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The map label for this gene is yiaO [H]

Identifier: 15889993

GI number: 15889993

Start: 2741202

End: 2742209

Strand: Direct

Name: yiaO [H]

Synonym: Atu2744

Alternate gene names: 15889993

Gene position: 2741202-2742209 (Clockwise)

Preceding gene: 15889992

Following gene: 159185359

Centisome position: 96.47

GC content: 58.53

Gene sequence:

>1008_bases
ATGAGAAAGCTTCTTCTGACCACCACGGCAATCGCCTTCGCCGTTGGCGCCGCTGCCCCGGCAATGGCCGAATTCAACAG
CCGCAACATCCGTGTTTCGAACGGCATCAACCAGGACCATCCTGTCGGCAACGGCATCAAGGCCATGCAGGCCTGCCTCG
ACGACAAGTCGGGCGGCAAGCTGAAGCTGACAGCATTCTGGGGTGGCGCTCTCGGCGGCGACCTTCAGGCGACGCAGGCC
CTGCGCTCCGGCGTTCAGGAAGCCGTCGTCACATCGTCCTCGCCGCTCGTCGGTCTCATTCCGTCGCTCGGCGTCTTCGA
CCTGCCGTTCCTCTTTGCCAATGACAAAGAAGCCGATGCCGTGATGGACGGCGCCTTTGGCGACATGATGAACAAGAAGC
TGGAAGAACAGGGCCTCGTGAACCTGGCCTACTGGGAGAACGGCTTCCGCAACCTTTCCAACTCCAAGCACGCCGTGAAC
AAGTGGGAAGATTTCTCGGGCCTCAAGGTCCGCGTGATGCAGAACAACATCTTCCTCGACACCTTCCAGAACCTCGGCGC
CAATGCAACGCCGATGGCTTTTGGTGAAGTGTTCTCAGCGCTTGAAACCAATGCGATCGACGCGCAGGAAAACCCCTATG
TGACGATCGACACTTCCAAGTTCTACGAGGTGCAGAAATACGTCACCGAGACGAACCACGCCTACACGCCGTTCCTCTTC
CTCTTCTCCAAGCCGATCTTCGACAGCTACACGCCCGAAGAACAGACGGCCCTGCGTGAATGCGCAGTCGTCGGTCGCGA
TGAAGAGCGCAAGGTCATCCGCGAACTGAACCAGAAGTCGCTGGAAAAGATCAAGGCTGCCGGCCTCGAGGTCAACACGC
TGTCTCCGGAAGAGCAGACCCGTATCCGCGAAAAGTCGATGGTCGTTTACGAAAAGCACAAGGCTGAAATCGGCGCTGAT
GTCGTTGACGCCGTTCTGGCCGACCTGAAGAAAATCCGCGGCCAGTAA

Upstream 100 bases:

>100_bases
AACCATCGTTCTGGCGCTGCTGGTGATTTTCCCCGACATCGTCATGGTTCCGTTGCAGTACCTTCGTTAATTTCTCGAAC
ACAATCCAAGGGAGGATATT

Downstream 100 bases:

>100_bases
GCCTATCTGTATTGAATAAAAAACACCCGGAAGTTTGCTTCCGGGTGTTTTCGTTTGCGGAAAGAAAAGGCTGCCGAAGC
AGCCTTTCCAGAAGATCAGG

Product: C4-dicarboxylate binding protein

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MRKLLLTTTAIAFAVGAAAPAMAEFNSRNIRVSNGINQDHPVGNGIKAMQACLDDKSGGKLKLTAFWGGALGGDLQATQA
LRSGVQEAVVTSSSPLVGLIPSLGVFDLPFLFANDKEADAVMDGAFGDMMNKKLEEQGLVNLAYWENGFRNLSNSKHAVN
KWEDFSGLKVRVMQNNIFLDTFQNLGANATPMAFGEVFSALETNAIDAQENPYVTIDTSKFYEVQKYVTETNHAYTPFLF
LFSKPIFDSYTPEEQTALRECAVVGRDEERKVIRELNQKSLEKIKAAGLEVNTLSPEEQTRIREKSMVVYEKHKAEIGAD
VVDAVLADLKKIRGQ

