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The map label for this gene is paaF-4 [C]

Identifier: 15898808

GI number: 15898808

Start: 1831629

End: 1832441

Strand: Reverse

Name: paaF-4 [C]

Synonym: SSO2017

Alternate gene names: 15898808

Gene position: 1832441-1831629 (Counterclockwise)

Preceding gene: 15898809

Following gene: 15898807

Centisome position: 61.24

GC content: 38.62

Gene sequence:

>813_bases
GTGAGACTGATGGGCTTAAGTGAGTTAAATCCAAAGTACTTCAAAATAGAAGTCGAAGACGGAGTAGGAATTATTAAATT
AAATAGATCTCCAGCAAACGCACATAATCTTGAGATGTTGAGGGAATTAGATAATATAATAGTTGAATCGAGATTCGACC
AAAACGTTAAAGCCATACTCATAACTAGTAATATTCCTAGGTTCTTCTCTGCAGGATTTGATATAAATGAAATAAAGGAT
AAGTCCCCCGAGTATATTGGATTATCGAGTCAGTTTAGTAAGGAGGTTATGCTTAGGATGATGTCCACTAAGAAACTAAT
AATTGCTAGCATAAACGGGCATTGTATGGGAGGTGGGTTAGAACTTGCGTTGGCGAGTGATTTAAGATTTGGAGCTAACG
ATGAAAATATAAAATTTGGTATGCCAGAAGTAGCCAATTTGGCCCTAATACCAGGGGAGGGTGGAACACAGTTTTTAGCT
AGGTTAGTGGGTAGATCTAAGGCAATATACTTAATAGTAACCGGAAAGACGCTCTCTCCTAAAGAGGCCTATGAACTCGG
AATATTGGACAGATTAATAGAACCCGAAAAACTATTTGAAGAGTCCTTTGAATTTGCAAGACAAGTGGCTAAAGGTCCAT
CATTAGCTGTAGGATTTACGAAATTAGCGGTAAACGAAGGAATGGATTTACCTTGGTATAATGCGTTTGCCCTAGAAAGG
GAAATGCAAAACCAAGCGTTAGCGTCAGAAGACGCTAAGGAGGGAGCTAGGGCGTTCTTTGAAAAAAGGAGACCAGTATT
TAAAGGGAAATAA

Upstream 100 bases:

>100_bases
TGATGAAATGAGACAGTTATACATTAAGGATATTAAAGAACTAATTACTCCTCTAGGTTTAGAGTTACCAGATATAAATA
GGAATAGAAGGGTATATTAG

Downstream 100 bases:

>100_bases
CGACCGCTGGATGTGAAGTTCCTAAATAACTTTTTCCTAATAAACCCCGTTAAACTATTTCCCACATGTTTTTCCTTAGC
CAATCTTATTTAAATGGAAA

Product: enoyl CoA hydratase (paaF-4)

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MRLMGLSELNPKYFKIEVEDGVGIIKLNRSPANAHNLEMLRELDNIIVESRFDQNVKAILITSNIPRFFSAGFDINEIKD
KSPEYIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGLELALASDLRFGANDENIKFGMPEVANLALIPGEGGTQFLA
RLVGRSKAIYLIVTGKTLSPKEAYELGILDRLIEPEKLFEESFEFARQVAKGPSLAVGFTKLAVNEGMDLPWYNAFALER
EMQNQALASEDAKEGARAFFEKRRPVFKGK

Sequences:

