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The map label for this gene is leuA-3

Identifier: 15899159

GI number: 15899159

Start: 2190850

End: 2191893

Strand: Reverse

Name: leuA-3

Synonym: SSO2407

Alternate gene names: 15899159

Gene position: 2191893-2190850 (Counterclockwise)

Preceding gene: 15899160

Following gene: 15899158

Centisome position: 73.25

GC content: 36.11

Gene sequence:

>1044_bases
ATGATAGCGAGAAAGTTAGCCGATTTAGGTGTTGATGTAATAGAAGCAGGTTTCCCTGCTTCTTCAGAAGGAGAATTCAT
AGCTACCAGAAAGATTTTTGAAGAGATAGGAGATCAAGTAGAAGTAATTGGATTATCTAGATCTAATAAGAACGATATAG
ATAAGACAATAAGTACTGGGATCTCGAGTATACACTTGTTCATAGCGACTTCCGAATTGCATATGAAATATAAATTAAAG
ATGACAAAGGAGGAGGTATTAAATAAGATTTATGAAAGTGTAAAATACGCAAAAGATCACGGTATGATAGTAGAGTTTAG
TCCAGAGGATGCAACGAGAACTGAAGAAGACTTCCTATTTACTGCAATTAGAACTGCAATAGAAGCAGGAGCCGATAGGA
TCAATATACCGGATACTGTAGGGACAATGCATCCTTTTAAGTACTATGATATGATAAAGAAAATTGTAAACTTCGTAGGT
GAGAGAATAATAGTAAGTGTTCATTGCCATAATGATTTCGGACTAGCAACAGCAAATTCTTTAGCTGGCGTGTATGCAGG
TGCCAGACAAGCTCATGTTACTGTAAATGGTATAGGTGAGAGAGCAGGAAACGCGTCATTAGAGGAAGTAGTAATGGGTA
TAAAGAAATTACTAAATTATGAGACAAATGTGAAAACGTGGAAATTATATGAGGTAAGTAGGTTTGTAGCTGAAATGACC
GGCGTACCAGTTCCCTATTTCAAGGCAATTGTCGGTGATAATGCATTTGGACACGAATCTGGGATACACGTTCATGGAGT
AATAGAAAACCCATTCACATACGAACCAATTTCACCAGAAGAAGTAGGTAATTTCAGAAGACTAGCACTAGGTAAACATA
GCGGAATACATGGGTTAAGAAAATTATTAGAAGAACAAGGAATATATCTATCAGACGATAAATTAAAGATAGTCCTAGCT
GAGATTAAGAAACTGGCAGAATCAGGTAATAAGGTAACAGTAGAGGATGCGAAAAACATTGCACTTAAATTAATGAATTC
TTAA

Upstream 100 bases:

>100_bases
ATGAGTATTCTCTACGTCTAAGAAAATTAGAATATTCGATACCACGTTAAGAGACGGCGAACAAGCTCCCGGTATAGATT
TAACAACAGAACAAAAGATA

Downstream 100 bases:

>100_bases
ACGATTGTATGGTACTAAAATATTTCGGACATTCTTGTGTTTTAATTGATGATAGAATATTAATTGATCCGCACGATGGA
GGAAGTATTGGTTTACCTAA

Product: 2-isopropylmalate synthase, putative (leuA-3)

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MIARKLADLGVDVIEAGFPASSEGEFIATRKIFEEIGDQVEVIGLSRSNKNDIDKTISTGISSIHLFIATSELHMKYKLK
MTKEEVLNKIYESVKYAKDHGMIVEFSPEDATRTEEDFLFTAIRTAIEAGADRINIPDTVGTMHPFKYYDMIKKIVNFVG
ERIIVSVHCHNDFGLATANSLAGVYAGARQAHVTVNGIGERAGNASLEEVVMGIKKLLNYETNVKTWKLYEVSRFVAEMT
GVPVPYFKAIVGDNAFGHESGIHVHGVIENPFTYEPISPEEVGNFRRLALGKHSGIHGLRKLLEEQGIYLSDDKLKIVLA
EIKKLAESGNKVTVEDAKNIALKLMNS

Sequences:

