGLNPLWTETDARGTAWLSDWLATLLYRADKPLTPAQTNRIQEVVRQNAQASNPALRNWRDF
ASLFVSTDDGGDLHQRLLEWSEDGRYGWIFGQSLEDTFSLKGDVVGFDLTGILDSEADKERMAVLSYLFRRVEREIEDRR
PTIIVIDEAWKALDNAYFAERLSNWLVTARKQNTVAVMMTQYASQLERTRTGKTIVEAVPTQILLPNIRAQPADYAMLNL
TEKELDVLLNTGSNSRLALIRDDQGSIVVDADLSALGPTLTILGGMEKGEALVGADYRDRPDFWRLS

Sequences:

>Translated_787_residues
MPREAALDLRTMTPEWYARETRLAHMLPYVSLVDHQTVRTRVNELFQCIRLDGVNSYTTDDAYLDKVTALFARIIAQLGP
EFSYYVHKVSKAITPDLEPLREDSFAGEVDRLWRKKLETSGLRDKTLTLTIIHRPPPKSVLPFLNRSAPDRLKEATEKRL
RRLGEAVNVFLSGLTELNPRVLSAKSGELIGFLGALNTGQELPLYPANTYGFLSFNVANTRVTFQGDHFELSEGVVGHRY
GKSFTIGEYSEGTSCTMFDMLNLPVDMIVTHSFTPINSNLMAGRIKRQKRQMQASQDAALSLLEALDIAADDLEAKRQSF
GEHHMVVTLFCETLDELQTLSAEIVNAAATEGVKMIGERVAAKAHYLSQHPGNQPKRVRASAVTNRNFADFAAFHRTQLG
KPAALTPWGRVVTYLPTPEQSAYRFSYHEQGSPDKEPTSGHTLIMGRPGSGKSVLSAFLMTQARRAGARIFVFDYRLGME
MAVRANGGRYASLNAGQPTGLNPLWTETDARGTAWLSDWLATLLYRADKPLTPAQTNRIQEVVRQNAQASNPALRNWRDF
ASLFVSTDDGGDLHQRLLEWSEDGRYGWIFGQSLEDTFSLKGDVVGFDLTGILDSEADKERMAVLSYLFRRVEREIEDRR
PTIIVIDEAWKALDNAYFAERLSNWLVTARKQNTVAVMMTQYASQLERTRTGKTIVEAVPTQILLPNIRAQPADYAMLNL
TEKELDVLLNTGSNSRLALIRDDQGSIVVDADLSALGPTLTILGGMEKGEALVGADYRDRPDFWRLS
>Mature_786_residues
PREAALDLRTMTPEWYARETRLAHMLPYVSLVDHQTVRTRVNELFQCIRLDGVNSYTTDDAYLDKVTALFARIIAQLGPE
FSYYVHKVSKAITPDLEPLREDSFAGEVDRLWRKKLETSGLRDKTLTLTIIHRPPPKSVLPFLNRSAPDRLKEATEKRLR
RLGEAVNVFLSGLTELNPRVLSAKSGELIGFLGALNTGQELPLYPANTYGFLSFNVANTRVTFQGDHFELSEGVVGHRYG
KSFTIGEYSEGTSCTMFDMLNLPVDMIVTHSFTPINSNLMAGRIKRQKRQMQASQDAALSLLEALDIAADDLEAKRQSFG
EHHMVVTLFCETLDELQTLSAEIVNAAATEGVKMIGERVAAKAHYLSQHPGNQPKRVRASAVTNRNFADFAAFHRTQLGK
PAALTPWGRVVTYLPTPEQSAYRFSYHEQGSPDKEPTSGHTLIMGRPGSGKSVLSAFLMTQARRAGARIFVFDYRLGMEM
AVRANGGRYASLNAGQPTGLNPLWTETDARGTAWLSDWLATLLYRADKPLTPAQTNRIQEVVRQNAQASNPALRNWRDFA
SLFVSTDDGGDLHQRLLEWSEDGRYGWIFGQSLEDTFSLKGDVVGFDLTGILDSEADKERMAVLSYLFRRVEREIEDRRP
TIIVIDEAWKALDNAYFAERLSNWLVTARKQNTVAVMMTQYASQLERTRTGKTIVEAVPTQILLPNIRAQPADYAMLNLT
EKELDVLLNTGSNSRLALIRDDQGSIVVDADLSALGPTLTILGGMEKGEALVGADYRDRPDFWRLS

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 88035; Mature: 87904

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPREAALDLRTMTPEWYARETRLAHMLPYVSLVDHQTVRTRVNELFQCIRLDGVNSYTTD
CCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DAYLDKVTALFARIIAQLGPEFSYYVHKVSKAITPDLEPLREDSFAGEVDRLWRKKLETS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHC
GLRDKTLTLTIIHRPPPKSVLPFLNRSAPDRLKEATEKRLRRLGEAVNVFLSGLTELNPR
CCCCCEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VLSAKSGELIGFLGALNTGQELPLYPANTYGFLSFNVANTRVTFQGDHFELSEGVVGHRY
EEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEECEEEEEECCCEECCCCCEECCC
GKSFTIGEYSEGTSCTMFDMLNLPVDMIVTHSFTPINSNLMAGRIKRQKRQMQASQDAAL
CCEEECCCCCCCCCEEEHHHHCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLLEALDIAADDLEAKRQSFGEHHMVVTLFCETLDELQTLSAEIVNAAATEGVKMIGERV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKAHYLSQHPGNQPKRVRASAVTNRNFADFAAFHRTQLGKPAALTPWGRVVTYLPTPEQ
HHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCC
SAYRFSYHEQGSPDKEPTSGHTLIMGRPGSGKSVLSAFLMTQARRAGARIFVFDYRLGME
CCEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCE
MAVRANGGRYASLNAGQPTGLNPLWTETDARGTAWLSDWLATLLYRADKPLTPAQTNRIQ
EEEEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EVVRQNAQASNPALRNWRDFASLFVSTDDGGDLHQRLLEWSEDGRYGWIFGQSLEDTFSL
HHHHHCCCCCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHCC
KGDVVGFDLTGILDSEADKERMAVLSYLFRRVEREIEDRRPTIIVIDEAWKALDNAYFAE
CCCEEEEEEHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHH
RLSNWLVTARKQNTVAVMMTQYASQLERTRTGKTIVEAVPTQILLPNIRAQPADYAMLNL
HHHHHHEEECCCCCEEHHHHHHHHHHHHHHCCCHHHHHCCCCEECCCCCCCCCCEEEEEC
TEKELDVLLNTGSNSRLALIRDDQGSIVVDADLSALGPTLTILGGMEKGEALVGADYRDR
CHHHHHEEEECCCCCEEEEEECCCCCEEEECCHHHCCCEEEEECCCCCCCEEECCCCCCC
PDFWRLS
CCCCCCC
>Mature Secondary Structure 
PREAALDLRTMTPEWYARETRLAHMLPYVSLVDHQTVRTRVNELFQCIRLDGVNSYTTD
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DAYLDKVTALFARIIAQLGPEFSYYVHKVSKAITPDLEPLREDSFAGEVDRLWRKKLETS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHC
GLRDKTLTLTIIHRPPPKSVLPFLNRSAPDRLKEATEKRLRRLGEAVNVFLSGLTELNPR
CCCCCEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VLSAKSGELIGFLGALNTGQELPLYPANTYGFLSFNVANTRVTFQGDHFELSEGVVGHRY
EEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEECEEEEEECCCEECCCCCEECCC
GKSFTIGEYSEGTSCTMFDMLNLPVDMIVTHSFTPINSNLMAGRIKRQKRQMQASQDAAL
CCEEECCCCCCCCCEEEHHHHCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLLEALDIAADDLEAKRQSFGEHHMVVTLFCETLDELQTLSAEIVNAAATEGVKMIGERV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKAHYLSQHPGNQPKRVRASAVTNRNFADFAAFHRTQLGKPAALTPWGRVVTYLPTPEQ
HHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCC
SAYRFSYHEQGSPDKEPTSGHTLIMGRPGSGKSVLSAFLMTQARRAGARIFVFDYRLGME
CCEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCE
MAVRANGGRYASLNAGQPTGLNPLWTETDARGTAWLSDWLATLLYRADKPLTPAQTNRIQ
EEEEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EVVRQNAQASNPALRNWRDFASLFVSTDDGGDLHQRLLEWSEDGRYGWIFGQSLEDTFSL
HHHHHCCCCCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHCC
KGDVVGFDLTGILDSEADKERMAVLSYLFRRVEREIEDRRPTIIVIDEAWKALDNAYFAE
CCCEEEEEEHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHH
RLSNWLVTARKQNTVAVMMTQYASQLERTRTGKTIVEAVPTQILLPNIRAQPADYAMLNL
HHHHHHEEECCCCCEEHHHHHHHHHHHHHHCCCHHHHHCCCCEECCCCCCCCCCEEEEEC
TEKELDVLLNTGSNSRLALIRDDQGSIVVDADLSALGPTLTILGGMEKGEALVGADYRDR
CHHHHHEEEECCCCCEEEEEECCCCCEEEECCHHHCCCEEEEECCCCCCCEEECCCCCCC
PDFWRLS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10749166; 10882236; 7494028 [H]