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The map label for this gene is cbiF [H]

Identifier: 15899064

GI number: 15899064

Start: 2106526

End: 2107314

Strand: Reverse

Name: cbiF [H]

Synonym: SSO2299

Alternate gene names: 15899064

Gene position: 2107314-2106526 (Counterclockwise)

Preceding gene: 15899065

Following gene: 15899063

Centisome position: 70.43

GC content: 39.92

Gene sequence:

>789_bases
ATGATAGGAAAAGTGGTTTTCATAGGGTCTGGTCCAGGAGATCCAGAGCTAATTACGATAAAAGCGAAGAAGTATATAGA
AACAGCTGACGTAATAGTCTATGCGGGTTCCCTTGTAAATCCAGAAATATTAAAGTGGTCTCGTAAGGACGCTGAAGTAT
ACAATAGTTCATCACTTACTTTAAATGAGATAGTGGAAATAATGGTGAAGAAGGCAAGTGAAGGAAAACTGGTTGCTAGG
CTCAAATCTGGAGATTCATCAATATACGGAGCATTATTTGAAGAGATGTGGGCATTGGAGGCTGCGGGGATTCCATTTGA
AGTTATACCGGGTATCACTGCAGCTATTGCCGCAGCTTCTGTAATACCGATTGAGTTAACAGTCCCTAAGCTTTCCCAAA
CTGTCATTATAACAAGGGCTTCACTAAGAGTCCCAATGCAAGGTTCCATAAAGGATTTCGCCAAAATGGTAAAGATTGGA
GCTACTATGGTAATATATACTGGAATACATATCATTGACAGAGTTGTTAATGATCTAAAGGAGGGAGGATTGACTGACGA
TACTCCAGTAATAGTGGTCTATCGTGCTACTTGGCCGGAGCAAAAGATAATCAAAGGAACTTTGGCTGATATCGTAAGTA
AGGTTAGAGAAGCTAAGATATATAGGGATTCAGTAATAATAGTAGGACTTTCAGCAGATCCCCAACAGATCAAGAATATG
GTAAGATCTAGTGTATACGATCCCAAACATAATCACTCGTATAGGCCTTGGAAAGTGGAGGAAGACTAA

Upstream 100 bases:

>100_bases
CATGAGTGATGAAAAATTAGTTAATATGGAAAGGGAGATTATTAATGATAAGGATTACTTTTCGATGATAATAGCTTTGA
GAAAGAAAAGGTGATTATAA

Downstream 100 bases:

>100_bases
CAATGATAGAAAACTTATGGCGAGGAATTTGCATAATATCTGCCTCTGAAGACGCTTTTAGCGCAGGCGAAACTATTAAG
GAGAAGTTGAAAAGTTTCGA

Product: cobalamin biosynthesis precorrin-3 methylase, putative (cbiF)

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MIGKVVFIGSGPGDPELITIKAKKYIETADVIVYAGSLVNPEILKWSRKDAEVYNSSSLTLNEIVEIMVKKASEGKLVAR
LKSGDSSIYGALFEEMWALEAAGIPFEVIPGITAAIAAASVIPIELTVPKLSQTVIITRASLRVPMQGSIKDFAKMVKIG
ATMVIYTGIHIIDRVVNDLKEGGLTDDTPVIVVYRATWPEQKIIKGTLADIVSKVREAKIYRDSVIIVGLSADPQQIKNM
VRSSVYDPKHNHSYRPWKVEED

Sequences:

>Translated_262_residues
MIGKVVFIGSGPGDPELITIKAKKYIETADVIVYAGSLVNPEILKWSRKDAEVYNSSSLTLNEIVEIMVKKASEGKLVAR
LKSGDSSIYGALFEEMWALEAAGIPFEVIPGITAAIAAASVIPIELTVPKLSQTVIITRASLRVPMQGSIKDFAKMVKIG
ATMVIYTGIHIIDRVVNDLKEGGLTDDTPVIVVYRATWPEQKIIKGTLADIVSKVREAKIYRDSVIIVGLSADPQQIKNM
VRSSVYDPKHNHSYRPWKVEED
>Mature_262_residues
MIGKVVFIGSGPGDPELITIKAKKYIETADVIVYAGSLVNPEILKWSRKDAEVYNSSSLTLNEIVEIMVKKASEGKLVAR
LKSGDSSIYGALFEEMWALEAAGIPFEVIPGITAAIAAASVIPIELTVPKLSQTVIITRASLRVPMQGSIKDFAKMVKIG
ATMVIYTGIHIIDRVVNDLKEGGLTDDTPVIVVYRATWPEQKIIKGTLADIVSKVREAKIYRDSVIIVGLSADPQQIKNM
VRSSVYDPKHNHSYRPWKVEED

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=233, Percent_Identity=34.7639484978541, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322922, Length=234, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28723; Mature: 28723

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGKVVFIGSGPGDPELITIKAKKYIETADVIVYAGSLVNPEILKWSRKDAEVYNSSSLT
CCCEEEEEECCCCCCCEEEEEHHHHHHHHCEEEEECCCCCHHHHHCCCCHHHHHCCCCCC
LNEIVEIMVKKASEGKLVARLKSGDSSIYGALFEEMWALEAAGIPFEVIPGITAAIAAAS
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHH
VIPIELTVPKLSQTVIITRASLRVPMQGSIKDFAKMVKIGATMVIYTGIHIIDRVVNDLK
EEEEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHH
EGGLTDDTPVIVVYRATWPEQKIIKGTLADIVSKVREAKIYRDSVIIVGLSADPQQIKNM
HCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCHHHHHHH
VRSSVYDPKHNHSYRPWKVEED
HHHHCCCCCCCCCCCCEECCCC
>Mature Secondary Structure
MIGKVVFIGSGPGDPELITIKAKKYIETADVIVYAGSLVNPEILKWSRKDAEVYNSSSLT
CCCEEEEEECCCCCCCEEEEEHHHHHHHHCEEEEECCCCCHHHHHCCCCHHHHHCCCCCC
LNEIVEIMVKKASEGKLVARLKSGDSSIYGALFEEMWALEAAGIPFEVIPGITAAIAAAS
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHH
VIPIELTVPKLSQTVIITRASLRVPMQGSIKDFAKMVKIGATMVIYTGIHIIDRVVNDLK
EEEEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHH
EGGLTDDTPVIVVYRATWPEQKIIKGTLADIVSKVREAKIYRDSVIIVGLSADPQQIKNM
HCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCHHHHHHH
VRSSVYDPKHNHSYRPWKVEED
HHHHCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]