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The map label for this gene is dapF

Identifier: 15889949

GI number: 15889949

Start: 2679613

End: 2680512

Strand: Reverse

Name: dapF

Synonym: Atu2695

Alternate gene names: 15889949

Gene position: 2680512-2679613 (Counterclockwise)

Preceding gene: 159185332

Following gene: 159185331

Centisome position: 94.33

GC content: 61.78

Gene sequence:

>900_bases
ATGGCGGACACGGTCGAATTTGCGAAGATGAACGGGCTTGCCAACAAGATCCTCGTTGTCGATATGCGCGGCCGCAAGGA
TATGGTCACGCCAGCGGCTGCCATCGCCCTCAATTCCAATCCCGCCACGCAGTTCGACCAGATCATGGCGATCCACGATC
CGCGTGTCGGCGGCACGGATGCTTTCATCGATATTCTCAATTGCGATGGCACCAAGGCACAGGCTTGCGGCAACGGCACG
CGCTGCGTGGTGCAGGCGCTGTCTTCCGAAACCGGCAAGACCAGCTTCACCTTCCAGACGGTCGCTGGCATTCTGAACGC
CGTCGAGCATGAAGACGGCATGATATCGGTTGATATGGGCCTGCCGCGTTTCCGCTGGAGCGACATTCCGCTGTCTGAAG
AATTCCACGATACGAGCCGCATCGAATTGCAGATCGGGCCGATCGATGCGCCGATCCTGCATTCGCCTGCGGTCATGTCC
ATGGGCAACCCGCATGCGATTTTCTGGGTTGATCGCGATCCGATGACGTTTGATCTCGAACGTTTCGGGCCGCTTCTCGA
AAATCATCCGATGTTTCCGGAAAAGGCCAATATCACGCTGGCGCAGGTGATTTCCGACAGCGCACTTCGCACCCGCACCT
GGGAGCGCGGCGCGGGGTTGACGCTTGCCTGCGGTTCCGCCGCCTGTGCGTCGGCTGTTTCCGCTGCCCGCACCGGCCGC
ACCGGCCGAAAGGTCACCATCGATGTCGCCTCCAGCCCCGTGCGCGTGCCGCTGACCATCGACTGGCGCGAGGATAATCA
CGTCGTCATGACCGGCCCGGCCGAATGGGAATGGTCGGGTTCCGTCGATCCCGTCACCGGCGTCTGGGCGCGGGATGCGG
TCGAGCGGGGGGCTGTATGA

Upstream 100 bases:

>100_bases
ACAGTCTGGTAATTTCCCGTCCTTTCCGCCATATAGGGCGAAAGCGCGGGAAAATCCCGTATGCAACTCGGTTTTCAGGC
AGTTTTCAGGTTTTAAAGCG

Downstream 100 bases:

>100_bases
GTGGCGTCGAGGTCATAACCTTCGGCTGCCGTCTCAACACCTATGAATCGGAAGTGATGCGGGCGGAGGCCGAAAAGGCG
GGGCTCAACAATGCCGTGCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MADTVEFAKMNGLANKILVVDMRGRKDMVTPAAAIALNSNPATQFDQIMAIHDPRVGGTDAFIDILNCDGTKAQACGNGT
RCVVQALSSETGKTSFTFQTVAGILNAVEHEDGMISVDMGLPRFRWSDIPLSEEFHDTSRIELQIGPIDAPILHSPAVMS
MGNPHAIFWVDRDPMTFDLERFGPLLENHPMFPEKANITLAQVISDSALRTRTWERGAGLTLACGSAACASAVSAARTGR
TGRKVTIDVASSPVRVPLTIDWREDNHVVMTGPAEWEWSGSVDPVTGVWARDAVERGAV

Sequences:

>Translated_299_residues
MADTVEFAKMNGLANKILVVDMRGRKDMVTPAAAIALNSNPATQFDQIMAIHDPRVGGTDAFIDILNCDGTKAQACGNGT
RCVVQALSSETGKTSFTFQTVAGILNAVEHEDGMISVDMGLPRFRWSDIPLSEEFHDTSRIELQIGPIDAPILHSPAVMS
MGNPHAIFWVDRDPMTFDLERFGPLLENHPMFPEKANITLAQVISDSALRTRTWERGAGLTLACGSAACASAVSAARTGR
TGRKVTIDVASSPVRVPLTIDWREDNHVVMTGPAEWEWSGSVDPVTGVWARDAVERGAV
>Mature_298_residues
ADTVEFAKMNGLANKILVVDMRGRKDMVTPAAAIALNSNPATQFDQIMAIHDPRVGGTDAFIDILNCDGTKAQACGNGTR
CVVQALSSETGKTSFTFQTVAGILNAVEHEDGMISVDMGLPRFRWSDIPLSEEFHDTSRIELQIGPIDAPILHSPAVMSM
GNPHAIFWVDRDPMTFDLERFGPLLENHPMFPEKANITLAQVISDSALRTRTWERGAGLTLACGSAACASAVSAARTGRT
GRKVTIDVASSPVRVPLTIDWREDNHVVMTGPAEWEWSGSVDPVTGVWARDAVERGAV

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=282, Percent_Identity=33.3333333333333, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_AGRT5 (Q8UC03)

Other databases:

- EMBL:   AE007869
- PIR:   AF2907
- PIR:   F97682
- RefSeq:   NP_355630.1
- ProteinModelPortal:   Q8UC03
- SMR:   Q8UC03
- STRING:   Q8UC03
- GeneID:   1134733
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2695
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   TRTWERG
- PhylomeDB:   Q8UC03
- ProtClustDB:   PRK00450
- BioCyc:   ATUM176299-1:ATU2695-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 32287; Mature: 32156

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 76-76 ACT_SITE 224-224

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTVEFAKMNGLANKILVVDMRGRKDMVTPAAAIALNSNPATQFDQIMAIHDPRVGGTD
CCCCHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHEEEEECCCCCCCC
AFIDILNCDGTKAQACGNGTRCVVQALSSETGKTSFTFQTVAGILNAVEHEDGMISVDMG
EEEEEEECCCCCHHHCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCEEEEECC
LPRFRWSDIPLSEEFHDTSRIELQIGPIDAPILHSPAVMSMGNPHAIFWVDRDPMTFDLE
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEECCCCEEEHH
RFGPLLENHPMFPEKANITLAQVISDSALRTRTWERGAGLTLACGSAACASAVSAARTGR
HHCCHHHCCCCCCCCCCEEHHHHHCCCHHHHCCCCCCCCEEEEECCHHHHHHHHHHHCCC
TGRKVTIDVASSPVRVPLTIDWREDNHVVMTGPAEWEWSGSVDPVTGVWARDAVERGAV
CCCEEEEEECCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ADTVEFAKMNGLANKILVVDMRGRKDMVTPAAAIALNSNPATQFDQIMAIHDPRVGGTD
CCCHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHEEEEECCCCCCCC
AFIDILNCDGTKAQACGNGTRCVVQALSSETGKTSFTFQTVAGILNAVEHEDGMISVDMG
EEEEEEECCCCCHHHCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCEEEEECC
LPRFRWSDIPLSEEFHDTSRIELQIGPIDAPILHSPAVMSMGNPHAIFWVDRDPMTFDLE
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEECCCCEEEHH
RFGPLLENHPMFPEKANITLAQVISDSALRTRTWERGAGLTLACGSAACASAVSAARTGR
HHCCHHHCCCCCCCCCCEEHHHHHCCCHHHHCCCCCCCCEEEEECCHHHHHHHHHHHCCC
TGRKVTIDVASSPVRVPLTIDWREDNHVVMTGPAEWEWSGSVDPVTGVWARDAVERGAV
CCCEEEEEECCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194