Click here to switch to the map view.

The map label for this gene is nadB [H]

Identifier: 15897873

GI number: 15897873

Start: 854063

End: 855526

Strand: Reverse

Name: nadB [H]

Synonym: SSO0997

Alternate gene names: 15897873

Gene position: 855526-854063 (Counterclockwise)

Preceding gene: 15897883

Following gene: 15897872

Centisome position: 28.59

GC content: 38.32

Gene sequence:

>1464_bases
TTGGCTAATAAAAAACTTTTTATTTGGTCGAACATATGTTCGAGTATGATCTACATTTTTGGCAGTGGTTTAGCGGGTCT
AAGCGCCGCCATATCCTTGCATAAATCTGGATACAAGGTAACTATTATTTCAAAGAAAATTAATGGGGGCTCAAGTTATT
GGGCGAAGGGTGGTATTGCAGCCGCTGTTGGTAATGATGATTCACCAGAGCTTCACAAGATCGATACTTTGGAAGTGGGT
GATGGGTTATGTGATAGTAAAACTGTCGATTACGTTACAAGAGAGATTCGGTACGTTGTTAGCACAGTTGAAAAATGGGG
TTTCAAATTTGATGATGATTTAAGGTTAGAAGGTGGGCATTCTAAGAGAAGGATATTGCATAAGACTGACGATACTGGAA
GGGAGATAACCAATTTCCTTTTGAACTTAGCTAAGAAAAAGGGAATTAATCTCATTGAAGATAAGTTGCTAGCCTTAAAG
GTTAAAGATGGTAAGGTTGCAGGATTCATAACGGAAAAGGGTGGAAGTTTTGACGCTGAAAAGGTTGTTTTAGCTACAGG
TGGTTATGGCTATTTGTTCAAATTTACTTCGAATCCTAGTACAAACATAGGAGATGGTATAGCCATAGCCTTTAAAGCTG
GTGCTTTGGTTTCAGATACGGAGTTTGTTCAATTTCACCCTACTGTTACAACGTTTGATGGTCAAGCCTATCTGTTGACT
GAAACCCTAAGGGGTGAGGGTGCTATTTTAGTTAATGAGAGGAATGAGAGGTTTGTTTTTAAATATGATAGTAGGGGTGA
ATTGGCTCCAAGGGACGTTTTAAGTAGGGCTATTTATGACCAGTACAAGAAGGGGCATACAGTTTATATCGATCTTTCAC
CTATTGAAGATTTTGATAGGAAGTTCCCCATATTGAGCAATTATGTAAAGAGGTACGGTAAGAGGCTTCAAGTTTTCCCT
GGTGTTCATTATACTATAGGCGGTATTAGAGTTAATACTCGTGGTGAAAGTAATATCAAGGGTTTATATGCAATAGGTGA
GGTTACAGATACTGGTTTACACGGAGCAAATAGGTTGGCCAGTAATTCTCTCGCTGAGGATTTGGTTTATGGTGTAAATC
TTGTAAGATATATTGATAATTGGGAGGGATTAAGTATTGATGACGTTAAGGAGGTTATTGAAGTGAGGCTAAGGAATAGT
AGTAATAGGTTAAGCTTAGAGGAGATTAGGGAGTATAATTGGAATTATTTAGGGATTGTCAGAAATGGCGAGGGACTAGA
TAAGCTAGTTAAGATTTACGAGTCTAATGACACATTTAATGACAACGCTTCGCTTGTAAGCTTATTAAGTGCTAAAGGAG
CGTTGTTAAGAACGGAAAGTAGAGGTGCACATTATAGGGAGGATTACCCTAATAAGAGTTGGGAAGACGGTAAGAGGATA
TATTTCATGGTGAGTAGAAATTGA

Upstream 100 bases:

>100_bases
CATTCTTTTCAGTTTTTAGGTTCTTTATTTGACTGAGTAACTCTTCAACTCTTGTCATTTTCCGAACATATGTTCGTATA
GTAACCTGGTATAAAAACTT

Downstream 100 bases:

