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The map label for this gene is thiD-2 [H]

Identifier: 15899145

GI number: 15899145

Start: 2179413

End: 2180783

Strand: Reverse

Name: thiD-2 [H]

Synonym: SSO2393

Alternate gene names: 15899145

Gene position: 2180783-2179413 (Counterclockwise)

Preceding gene: 15899146

Following gene: 15899144

Centisome position: 72.88

GC content: 35.3

Gene sequence:

>1371_bases
TTGGGTAGTAGCAGGGAATACGAGAGTGAAAAAACAATGATTAAACCTGTTGGTATGAGCATAGCTGGATTGGATACTGG
AAATGGGGCTGGTGGAGAGACTGACTTAAGAGTTTTTGAAGTATTAGGAATCCACGGAGTTTTTGCTATCACTGCGATAA
CAGCTCAAAGTACAAAAGGCATAAAAGATATCACTGTTGTTAATCCTGAGTTTCTTAAAAAGCAGATAGAAACTTTACTC
GAGGATTTCAAAGTGGAAGCTGTAAAGATAGGAATGATATATACTAAAGAACAGTTTCAGGTTGTAAATGAATTATTAAA
TGATTACTTTTTAGTAGCAGATCCGGTACTTTATGCGAAAGATGGAACTCCGCTGATTAGAGACATTGAAGAATATAAGA
AGATAATTTTGCCAAAAGTTAAAGTAATAACGCCAAATATAATAGAAGCATCTGCAATTAGCGGTGTCAAAATAGAAAAG
GAGAGCGATGTAGTAATTGTATGTAAGAAATTAAGGGATAGTTATAATATTCCCTACGTGATAATCAAGGGAGGCCATAG
TAAAGGAGATTATAGTTTCGATTATATGTGTAATGATGAAGGACTATACAAGATAGGATATAAAAGGTTGCAGGCAAAGG
ATACGCATGGTACTGGAAGCGTATTTGCAACTGCACTTACTGCAGAATACATAAAGATAAGGGATTTAAAATTGGCCTTT
AGGAAGGCTAGAGATTTCGTTCAAACCTCAATAGAATATGGACTTAACATAGGAAAAGGTATAGGACCAGTTAACGTAAG
TGTTGAAATTATGAAGAAGTCTATGAAATATGAAGCAGTTGAGGAAATGAGGAGATTTGCGGATTTTGCCGAAAATAACG
ATAGATTCTGGATTTTAATCCCTGAAGTACAATCAAATCTAGCACATAGTATAAAACCAGAATACGTTAGGGATCTAAAT
GATATTGCTACATTTAGGGGTAGAATAATAAGGAGATGGGATAAAAAGGTAATTGTTGGACATCCAGTGGTGTTTGGAAA
CCCTACCCATACAGCTCGAATGCTGTTGTCGCTCATTCTCAAGGGAAAGGATAGTACTTGCTTAATGAATATTAGATATG
ACGATAAGATAGTTGAGAGTTTTAAGAGAATTGGGTATGAAACAATTGAGATTAATAGGGAACTAGAACCTGCCCATGGA
GAAGGAAAGACAATGCAATGGATTATTGAGTATGTAAGTAGCGAATATGGTGGCATACCGAATGTAATTTATGATAAGGG
AACTAAGGGAAAGGAGGCAATGATTAGGTTTTGGACAAAAAACATGGAAGAGATGATAGAAGCTTTAGATAATTTATTAA
AAATGCTGTAA

Upstream 100 bases:

>100_bases
AGGGAAGGAAGGACGCTATCGACGCGGTAAAAGAAACATTGGAATTAGTTAAACATAGCACTGGAATTTGGAAGTTGGAA
ATAAGAGATGACGGAGAATA

Downstream 100 bases:

>100_bases
CTTCATTTTAATTTGAAATGAAACTAGAGAGATTGTTAGAGGGCGCCAAAAACTTAGTTAGAACCGGTTGGATGCAAAAC
GGAATTCCCTCAGCGATGGG

Product: phosphomethylpyrimidine kinase (thiD-2)

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MGSSREYESEKTMIKPVGMSIAGLDTGNGAGGETDLRVFEVLGIHGVFAITAITAQSTKGIKDITVVNPEFLKKQIETLL
EDFKVEAVKIGMIYTKEQFQVVNELLNDYFLVADPVLYAKDGTPLIRDIEEYKKIILPKVKVITPNIIEASAISGVKIEK
ESDVVIVCKKLRDSYNIPYVIIKGGHSKGDYSFDYMCNDEGLYKIGYKRLQAKDTHGTGSVFATALTAEYIKIRDLKLAF
RKARDFVQTSIEYGLNIGKGIGPVNVSVEIMKKSMKYEAVEEMRRFADFAENNDRFWILIPEVQSNLAHSIKPEYVRDLN
DIATFRGRIIRRWDKKVIVGHPVVFGNPTHTARMLLSLILKGKDSTCLMNIRYDDKIVESFKRIGYETIEINRELEPAHG
EGKTMQWIIEYVSSEYGGIPNVIYDKGTKGKEAMIRFWTKNMEEMIEALDNLLKML

Sequences:

>Translated_456_residues
MGSSREYESEKTMIKPVGMSIAGLDTGNGAGGETDLRVFEVLGIHGVFAITAITAQSTKGIKDITVVNPEFLKKQIETLL
EDFKVEAVKIGMIYTKEQFQVVNELLNDYFLVADPVLYAKDGTPLIRDIEEYKKIILPKVKVITPNIIEASAISGVKIEK
ESDVVIVCKKLRDSYNIPYVIIKGGHSKGDYSFDYMCNDEGLYKIGYKRLQAKDTHGTGSVFATALTAEYIKIRDLKLAF
RKARDFVQTSIEYGLNIGKGIGPVNVSVEIMKKSMKYEAVEEMRRFADFAENNDRFWILIPEVQSNLAHSIKPEYVRDLN
DIATFRGRIIRRWDKKVIVGHPVVFGNPTHTARMLLSLILKGKDSTCLMNIRYDDKIVESFKRIGYETIEINRELEPAHG
EGKTMQWIIEYVSSEYGGIPNVIYDKGTKGKEAMIRFWTKNMEEMIEALDNLLKML
>Mature_455_residues
GSSREYESEKTMIKPVGMSIAGLDTGNGAGGETDLRVFEVLGIHGVFAITAITAQSTKGIKDITVVNPEFLKKQIETLLE
DFKVEAVKIGMIYTKEQFQVVNELLNDYFLVADPVLYAKDGTPLIRDIEEYKKIILPKVKVITPNIIEASAISGVKIEKE
SDVVIVCKKLRDSYNIPYVIIKGGHSKGDYSFDYMCNDEGLYKIGYKRLQAKDTHGTGSVFATALTAEYIKIRDLKLAFR
KARDFVQTSIEYGLNIGKGIGPVNVSVEIMKKSMKYEAVEEMRRFADFAENNDRFWILIPEVQSNLAHSIKPEYVRDLND
IATFRGRIIRRWDKKVIVGHPVVFGNPTHTARMLLSLILKGKDSTCLMNIRYDDKIVESFKRIGYETIEINRELEPAHGE
GKTMQWIIEYVSSEYGGIPNVIYDKGTKGKEAMIRFWTKNMEEMIEALDNLLKML

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=255, Percent_Identity=30.1960784313725, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6325378, Length=281, Percent_Identity=26.6903914590747, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6324997, Length=270, Percent_Identity=28.5185185185185, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324517, Length=339, Percent_Identity=26.2536873156342, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 51662; Mature: 51530

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSSREYESEKTMIKPVGMSIAGLDTGNGAGGETDLRVFEVLGIHGVFAITAITAQSTKG
CCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCC
IKDITVVNPEFLKKQIETLLEDFKVEAVKIGMIYTKEQFQVVNELLNDYFLVADPVLYAK
CCEEEEECHHHHHHHHHHHHHHCCEEEEEEEEEEEHHHHHHHHHHHHCEEEEECCEEEEC
DGTPLIRDIEEYKKIILPKVKVITPNIIEASAISGVKIEKESDVVIVCKKLRDSYNIPYV
CCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEECCCCEEEEEEHHHCCCCCCEE
IIKGGHSKGDYSFDYMCNDEGLYKIGYKRLQAKDTHGTGSVFATALTAEYIKIRDLKLAF
EEECCCCCCCEEEEEEECCCCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RKARDFVQTSIEYGLNIGKGIGPVNVSVEIMKKSMKYEAVEEMRRFADFAENNDRFWILI
HHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
PEVQSNLAHSIKPEYVRDLNDIATFRGRIIRRWDKKVIVGHPVVFGNPTHTARMLLSLIL
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCCHHHHHHHHHHH
KGKDSTCLMNIRYDDKIVESFKRIGYETIEINRELEPAHGEGKTMQWIIEYVSSEYGGIP
CCCCCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCC
NVIYDKGTKGKEAMIRFWTKNMEEMIEALDNLLKML
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
GSSREYESEKTMIKPVGMSIAGLDTGNGAGGETDLRVFEVLGIHGVFAITAITAQSTKG
CCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCC
IKDITVVNPEFLKKQIETLLEDFKVEAVKIGMIYTKEQFQVVNELLNDYFLVADPVLYAK
CCEEEEECHHHHHHHHHHHHHHCCEEEEEEEEEEEHHHHHHHHHHHHCEEEEECCEEEEC
DGTPLIRDIEEYKKIILPKVKVITPNIIEASAISGVKIEKESDVVIVCKKLRDSYNIPYV
CCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEECCCCEEEEEEHHHCCCCCCEE
IIKGGHSKGDYSFDYMCNDEGLYKIGYKRLQAKDTHGTGSVFATALTAEYIKIRDLKLAF
EEECCCCCCCEEEEEEECCCCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RKARDFVQTSIEYGLNIGKGIGPVNVSVEIMKKSMKYEAVEEMRRFADFAENNDRFWILI
HHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
PEVQSNLAHSIKPEYVRDLNDIATFRGRIIRRWDKKVIVGHPVVFGNPTHTARMLLSLIL
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCCHHHHHHHHHHH
KGKDSTCLMNIRYDDKIVESFKRIGYETIEINRELEPAHGEGKTMQWIIEYVSSEYGGIP
CCCCCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCC
NVIYDKGTKGKEAMIRFWTKNMEEMIEALDNLLKML
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]