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The map label for this gene is icaA [H]

Identifier: 15899142

GI number: 15899142

Start: 2177035

End: 2178315

Strand: Reverse

Name: icaA [H]

Synonym: SSO2389

Alternate gene names: 15899142

Gene position: 2178315-2177035 (Counterclockwise)

Preceding gene: 15899143

Following gene: 15899141

Centisome position: 72.8

GC content: 30.84

Gene sequence:

>1281_bases
ATGATAAGGGAAATTTTTGAAATTTTGCTTTTTATATCATCATTTTTTACATCTTTATGGATACTCCTTCAAGCATTCTA
CTATAAAGTTTCTAATCAAAACATTATACAATTTTCTACTAAAAATGATAAAAATTCTAACAAAAGAATAGACATAATCG
TTGCAATAAAAGACGAAGATGAAAAGACGATTAAAGAACTAATTAATAACCTATCGGGATTGGACTATAGATTTTACAAA
GTTATTATAGTATCTGATGATACAGAGGAGACTTTTAAAAAAATTATAGAATCACTAGATAAACTTCCGGACAATTTCGT
AATTATAAGGAGACCAGAAAACAAGGGAAGAAAAGCTGGAGCACTAAACTTCGCCACTAATATTTCTGATGCTGAAATGT
TAGTGTATCTGGATGCAGAAGCCAGGGTCGAAAAGGACTTTTTACGTAAAATTTCTCAACTTGACTACGATGCGGTTGCG
TTTAGATTAAAAGTTAGAGATGTTAATACACAAGTTCAAAAGATATACTCATATACCAATGAATTTGTAATGAACGCATT
ATTCAAGGCTAGAGACAAGTTAGGTCTAATAATATTTGCAAATGGTTCAGCATTCGGAATAAAGAGAGATATTTTAAGGA
AGATAGGTGGATGGAAAGAAAATAGCGTAGCAGAAGACTTAGAGCTAGGTATTAGACTTGCTCTGAGTAATATTAAAGTA
AAATACGTTGATGACATCACAGTTTATACCTTAGCTCCCTATACCCATACTGATTTATATAACCAAATTAAAAGATGGGC
TTATGGTTCTGGAGAATTGATCTCTTACAGCATGAGATTGTTTAAATTAGGAATAAGGGGAATTGAGGGATTTATATACT
CACAACAGTGGGGAATTTACCCCCTATACCTACTACTATTTCTTATTATTATCTCAATACAGTTTATATTAAATATAAAC
TACTTTTATGTCTTTACCTCACTAATCCCAATACTAGTCTCGAATGGAATTTACATAGCTCTGATAAAACCTAAGGGAGA
TTATAAAAGTGGCATTGTAACCCTAATTGCTTCTCTTATCGGCTACATTCAAGGAATATTCAAAGTCAGGTTTAAATGGA
AAGTTACACCCAAAAGCCTAGTTGGGAAAGAAGAAGAAATCTTGAGTATAAAAATATTAGGGATTATTCTTGCGATAATG
GCATATATTAATAGTCTTTTCAATAACACAATTTCATCTTTATTAATAATTTTGTTTTCACTTATTCTTTTAACTCTATA
G

Upstream 100 bases:

>100_bases
AAGAACTAGAGCCAAATAAATGGGTAAAGATTAGCGGATGGGGAAGCGTAGCTGAGGCTAAGGAAAGAATTAAAAAAGCG
ATTGAGAGAAAGAAACAAGG

Downstream 100 bases:

>100_bases
TTTTTGTCCTAGCCATTGCTTGAGCCTCTTTTAGAGCTTCTTCGGGTTTTAAGCTGTATTTTATTATATAGCTCTTCACA
AGCTTATAACTCTCCTCATT

Product: glucosaminyltransferase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MIREIFEILLFISSFFTSLWILLQAFYYKVSNQNIIQFSTKNDKNSNKRIDIIVAIKDEDEKTIKELINNLSGLDYRFYK
VIIVSDDTEETFKKIIESLDKLPDNFVIIRRPENKGRKAGALNFATNISDAEMLVYLDAEARVEKDFLRKISQLDYDAVA
FRLKVRDVNTQVQKIYSYTNEFVMNALFKARDKLGLIIFANGSAFGIKRDILRKIGGWKENSVAEDLELGIRLALSNIKV
KYVDDITVYTLAPYTHTDLYNQIKRWAYGSGELISYSMRLFKLGIRGIEGFIYSQQWGIYPLYLLLFLIIISIQFILNIN
YFYVFTSLIPILVSNGIYIALIKPKGDYKSGIVTLIASLIGYIQGIFKVRFKWKVTPKSLVGKEEEILSIKILGIILAIM
AYINSLFNNTISSLLIILFSLILLTL