Sequences:

>Translated_335_residues
MRKLLLTTTAIAFAVGAAAPAMAEFNSRNIRVSNGINQDHPVGNGIKAMQACLDDKSGGKLKLTAFWGGALGGDLQATQA
LRSGVQEAVVTSSSPLVGLIPSLGVFDLPFLFANDKEADAVMDGAFGDMMNKKLEEQGLVNLAYWENGFRNLSNSKHAVN
KWEDFSGLKVRVMQNNIFLDTFQNLGANATPMAFGEVFSALETNAIDAQENPYVTIDTSKFYEVQKYVTETNHAYTPFLF
LFSKPIFDSYTPEEQTALRECAVVGRDEERKVIRELNQKSLEKIKAAGLEVNTLSPEEQTRIREKSMVVYEKHKAEIGAD
VVDAVLADLKKIRGQ
>Mature_335_residues
MRKLLLTTTAIAFAVGAAAPAMAEFNSRNIRVSNGINQDHPVGNGIKAMQACLDDKSGGKLKLTAFWGGALGGDLQATQA
LRSGVQEAVVTSSSPLVGLIPSLGVFDLPFLFANDKEADAVMDGAFGDMMNKKLEEQGLVNLAYWENGFRNLSNSKHAVN
KWEDFSGLKVRVMQNNIFLDTFQNLGANATPMAFGEVFSALETNAIDAQENPYVTIDTSKFYEVQKYVTETNHAYTPFLF
LFSKPIFDSYTPEEQTALRECAVVGRDEERKVIRELNQKSLEKIKAAGLEVNTLSPEEQTRIREKSMVVYEKHKAEIGAD
VVDAVLADLKKIRGQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=289, Percent_Identity=35.6401384083045, Blast_Score=188, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36825; Mature: 36825

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLLTTTAIAFAVGAAAPAMAEFNSRNIRVSNGINQDHPVGNGIKAMQACLDDKSGGK
CCCHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCE
LKLTAFWGGALGGDLQATQALRSGVQEAVVTSSSPLVGLIPSLGVFDLPFLFANDKEADA
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHCCEEEECCCCHHH
VMDGAFGDMMNKKLEEQGLVNLAYWENGFRNLSNSKHAVNKWEDFSGLKVRVMQNNIFLD
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEECCEEEE
TFQNLGANATPMAFGEVFSALETNAIDAQENPYVTIDTSKFYEVQKYVTETNHAYTPFLF
HHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCHHHH
LFSKPIFDSYTPEEQTALRECAVVGRDEERKVIRELNQKSLEKIKAAGLEVNTLSPEEQT
HHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHH
RIREKSMVVYEKHKAEIGADVVDAVLADLKKIRGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKLLLTTTAIAFAVGAAAPAMAEFNSRNIRVSNGINQDHPVGNGIKAMQACLDDKSGGK
CCCHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCE
LKLTAFWGGALGGDLQATQALRSGVQEAVVTSSSPLVGLIPSLGVFDLPFLFANDKEADA
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHCCEEEECCCCHHH
VMDGAFGDMMNKKLEEQGLVNLAYWENGFRNLSNSKHAVNKWEDFSGLKVRVMQNNIFLD
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEECCEEEE
TFQNLGANATPMAFGEVFSALETNAIDAQENPYVTIDTSKFYEVQKYVTETNHAYTPFLF
HHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCHHHH
LFSKPIFDSYTPEEQTALRECAVVGRDEERKVIRELNQKSLEKIKAAGLEVNTLSPEEQT
HHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHH
RIREKSMVVYEKHKAEIGADVVDAVLADLKKIRGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]