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The map label for this gene is gcvH3

Identifier: 15897967

GI number: 15897967

Start: 956435

End: 956857

Strand: Reverse

Name: gcvH3

Synonym: SSO1105

Alternate gene names: 15897967

Gene position: 956857-956435 (Counterclockwise)

Preceding gene: 15897972

Following gene: 15897966

Centisome position: 31.98

GC content: 35.22

Gene sequence:

>423_bases
ATGAAGATCCTAGGTTTTACATTCCCTGACGATTTACTTTATGAACCTGAAAAACATGTGTGGGTAAGAATAGAGGATAA
TAGTGTAGTTAGTATAGGTGTCACTGATCTAGGACAATATATGGCTGGTAAAATATTCCAAGTTACGGCAAAGCAGAAAG
GTGAGAAAGTAAATGGAAGAAGCGTGCTCTTCTCTATTGAAAGTGCAAAGTGGATAGGTAAGTTTAGGTTGCCAATAGAG
GGTGAAGTCTTTGACGTAAATGAAGAAGTAGTAAAAAACCCTTCAATAATTAATGAAAGACCCTATGATAGTTGGATAGT
TAAGATAAGGGTAGAGGATATGGATATTATAAAAAGAACTTTCAAACCAATTCAAGAGGTATACAAACAATTTGAGGAGG
AGGCAAAAAGAGTTGTTAGATGA

Upstream 100 bases:

>100_bases
GTCTATTTTTAGAATTACACCATCTAACCAAACTGCGAAATTAACCATAATTATGGTTTACTAAATAAAGTTTATAAGCT
TAAAAATATGTCAAATATTT

Downstream 100 bases:

>100_bases
TAAAGAAATACTGTTAAGCGCATTAGATAAGGTTGATAAGTTTTACGTTTACCTTGCCGGAATAAATAGTAGTGAGATAC
TTCTGGTTACTACATTAAAC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MKILGFTFPDDLLYEPEKHVWVRIEDNSVVSIGVTDLGQYMAGKIFQVTAKQKGEKVNGRSVLFSIESAKWIGKFRLPIE
GEVFDVNEEVVKNPSIINERPYDSWIVKIRVEDMDIIKRTFKPIQEVYKQFEEEAKRVVR

Sequences:

>Translated_140_residues
MKILGFTFPDDLLYEPEKHVWVRIEDNSVVSIGVTDLGQYMAGKIFQVTAKQKGEKVNGRSVLFSIESAKWIGKFRLPIE
GEVFDVNEEVVKNPSIINERPYDSWIVKIRVEDMDIIKRTFKPIQEVYKQFEEEAKRVVR
>Mature_140_residues
MKILGFTFPDDLLYEPEKHVWVRIEDNSVVSIGVTDLGQYMAGKIFQVTAKQKGEKVNGRSVLFSIESAKWIGKFRLPIE
GEVFDVNEEVVKNPSIINERPYDSWIVKIRVEDMDIIKRTFKPIQEVYKQFEEEAKRVVR

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=116, Percent_Identity=26.7241379310345, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH3_SULSO (Q97Z34)

Other databases:

- EMBL:   AE006641
- PIR:   C90263
- RefSeq:   NP_342572.1
- ProteinModelPortal:   Q97Z34
- SMR:   Q97Z34
- GeneID:   1454141
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO1105
- NMPDR:   fig|273057.1.peg.995
- HOGENOM:   HBG693789
- OMA:   VESAKWI
- ProtClustDB:   CLSK803006
- BioCyc:   SSOL273057:SSO1105-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR011053
- PANTHER:   PTHR11715

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 16336; Mature: 16336

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILGFTFPDDLLYEPEKHVWVRIEDNSVVSIGVTDLGQYMAGKIFQVTAKQKGEKVNGR
CEEECCCCCHHHCCCCCCCEEEEECCCCEEEEEHHHHHHHHCCCEEEEECCCCCCCCCCC
SVLFSIESAKWIGKFRLPIEGEVFDVNEEVVKNPSIINERPYDSWIVKIRVEDMDIIKRT
EEEEEECCCCEEEEEECCCCCEEEECCHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHH
FKPIQEVYKQFEEEAKRVVR
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILGFTFPDDLLYEPEKHVWVRIEDNSVVSIGVTDLGQYMAGKIFQVTAKQKGEKVNGR
CEEECCCCCHHHCCCCCCCEEEEECCCCEEEEEHHHHHHHHCCCEEEEECCCCCCCCCCC
SVLFSIESAKWIGKFRLPIEGEVFDVNEEVVKNPSIINERPYDSWIVKIRVEDMDIIKRT
EEEEEECCCCEEEEEECCCCCEEEECCHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHH
FKPIQEVYKQFEEEAKRVVR
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726