>100_bases
TTGATTGGATTTACGTTAAAAGGCTCTTAGATTATTTGGAAGAAGACGTTATGCCGGAGGACATTACTACTAAATTTTTA
GAGGGTATAAGGGCGAGGGG

Product: aspartate oxidase (nadB)

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MANKKLFIWSNICSSMIYIFGSGLAGLSAAISLHKSGYKVTIISKKINGGSSYWAKGGIAAAVGNDDSPELHKIDTLEVG
DGLCDSKTVDYVTREIRYVVSTVEKWGFKFDDDLRLEGGHSKRRILHKTDDTGREITNFLLNLAKKKGINLIEDKLLALK
VKDGKVAGFITEKGGSFDAEKVVLATGGYGYLFKFTSNPSTNIGDGIAIAFKAGALVSDTEFVQFHPTVTTFDGQAYLLT
ETLRGEGAILVNERNERFVFKYDSRGELAPRDVLSRAIYDQYKKGHTVYIDLSPIEDFDRKFPILSNYVKRYGKRLQVFP
GVHYTIGGIRVNTRGESNIKGLYAIGEVTDTGLHGANRLASNSLAEDLVYGVNLVRYIDNWEGLSIDDVKEVIEVRLRNS
SNRLSLEEIREYNWNYLGIVRNGEGLDKLVKIYESNDTFNDNASLVSLLSAKGALLRTESRGAHYREDYPNKSWEDGKRI
YFMVSRN

Sequences:

>Translated_487_residues
MANKKLFIWSNICSSMIYIFGSGLAGLSAAISLHKSGYKVTIISKKINGGSSYWAKGGIAAAVGNDDSPELHKIDTLEVG
DGLCDSKTVDYVTREIRYVVSTVEKWGFKFDDDLRLEGGHSKRRILHKTDDTGREITNFLLNLAKKKGINLIEDKLLALK
VKDGKVAGFITEKGGSFDAEKVVLATGGYGYLFKFTSNPSTNIGDGIAIAFKAGALVSDTEFVQFHPTVTTFDGQAYLLT
ETLRGEGAILVNERNERFVFKYDSRGELAPRDVLSRAIYDQYKKGHTVYIDLSPIEDFDRKFPILSNYVKRYGKRLQVFP
GVHYTIGGIRVNTRGESNIKGLYAIGEVTDTGLHGANRLASNSLAEDLVYGVNLVRYIDNWEGLSIDDVKEVIEVRLRNS
SNRLSLEEIREYNWNYLGIVRNGEGLDKLVKIYESNDTFNDNASLVSLLSAKGALLRTESRGAHYREDYPNKSWEDGKRI
YFMVSRN
>Mature_486_residues
ANKKLFIWSNICSSMIYIFGSGLAGLSAAISLHKSGYKVTIISKKINGGSSYWAKGGIAAAVGNDDSPELHKIDTLEVGD
GLCDSKTVDYVTREIRYVVSTVEKWGFKFDDDLRLEGGHSKRRILHKTDDTGREITNFLLNLAKKKGINLIEDKLLALKV
KDGKVAGFITEKGGSFDAEKVVLATGGYGYLFKFTSNPSTNIGDGIAIAFKAGALVSDTEFVQFHPTVTTFDGQAYLLTE
TLRGEGAILVNERNERFVFKYDSRGELAPRDVLSRAIYDQYKKGHTVYIDLSPIEDFDRKFPILSNYVKRYGKRLQVFPG
VHYTIGGIRVNTRGESNIKGLYAIGEVTDTGLHGANRLASNSLAEDLVYGVNLVRYIDNWEGLSIDDVKEVIEVRLRNSS
NRLSLEEIREYNWNYLGIVRNGEGLDKLVKIYESNDTFNDNASLVSLLSAKGALLRTESRGAHYREDYPNKSWEDGKRIY
FMVSRN

