Click here to switch to the map view.

The map label for this gene is ansA [H]

Identifier: 15897818

GI number: 15897818

Start: 796129

End: 797463

Strand: Reverse

Name: ansA [H]

Synonym: SSO0938

Alternate gene names: 15897818

Gene position: 797463-796129 (Counterclockwise)

Preceding gene: 15897819

Following gene: 15897817

Centisome position: 26.65

GC content: 34.98

Gene sequence:

>1335_bases
GTGCAAGAAAATTATAAAGGAAAGGCCTATGATATTTTGAAAAATCTAAATATAGAGGAAGGAGATCTTATAGAAATTAA
GAAAGGAGACTTAAGAATAAGAGGTATATTGTTACCAAGTTACTCTAAAGATGATAGAATATTCGTAATAAAATTAGATA
ATGGTTATAATATAGGAATATCTATAGATAATATAACTGAGATAAAACCAATCGAAAAAAAATCAAGTAAAGATCGGGAG
AGTGAAAGAAGAGAAGTTCATAATGGTGCTAAGAGCGAAATAAAGATAATTAGCACAGGGGGCACAATAGTAAGTAGGGT
TGAATATGAAACTGGTGCAGTCAGGCCTGCATTAACCACTGAAGAAATAATACAATTTTTACCAGAAATAAACGAAATAG
CAAAAGTGGATGCTGAGGTACTTTTCTCTATATTAAGTGAAAACATGAAACCCGAATTCTGGGTTAAGATAGCTGAATCA
GTAAAGAAGGCCTTTGATGAAGGAAACACTGGTATAGTTATTGCCCATGGTACGGACACTATGGCATATACAGCCTCAGC
CTTAGCCTTCTCTCTAAGATCGTTACAAGGACCTGTAGTACTAGTGGGGTCACAGAGAAGTAGTGATAGACCTAGTAGCG
ATTCAGCAATAAACCTCTTATCTGCAGTTATCACGGCTAAATATGCACCGTTTGGAGAAGTAGTAGTAAATATGCATGCC
GAATCCTCAGATACTTACGCATTAGTACATAGAGGAGTAAAGGTTAGAAAAATGCACAGCAGTAGAAGAGATGCTTTTCA
GTCAGTGAACGATAAACCTTTAGCTAAAGTTCTTTGGAAGGAAAGGAAACTAGTAATGCTTAACGAGAAGTATATAAGCA
AAAAAGACGAAACGTTGCTTGACGCTAAATTCGATAATAGAGTATTTTTGTTATACTATTATCCAGGATTAGATAGAGAC
TTCTTAGAACAAATGTTAACTAATACTAAAATAAGAGGTATAATCATAGCTGGTACTGGATTAGGACATACTTCTTCAGA
TCACATAGAATTATTCAGAAAGGCTACTAAAGATGGTATATTTATAGGAATGACAACACAGTGTCTATTTGGAAGAGTTA
ATATGAATGTATATACTACTGGAAGACAGTTACTCGATGCTGGTGTCACCCCATTGGAAGATATGCTACCAGAGGTAGCA
TTAGTAAAACTCATGTGGGTACTTGCACATGAGCAAGATTTAGAAAAAATACAAAATTTAATGATAACAAATCTCGTGGG
AGAAATAAATCCTCGTCACACTTTAGATCTCTTCCCAAGGTGGTCATATGAGTGA

Upstream 100 bases:

>100_bases
AGTAAGAAATAGAAAGGAATATGAAAAGAGAGTAGTAAAAGCCAGACAACAAGCTCCTGCTAGATAATAAACTATTTTTG
CTATTACCTAATCTTTTTCT

Downstream 100 bases:

>100_bases
GTTGAATTATGAAGAATTGGGGTTAAAAGTAGGATTAGAGATTCATCAACAGCTTAACACTCCTCATAAATTATTCTGCA
ACTGCAGTACTAATTTGGAA

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MQENYKGKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDDRIFVIKLDNGYNIGISIDNITEIKPIEKKSSKDRE
SERREVHNGAKSEIKIISTGGTIVSRVEYETGAVRPALTTEEIIQFLPEINEIAKVDAEVLFSILSENMKPEFWVKIAES
VKKAFDEGNTGIVIAHGTDTMAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVITAKYAPFGEVVVNMHA
ESSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLNEKYISKKDETLLDAKFDNRVFLLYYYPGLDRD
FLEQMLTNTKIRGIIIAGTGLGHTSSDHIELFRKATKDGIFIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEVA
LVKLMWVLAHEQDLEKIQNLMITNLVGEINPRHTLDLFPRWSYE

