Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 15889955

GI number: 15889955

Start: 2685039

End: 2685737

Strand: Direct

Name: trmD

Synonym: Atu2701

Alternate gene names: 15889955

Gene position: 2685039-2685737 (Clockwise)

Preceding gene: 159185334

Following gene: 15889957

Centisome position: 94.49

GC content: 63.09

Gene sequence:

>699_bases
ATGACCTTCAAGGCGACCGTTCTGACGCTCTACCCGGAAATGTTTCCGGGGCATCTCCAATATTCGCTAGCCGGCAAGGC
GCTGGAGCGAGGGCAGTGGTCGCTGGACCCCATACAAATCCGCGAATTTGCCACCGACAGGCACAGAAGCGTCGATGACA
CGCCGGCAGGCGGCGGCGCCGGCATGGTGCTGAAACCCGACGTATTGGCCGCGGCCATCGATCATGTTTCGAAAGGCGAC
ACCCGGCCACGGCTGCTGATGAGCCCGCGCGGCAAACCGCTGTCCCAGAACCGCGTGCGCGAACTGGCGGCCGGCGACGG
CGCGATCATCGTCTGCGGCCGCTTCGAGGGCGTGGACCAGCGCGTCATCGAGGCACGTGGGCTGGAGGAAGTATCAATCG
GCGATTACATTCTCTCCGGCGGCGAACCGGCGGCGCTGACGCTGCTGGATGCTGTCGTCCGCATCCTGCCGGGCGTCATG
GGCAACGATCTCTCTGGCGTGCATGAAAGCTTCGAGGGCGGGTTGCTGGAACATCCGCATTATACCCGCCCGCAACTCTG
GGAAGGCCGCGATATTCCCGCCATCCTCACGTCAGGCAATCACGCCGCTATCGACAAGTGGCGACACGAGCAGGCGCTGG
CACTGACGAAGGAAAGACGGCCGGATCTGCTGGAAAAAACTCAGGCCGAGACGAAATAA

Upstream 100 bases:

>100_bases
GCCGGGCGCATTCTCATCGACCCAATGGCCGCCGGCCTGATCGACAATCCTGACGACAAGGACCAAAACGGCATGTCGCC
GTTCGGTAAGAAATAAATCC

Downstream 100 bases:

>100_bases
TAGGCCGTCAGTACTAGCCCGACTGCAATGACAAGCCAGCGGATGATCCATTGCGGCACCCGTCTTGCAGTGTGCACGCC
CACATAGCCCCCAAGTGCTG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MTFKATVLTLYPEMFPGHLQYSLAGKALERGQWSLDPIQIREFATDRHRSVDDTPAGGGAGMVLKPDVLAAAIDHVSKGD
TRPRLLMSPRGKPLSQNRVRELAAGDGAIIVCGRFEGVDQRVIEARGLEEVSIGDYILSGGEPAALTLLDAVVRILPGVM
GNDLSGVHESFEGGLLEHPHYTRPQLWEGRDIPAILTSGNHAAIDKWRHEQALALTKERRPDLLEKTQAETK

Sequences:

>Translated_232_residues
MTFKATVLTLYPEMFPGHLQYSLAGKALERGQWSLDPIQIREFATDRHRSVDDTPAGGGAGMVLKPDVLAAAIDHVSKGD
TRPRLLMSPRGKPLSQNRVRELAAGDGAIIVCGRFEGVDQRVIEARGLEEVSIGDYILSGGEPAALTLLDAVVRILPGVM
GNDLSGVHESFEGGLLEHPHYTRPQLWEGRDIPAILTSGNHAAIDKWRHEQALALTKERRPDLLEKTQAETK
>Mature_231_residues
TFKATVLTLYPEMFPGHLQYSLAGKALERGQWSLDPIQIREFATDRHRSVDDTPAGGGAGMVLKPDVLAAAIDHVSKGDT
RPRLLMSPRGKPLSQNRVRELAAGDGAIIVCGRFEGVDQRVIEARGLEEVSIGDYILSGGEPAALTLLDAVVRILPGVMG
NDLSGVHESFEGGLLEHPHYTRPQLWEGRDIPAILTSGNHAAIDKWRHEQALALTKERRPDLLEKTQAETK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=222, Percent_Identity=46.3963963963964, Blast_Score=199, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_AGRT5 (Q8UBZ7)

Other databases:

- EMBL:   AE007869
- PIR:   AD2908
- PIR:   D97683
- RefSeq:   NP_355636.1
- ProteinModelPortal:   Q8UBZ7
- SMR:   Q8UBZ7
- STRING:   Q8UBZ7
- GeneID:   1134739
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2701
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q8UBZ7
- ProtClustDB:   PRK00026
- BioCyc:   ATUM176299-1:ATU2701-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25340; Mature: 25209

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKATVLTLYPEMFPGHLQYSLAGKALERGQWSLDPIQIREFATDRHRSVDDTPAGGGA
CCCEEEHEEECHHHCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
GMVLKPDVLAAAIDHVSKGDTRPRLLMSPRGKPLSQNRVRELAAGDGAIIVCGRFEGVDQ
CEEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHH
RVIEARGLEEVSIGDYILSGGEPAALTLLDAVVRILPGVMGNDLSGVHESFEGGLLEHPH
HHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCC
YTRPQLWEGRDIPAILTSGNHAAIDKWRHEQALALTKERRPDLLEKTQAETK
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure 
TFKATVLTLYPEMFPGHLQYSLAGKALERGQWSLDPIQIREFATDRHRSVDDTPAGGGA
CCEEEHEEECHHHCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
GMVLKPDVLAAAIDHVSKGDTRPRLLMSPRGKPLSQNRVRELAAGDGAIIVCGRFEGVDQ
CEEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHH
RVIEARGLEEVSIGDYILSGGEPAALTLLDAVVRILPGVMGNDLSGVHESFEGGLLEHPH
HHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCC
YTRPQLWEGRDIPAILTSGNHAAIDKWRHEQALALTKERRPDLLEKTQAETK
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194