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=523, Percent_Identity=29.6367112810707, Blast_Score=176, Evalue=5e-44,
Organism=Escherichia coli, GI1788928, Length=527, Percent_Identity=36.2428842504744, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI1790597, Length=513, Percent_Identity=31.1890838206628, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1786942, Length=534, Percent_Identity=29.9625468164794, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17505833, Length=525, Percent_Identity=30.8571428571429, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17550100, Length=524, Percent_Identity=30.5343511450382, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71986328, Length=458, Percent_Identity=27.0742358078603, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322701, Length=525, Percent_Identity=32.5714285714286, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322416, Length=543, Percent_Identity=32.0441988950276, Blast_Score=180, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6320788, Length=478, Percent_Identity=29.9163179916318, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6322511, Length=462, Percent_Identity=27.7056277056277, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137288, Length=520, Percent_Identity=29.8076923076923, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24655642, Length=520, Percent_Identity=29.8076923076923, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24655647, Length=520, Percent_Identity=29.8076923076923, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24663005, Length=526, Percent_Identity=27.5665399239544, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 54369; Mature: 54238

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKKLFIWSNICSSMIYIFGSGLAGLSAAISLHKSGYKVTIISKKINGGSSYWAKGGIA
CCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHEEECCCEEEEEEEEECCCCCCEECCCCEE
AAVGNDDSPELHKIDTLEVGDGLCDSKTVDYVTREIRYVVSTVEKWGFKFDDDLRLEGGH
EEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCC
SKRRILHKTDDTGREITNFLLNLAKKKGINLIEDKLLALKVKDGKVAGFITEKGGSFDAE
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHCCCEEEEEECCCCEEEEEEECCCCCCCC
KVVLATGGYGYLFKFTSNPSTNIGDGIAIAFKAGALVSDTEFVQFHPTVTTFDGQAYLLT
EEEEEECCCEEEEEEECCCCCCCCCCEEEEEECCCEECCCEEEEEECEEEEECCCEEEEE
ETLRGEGAILVNERNERFVFKYDSRGELAPRDVLSRAIYDQYKKGHTVYIDLSPIEDFDR
EECCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHC
KFPILSNYVKRYGKRLQVFPGVHYTIGGIRVNTRGESNIKGLYAIGEVTDTGLHGANRLA
CCCHHHHHHHHHCCEEEECCCCEEEEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHH
SNSLAEDLVYGVNLVRYIDNWEGLSIDDVKEVIEVRLRNSSNRLSLEEIREYNWNYLGIV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEE
RNGEGLDKLVKIYESNDTFNDNASLVSLLSAKGALLRTESRGAHYREDYPNKSWEDGKRI
ECCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEE
YFMVSRN
EEEEECC
>Mature Secondary Structure 
ANKKLFIWSNICSSMIYIFGSGLAGLSAAISLHKSGYKVTIISKKINGGSSYWAKGGIA
CCCEEEEHHHHHHHHHHHHCCCHHHHHHHHEEECCCEEEEEEEEECCCCCCEECCCCEE
AAVGNDDSPELHKIDTLEVGDGLCDSKTVDYVTREIRYVVSTVEKWGFKFDDDLRLEGGH
EEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCC
SKRRILHKTDDTGREITNFLLNLAKKKGINLIEDKLLALKVKDGKVAGFITEKGGSFDAE
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHCCCEEEEEECCCCEEEEEEECCCCCCCC
KVVLATGGYGYLFKFTSNPSTNIGDGIAIAFKAGALVSDTEFVQFHPTVTTFDGQAYLLT
EEEEEECCCEEEEEEECCCCCCCCCCEEEEEECCCEECCCEEEEEECEEEEECCCEEEEE
ETLRGEGAILVNERNERFVFKYDSRGELAPRDVLSRAIYDQYKKGHTVYIDLSPIEDFDR
EECCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHC
KFPILSNYVKRYGKRLQVFPGVHYTIGGIRVNTRGESNIKGLYAIGEVTDTGLHGANRLA
CCCHHHHHHHHHCCEEEECCCCEEEEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHH
SNSLAEDLVYGVNLVRYIDNWEGLSIDDVKEVIEVRLRNSSNRLSLEEIREYNWNYLGIV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEE
RNGEGLDKLVKIYESNDTFNDNASLVSLLSAKGALLRTESRGAHYREDYPNKSWEDGKRI
ECCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEE
YFMVSRN
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]