>Translated_347_residues
MIARKLADLGVDVIEAGFPASSEGEFIATRKIFEEIGDQVEVIGLSRSNKNDIDKTISTGISSIHLFIATSELHMKYKLK
MTKEEVLNKIYESVKYAKDHGMIVEFSPEDATRTEEDFLFTAIRTAIEAGADRINIPDTVGTMHPFKYYDMIKKIVNFVG
ERIIVSVHCHNDFGLATANSLAGVYAGARQAHVTVNGIGERAGNASLEEVVMGIKKLLNYETNVKTWKLYEVSRFVAEMT
GVPVPYFKAIVGDNAFGHESGIHVHGVIENPFTYEPISPEEVGNFRRLALGKHSGIHGLRKLLEEQGIYLSDDKLKIVLA
EIKKLAESGNKVTVEDAKNIALKLMNS
>Mature_347_residues
MIARKLADLGVDVIEAGFPASSEGEFIATRKIFEEIGDQVEVIGLSRSNKNDIDKTISTGISSIHLFIATSELHMKYKLK
MTKEEVLNKIYESVKYAKDHGMIVEFSPEDATRTEEDFLFTAIRTAIEAGADRINIPDTVGTMHPFKYYDMIKKIVNFVG
ERIIVSVHCHNDFGLATANSLAGVYAGARQAHVTVNGIGERAGNASLEEVVMGIKKLLNYETNVKTWKLYEVSRFVAEMT
GVPVPYFKAIVGDNAFGHESGIHVHGVIENPFTYEPISPEEVGNFRRLALGKHSGIHGLRKLLEEQGIYLSDDKLKIVLA
EIKKLAESGNKVTVEDAKNIALKLMNS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=351, Percent_Identity=40.7407407407407, Blast_Score=233, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6320019, Length=332, Percent_Identity=30.7228915662651, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320071, Length=332, Percent_Identity=30.7228915662651, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324682, Length=316, Percent_Identity=28.7974683544304, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324225, Length=316, Percent_Identity=28.4810126582279, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU12_SULSO (Q97W36)

Other databases:

- EMBL:   AE006641
- PIR:   C90412
- RefSeq:   NP_343764.1
- ProteinModelPortal:   Q97W36
- SMR:   Q97W36
- GeneID:   1453881
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO2407
- NMPDR:   fig|273057.1.peg.2187
- HOGENOM:   HBG701974
- OMA:   SSTANVY
- PhylomeDB:   Q97W36
- BioCyc:   SSOL273057:SSO2407-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_A
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like

EC number: =2.3.3.13

Molecular weight: Translated: 38402; Mature: 38402

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARKLADLGVDVIEAGFPASSEGEFIATRKIFEEIGDQVEVIGLSRSNKNDIDKTISTG
CCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHC
ISSIHLFIATSELHMKYKLKMTKEEVLNKIYESVKYAKDHGMIVEFSPEDATRTEEDFLF
CCEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
TAIRTAIEAGADRINIPDTVGTMHPFKYYDMIKKIVNFVGERIIVSVHCHNDFGLATANS
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHH
LAGVYAGARQAHVTVNGIGERAGNASLEEVVMGIKKLLNYETNVKTWKLYEVSRFVAEMT
HHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHH
GVPVPYFKAIVGDNAFGHESGIHVHGVIENPFTYEPISPEEVGNFRRLALGKHSGIHGLR
CCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHCCHHHHHHCCCCCHHHHH
KLLEEQGIYLSDDKLKIVLAEIKKLAESGNKVTVEDAKNIALKLMNS
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCEEEEECHHCCHHEEECC
>Mature Secondary Structure
MIARKLADLGVDVIEAGFPASSEGEFIATRKIFEEIGDQVEVIGLSRSNKNDIDKTISTG
CCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHC
ISSIHLFIATSELHMKYKLKMTKEEVLNKIYESVKYAKDHGMIVEFSPEDATRTEEDFLF
CCEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
TAIRTAIEAGADRINIPDTVGTMHPFKYYDMIKKIVNFVGERIIVSVHCHNDFGLATANS
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHH
LAGVYAGARQAHVTVNGIGERAGNASLEEVVMGIKKLLNYETNVKTWKLYEVSRFVAEMT
HHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHH
GVPVPYFKAIVGDNAFGHESGIHVHGVIENPFTYEPISPEEVGNFRRLALGKHSGIHGLR
CCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHCCHHHHHHCCCCCHHHHH
KLLEEQGIYLSDDKLKIVLAEIKKLAESGNKVTVEDAKNIALKLMNS
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCEEEEECHHCCHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726