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The map label for this gene is hycG [C]

Identifier: 15897890

GI number: 15897890

Start: 881006

End: 881611

Strand: Reverse

Name: hycG [C]

Synonym: SSO1022

Alternate gene names: 15897890

Gene position: 881611-881006 (Counterclockwise)

Preceding gene: 15897891

Following gene: 15897889

Centisome position: 29.46

GC content: 39.77

Gene sequence:

>606_bases
GTGAAGAATTGGTGGTTTATTAGAGGTTTAAGAAAAGGAGTAATGACAGAGAAATACCCGAAGGAAATAGCGGAATGGAG
CACTGAAATACAAGGAGAGGGAACTGTAAACTGTCCAACAAACGCCATAAAGGATGGAAAGTGGATAAAGGAAAGATGCG
TATTTTGCAGAAGATGCTATCCCAACTATAGACCAAATCTCAATCCAAGAATATATACAATAAACAAAACACAACCCCTC
TTCAAAAGGTCATTTTACCTTTACCCAATAGACTCCGGCACATGCGGAGGATGTAACATGGAACTGAAGCTGATCTCATC
ACCAGAATACGATATGACAAGATTTAGCATATTCTTCACCAATACTCCAAGACATGCAGACGCATTAGTAGTAATGGGGG
TAATGACAGAGAAAATGAAAGAAGTGTTAAAAAAAGCTTATGAAGCAATGCCAGAACCCAAAGTAGTTATATTATTAGGT
GCTTGCGCTATCTCAGGAGGAATAATAGGCGAAGGAGTACCATTAGAAGCAGTAGTGGAAATCCCCGGTTGTCCACCAAA
TCCCTTTACAATACTCGAAGCCTTGATGAAGGTGAAGGGAAAATGA

Upstream 100 bases:

>100_bases
ACCTATTTTTGAAAAGCGTGATAAACACAATATTTACCGATTTTCAATTCAATTGGGAAAGTTTTGGAATTTGGGTAAGT
GAAATAGGAGTGATGTTAAA

Downstream 100 bases:

>100_bases
ACACGCTACTCTATACATTACCCCCATTTCTAATTTCTATTATATTCTCAATCTTTAATAAAAAAGCGGGATATATCTTG
ACAATGATATCATCAATAAT

Product: formate hydrogenlyase subunit 7 (hycG)

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MKNWWFIRGLRKGVMTEKYPKEIAEWSTEIQGEGTVNCPTNAIKDGKWIKERCVFCRRCYPNYRPNLNPRIYTINKTQPL
FKRSFYLYPIDSGTCGGCNMELKLISSPEYDMTRFSIFFTNTPRHADALVVMGVMTEKMKEVLKKAYEAMPEPKVVILLG
ACAISGGIIGEGVPLEAVVEIPGCPPNPFTILEALMKVKGK

Sequences:

>Translated_201_residues
MKNWWFIRGLRKGVMTEKYPKEIAEWSTEIQGEGTVNCPTNAIKDGKWIKERCVFCRRCYPNYRPNLNPRIYTINKTQPL
FKRSFYLYPIDSGTCGGCNMELKLISSPEYDMTRFSIFFTNTPRHADALVVMGVMTEKMKEVLKKAYEAMPEPKVVILLG
ACAISGGIIGEGVPLEAVVEIPGCPPNPFTILEALMKVKGK
>Mature_201_residues
MKNWWFIRGLRKGVMTEKYPKEIAEWSTEIQGEGTVNCPTNAIKDGKWIKERCVFCRRCYPNYRPNLNPRIYTINKTQPL
FKRSFYLYPIDSGTCGGCNMELKLISSPEYDMTRFSIFFTNTPRHADALVVMGVMTEKMKEVLKKAYEAMPEPKVVILLG
ACAISGGIIGEGVPLEAVVEIPGCPPNPFTILEALMKVKGK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=142, Percent_Identity=33.0985915492958, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=40.1639344262295, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788834, Length=130, Percent_Identity=34.6153846153846, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI1788624, Length=128, Percent_Identity=35.15625, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17509685, Length=114, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24651058, Length=131, Percent_Identity=33.587786259542, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI18859983, Length=114, Percent_Identity=34.2105263157895, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24642371, Length=114, Percent_Identity=34.2105263157895, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 22752; Mature: 22752

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNWWFIRGLRKGVMTEKYPKEIAEWSTEIQGEGTVNCPTNAIKDGKWIKERCVFCRRCY
CCCEEEHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PNYRPNLNPRIYTINKTQPLFKRSFYLYPIDSGTCGGCNMELKLISSPEYDMTRFSIFFT
CCCCCCCCCEEEEECCCCCHHHCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEEE
NTPRHADALVVMGVMTEKMKEVLKKAYEAMPEPKVVILLGACAISGGIIGEGVPLEAVVE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCCCCEEEEEE
IPGCPPNPFTILEALMKVKGK
CCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNWWFIRGLRKGVMTEKYPKEIAEWSTEIQGEGTVNCPTNAIKDGKWIKERCVFCRRCY
CCCEEEHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PNYRPNLNPRIYTINKTQPLFKRSFYLYPIDSGTCGGCNMELKLISSPEYDMTRFSIFFT
CCCCCCCCCEEEEECCCCCHHHCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEEE
NTPRHADALVVMGVMTEKMKEVLKKAYEAMPEPKVVILLGACAISGGIIGEGVPLEAVVE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCCCCEEEEEE
IPGCPPNPFTILEALMKVKGK
CCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]