>Translated_270_residues
MRLMGLSELNPKYFKIEVEDGVGIIKLNRSPANAHNLEMLRELDNIIVESRFDQNVKAILITSNIPRFFSAGFDINEIKD
KSPEYIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGLELALASDLRFGANDENIKFGMPEVANLALIPGEGGTQFLA
RLVGRSKAIYLIVTGKTLSPKEAYELGILDRLIEPEKLFEESFEFARQVAKGPSLAVGFTKLAVNEGMDLPWYNAFALER
EMQNQALASEDAKEGARAFFEKRRPVFKGK
>Mature_270_residues
MRLMGLSELNPKYFKIEVEDGVGIIKLNRSPANAHNLEMLRELDNIIVESRFDQNVKAILITSNIPRFFSAGFDINEIKD
KSPEYIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGLELALASDLRFGANDENIKFGMPEVANLALIPGEGGTQFLA
RLVGRSKAIYLIVTGKTLSPKEAYELGILDRLIEPEKLFEESFEFARQVAKGPSLAVGFTKLAVNEGMDLPWYNAFALER
EMQNQALASEDAKEGARAFFEKRRPVFKGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=34.6774193548387, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI20127408, Length=216, Percent_Identity=30.0925925925926, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI4502327, Length=269, Percent_Identity=32.7137546468402, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI68989263, Length=196, Percent_Identity=34.1836734693878, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI31542718, Length=265, Percent_Identity=27.5471698113208, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI62530384, Length=201, Percent_Identity=27.8606965174129, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI295842266, Length=201, Percent_Identity=26.3681592039801, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI150378541, Length=258, Percent_Identity=26.7441860465116, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=27.5109170305677, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=27.5109170305677, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI157694520, Length=187, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI261878539, Length=97, Percent_Identity=42.2680412371134, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594469, Length=188, Percent_Identity=32.9787234042553, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI37594471, Length=188, Percent_Identity=32.9787234042553, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=32.8, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI221142681, Length=251, Percent_Identity=30.6772908366534, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788682, Length=178, Percent_Identity=37.0786516853933, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=33.2046332046332, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=24.9027237354086, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790281, Length=161, Percent_Identity=31.055900621118, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082183, Length=206, Percent_Identity=28.1553398058252, Blast_Score=74, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=277, Percent_Identity=33.5740072202166, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=31.9838056680162, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=214, Percent_Identity=33.1775700934579, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=232, Percent_Identity=31.4655172413793, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17508953, Length=184, Percent_Identity=34.2391304347826, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=185, Percent_Identity=34.0540540540541, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=184, Percent_Identity=34.2391304347826, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=200, Percent_Identity=28.5, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=159, Percent_Identity=27.6729559748428, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=263, Percent_Identity=26.2357414448669, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=184, Percent_Identity=30.9782608695652, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=184, Percent_Identity=30.9782608695652, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=192, Percent_Identity=30.7291666666667, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=31.3953488372093, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI20129971, Length=262, Percent_Identity=33.587786259542, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653477, Length=262, Percent_Identity=33.587786259542, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19921000, Length=187, Percent_Identity=34.7593582887701, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24583077, Length=187, Percent_Identity=34.7593582887701, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24583079, Length=187, Percent_Identity=34.7593582887701, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357171, Length=268, Percent_Identity=25, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921018, Length=224, Percent_Identity=29.0178571428571, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=23.046875, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571729, Length=184, Percent_Identity=33.1521739130435, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28571730, Length=184, Percent_Identity=33.1521739130435, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24583165, Length=213, Percent_Identity=28.6384976525822, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24650670, Length=254, Percent_Identity=24.4094488188976, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30070; Mature: 30070

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLMGLSELNPKYFKIEVEDGVGIIKLNRSPANAHNLEMLRELDNIIVESRFDQNVKAIL
CCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
ITSNIPRFFSAGFDINEIKDKSPEYIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGL
EECCCCHHHHCCCCHHHHCCCCCCEECCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCE
ELALASDLRFGANDENIKFGMPEVANLALIPGEGGTQFLARLVGRSKAIYLIVTGKTLSP
EEEEECCCCCCCCCCCEEECCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCH
KEAYELGILDRLIEPEKLFEESFEFARQVAKGPSLAVGFTKLAVNEGMDLPWYNAFALER
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCHHHHHHHH
EMQNQALASEDAKEGARAFFEKRRPVFKGK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLMGLSELNPKYFKIEVEDGVGIIKLNRSPANAHNLEMLRELDNIIVESRFDQNVKAIL
CCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
ITSNIPRFFSAGFDINEIKDKSPEYIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGL
EECCCCHHHHCCCCHHHHCCCCCCEECCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCE
ELALASDLRFGANDENIKFGMPEVANLALIPGEGGTQFLARLVGRSKAIYLIVTGKTLSP
EEEEECCCCCCCCCCCEEECCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCH
KEAYELGILDRLIEPEKLFEESFEFARQVAKGPSLAVGFTKLAVNEGMDLPWYNAFALER
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCHHHHHHHH
EMQNQALASEDAKEGARAFFEKRRPVFKGK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA