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The map label for this gene is hyfF [H]

Identifier: 15897892

GI number: 15897892

Start: 882780

End: 884102

Strand: Reverse

Name: hyfF [H]

Synonym: SSO1024

Alternate gene names: 15897892

Gene position: 884102-882780 (Counterclockwise)

Preceding gene: 15897893

Following gene: 15897891

Centisome position: 29.55

GC content: 36.51

Gene sequence:

>1323_bases
ATGAATATTGAACCCATACTCTTACTATTAATTCCTATAATAAGTAACGTAGGCTTTTTCAAACTCAAGCTAATTAAGAC
CCTATCCGTACTATCAGCAGTACTAACATCAATCATCAGCATCTTCCTTTACTTCTTAGCACCAATCAAAAACTCTTTCT
TCTTCATCACTAAGTTCACAACGTTTTTCCTATTGGTGATTGCATCAATTTATCTCCTATCTACCCTTTACTCCATGAAT
TATATAAAACCAAGCAAAATAGTAAGTGAAAGATTATACTACATTCTACTAAATTCCTTCGCATCATCAATGCTTTTTAC
CGTTATTATGAACAATTACGGTCTAATGTGGGTTGGGGTAGAACTCACAACTGTGACCTCAGCTCTTCTAATAATAGCGG
AAGCATCTGAAACCTCACTGGAAGCCACGTGGAGGTATATAATCATAGTATCCGCTGGAGTAACCTTAGCCCTATTCTCA
ATAATTTTCATCTACTATAATTACCATACATTAACTGTAACGGAAATACTTACAAAACCTGAAAACAACATAATAACTAA
ACTTGCGGTAGCTCTAGCTTTAATAGGATTTGGAACAAAAGCAGGAGTTTTCCCCATGTACACGTGGTTACCAGATGCTC
ATAGCGAAGCGCCATCTCCAATAAGCGCATTATTTTCTGGAGTACTACTTCCAGCCGCAACTTACGTGGTGTACATGGTA
TATCAAGTAAATCCATTAACCAATATCTTTGTAATATTCACAACTTTATCGATAATAACCGCATCGATTATCCTAACCTA
TCAATGGCATATAAAGAGAATGTTTGCATACTCAACCATAGAAAACATGAACCTAGCCTTGCTTGGACTTACAATAGGCC
AACCCCTCGGAGCAATAATCCTCCTTCTTGCACACGCATTTGGCAAAGCGGGTGCCTTTTACTCTAGCGGAATTGTATTG
AAAGTCCTTGGAGAAAAGAGAATTGAAAACATAGGCGGTTTACATACAAAACTAAAACTCACCTCAGTATCGCTATTATT
GTCCTCTCTAGCTGTAACTGGTACGCCACCCTTTGCAACTTTCATAGGAGAATTCTTCATATTACAAACATTAATTCAAA
AAGGTTATATTATAGAGTTTATATTAATAGTAATTTCTCTGGCAACAGCTTTCATCTCAATAAACTATAACGTTACCAAA
ATGATATTCACTCAAAGAGAGCTGACAGTCTCAGAAGAACCCAAGCTAATCACGTTCATTTCTCTTGTATCATCTATAAT
TCCACTCGTTCTCGGTATACTTTTACTGGTGATCCTTTCATGA

Upstream 100 bases:

>100_bases
GTGTATTCCTTGATGTATTAGGGCTAGTTATAATTTCCTCAATTTTGACGTTAGAAAAAGCAGAACATTCTAGGTTAGAT
GAACTAAAAGGGTGATCTAA

Downstream 100 bases:

>100_bases
AATACTATAAATGGACTCAAAAAGGCGAGGGAAGGAAAATAGGCAAAATAGGGGATTATTGTCTCTACGAAAAAACGATA
ACAGAAGAGAAATGTGAGGA

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MNIEPILLLLIPIISNVGFFKLKLIKTLSVLSAVLTSIISIFLYFLAPIKNSFFFITKFTTFFLLVIASIYLLSTLYSMN
YIKPSKIVSERLYYILLNSFASSMLFTVIMNNYGLMWVGVELTTVTSALLIIAEASETSLEATWRYIIIVSAGVTLALFS
IIFIYYNYHTLTVTEILTKPENNIITKLAVALALIGFGTKAGVFPMYTWLPDAHSEAPSPISALFSGVLLPAATYVVYMV
YQVNPLTNIFVIFTTLSIITASIILTYQWHIKRMFAYSTIENMNLALLGLTIGQPLGAIILLLAHAFGKAGAFYSSGIVL
KVLGEKRIENIGGLHTKLKLTSVSLLLSSLAVTGTPPFATFIGEFFILQTLIQKGYIIEFILIVISLATAFISINYNVTK
MIFTQRELTVSEEPKLITFISLVSSIIPLVLGILLLVILS

Sequences:

>Translated_440_residues
MNIEPILLLLIPIISNVGFFKLKLIKTLSVLSAVLTSIISIFLYFLAPIKNSFFFITKFTTFFLLVIASIYLLSTLYSMN
YIKPSKIVSERLYYILLNSFASSMLFTVIMNNYGLMWVGVELTTVTSALLIIAEASETSLEATWRYIIIVSAGVTLALFS
IIFIYYNYHTLTVTEILTKPENNIITKLAVALALIGFGTKAGVFPMYTWLPDAHSEAPSPISALFSGVLLPAATYVVYMV
YQVNPLTNIFVIFTTLSIITASIILTYQWHIKRMFAYSTIENMNLALLGLTIGQPLGAIILLLAHAFGKAGAFYSSGIVL
KVLGEKRIENIGGLHTKLKLTSVSLLLSSLAVTGTPPFATFIGEFFILQTLIQKGYIIEFILIVISLATAFISINYNVTK
MIFTQRELTVSEEPKLITFISLVSSIIPLVLGILLLVILS
>Mature_440_residues
MNIEPILLLLIPIISNVGFFKLKLIKTLSVLSAVLTSIISIFLYFLAPIKNSFFFITKFTTFFLLVIASIYLLSTLYSMN
YIKPSKIVSERLYYILLNSFASSMLFTVIMNNYGLMWVGVELTTVTSALLIIAEASETSLEATWRYIIIVSAGVTLALFS
IIFIYYNYHTLTVTEILTKPENNIITKLAVALALIGFGTKAGVFPMYTWLPDAHSEAPSPISALFSGVLLPAATYVVYMV
YQVNPLTNIFVIFTTLSIITASIILTYQWHIKRMFAYSTIENMNLALLGLTIGQPLGAIILLLAHAFGKAGAFYSSGIVL
KVLGEKRIENIGGLHTKLKLTSVSLLLSSLAVTGTPPFATFIGEFFILQTLIQKGYIIEFILIVISLATAFISINYNVTK
MIFTQRELTVSEEPKLITFISLVSSIIPLVLGILLLVILS

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=358, Percent_Identity=27.0949720670391, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788831, Length=350, Percent_Identity=34.5714285714286, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788827, Length=320, Percent_Identity=28.125, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1788613, Length=252, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI2367154, Length=334, Percent_Identity=25.1497005988024, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788614, Length=358, Percent_Identity=27.9329608938547, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788829, Length=334, Percent_Identity=26.6467065868263, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI145693160, Length=288, Percent_Identity=25.6944444444444, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 48654; Mature: 48654

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEPILLLLIPIISNVGFFKLKLIKTLSVLSAVLTSIISIFLYFLAPIKNSFFFITKFT
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
TFFLLVIASIYLLSTLYSMNYIKPSKIVSERLYYILLNSFASSMLFTVIMNNYGLMWVGV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
ELTTVTSALLIIAEASETSLEATWRYIIIVSAGVTLALFSIIFIYYNYHTLTVTEILTKP
EHHHHHHHHHEEEECCCCCHHHEEEEEEEEECCHHHHHHHHHHHHHCCHHEEHHHHHHCC
ENNIITKLAVALALIGFGTKAGVFPMYTWLPDAHSEAPSPISALFSGVLLPAATYVVYMV
CCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YQVNPLTNIFVIFTTLSIITASIILTYQWHIKRMFAYSTIENMNLALLGLTIGQPLGAII
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
LLLAHAFGKAGAFYSSGIVLKVLGEKRIENIGGLHTKLKLTSVSLLLSSLAVTGTPPFAT
HHHHHHHCCCCCCCCCCCEEEECCHHHHHHCCCCHHEEHHHHHHHHHHHHHCCCCCHHHH
FIGEFFILQTLIQKGYIIEFILIVISLATAFISINYNVTKMIFTQRELTVSEEPKLITFI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCEEEEEEEHHCCCCCCCCCHHHHH
SLVSSIIPLVLGILLLVILS
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIEPILLLLIPIISNVGFFKLKLIKTLSVLSAVLTSIISIFLYFLAPIKNSFFFITKFT
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
TFFLLVIASIYLLSTLYSMNYIKPSKIVSERLYYILLNSFASSMLFTVIMNNYGLMWVGV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
ELTTVTSALLIIAEASETSLEATWRYIIIVSAGVTLALFSIIFIYYNYHTLTVTEILTKP
EHHHHHHHHHEEEECCCCCHHHEEEEEEEEECCHHHHHHHHHHHHHCCHHEEHHHHHHCC
ENNIITKLAVALALIGFGTKAGVFPMYTWLPDAHSEAPSPISALFSGVLLPAATYVVYMV
CCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YQVNPLTNIFVIFTTLSIITASIILTYQWHIKRMFAYSTIENMNLALLGLTIGQPLGAII
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
LLLAHAFGKAGAFYSSGIVLKVLGEKRIENIGGLHTKLKLTSVSLLLSSLAVTGTPPFAT
HHHHHHHCCCCCCCCCCCEEEECCHHHHHHCCCCHHEEHHHHHHHHHHHHHCCCCCHHHH
FIGEFFILQTLIQKGYIIEFILIVISLATAFISINYNVTKMIFTQRELTVSEEPKLITFI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCEEEEEEEHHCCCCCCCCCHHHHH
SLVSSIIPLVLGILLLVILS
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]