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The map label for this gene is gcvA [H]

Identifier: 15889589

GI number: 15889589

Start: 2290087

End: 2291016

Strand: Direct

Name: gcvA [H]

Synonym: Atu2312

Alternate gene names: 15889589

Gene position: 2290087-2291016 (Clockwise)

Preceding gene: 15889588

Following gene: 159185155

Centisome position: 80.59

GC content: 60.11

Gene sequence:

>930_bases
ATGAAGATGTCTCGCCAGTTCCCTCTGAATGCGCTGCGCGTCTTCGATGCCGCCGCCAGGCACCTGAGTTTCACCAAGGC
GGGTGAAGAGCTGGGCATGACGCAGACCGCGGTGAGTTACCAGGTGAAGCTCTTGGAGGAGAATATCGGTGAGCCGCTCT
TCATCCGCAAAGCGCGCCAGGTTCAGCTGACCGAGGCCGGGCAGAAACTGGCGCCTAAAGTGGCGGAAGCCTTCCACAGC
CTGCGGGAGGCCGTCGACAATGTGCGCGACACATCCGACACCACCCTGACCATCCATTCCACCGCCACTTTCGCATCGCG
CTGGCTGTCGCGGCATCTCGGCGCTTTCCAGCTTGAACATCCCTCGATCGCGGTGCGGCTCGATACATCAAGCGCATTGA
TCGATTTTTCCCAGTCGGACTGCGATGTCGCCATTCGCTGGAGCCGCGACGATGGCAAGGGGCTGGCCTATCATCAATTG
CTGCGCGGCGTTTATACGCCGATGCTGCATCCGAGCCTCGCCGAGAGCATCGGCGGATTGCACAGGCCGGAAGATCTGCT
GCGCCTGCGCATCATCGATCCCGGCGACATATGGTGGAGCCAGTGGTTTCGTGAGGTGGGCGTCGAAAATCCGGGCCTTG
ACCGTTATCCACGCAGCCGCCTGAATGTGCAGGCCTTCGAAGCCGCCGCGGCAATCGCCAATCAGGGGGTGGCCATGCTC
ACCCCGGAGCTTTATGCCGACGAGATCGCGCTCGGCCGCCTTTACCAGCCATTCGAGCACCTCAGCAGCGAAGGCAAGAA
CTACTGGCTGGTCTATCCGGAAAACCGCAGGAACATTCGCAAGATAAAACTGTTTCGCGACTGGATATTGAAGCGCATAG
AGGAAAGCCGGCCGCAGCAGCAGCCTCAATATCCCATATCAGGCGCATAA

Upstream 100 bases:

>100_bases
AAATGTCAGAAGCTCAGTTGATGAATTGGGCTAAAAATGCTCTGGTTTCGGGAGAAAAACCAATGAAACCTTTGGCGACT
AGCATAAAGAGAACTTATCC

Downstream 100 bases:

>100_bases
AAGCAGCGCGGCGTATCCTCGTCCGGAAACAGACAGAGATGATATTCACCATCTTCGGACCGCCTTGCCTCGCTCTGCGA
CCAGCTGAAGACATGGCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKMSRQFPLNALRVFDAAARHLSFTKAGEELGMTQTAVSYQVKLLEENIGEPLFIRKARQVQLTEAGQKLAPKVAEAFHS
LREAVDNVRDTSDTTLTIHSTATFASRWLSRHLGAFQLEHPSIAVRLDTSSALIDFSQSDCDVAIRWSRDDGKGLAYHQL
LRGVYTPMLHPSLAESIGGLHRPEDLLRLRIIDPGDIWWSQWFREVGVENPGLDRYPRSRLNVQAFEAAAAIANQGVAML
TPELYADEIALGRLYQPFEHLSSEGKNYWLVYPENRRNIRKIKLFRDWILKRIEESRPQQQPQYPISGA

Sequences:

>Translated_309_residues
MKMSRQFPLNALRVFDAAARHLSFTKAGEELGMTQTAVSYQVKLLEENIGEPLFIRKARQVQLTEAGQKLAPKVAEAFHS
LREAVDNVRDTSDTTLTIHSTATFASRWLSRHLGAFQLEHPSIAVRLDTSSALIDFSQSDCDVAIRWSRDDGKGLAYHQL
LRGVYTPMLHPSLAESIGGLHRPEDLLRLRIIDPGDIWWSQWFREVGVENPGLDRYPRSRLNVQAFEAAAAIANQGVAML
TPELYADEIALGRLYQPFEHLSSEGKNYWLVYPENRRNIRKIKLFRDWILKRIEESRPQQQPQYPISGA
>Mature_309_residues
MKMSRQFPLNALRVFDAAARHLSFTKAGEELGMTQTAVSYQVKLLEENIGEPLFIRKARQVQLTEAGQKLAPKVAEAFHS
LREAVDNVRDTSDTTLTIHSTATFASRWLSRHLGAFQLEHPSIAVRLDTSSALIDFSQSDCDVAIRWSRDDGKGLAYHQL
LRGVYTPMLHPSLAESIGGLHRPEDLLRLRIIDPGDIWWSQWFREVGVENPGLDRYPRSRLNVQAFEAAAAIANQGVAML
TPELYADEIALGRLYQPFEHLSSEGKNYWLVYPENRRNIRKIKLFRDWILKRIEESRPQQQPQYPISGA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=36.518771331058, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1786448, Length=302, Percent_Identity=31.7880794701987, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=26.6666666666667, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787128, Length=305, Percent_Identity=27.5409836065574, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI157672245, Length=170, Percent_Identity=32.3529411764706, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790208, Length=215, Percent_Identity=26.046511627907, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87081978, Length=155, Percent_Identity=29.0322580645161, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI145693105, Length=161, Percent_Identity=27.9503105590062, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786508, Length=168, Percent_Identity=26.1904761904762, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786401, Length=138, Percent_Identity=29.7101449275362, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788748, Length=65, Percent_Identity=47.6923076923077, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35192; Mature: 35192

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMSRQFPLNALRVFDAAARHLSFTKAGEELGMTQTAVSYQVKLLEENIGEPLFIRKARQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEHHHHH
VQLTEAGQKLAPKVAEAFHSLREAVDNVRDTSDTTLTIHSTATFASRWLSRHLGAFQLEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCCEEECC
PSIAVRLDTSSALIDFSQSDCDVAIRWSRDDGKGLAYHQLLRGVYTPMLHPSLAESIGGL
CEEEEEECCCCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCC
HRPEDLLRLRIIDPGDIWWSQWFREVGVENPGLDRYPRSRLNVQAFEAAAAIANQGVAML
CCCCHHEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCEEEE
TPELYADEIALGRLYQPFEHLSSEGKNYWLVYPENRRNIRKIKLFRDWILKRIEESRPQQ
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
QPQYPISGA
CCCCCCCCC
>Mature Secondary Structure
MKMSRQFPLNALRVFDAAARHLSFTKAGEELGMTQTAVSYQVKLLEENIGEPLFIRKARQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEHHHHH
VQLTEAGQKLAPKVAEAFHSLREAVDNVRDTSDTTLTIHSTATFASRWLSRHLGAFQLEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCCEEECC
PSIAVRLDTSSALIDFSQSDCDVAIRWSRDDGKGLAYHQLLRGVYTPMLHPSLAESIGGL
CEEEEEECCCCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCC
HRPEDLLRLRIIDPGDIWWSQWFREVGVENPGLDRYPRSRLNVQAFEAAAAIANQGVAML
CCCCHHEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCEEEE
TPELYADEIALGRLYQPFEHLSSEGKNYWLVYPENRRNIRKIKLFRDWILKRIEESRPQQ
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
QPQYPISGA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]