Sequences:

>Translated_444_residues
MQENYKGKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDDRIFVIKLDNGYNIGISIDNITEIKPIEKKSSKDRE
SERREVHNGAKSEIKIISTGGTIVSRVEYETGAVRPALTTEEIIQFLPEINEIAKVDAEVLFSILSENMKPEFWVKIAES
VKKAFDEGNTGIVIAHGTDTMAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVITAKYAPFGEVVVNMHA
ESSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLNEKYISKKDETLLDAKFDNRVFLLYYYPGLDRD
FLEQMLTNTKIRGIIIAGTGLGHTSSDHIELFRKATKDGIFIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEVA
LVKLMWVLAHEQDLEKIQNLMITNLVGEINPRHTLDLFPRWSYE
>Mature_444_residues
MQENYKGKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDDRIFVIKLDNGYNIGISIDNITEIKPIEKKSSKDRE
SERREVHNGAKSEIKIISTGGTIVSRVEYETGAVRPALTTEEIIQFLPEINEIAKVDAEVLFSILSENMKPEFWVKIAES
VKKAFDEGNTGIVIAHGTDTMAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVITAKYAPFGEVVVNMHA
ESSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLNEKYISKKDETLLDAKFDNRVFLLYYYPGLDRD
FLEQMLTNTKIRGIIIAGTGLGHTSSDHIELFRKATKDGIFIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEVA
LVKLMWVLAHEQDLEKIQNLMITNLVGEINPRHTLDLFPRWSYE

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=373, Percent_Identity=29.4906166219839, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788065, Length=346, Percent_Identity=32.9479768786127, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1789327, Length=308, Percent_Identity=28.2467532467532, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193207374, Length=334, Percent_Identity=32.0359281437126, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI193207372, Length=334, Percent_Identity=32.0359281437126, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17558280, Length=334, Percent_Identity=32.0359281437126, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17558278, Length=334, Percent_Identity=32.0359281437126, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320527, Length=138, Percent_Identity=34.0579710144928, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323189, Length=279, Percent_Identity=25.089605734767, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323187, Length=279, Percent_Identity=25.089605734767, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323186, Length=279, Percent_Identity=25.089605734767, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323184, Length=279, Percent_Identity=25.089605734767, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24639609, Length=375, Percent_Identity=30.1333333333333, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24645530, Length=283, Percent_Identity=32.1554770318021, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: 3.5.1.1

Molecular weight: Translated: 50017; Mature: 50017

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQENYKGKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDDRIFVIKLDNGYNIGI
CCCCCCCHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCCEEEEEEECCCEEEEE
SIDNITEIKPIEKKSSKDRESERREVHNGAKSEIKIISTGGTIVSRVEYETGAVRPALTT
EECCCCCCCCCHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCH
EEIIQFLPEINEIAKVDAEVLFSILSENMKPEFWVKIAESVKKAFDEGNTGIVIAHGTDT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
MAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVITAKYAPFGEVVVNMHA
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEC
ESSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLNEKYISKKDETLL
CCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECHHHHCCCCCEEE
DAKFDNRVFLLYYYPGLDRDFLEQMLTNTKIRGIIIAGTGLGHTSSDHIELFRKATKDGI
EEECCCEEEEEEEECCCCHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCE
FIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEVALVKLMWVLAHEQDLEKIQNL
EEEEEHHHHEEEECEEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MITNLVGEINPRHTLDLFPRWSYE
HHHHHHHCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MQENYKGKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDDRIFVIKLDNGYNIGI
CCCCCCCHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCCEEEEEEECCCEEEEE
SIDNITEIKPIEKKSSKDRESERREVHNGAKSEIKIISTGGTIVSRVEYETGAVRPALTT
EECCCCCCCCCHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCH
EEIIQFLPEINEIAKVDAEVLFSILSENMKPEFWVKIAESVKKAFDEGNTGIVIAHGTDT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
MAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVITAKYAPFGEVVVNMHA
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEC
ESSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLNEKYISKKDETLL
CCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECHHHHCCCCCEEE
DAKFDNRVFLLYYYPGLDRDFLEQMLTNTKIRGIIIAGTGLGHTSSDHIELFRKATKDGI
EEECCCEEEEEEEECCCCHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCE
FIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEVALVKLMWVLAHEQDLEKIQNL
EEEEEHHHHEEEECEEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MITNLVGEINPRHTLDLFPRWSYE
HHHHHHHCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA