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The map label for this gene is sucB [H]

Identifier: 15889890

GI number: 15889890

Start: 2618190

End: 2619422

Strand: Reverse

Name: sucB [H]

Synonym: Atu2635

Alternate gene names: 15889890

Gene position: 2619422-2618190 (Counterclockwise)

Preceding gene: 159185304

Following gene: 159185303

Centisome position: 92.18

GC content: 62.69

Gene sequence:

>1233_bases
ATGGCCACTGAAATCCGCGTACCAACCCTCGGCGAATCCGTCAGCGAAGCGACCGTCGGCACCTGGTTCAAGAAGGTCGG
CGATACCGTCAAGGCCGACGAACCTCTCGTTGAACTCGAAACCGACAAGGTTACCGTCGAAGTCCCGGCCCCTGCGTCAG
GCGTTCTCACCGAAATCGTTGCGCAGAACGGCGAGACCGTTGGTCTCGACGCGCTTCTCGGCCAGATCGCCGAAGGTGCT
GCCGGCGCTGCGACTTCTGCACCTGCGGCAAAGCCTGCTGCGCCCGCCGCCGCACCGGCACCTGCCGCAGCCGTAGCCGC
CGCTCCGGGTGGCAGCGCCATGCCACCGGCACCTGCCGCTGGCAAGCTGCTTGCCGAAAACAACCTGTCTGCCGATCAGG
TTGACGGTTCGGGCAAGCGTGGTCAGGTTCTGAAGGGCGACGTTCTTGCCGCTGTCGCCAAGGGCGTTTCGGCACCTGCC
GCCGCACCGGCACCGGTTGCCGCCCCCCGTCCGGTTTCGGCCGAGCAGGATCAGGTTCGCGAAGAGCGCGTCAAGATGAC
CCGTCTGCGCCAGACGATCGCCCGCCGCCTGAAGGACGCGCAGAACACCGCCGCCATGCTCACCACTTACAACGAAGTGG
ACATGAGCGCGGTCATGGACCTTCGTAACCGCTACAAGGACGTGTTTGAAAAGAAGCACGGCGTCAAGCTTGGCTTCATG
GGCTTCTTCACCAAAGCCGTGACCCACGCGCTGAAGGAGCTGCCTGCTGTCAACGCTGAAATCGACGGCACGGACATCAT
CTACAAGAACTATTGTCACGTCGGCATGGCTGTCGGCACCGACAAGGGCCTCGTCGTTCCTGTTATCCGTGATGCCGACC
AGCTCTCCATCGCCGGCGTCGAAAAGGAACTCGGTCGCCTTGCCAAGGCAGCCCGTGACGGTTCGCTCGGCATGGCGGAC
ATGCAGGGCGGCACCTTCACCATCACCAATGGTGGCGTCTACGGTTCACTAATGTCTTCGCCGATCCTCAACGCACCGCA
GTCGGGCATTCTCGGCATGCACAAGATCCAGGAGCGCCCGGTCGCCATCGGCGGTCAGGTCGTCATCCGCCCGATGATGT
ATCTTGCGCTCTCCTACGATCACCGTATCGTTGACGGCAAGGAAGCCGTGACCTTCCTCGTTCGCGTCAAGGAGAGCCTC
GAGGATCCGGAACGTCTGGTTCTCGATCTCTGA

Upstream 100 bases:

>100_bases
ATCTGGCGCAGCTTGCTGCGTTCCTGGAAGACGCGCTGGGAGAGTGAGAACTCTCCCGCATCGCCCCGTAAAAAAGACAC
CCGAATAACGGAACTAGATC

Downstream 100 bases:

>100_bases
TCGGTATTGCTTTTCGTTTCAGCGGGGCTTCAAGTGAACCCCGCTGAAAGACCACCCGGGAGAGCAGACGTGCAGCCGAC
ATTTCTTGCCGCTTCGCCTT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MATEIRVPTLGESVSEATVGTWFKKVGDTVKADEPLVELETDKVTVEVPAPASGVLTEIVAQNGETVGLDALLGQIAEGA
AGAATSAPAAKPAAPAAAPAPAAAVAAAPGGSAMPPAPAAGKLLAENNLSADQVDGSGKRGQVLKGDVLAAVAKGVSAPA
AAPAPVAAPRPVSAEQDQVREERVKMTRLRQTIARRLKDAQNTAAMLTTYNEVDMSAVMDLRNRYKDVFEKKHGVKLGFM
GFFTKAVTHALKELPAVNAEIDGTDIIYKNYCHVGMAVGTDKGLVVPVIRDADQLSIAGVEKELGRLAKAARDGSLGMAD
MQGGTFTITNGGVYGSLMSSPILNAPQSGILGMHKIQERPVAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESL
EDPERLVLDL

Sequences:

>Translated_410_residues
MATEIRVPTLGESVSEATVGTWFKKVGDTVKADEPLVELETDKVTVEVPAPASGVLTEIVAQNGETVGLDALLGQIAEGA
AGAATSAPAAKPAAPAAAPAPAAAVAAAPGGSAMPPAPAAGKLLAENNLSADQVDGSGKRGQVLKGDVLAAVAKGVSAPA
AAPAPVAAPRPVSAEQDQVREERVKMTRLRQTIARRLKDAQNTAAMLTTYNEVDMSAVMDLRNRYKDVFEKKHGVKLGFM
GFFTKAVTHALKELPAVNAEIDGTDIIYKNYCHVGMAVGTDKGLVVPVIRDADQLSIAGVEKELGRLAKAARDGSLGMAD
MQGGTFTITNGGVYGSLMSSPILNAPQSGILGMHKIQERPVAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESL
EDPERLVLDL
>Mature_409_residues
ATEIRVPTLGESVSEATVGTWFKKVGDTVKADEPLVELETDKVTVEVPAPASGVLTEIVAQNGETVGLDALLGQIAEGAA
GAATSAPAAKPAAPAAAPAPAAAVAAAPGGSAMPPAPAAGKLLAENNLSADQVDGSGKRGQVLKGDVLAAVAKGVSAPAA
APAPVAAPRPVSAEQDQVREERVKMTRLRQTIARRLKDAQNTAAMLTTYNEVDMSAVMDLRNRYKDVFEKKHGVKLGFMG
FFTKAVTHALKELPAVNAEIDGTDIIYKNYCHVGMAVGTDKGLVVPVIRDADQLSIAGVEKELGRLAKAARDGSLGMADM
QGGTFTITNGGVYGSLMSSPILNAPQSGILGMHKIQERPVAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESLE
DPERLVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=54.7244094488189, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=28.3410138248848, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=28.8888888888889, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=28.8888888888889, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI31711992, Length=438, Percent_Identity=28.310502283105, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.1614906832298, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786946, Length=410, Percent_Identity=51.7073170731707, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=32.9411764705882, Blast_Score=182, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI25146366, Length=409, Percent_Identity=43.2762836185819, Blast_Score=327, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=31.5192743764172, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=423, Percent_Identity=28.6052009456265, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=28.3387622149837, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=415, Percent_Identity=45.5421686746988, Blast_Score=352, Evalue=9e-98,
Organism=Saccharomyces cerevisiae, GI6324258, Length=458, Percent_Identity=24.235807860262, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=58.5903083700441, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI18859875, Length=424, Percent_Identity=30.188679245283, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=29.8701298701299, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=29.8701298701299, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42687; Mature: 42555

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEIRVPTLGESVSEATVGTWFKKVGDTVKADEPLVELETDKVTVEVPAPASGVLTEIV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHHH
AQNGETVGLDALLGQIAEGAAGAATSAPAAKPAAPAAAPAPAAAVAAAPGGSAMPPAPAA
HCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCC
GKLLAENNLSADQVDGSGKRGQVLKGDVLAAVAKGVSAPAAAPAPVAAPRPVSAEQDQVR
CCEEECCCCCHHCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
EERVKMTRLRQTIARRLKDAQNTAAMLTTYNEVDMSAVMDLRNRYKDVFEKKHGVKLGFM
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEHHH
GFFTKAVTHALKELPAVNAEIDGTDIIYKNYCHVGMAVGTDKGLVVPVIRDADQLSIAGV
HHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEEEECCCCHHHHHHH
EKELGRLAKAARDGSLGMADMQGGTFTITNGGVYGSLMSSPILNAPQSGILGMHKIQERP
HHHHHHHHHHHCCCCCCEEECCCCEEEEECCCEEHHHHCCCCCCCCCCCCCCHHHHHCCC
VAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDPERLVLDL
CEECCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHHEECC
>Mature Secondary Structure 
ATEIRVPTLGESVSEATVGTWFKKVGDTVKADEPLVELETDKVTVEVPAPASGVLTEIV
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHHH
AQNGETVGLDALLGQIAEGAAGAATSAPAAKPAAPAAAPAPAAAVAAAPGGSAMPPAPAA
HCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCC
GKLLAENNLSADQVDGSGKRGQVLKGDVLAAVAKGVSAPAAAPAPVAAPRPVSAEQDQVR
CCEEECCCCCHHCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
EERVKMTRLRQTIARRLKDAQNTAAMLTTYNEVDMSAVMDLRNRYKDVFEKKHGVKLGFM
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEHHH
GFFTKAVTHALKELPAVNAEIDGTDIIYKNYCHVGMAVGTDKGLVVPVIRDADQLSIAGV
HHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEEEECCCCHHHHHHH
EKELGRLAKAARDGSLGMADMQGGTFTITNGGVYGSLMSSPILNAPQSGILGMHKIQERP
HHHHHHHHHHHCCCCCCEEECCCCEEEEECCCEEHHHHCCCCCCCCCCCCCCHHHHHCCC
VAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDPERLVLDL
CEECCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]