Click here to switch to the map view.

The map label for this gene is trpE(G) [H]

Identifier: 15889565

GI number: 15889565

Start: 2262145

End: 2264334

Strand: Direct

Name: trpE(G) [H]

Synonym: Atu2289

Alternate gene names: 15889565

Gene position: 2262145-2264334 (Clockwise)

Preceding gene: 159185142

Following gene: 159185143

Centisome position: 79.61

GC content: 60.32

Gene sequence:

>2190_bases
ATGGTAACGATCATTCAGGATGACGGAGCGGAGACCTACGAGACGAAAGGCGGCATCCAGGTCAGCCGAAAGCGCCGGCC
CACCGATTATGCCAACGCCATCGATAATTACATCGAAAAGCTTGATTCCCATCGCGGCGCGGTTTTTTCGTCCAACTATG
AATATCCGGGCCGTTACACCCGCTGGGATACGGCCATCGTCGATCCGCCGCTCGGCATTTCCTGTTTTGGCCGCAAGATG
TGGATCGAAGCCTATAATGGCCGCGGCGAAGTGCTGCTCGATTTCATTACGGAAAAGCTGAAGGCGACACCCGATCTCAC
CCTCGGCGCTTCCTCGACCCGCCGGCTCGATCTTACCGTCAACGAACCGGACCGTGTCTTCACCGAAGAAGAACGCTCGA
AAATCCCGACGGTCTTCACCGCTCTCAGAGCCATCGTCGACCTCTTCTATTCGAGCGCGGATTCGGCCATCGGCCTGTTC
GGTGCCTTCGGTTACGATCTCGCCTTCCAGTTCGACGCGATCAAGCTTTCGCTGGCGCGTCCGGAAGACCAGCGTGACAT
GGTGCTGTTTCTGCCCGATGAAATCCTCGTCGTTGATCACTATTCCGCCAAGGCCTGGATCGACCGTTACGATTTCGAGA
AGGACGGCATGACGACGGACGGCAAATCCTCCGACATTACCCCCGATCCCTTCAAGACCACCGATACCATCCCGCCCAAG
GGCGATCACCGTCCCGGCGAATATTCCGAGCTTGTGGTGAAGGCCAAGGAAAGCTTCCGCCGCGGCGACCTGTTCGAGGT
CGTTCCCGGCCAGAAATTCATGGAGCGTTGCGAAAGCAATCCGTCGGCGATTTCCCGCCGCCTGAAGGCGATCAACCCGT
CGCCCTATTCCTTCTTCATCAATCTCGGCGATCAGGAATATCTGGTCGGCGCCTCGCCGGAAATGTTCGTGCGCGTCTCC
GGCCGTCGCATCGAGACCTGCCCGATATCAGGCACCATCAAGCGCGGCGACGATCCGATTGCCGACAGCGAGCAGATTTT
GAAACTGCTCAACTCGAAAAAGGACGAATCCGAACTGACCATGTGCTCGGACGTGGACCGCAACGACAAGAGCCGCGTCT
GCGAGCCGGGTTCGGTGAAGGTCATTGGCCGCCGCCAGATCGAGATGTATTCACGCCTCATCCACACCGTCGATCACATC
GAAGGCCGCCTGCGCGACGATATGGACGCCTTTGACGGTTTCCTCAGCCACGCCTGGGCCGTCACCGTCACCGGTGCACC
AAAGCTGTGGGCCATGCGCTTCATCGAAGGTCATGAAAAGAGCCCGCGCGCCTGGTATGGCGGTGCGATCGGCATGGTCG
GCTTCAACGGCGACATGAATACCGGCCTGACGCTGCGCACCATCCGGATCAAGGACGGTATTGCCGAAGTGCGCGCCGGC
GCGACCCTGCTCAATGATTCCAACCCGCAGGAAGAAGAAGCCGAAACCGAACTGAAGGCCTCCGCCATGATATCAGCCAT
TCGTGACGCAAAAGGCACCAACTCTGCCGCCACCAAGCGTGATGCCGCCAAAGTCGGCACCGGCGTCAAGATCCTGCTCG
TCGACCACGAAGACAGCTTCGTGCACACGCTGGCGAATTATTTCCGCCAGACGGGCGCGACGGTCTCGACCGTCAGATCA
CCGGTCGCAGCCGACGTGTTCGATCGCTTCCAGCCGGACCTCGTTGTCCTGTCGCCCGGACCCGGCAGCCCGACGGATTT
CGACTGCAAGGCAACGATCAAGGCCGCCCGCGCCCGCGATCTGCCGATCTTCGGCGTTTGCCTCGGTCTGCAGGCATTGG
CAGAAGCCTATGGCGGCGAGCTGCGCCAGCTTGCTGTGCCCATGCACGGCAAGCCTTCGCGCATCCGCGTGCTGGAACCC
GGCCTCGTCTTCTCCGGTCTCGGCAAGGAAGTCACGGTCGGTCGTTACCATTCGATCTTCGCCGATCCCGCCACCCTGCC
GCGTGATTTCATCATCACCGCAGAAAGCGAGGACGGCACGATCATGGGCATCGAACACGCCAAGGAACCGGTGGCCGCCG
TTCAGTTCCACCCGGAATCGATCATGACGCTCGGACAGGACGCGGGCATGCGGATGATCGAGAATGTCGTGGTGCATCTG
ACCCGCAAGGCGAAGACCAAGGCCGCGTGA

Upstream 100 bases:

>100_bases
CATGTCTTCAGACAAAGTCCAAGCCGCCCGAATTTTCAGGCGGCTTTTTTGTTATTATGCCGCCTGTCATCGGGCCTGAA
CAACAGGAAGTACGAGAGAA

Downstream 100 bases:

>100_bases
TGGCGCTCGATGACACGGTTACCATTAAGCCTATCCGGGCGGAGCACGTTGAAAGCTTTCATCGTGCTCTCGATGCAGTG
TCGCGTGAGCGGAAATATCT

Product: anthranilate synthase

Products: NA

Alternate protein names: Glutamine amidotransferase [H]

Number of amino acids: Translated: 729; Mature: 729

Protein sequence:

>729_residues
MVTIIQDDGAETYETKGGIQVSRKRRPTDYANAIDNYIEKLDSHRGAVFSSNYEYPGRYTRWDTAIVDPPLGISCFGRKM
WIEAYNGRGEVLLDFITEKLKATPDLTLGASSTRRLDLTVNEPDRVFTEEERSKIPTVFTALRAIVDLFYSSADSAIGLF
GAFGYDLAFQFDAIKLSLARPEDQRDMVLFLPDEILVVDHYSAKAWIDRYDFEKDGMTTDGKSSDITPDPFKTTDTIPPK
GDHRPGEYSELVVKAKESFRRGDLFEVVPGQKFMERCESNPSAISRRLKAINPSPYSFFINLGDQEYLVGASPEMFVRVS
GRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVKVIGRRQIEMYSRLIHTVDHI
EGRLRDDMDAFDGFLSHAWAVTVTGAPKLWAMRFIEGHEKSPRAWYGGAIGMVGFNGDMNTGLTLRTIRIKDGIAEVRAG
ATLLNDSNPQEEEAETELKASAMISAIRDAKGTNSAATKRDAAKVGTGVKILLVDHEDSFVHTLANYFRQTGATVSTVRS
PVAADVFDRFQPDLVVLSPGPGSPTDFDCKATIKAARARDLPIFGVCLGLQALAEAYGGELRQLAVPMHGKPSRIRVLEP
GLVFSGLGKEVTVGRYHSIFADPATLPRDFIITAESEDGTIMGIEHAKEPVAAVQFHPESIMTLGQDAGMRMIENVVVHL
TRKAKTKAA

Sequences:

>Translated_729_residues
MVTIIQDDGAETYETKGGIQVSRKRRPTDYANAIDNYIEKLDSHRGAVFSSNYEYPGRYTRWDTAIVDPPLGISCFGRKM
WIEAYNGRGEVLLDFITEKLKATPDLTLGASSTRRLDLTVNEPDRVFTEEERSKIPTVFTALRAIVDLFYSSADSAIGLF
GAFGYDLAFQFDAIKLSLARPEDQRDMVLFLPDEILVVDHYSAKAWIDRYDFEKDGMTTDGKSSDITPDPFKTTDTIPPK
GDHRPGEYSELVVKAKESFRRGDLFEVVPGQKFMERCESNPSAISRRLKAINPSPYSFFINLGDQEYLVGASPEMFVRVS
GRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVKVIGRRQIEMYSRLIHTVDHI
EGRLRDDMDAFDGFLSHAWAVTVTGAPKLWAMRFIEGHEKSPRAWYGGAIGMVGFNGDMNTGLTLRTIRIKDGIAEVRAG
ATLLNDSNPQEEEAETELKASAMISAIRDAKGTNSAATKRDAAKVGTGVKILLVDHEDSFVHTLANYFRQTGATVSTVRS
PVAADVFDRFQPDLVVLSPGPGSPTDFDCKATIKAARARDLPIFGVCLGLQALAEAYGGELRQLAVPMHGKPSRIRVLEP
GLVFSGLGKEVTVGRYHSIFADPATLPRDFIITAESEDGTIMGIEHAKEPVAAVQFHPESIMTLGQDAGMRMIENVVVHL
TRKAKTKAA
>Mature_729_residues
MVTIIQDDGAETYETKGGIQVSRKRRPTDYANAIDNYIEKLDSHRGAVFSSNYEYPGRYTRWDTAIVDPPLGISCFGRKM
WIEAYNGRGEVLLDFITEKLKATPDLTLGASSTRRLDLTVNEPDRVFTEEERSKIPTVFTALRAIVDLFYSSADSAIGLF
GAFGYDLAFQFDAIKLSLARPEDQRDMVLFLPDEILVVDHYSAKAWIDRYDFEKDGMTTDGKSSDITPDPFKTTDTIPPK
GDHRPGEYSELVVKAKESFRRGDLFEVVPGQKFMERCESNPSAISRRLKAINPSPYSFFINLGDQEYLVGASPEMFVRVS
GRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVKVIGRRQIEMYSRLIHTVDHI
EGRLRDDMDAFDGFLSHAWAVTVTGAPKLWAMRFIEGHEKSPRAWYGGAIGMVGFNGDMNTGLTLRTIRIKDGIAEVRAG
ATLLNDSNPQEEEAETELKASAMISAIRDAKGTNSAATKRDAAKVGTGVKILLVDHEDSFVHTLANYFRQTGATVSTVRS
PVAADVFDRFQPDLVVLSPGPGSPTDFDCKATIKAARARDLPIFGVCLGLQALAEAYGGELRQLAVPMHGKPSRIRVLEP
GLVFSGLGKEVTVGRYHSIFADPATLPRDFIITAESEDGTIMGIEHAKEPVAAVQFHPESIMTLGQDAGMRMIENVVVHL
TRKAKTKAA

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=467, Percent_Identity=31.4775160599572, Blast_Score=188, Evalue=9e-49,
Organism=Escherichia coli, GI1788114, Length=365, Percent_Identity=29.5890410958904, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789760, Length=189, Percent_Identity=42.3280423280423, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1787517, Length=197, Percent_Identity=35.5329949238579, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI1786809, Length=224, Percent_Identity=29.4642857142857, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1786215, Length=151, Percent_Identity=31.7880794701987, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=271, Percent_Identity=37.6383763837638, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322638, Length=189, Percent_Identity=35.978835978836, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR006805
- InterPro:   IPR006220
- InterPro:   IPR010112
- InterPro:   IPR001317
- InterPro:   IPR015890
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 80548; Mature: 80548

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTIIQDDGAETYETKGGIQVSRKRRPTDYANAIDNYIEKLDSHRGAVFSSNYEYPGRYT
CEEEEECCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
RWDTAIVDPPLGISCFGRKMWIEAYNGRGEVLLDFITEKLKATPDLTLGASSTRRLDLTV
CCCCEECCCCCCHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCEEEEEEE
NEPDRVFTEEERSKIPTVFTALRAIVDLFYSSADSAIGLFGAFGYDLAFQFDAIKLSLAR
CCCCHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEEEEEEECC
PEDQRDMVLFLPDEILVVDHYSAKAWIDRYDFEKDGMTTDGKSSDITPDPFKTTDTIPPK
CCCCCCEEEEECCEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GDHRPGEYSELVVKAKESFRRGDLFEVVPGQKFMERCESNPSAISRRLKAINPSPYSFFI
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEE
NLGDQEYLVGASPEMFVRVSGRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELT
EECCCEEEECCCCCEEEEECCCEEEECCCCCEEECCCCCCCCHHHHHHHHHCCCCCCHHH
MCSDVDRNDKSRVCEPGSVKVIGRRQIEMYSRLIHTVDHIEGRLRDDMDAFDGFLSHAWA
EHHCCCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VTVTGAPKLWAMRFIEGHEKSPRAWYGGAIGMVGFNGDMNTGLTLRTIRIKDGIAEVRAG
EEECCCCHHHHHHHHHCCCCCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCHHHHHCC
ATLLNDSNPQEEEAETELKASAMISAIRDAKGTNSAATKRDAAKVGTGVKILLVDHEDSF
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCHH
VHTLANYFRQTGATVSTVRSPVAADVFDRFQPDLVVLSPGPGSPTDFDCKATIKAARARD
HHHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHCCC
LPIFGVCLGLQALAEAYGGELRQLAVPMHGKPSRIRVLEPGLVFSGLGKEVTVGRYHSIF
CCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCEEECCCCEEECCHHHHEE
ADPATLPRDFIITAESEDGTIMGIEHAKEPVAAVQFHPESIMTLGQDAGMRMIENVVVHL
CCCCCCCCEEEEEEECCCCEEEEHHHCCCCCEEEEECCHHHHHCCCCCHHHHHHHHHHHH
TRKAKTKAA
HHHHHCCCC
>Mature Secondary Structure
MVTIIQDDGAETYETKGGIQVSRKRRPTDYANAIDNYIEKLDSHRGAVFSSNYEYPGRYT
CEEEEECCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
RWDTAIVDPPLGISCFGRKMWIEAYNGRGEVLLDFITEKLKATPDLTLGASSTRRLDLTV
CCCCEECCCCCCHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCEEEEEEE
NEPDRVFTEEERSKIPTVFTALRAIVDLFYSSADSAIGLFGAFGYDLAFQFDAIKLSLAR
CCCCHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEEEEEEECC
PEDQRDMVLFLPDEILVVDHYSAKAWIDRYDFEKDGMTTDGKSSDITPDPFKTTDTIPPK
CCCCCCEEEEECCEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GDHRPGEYSELVVKAKESFRRGDLFEVVPGQKFMERCESNPSAISRRLKAINPSPYSFFI
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEE
NLGDQEYLVGASPEMFVRVSGRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELT
EECCCEEEECCCCCEEEEECCCEEEECCCCCEEECCCCCCCCHHHHHHHHHCCCCCCHHH
MCSDVDRNDKSRVCEPGSVKVIGRRQIEMYSRLIHTVDHIEGRLRDDMDAFDGFLSHAWA
EHHCCCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VTVTGAPKLWAMRFIEGHEKSPRAWYGGAIGMVGFNGDMNTGLTLRTIRIKDGIAEVRAG
EEECCCCHHHHHHHHHCCCCCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCHHHHHCC
ATLLNDSNPQEEEAETELKASAMISAIRDAKGTNSAATKRDAAKVGTGVKILLVDHEDSF
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCHH
VHTLANYFRQTGATVSTVRSPVAADVFDRFQPDLVVLSPGPGSPTDFDCKATIKAARARD
HHHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHCCC
LPIFGVCLGLQALAEAYGGELRQLAVPMHGKPSRIRVLEPGLVFSGLGKEVTVGRYHSIF
CCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCEEECCCCEEECCHHHHEE
ADPATLPRDFIITAESEDGTIMGIEHAKEPVAAVQFHPESIMTLGQDAGMRMIENVVVHL
CCCCCCCCEEEEEEECCCCEEEEHHHCCCCCEEEEECCHHHHHCCCCCHHHHHHHHHHHH
TRKAKTKAA
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656657; 11481430 [H]