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The map label for this gene is cysS

Identifier: 15899047

GI number: 15899047

Start: 2089224

End: 2090636

Strand: Reverse

Name: cysS

Synonym: SSO2280

Alternate gene names: 15899047

Gene position: 2090636-2089224 (Counterclockwise)

Preceding gene: 15899048

Following gene: 15899045

Centisome position: 69.87

GC content: 35.46

Gene sequence:

>1413_bases
ATGGATTTTAGAATTAGGGTATACAATTCGCTTGGGAGAAAGCTAGAGGAATTTGGTACAGTAGAGCCGAATCTAGTGAA
AATGTACGTCTGCGGCCCTACAGTTTATGACTATGTTCATATAGGTCATGGAAGAACTTTCGTGGTATTTGACGCCATTT
CAAGATATTTAAGGTTAAGAGGCTATACCGTAATTAGAGTTCAGAATATTACAGACATTGATGACAAAATAATTAAAAAA
TCACAAGAGATTGGAAAAGATTGGAATGAAATCGTTGATTATTTCACAAAGGATTACTTAGATATGCTGTCTCAACTTAA
AGTCAAAATAGATATACACCCACGTGTAACACAGCATATAAGGGAAATCATTGATTTCGTACAGAGACTGATAGATAAGG
GACATGCATATGTAGCGCCTAGTGGTAGTGTGTACTTTGATGTTGATACTTATCCTAATTATGGGGAACTTTCAAACACT
AAAAAAGAAGAATGGAATCAAGGAGAAGAATTCGTCAAGGAGAAGAAGCATTCCTACGACTTCGCATTATGGAAAGCGTG
GAAGCCGGGTGAACCTTATTGGGAATCACCTTGGGGTAAGGGAAGACCTGGATGGCACATCGAATGTTCAACTATGTCAA
CTAGGTATCTAGGTGAAAGATTTGATATTCATGGAGGAGGAGCTGATTTGATATTTCCGCATCATGAAAATGAAAGAGCT
CAGACTGAGGCATTAATTGGGGAGAAATGGGTTACATACTGGGTTCATAGCGCTTTTGTAACCATAAGAAAAGAAAAGAT
GAGTAAATCCTTGGGTAATATAATACCATTAAATGAGGCTATAAAGAAATGGGGACCCTCGGTTTTGAGATATTGGTATT
TGACCTCTCATTATAGATCACCAATAGACTTTTCTGAGGAAGCCTTAGAGCAAGCAAAATCCGCTTTACAGAGAATAAAA
GATTCAATGGCAATAATTAGAGATGTAATATCTGAGGGACCTAAGTTCTATGTTAAAGATGATGATATCAAAGTATATAG
AGAGATACTTAATAATCTAAATAACTTCCATACAGCCATGAGTAATGATTTCGACACATCTACAGCCTTATCATATATAC
ACGAGATAGTAAGATTAGTATTCTCCACATTACAATATAGTAGAGATTTCCTTGGGGCTATGTTAGCCTTCGAAACCCTT
AAACAATTCAATGAAGTATTTGGAGTAATGGATGAAGAATTCTATCCCACATATGATAAGATGTATAAAATAATAGATGC
AGTTGTAGATATAAGAAATCAATTGAGACAGATGAAATTATACGAAATTTCAGATAAGATAAGGGAAGAATTACTAAAGG
CTGGAGTAAGGATATTAGACAGTAAGGATAAATCTACTTGGAGATTTGAATGA

Upstream 100 bases:

>100_bases
GGCTAAAAAACTTAACGAGAAACCAGATAAGCTGTATAACATACCCAAAGCTAGGCAACTAACTTCAAATTTATTTAAAA
TAAACTAGTGTGAACAGATA

Downstream 100 bases:

