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The map label for this gene is cysA-2 [C]

Identifier: 15898620

GI number: 15898620

Start: 1648800

End: 1649681

Strand: Reverse

Name: cysA-2 [C]

Synonym: SSO1817

Alternate gene names: 15898620

Gene position: 1649681-1648800 (Counterclockwise)

Preceding gene: 15898621

Following gene: 15898619

Centisome position: 55.13

GC content: 38.66

Gene sequence:

>882_bases
ATGAGCCAAACTTTACAACAAGGCGTAATTGTAGATAATAAATGGGTATTAGAGCACTTAAAGGACCCTAATGTAAGAAT
TGTCGAAGTAGACTACGATCCAAACACGGCATATAACGTATGGCATATTCCAGGCTCAGTTCTCATAAGATGGAGGGAGG
ATTTAAGGCATCCTGTGTTAAGGGATTTTATAGAACCTAGTCAATTTGAAAAATTAATGGAAAGTAAAGGGATAAGTAAC
AATACAACAATTGTGCTATACGGTGACTACAATAATTGGTTCGCGGTCTATGCATTTTGGTTATTTAAGGCTTATGGACA
TGAGGATGTAAGAATATTAAATGGCGGAAGAACAAAGTGGGCTAAGGAGAACTTACCAACAGAGCAAGGACCTAAGGAAA
CTCAATACCCCAAGGGTAGCTATAAAGTTAAAAAAGTAGATTGGGGAAATCATAGGGCATTCTTCTGGGAGATCCTTCAA
AAGGTTACTAAGGGAGAGATAGGGAAAACTACATTATTAATTGACGTGAGATCTCCTAAAGAATTTACTGGCGAAATTAG
AGCCCCACCGGAATACGCTGATGAGCAAACTCAAGTAGGTGGACATATACCCGGAGCTATAAGTTTTCCATGGGCTCAAG
CTGTTAATCCAGATACTGGAGAGTTTAAGTCCGTAGAGGAATTAAGAAGATTAGTAGAGAATGTAGGTATAACCTCAGAT
AAAGAAATAATAACGTATTGCAGAATAGGGGAAAGAGCTTCTCATACATGGTTTGTATTAAAGTATTTATTAGGGTATCC
ATCAGTTAGAGTTTATGACGGCTCTTGGGCTGAATGGGGAAATATAGTTGGAGCTCCAGTGAAGAAAGGAGCTGAACCAT
GA

Upstream 100 bases:

>100_bases
AAGGTAAAGGGTAATTTAAAAACTTTTCCTTAAGTGCAAAAGTTGCACAATTCAGTAGAGATATAAATGACAAATCTCAA
TATATACTAGGGATTTAAAG

Downstream 100 bases:

>100_bases
AGGTAATTGAGAAAATAAAATTGGATAGAACTGAGAGTTGCGGATCTAAAAGTCCATTATCAATAATGTTAGATGCAATA
AGGAAAATAAAAGAATGCGA

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSQTLQQGVIVDNKWVLEHLKDPNVRIVEVDYDPNTAYNVWHIPGSVLIRWREDLRHPVLRDFIEPSQFEKLMESKGISN
NTTIVLYGDYNNWFAVYAFWLFKAYGHEDVRILNGGRTKWAKENLPTEQGPKETQYPKGSYKVKKVDWGNHRAFFWEILQ
KVTKGEIGKTTLLIDVRSPKEFTGEIRAPPEYADEQTQVGGHIPGAISFPWAQAVNPDTGEFKSVEELRRLVENVGITSD
KEIITYCRIGERASHTWFVLKYLLGYPSVRVYDGSWAEWGNIVGAPVKKGAEP

Sequences:

>Translated_293_residues
MSQTLQQGVIVDNKWVLEHLKDPNVRIVEVDYDPNTAYNVWHIPGSVLIRWREDLRHPVLRDFIEPSQFEKLMESKGISN
NTTIVLYGDYNNWFAVYAFWLFKAYGHEDVRILNGGRTKWAKENLPTEQGPKETQYPKGSYKVKKVDWGNHRAFFWEILQ
KVTKGEIGKTTLLIDVRSPKEFTGEIRAPPEYADEQTQVGGHIPGAISFPWAQAVNPDTGEFKSVEELRRLVENVGITSD
KEIITYCRIGERASHTWFVLKYLLGYPSVRVYDGSWAEWGNIVGAPVKKGAEP
>Mature_292_residues
SQTLQQGVIVDNKWVLEHLKDPNVRIVEVDYDPNTAYNVWHIPGSVLIRWREDLRHPVLRDFIEPSQFEKLMESKGISNN
TTIVLYGDYNNWFAVYAFWLFKAYGHEDVRILNGGRTKWAKENLPTEQGPKETQYPKGSYKVKKVDWGNHRAFFWEILQK
VTKGEIGKTTLLIDVRSPKEFTGEIRAPPEYADEQTQVGGHIPGAISFPWAQAVNPDTGEFKSVEELRRLVENVGITSDK
EIITYCRIGERASHTWFVLKYLLGYPSVRVYDGSWAEWGNIVGAPVKKGAEP

Specific function: May be a sulfotransferase involved in the formation of thiosulfate [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=285, Percent_Identity=29.8245614035088, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI61835204, Length=286, Percent_Identity=25.5244755244755, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI194473681, Length=286, Percent_Identity=25.5244755244755, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI194473668, Length=286, Percent_Identity=25.5244755244755, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI87082121, Length=291, Percent_Identity=30.9278350515464, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081967, Length=271, Percent_Identity=26.9372693726937, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71997283, Length=304, Percent_Identity=26.3157894736842, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17561888, Length=303, Percent_Identity=26.4026402640264, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115534702, Length=255, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324825, Length=287, Percent_Identity=26.1324041811847, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 33655; Mature: 33524

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTLQQGVIVDNKWVLEHLKDPNVRIVEVDYDPNTAYNVWHIPGSVLIRWREDLRHPVL
CCCCHHCCEEECCHHHHHHCCCCCEEEEEEECCCCCCEEEEECCHHHEEEEHHHHCCHHH
RDFIEPSQFEKLMESKGISNNTTIVLYGDYNNWFAVYAFWLFKAYGHEDVRILNGGRTKW
HHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCH
AKENLPTEQGPKETQYPKGSYKVKKVDWGNHRAFFWEILQKVTKGEIGKTTLLIDVRSPK
HHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCH
EFTGEIRAPPEYADEQTQVGGHIPGAISFPWAQAVNPDTGEFKSVEELRRLVENVGITSD
HHCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCC
KEIITYCRIGERASHTWFVLKYLLGYPSVRVYDGSWAEWGNIVGAPVKKGAEP
HHEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQTLQQGVIVDNKWVLEHLKDPNVRIVEVDYDPNTAYNVWHIPGSVLIRWREDLRHPVL
CCCHHCCEEECCHHHHHHCCCCCEEEEEEECCCCCCEEEEECCHHHEEEEHHHHCCHHH
RDFIEPSQFEKLMESKGISNNTTIVLYGDYNNWFAVYAFWLFKAYGHEDVRILNGGRTKW
HHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCH
AKENLPTEQGPKETQYPKGSYKVKKVDWGNHRAFFWEILQKVTKGEIGKTTLLIDVRSPK
HHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCH
EFTGEIRAPPEYADEQTQVGGHIPGAISFPWAQAVNPDTGEFKSVEELRRLVENVGITSD
HHCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCC
KEIITYCRIGERASHTWFVLKYLLGYPSVRVYDGSWAEWGNIVGAPVKKGAEP
HHEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA