Click here to switch to the map view.

The map label for this gene is TFB-2

Identifier: 15897826

GI number: 15897826

Start: 802675

End: 803556

Strand: Reverse

Name: TFB-2

Synonym: SSO0946

Alternate gene names: 15897826

Gene position: 803556-802675 (Counterclockwise)

Preceding gene: 15897830

Following gene: 15897825

Centisome position: 26.85

GC content: 34.13

Gene sequence:

>882_bases
ATGAAATGCCCATATTGTAAAACAGATAATGCAATAACTTATGATGTGGAAAAAGGAATGTACGTTTGTACAAATTGTGC
CAGCGTAATAGAGGATAGTGCAGTAGATCCTGGTCCAGATTGGAGAGCCTATAACGCAAAAGACAGAAATGAAAAAGAAA
GAGTAGGTAGTCCAAGCACTCCAAAAGTACATGACTGGGGATTTCATACTATAATAGGGTACGGTAGGGCTAAAGATAGA
CTAAAAACATTGAAAATGCAAAGAATGCAAAATAAAATACGTGTATCGCCTAAGGATAAAAAGCTAGTCACCTTACTTTC
AATTTTAAATGATGAGAGCTCAAAGTTAGAATTGCCAGAACATGTCAAAGAGACTGCATCCTTAATAATAAGGAAAATGG
TAGAAACTGGACTAACTAAAAGGATTGATCAGTATACGTTAATAGTAGCCGCCTTATACTATTCTTGCCAAGTAAACAAC
ATACCGAGACATCTCCAAGAATTTAAAGTTAGATATTCCATATCTTCTAGTGAATTTTGGAGCGCGTTGAAAAGAGTCCA
ATATGTTGCAAATTCAATACCAGGCTTTCGTCCTAAAATAAAACCAGCAGAATATATACCGAAGATATTATATAAACTAA
ATTTACCTCCAATTATAGGAACAAAGGCATCTGAATTAGTAGACCTTATGCATAAGCAAGGGTTAACAAGTGGTAAAGGA
TACTTATCTTTGAGCGCAGCTTCCGTTTACTTAATATCAGCATTAATGGATATAAAAAAGACGCAGAAAGAAGTTGCAGA
TTCATTAGATATTACCGAAGTAACTATAAGGAATAGATATAAAGATATCGTGGATAATTTTGACATTGTAGTTACATTAT
GA

Upstream 100 bases:

>100_bases
ACGTAAAAGAACCATATGCTCTAGCTATGTTAGATAAAGATTTAGAAATTTCGGAAACTGAAAAAATATTTGTAAACCAC
AGCGAGAGGAGGTCAAGAAA

Downstream 100 bases:

>100_bases
TTTTTGCCGAATTAAATAAAATTGTTATAAGAAGAATTTATTTGTTTTAAATGTTAATTATAAGTAAAAGTATTAAAAAG
TGATCAATCATCAGTAAGGA

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB 2

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKCPYCKTDNAITYDVEKGMYVCTNCASVIEDSAVDPGPDWRAYNAKDRNEKERVGSPSTPKVHDWGFHTIIGYGRAKDR
LKTLKMQRMQNKIRVSPKDKKLVTLLSILNDESSKLELPEHVKETASLIIRKMVETGLTKRIDQYTLIVAALYYSCQVNN
IPRHLQEFKVRYSISSSEFWSALKRVQYVANSIPGFRPKIKPAEYIPKILYKLNLPPIIGTKASELVDLMHKQGLTSGKG
YLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIVVTL

Sequences:

>Translated_293_residues
MKCPYCKTDNAITYDVEKGMYVCTNCASVIEDSAVDPGPDWRAYNAKDRNEKERVGSPSTPKVHDWGFHTIIGYGRAKDR
LKTLKMQRMQNKIRVSPKDKKLVTLLSILNDESSKLELPEHVKETASLIIRKMVETGLTKRIDQYTLIVAALYYSCQVNN
IPRHLQEFKVRYSISSSEFWSALKRVQYVANSIPGFRPKIKPAEYIPKILYKLNLPPIIGTKASELVDLMHKQGLTSGKG
YLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIVVTL
>Mature_293_residues
MKCPYCKTDNAITYDVEKGMYVCTNCASVIEDSAVDPGPDWRAYNAKDRNEKERVGSPSTPKVHDWGFHTIIGYGRAKDR
LKTLKMQRMQNKIRVSPKDKKLVTLLSILNDESSKLELPEHVKETASLIIRKMVETGLTKRIDQYTLIVAALYYSCQVNN
IPRHLQEFKVRYSISSSEFWSALKRVQYVANSIPGFRPKIKPAEYIPKILYKLNLPPIIGTKASELVDLMHKQGLTSGKG
YLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIVVTL

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger

Homologues:

Organism=Homo sapiens, GI4504193, Length=280, Percent_Identity=23.5714285714286, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17564892, Length=286, Percent_Identity=25.1748251748252, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325343, Length=303, Percent_Identity=24.7524752475248, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17136758, Length=281, Percent_Identity=24.1992882562278, Blast_Score=114, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B2_SULSO (P58110)

Other databases:

- EMBL:   AE006641
- PIR:   F90245
- RefSeq:   NP_342431.1
- ProteinModelPortal:   P58110
- GeneID:   1455188
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0946
- NMPDR:   fig|273057.1.peg.854
- HOGENOM:   HBG646536
- OMA:   NGLTSGK
- PhylomeDB:   P58110
- ProtClustDB:   CLSK802933
- BioCyc:   SSOL273057:SSO0946-MONOMER
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00685
- SMART:   SM00385

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 33284; Mature: 33284

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00782 TFIIB; PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCPYCKTDNAITYDVEKGMYVCTNCASVIEDSAVDPGPDWRAYNAKDRNEKERVGSPST
CCCCCCCCCCCEEEECCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCC
PKVHDWGFHTIIGYGRAKDRLKTLKMQRMQNKIRVSPKDKKLVTLLSILNDESSKLELPE
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHH
HVKETASLIIRKMVETGLTKRIDQYTLIVAALYYSCQVNNIPRHLQEFKVRYSISSSEFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
SALKRVQYVANSIPGFRPKIKPAEYIPKILYKLNLPPIIGTKASELVDLMHKQGLTSGKG
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
YLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIVVTL
EEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MKCPYCKTDNAITYDVEKGMYVCTNCASVIEDSAVDPGPDWRAYNAKDRNEKERVGSPST
CCCCCCCCCCCEEEECCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCC
PKVHDWGFHTIIGYGRAKDRLKTLKMQRMQNKIRVSPKDKKLVTLLSILNDESSKLELPE
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHH
HVKETASLIIRKMVETGLTKRIDQYTLIVAALYYSCQVNNIPRHLQEFKVRYSISSSEFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
SALKRVQYVANSIPGFRPKIKPAEYIPKILYKLNLPPIIGTKASELVDLMHKQGLTSGKG
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
YLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIVVTL
EEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726