Sequences:

>Translated_426_residues
MIREIFEILLFISSFFTSLWILLQAFYYKVSNQNIIQFSTKNDKNSNKRIDIIVAIKDEDEKTIKELINNLSGLDYRFYK
VIIVSDDTEETFKKIIESLDKLPDNFVIIRRPENKGRKAGALNFATNISDAEMLVYLDAEARVEKDFLRKISQLDYDAVA
FRLKVRDVNTQVQKIYSYTNEFVMNALFKARDKLGLIIFANGSAFGIKRDILRKIGGWKENSVAEDLELGIRLALSNIKV
KYVDDITVYTLAPYTHTDLYNQIKRWAYGSGELISYSMRLFKLGIRGIEGFIYSQQWGIYPLYLLLFLIIISIQFILNIN
YFYVFTSLIPILVSNGIYIALIKPKGDYKSGIVTLIASLIGYIQGIFKVRFKWKVTPKSLVGKEEEILSIKILGIILAIM
AYINSLFNNTISSLLIILFSLILLTL
>Mature_426_residues
MIREIFEILLFISSFFTSLWILLQAFYYKVSNQNIIQFSTKNDKNSNKRIDIIVAIKDEDEKTIKELINNLSGLDYRFYK
VIIVSDDTEETFKKIIESLDKLPDNFVIIRRPENKGRKAGALNFATNISDAEMLVYLDAEARVEKDFLRKISQLDYDAVA
FRLKVRDVNTQVQKIYSYTNEFVMNALFKARDKLGLIIFANGSAFGIKRDILRKIGGWKENSVAEDLELGIRLALSNIKV
KYVDDITVYTLAPYTHTDLYNQIKRWAYGSGELISYSMRLFKLGIRGIEGFIYSQQWGIYPLYLLLFLIIISIQFILNIN
YFYVFTSLIPILVSNGIYIALIKPKGDYKSGIVTLIASLIGYIQGIFKVRFKWKVTPKSLVGKEEEILSIKILGIILAIM
AYINSLFNNTISSLLIILFSLILLTL

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=288, Percent_Identity=23.9583333333333, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 49035; Mature: 49035

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREIFEILLFISSFFTSLWILLQAFYYKVSNQNIIQFSTKNDKNSNKRIDIIVAIKDED
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEEECCC
EKTIKELINNLSGLDYRFYKVIIVSDDTEETFKKIIESLDKLPDNFVIIRRPENKGRKAG
HHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
ALNFATNISDAEMLVYLDAEARVEKDFLRKISQLDYDAVAFRLKVRDVNTQVQKIYSYTN
CEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHHEEEEEEEEHHHHHHHHHHHHHH
EFVMNALFKARDKLGLIIFANGSAFGIKRDILRKIGGWKENSVAEDLELGIRLALSNIKV
HHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHEEEECCEE
KYVDDITVYTLAPYTHTDLYNQIKRWAYGSGELISYSMRLFKLGIRGIEGFIYSQQWGIY
EEECCEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCHH
PLYLLLFLIIISIQFILNINYFYVFTSLIPILVSNGIYIALIKPKGDYKSGIVTLIASLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHH
GYIQGIFKVRFKWKVTPKSLVGKEEEILSIKILGIILAIMAYINSLFNNTISSLLIILFS
HHHHHHHEEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILLTL
HHHHCC
>Mature Secondary Structure
MIREIFEILLFISSFFTSLWILLQAFYYKVSNQNIIQFSTKNDKNSNKRIDIIVAIKDED
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEEECCC
EKTIKELINNLSGLDYRFYKVIIVSDDTEETFKKIIESLDKLPDNFVIIRRPENKGRKAG
HHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
ALNFATNISDAEMLVYLDAEARVEKDFLRKISQLDYDAVAFRLKVRDVNTQVQKIYSYTN
CEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHHEEEEEEEEHHHHHHHHHHHHHH
EFVMNALFKARDKLGLIIFANGSAFGIKRDILRKIGGWKENSVAEDLELGIRLALSNIKV
HHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHEEEECCEE
KYVDDITVYTLAPYTHTDLYNQIKRWAYGSGELISYSMRLFKLGIRGIEGFIYSQQWGIY
EEECCEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCHH
PLYLLLFLIIISIQFILNINYFYVFTSLIPILVSNGIYIALIKPKGDYKSGIVTLIASLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHH
GYIQGIFKVRFKWKVTPKSLVGKEEEILSIKILGIILAIMAYINSLFNNTISSLLIILFS
HHHHHHHEEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILLTL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]