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The map label for this gene is pyK [H]

Identifier: 15897860

GI number: 15897860

Start: 839386

End: 840744

Strand: Reverse

Name: pyK [H]

Synonym: SSO0981

Alternate gene names: 15897860

Gene position: 840744-839386 (Counterclockwise)

Preceding gene: 15897861

Following gene: 15897859

Centisome position: 28.1

GC content: 35.98

Gene sequence:

>1359_bases
ATGAGAAAGACTAAAATAGTTGCTACTTTAGGTCCTTCCTCAGAGGAAAAAGTAAAAGAACTGGCAGAATACGTTGATGT
TTTTAGAATAAATTTTGCACATGGAGACGAAACATCTCATAGGAAGTATTTTGATCTTATTAGAACATATGCACCGGAAT
CTAGTATTATAGTAGATTTGCCAGGGCCTAAGTTGAGACTAGGAGAACTCAAAGAACCAATAGAGGTGAAGAAAGGAGAT
AAGATAGTTTTCTCTCAAAAAGATGGAATTCCAGTTGATGATGAGTTATTTTATTCGGCTGTAAAAGAAAACTCGGATAT
CTTAATTGCAGACGGAACAATACGTGTGAGGGTTAAGTCAAAAGCTAAGGATAGAGTAGAGGGAACCGTAATAGAGGGTG
GAATTTTATTATCGAGAAAAGGAATAAATATTCCTAATGTCAATCTAAAATCTGGGATAACGGACAACGATTTAAAACTT
TTAAAAAGAGCTTTAGATCTGGGAGCAGATTATATAGGACTCTCTTTTGTAATAAGTGAGAATGATGTAAAGAAGGTAAA
GGAATTTGTAGGTGATGAAGCTTGGGTTATCGCGAAGATAGAAAAAAGTGAGGCATTAAAGAACTTAACCAATATCGTTA
ATGAATCGGATGGAATAATGGTAGCCAGAGGCGATTTGGGGGTTGAGACTGGCTTAGAAAATCTGCCTTTAATTCAAAGG
AGAATAGTAAGGACTTCAAGAGTATTTGGCAAACCCGTCATTTTAGCAACTCAAGTATTAACTTCGATGATAAACAGCCC
TATACCTACCAGAGCTGAGATTATAGATATTTCTAACTCGATTATGCAGGGAGTGGACTCTATAATGTTAAGCGATGAAA
CAGCCATAGGCAATTATCCAGTTGAAAGCGTAAGAACTCTTCATAATATCATAAGTAATGTAGAAAAGAGTGTAAAACAT
AGACCAATCGGACCACTAAATAGTGAGAGTGATGCGATAGCTCTAGCTGCTGTAAATGCAAGTAAAGTATCTAAGGCAGA
TGTAATAGTAGTGTATAGTAGATCAGGTAATTCAATATTGCGCGTATCGAGACTGAGACCTGAACGTAACATAATAGGAG
TCTCTCCTGATCCTAGACTAGCTAAAAAGTTTAAGCTTTGTTATGGTGTAATACCCATTAGTATAAACAAAAAGATGCAG
TCCATAGACGAGATAATAGACGTCTCAGCCAAGCTAATGCAGGAAAAAATAAAGGACTTAAAATTTAAAAAAATCGTTAT
AGTAGGAGGGGATCCTAAACAAGAAGCGGGGAAGACTAACTTCGTTATAGTTAAGACACTAGAACAACAAAAGAAATGA

Upstream 100 bases:

>100_bases
ATGCAGTTAGAGAAAGTAATGAAGTCCTAAGGAAGGCATGCGAAAATGGAGTAAAAGAGGCATGCGAAAACATTAAAGAA
ATAGCTAGTAAGATTATGTA

Downstream 100 bases:

>100_bases
TAAAATTATTGCAAATGCTATAATTATAACAGAAAAGATCGTATTCTCAGTCTTTTTTGGTCTTATAGAACCTCTCTTAG
CATTTATCGGAAAATCCTGG

Product: pyruvate kinase (pyK)

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MRKTKIVATLGPSSEEKVKELAEYVDVFRINFAHGDETSHRKYFDLIRTYAPESSIIVDLPGPKLRLGELKEPIEVKKGD
KIVFSQKDGIPVDDELFYSAVKENSDILIADGTIRVRVKSKAKDRVEGTVIEGGILLSRKGINIPNVNLKSGITDNDLKL
LKRALDLGADYIGLSFVISENDVKKVKEFVGDEAWVIAKIEKSEALKNLTNIVNESDGIMVARGDLGVETGLENLPLIQR
RIVRTSRVFGKPVILATQVLTSMINSPIPTRAEIIDISNSIMQGVDSIMLSDETAIGNYPVESVRTLHNIISNVEKSVKH
RPIGPLNSESDAIALAAVNASKVSKADVIVVYSRSGNSILRVSRLRPERNIIGVSPDPRLAKKFKLCYGVIPISINKKMQ
SIDEIIDVSAKLMQEKIKDLKFKKIVIVGGDPKQEAGKTNFVIVKTLEQQKK

Sequences:

>Translated_452_residues
MRKTKIVATLGPSSEEKVKELAEYVDVFRINFAHGDETSHRKYFDLIRTYAPESSIIVDLPGPKLRLGELKEPIEVKKGD
KIVFSQKDGIPVDDELFYSAVKENSDILIADGTIRVRVKSKAKDRVEGTVIEGGILLSRKGINIPNVNLKSGITDNDLKL
LKRALDLGADYIGLSFVISENDVKKVKEFVGDEAWVIAKIEKSEALKNLTNIVNESDGIMVARGDLGVETGLENLPLIQR
RIVRTSRVFGKPVILATQVLTSMINSPIPTRAEIIDISNSIMQGVDSIMLSDETAIGNYPVESVRTLHNIISNVEKSVKH
RPIGPLNSESDAIALAAVNASKVSKADVIVVYSRSGNSILRVSRLRPERNIIGVSPDPRLAKKFKLCYGVIPISINKKMQ
SIDEIIDVSAKLMQEKIKDLKFKKIVIVGGDPKQEAGKTNFVIVKTLEQQKK
>Mature_452_residues
MRKTKIVATLGPSSEEKVKELAEYVDVFRINFAHGDETSHRKYFDLIRTYAPESSIIVDLPGPKLRLGELKEPIEVKKGD
KIVFSQKDGIPVDDELFYSAVKENSDILIADGTIRVRVKSKAKDRVEGTVIEGGILLSRKGINIPNVNLKSGITDNDLKL
LKRALDLGADYIGLSFVISENDVKKVKEFVGDEAWVIAKIEKSEALKNLTNIVNESDGIMVARGDLGVETGLENLPLIQR
RIVRTSRVFGKPVILATQVLTSMINSPIPTRAEIIDISNSIMQGVDSIMLSDETAIGNYPVESVRTLHNIISNVEKSVKH
RPIGPLNSESDAIALAAVNASKVSKADVIVVYSRSGNSILRVSRLRPERNIIGVSPDPRLAKKFKLCYGVIPISINKKMQ
SIDEIIDVSAKLMQEKIKDLKFKKIVIVGGDPKQEAGKTNFVIVKTLEQQKK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=484, Percent_Identity=33.8842975206612, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI32967597, Length=440, Percent_Identity=33.8636363636364, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI10835121, Length=440, Percent_Identity=33.8636363636364, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI33286422, Length=484, Percent_Identity=33.6776859504132, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI33286420, Length=484, Percent_Identity=33.6776859504132, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=32.2916666666667, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=32.2916666666667, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=29.1139240506329, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=29.1139240506329, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI310128738, Length=211, Percent_Identity=27.9620853080569, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=31.0126582278481, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=31.7427385892116, Blast_Score=205, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=33.2644628099174, Blast_Score=227, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17506829, Length=488, Percent_Identity=31.3524590163934, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI71984406, Length=488, Percent_Identity=31.3524590163934, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI71984413, Length=488, Percent_Identity=31.3524590163934, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17506831, Length=488, Percent_Identity=31.3524590163934, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6319279, Length=444, Percent_Identity=31.0810810810811, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324923, Length=427, Percent_Identity=28.8056206088993, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24648966, Length=404, Percent_Identity=35.3960396039604, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24648964, Length=491, Percent_Identity=32.7902240325866, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI28571814, Length=491, Percent_Identity=32.7902240325866, Blast_Score=208, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24581235, Length=470, Percent_Identity=28.936170212766, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24646914, Length=257, Percent_Identity=38.1322957198444, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 49885; Mature: 49885

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKIVATLGPSSEEKVKELAEYVDVFRINFAHGDETSHRKYFDLIRTYAPESSIIVDL
CCCEEEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEC
PGPKLRLGELKEPIEVKKGDKIVFSQKDGIPVDDELFYSAVKENSDILIADGTIRVRVKS
CCCCEECCCCCCCCEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCEEEEEECC
KAKDRVEGTVIEGGILLSRKGINIPNVNLKSGITDNDLKLLKRALDLGADYIGLSFVISE
CCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECC
NDVKKVKEFVGDEAWVIAKIEKSEALKNLTNIVNESDGIMVARGDLGVETGLENLPLIQR
CHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCHHHH
RIVRTSRVFGKPVILATQVLTSMINSPIPTRAEIIDISNSIMQGVDSIMLSDETAIGNYP
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCC
VESVRTLHNIISNVEKSVKHRPIGPLNSESDAIALAAVNASKVSKADVIVVYSRSGNSIL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCEE
RVSRLRPERNIIGVSPDPRLAKKFKLCYGVIPISINKKMQSIDEIIDVSAKLMQEKIKDL
EEEECCCCCCEEECCCCHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCC
KFKKIVIVGGDPKQEAGKTNFVIVKTLEQQKK
CEEEEEEECCCCHHHCCCCCEEEEEECHHCCC
>Mature Secondary Structure
MRKTKIVATLGPSSEEKVKELAEYVDVFRINFAHGDETSHRKYFDLIRTYAPESSIIVDL
CCCEEEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEC
PGPKLRLGELKEPIEVKKGDKIVFSQKDGIPVDDELFYSAVKENSDILIADGTIRVRVKS
CCCCEECCCCCCCCEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCEEEEEECC
KAKDRVEGTVIEGGILLSRKGINIPNVNLKSGITDNDLKLLKRALDLGADYIGLSFVISE
CCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECC
NDVKKVKEFVGDEAWVIAKIEKSEALKNLTNIVNESDGIMVARGDLGVETGLENLPLIQR
CHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCHHHH
RIVRTSRVFGKPVILATQVLTSMINSPIPTRAEIIDISNSIMQGVDSIMLSDETAIGNYP
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCC
VESVRTLHNIISNVEKSVKHRPIGPLNSESDAIALAAVNASKVSKADVIVVYSRSGNSIL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCEE
RVSRLRPERNIIGVSPDPRLAKKFKLCYGVIPISINKKMQSIDEIIDVSAKLMQEKIKDL
EEEECCCCCCEEECCCCHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCC
KFKKIVIVGGDPKQEAGKTNFVIVKTLEQQKK
CEEEEEEECCCCHHHCCCCCEEEEEECHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7592429 [H]