>100_bases
TGAAATATGGTGGCTTTTCTCCTCTAAAGTACATACTCAGCATGTCGTAGGTAGTTTTTGTAGTTTGTATAATATTTAAT
TTGTCAAAAGGTACATATTC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MDFRIRVYNSLGRKLEEFGTVEPNLVKMYVCGPTVYDYVHIGHGRTFVVFDAISRYLRLRGYTVIRVQNITDIDDKIIKK
SQEIGKDWNEIVDYFTKDYLDMLSQLKVKIDIHPRVTQHIREIIDFVQRLIDKGHAYVAPSGSVYFDVDTYPNYGELSNT
KKEEWNQGEEFVKEKKHSYDFALWKAWKPGEPYWESPWGKGRPGWHIECSTMSTRYLGERFDIHGGGADLIFPHHENERA
QTEALIGEKWVTYWVHSAFVTIRKEKMSKSLGNIIPLNEAIKKWGPSVLRYWYLTSHYRSPIDFSEEALEQAKSALQRIK
DSMAIIRDVISEGPKFYVKDDDIKVYREILNNLNNFHTAMSNDFDTSTALSYIHEIVRLVFSTLQYSRDFLGAMLAFETL
KQFNEVFGVMDEEFYPTYDKMYKIIDAVVDIRNQLRQMKLYEISDKIREELLKAGVRILDSKDKSTWRFE

Sequences:

>Translated_470_residues
MDFRIRVYNSLGRKLEEFGTVEPNLVKMYVCGPTVYDYVHIGHGRTFVVFDAISRYLRLRGYTVIRVQNITDIDDKIIKK
SQEIGKDWNEIVDYFTKDYLDMLSQLKVKIDIHPRVTQHIREIIDFVQRLIDKGHAYVAPSGSVYFDVDTYPNYGELSNT
KKEEWNQGEEFVKEKKHSYDFALWKAWKPGEPYWESPWGKGRPGWHIECSTMSTRYLGERFDIHGGGADLIFPHHENERA
QTEALIGEKWVTYWVHSAFVTIRKEKMSKSLGNIIPLNEAIKKWGPSVLRYWYLTSHYRSPIDFSEEALEQAKSALQRIK
DSMAIIRDVISEGPKFYVKDDDIKVYREILNNLNNFHTAMSNDFDTSTALSYIHEIVRLVFSTLQYSRDFLGAMLAFETL
KQFNEVFGVMDEEFYPTYDKMYKIIDAVVDIRNQLRQMKLYEISDKIREELLKAGVRILDSKDKSTWRFE
>Mature_470_residues
MDFRIRVYNSLGRKLEEFGTVEPNLVKMYVCGPTVYDYVHIGHGRTFVVFDAISRYLRLRGYTVIRVQNITDIDDKIIKK
SQEIGKDWNEIVDYFTKDYLDMLSQLKVKIDIHPRVTQHIREIIDFVQRLIDKGHAYVAPSGSVYFDVDTYPNYGELSNT
KKEEWNQGEEFVKEKKHSYDFALWKAWKPGEPYWESPWGKGRPGWHIECSTMSTRYLGERFDIHGGGADLIFPHHENERA
QTEALIGEKWVTYWVHSAFVTIRKEKMSKSLGNIIPLNEAIKKWGPSVLRYWYLTSHYRSPIDFSEEALEQAKSALQRIK
DSMAIIRDVISEGPKFYVKDDDIKVYREILNNLNNFHTAMSNDFDTSTALSYIHEIVRLVFSTLQYSRDFLGAMLAFETL
KQFNEVFGVMDEEFYPTYDKMYKIIDAVVDIRNQLRQMKLYEISDKIREELLKAGVRILDSKDKSTWRFE

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=510, Percent_Identity=33.5294117647059, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI10835051, Length=293, Percent_Identity=37.542662116041, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI62240992, Length=293, Percent_Identity=37.542662116041, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI21269877, Length=303, Percent_Identity=36.963696369637, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI303304987, Length=303, Percent_Identity=36.963696369637, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI1786737, Length=476, Percent_Identity=40.3361344537815, Blast_Score=351, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI17509795, Length=323, Percent_Identity=34.3653250773994, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17509797, Length=323, Percent_Identity=34.3653250773994, Blast_Score=194, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6324082, Length=325, Percent_Identity=38.1538461538462, Blast_Score=216, Evalue=6e-57,
Organism=Drosophila melanogaster, GI19922218, Length=505, Percent_Identity=33.2673267326733, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI19922364, Length=318, Percent_Identity=37.1069182389937, Blast_Score=209, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_SULSO (Q97WE6)

Other databases:

- EMBL:   AE006641
- PIR:   C90398
- RefSeq:   NP_343652.1
- ProteinModelPortal:   Q97WE6
- SMR:   Q97WE6
- GeneID:   1453769
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO2280
- NMPDR:   fig|273057.1.peg.2075
- HOGENOM:   HBG327651
- OMA:   PEEMLAG
- PhylomeDB:   Q97WE6
- ProtClustDB:   PRK00260
- BioCyc:   SSOL273057:SSO2280-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_A
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 55327; Mature: 55327

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRIRVYNSLGRKLEEFGTVEPNLVKMYVCGPTVYDYVHIGHGRTFVVFDAISRYLRLR
CCCEEEHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHEEEECCCCEEEHHHHHHHHHHHC
GYTVIRVQNITDIDDKIIKKSQEIGKDWNEIVDYFTKDYLDMLSQLKVKIDIHPRVTQHI
CEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHH
REIIDFVQRLIDKGHAYVAPSGSVYFDVDTYPNYGELSNTKKEEWNQGEEFVKEKKHSYD
HHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCC
FALWKAWKPGEPYWESPWGKGRPGWHIECSTMSTRYLGERFDIHGGGADLIFPHHENERA
CCEEECCCCCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCEECCCCCCEECCCCCCCHH
QTEALIGEKWVTYWVHSAFVTIRKEKMSKSLGNIIPLNEAIKKWGPSVLRYWYLTSHYRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHCCC
PIDFSEEALEQAKSALQRIKDSMAIIRDVISEGPKFYVKDDDIKVYREILNNLNNFHTAM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHH
SNDFDTSTALSYIHEIVRLVFSTLQYSRDFLGAMLAFETLKQFNEVFGVMDEEFYPTYDK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
MYKIIDAVVDIRNQLRQMKLYEISDKIREELLKAGVRILDSKDKSTWRFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDFRIRVYNSLGRKLEEFGTVEPNLVKMYVCGPTVYDYVHIGHGRTFVVFDAISRYLRLR
CCCEEEHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHEEEECCCCEEEHHHHHHHHHHHC
GYTVIRVQNITDIDDKIIKKSQEIGKDWNEIVDYFTKDYLDMLSQLKVKIDIHPRVTQHI
CEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHH
REIIDFVQRLIDKGHAYVAPSGSVYFDVDTYPNYGELSNTKKEEWNQGEEFVKEKKHSYD
HHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCC
FALWKAWKPGEPYWESPWGKGRPGWHIECSTMSTRYLGERFDIHGGGADLIFPHHENERA
CCEEECCCCCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCEECCCCCCEECCCCCCCHH
QTEALIGEKWVTYWVHSAFVTIRKEKMSKSLGNIIPLNEAIKKWGPSVLRYWYLTSHYRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHCCC
PIDFSEEALEQAKSALQRIKDSMAIIRDVISEGPKFYVKDDDIKVYREILNNLNNFHTAM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHH
SNDFDTSTALSYIHEIVRLVFSTLQYSRDFLGAMLAFETLKQFNEVFGVMDEEFYPTYDK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
MYKIIDAVVDIRNQLRQMKLYEISDKIREELLKAGVRILDSKDKSTWRFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726