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The map label for this gene is chaA [H]

Identifier: 15889473

GI number: 15889473

Start: 2168453

End: 2169559

Strand: Direct

Name: chaA [H]

Synonym: Atu2195

Alternate gene names: 15889473

Gene position: 2168453-2169559 (Clockwise)

Preceding gene: 159185098

Following gene: 15889475

Centisome position: 76.31

GC content: 60.07

Gene sequence:

>1107_bases
ATGGTGGGTTCTATTTTGAAACAGGAGCGCATGCTGCTCCTGGCGATACCGGCGGCAATAGCGGCCTATATGGTGGAACA
TGCGATATTCGAAGCCGGAAAGACGGCGGGGCTGATTGCCGCAATCGTGCTCATCGGGCTCATCGTTCTCGTCTCGATGC
GGGTTGCCCATCACGCTGAAATTCTCGCCGCCAAGGTCGGCGATCCCTACGGCACGATGATCCTCACACTCTCGGCGGTC
GCCGTGGAAGTGCTGATCCTCGCCATCATCATGAGCGAATCCAGTTCGCCAACGCTGGTGCGCGATACGATCTACTCCGC
CGTCATGATCGACATCAATGGCATCCTGGGGTTGGCCGCCCTGATCGGCGGTCTGCGCCACGGCGAGCAGCCCTATAATG
ACGATTCCGGCAAGACCTATGGTGTGATGATCCTCACCGCCATGGGCATTTCGATGATCGTGCCGGAATTCATTCCGCAA
CAGAGCTGGCACATCTATTCCGTGTTCACCATCGGGGCGATGATCGCGCTCTATGCGCTCTTCCTCAAGATGCAGGTCGG
TCCGCACAGCTATTTCTTCAGCTATACCTATCCCCGCAAGGCGCATCCGGTCGGTCAGGCATCAGACGGGCATGGGCAAG
AGGAAGACGAAGGCAGCGTGCCGCTCTCCATCGGCCTCATCATTGCCGGCGTGGTGCTGATCGGCGTGCTGGCGGAATTC
ATGTCGGCCTTCCTGGGCGAAAGCCTCGAGGGCACGAGCGCCCCGCCCGCGCTGATGGCGGTGGTGGTGGCGACGATCTC
GGCATCGCCGGAAATCCTCACCGCGCTCCGGTCCGCCCTGCGCAACCGCATGCAGGCCGTGGTCAACATCGCCATGGGCG
CCTCGCTGTCGACGGTGATCCTGACCGTGCCGGTCATGGAGGGCATCGCGCTTTATACCGGCCAGCCCTTCATCATGGCG
ATGACGCCGGTTCAGACCGTGATGGTCTTCATCACCCTCATCGCTGCGGCCATCAACCTCAACGACGGTGAAACCAATGC
GATCGAGGGAATGACACATTTCATTCTCTTCGCCACCTTCATCATGCTGCTGTTTCTCGGGCTCTGA

Upstream 100 bases:

>100_bases
TGCGACATCACTATCCTACCAAAAGCATTCAAGCGCAAATCCTGCTTGATCTGAGGGACGATACGTCACAGCCTAAGGCC
TGTCAGGGGAAGGGAAATCG

Downstream 100 bases:

>100_bases
CCGAAACGAAAAAAGCCCGGCATCGCTGCCGGGCTTCTTTATACAATATAGCGGGCCGATCAGCGGCAATCGGCGCAGAA
ACGCTGGATGCGCTTGCAGG

Product: Ca2+/H+ antiporter

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MVGSILKQERMLLLAIPAAIAAYMVEHAIFEAGKTAGLIAAIVLIGLIVLVSMRVAHHAEILAAKVGDPYGTMILTLSAV
AVEVLILAIIMSESSSPTLVRDTIYSAVMIDINGILGLAALIGGLRHGEQPYNDDSGKTYGVMILTAMGISMIVPEFIPQ
QSWHIYSVFTIGAMIALYALFLKMQVGPHSYFFSYTYPRKAHPVGQASDGHGQEEDEGSVPLSIGLIIAGVVLIGVLAEF
MSAFLGESLEGTSAPPALMAVVVATISASPEILTALRSALRNRMQAVVNIAMGASLSTVILTVPVMEGIALYTGQPFIMA
MTPVQTVMVFITLIAAAINLNDGETNAIEGMTHFILFATFIMLLFLGL

Sequences:

>Translated_368_residues
MVGSILKQERMLLLAIPAAIAAYMVEHAIFEAGKTAGLIAAIVLIGLIVLVSMRVAHHAEILAAKVGDPYGTMILTLSAV
AVEVLILAIIMSESSSPTLVRDTIYSAVMIDINGILGLAALIGGLRHGEQPYNDDSGKTYGVMILTAMGISMIVPEFIPQ
QSWHIYSVFTIGAMIALYALFLKMQVGPHSYFFSYTYPRKAHPVGQASDGHGQEEDEGSVPLSIGLIIAGVVLIGVLAEF
MSAFLGESLEGTSAPPALMAVVVATISASPEILTALRSALRNRMQAVVNIAMGASLSTVILTVPVMEGIALYTGQPFIMA
MTPVQTVMVFITLIAAAINLNDGETNAIEGMTHFILFATFIMLLFLGL
>Mature_368_residues
MVGSILKQERMLLLAIPAAIAAYMVEHAIFEAGKTAGLIAAIVLIGLIVLVSMRVAHHAEILAAKVGDPYGTMILTLSAV
AVEVLILAIIMSESSSPTLVRDTIYSAVMIDINGILGLAALIGGLRHGEQPYNDDSGKTYGVMILTAMGISMIVPEFIPQ
QSWHIYSVFTIGAMIALYALFLKMQVGPHSYFFSYTYPRKAHPVGQASDGHGQEEDEGSVPLSIGLIIAGVVLIGVLAEF
MSAFLGESLEGTSAPPALMAVVVATISASPEILTALRSALRNRMQAVVNIAMGASLSTVILTVPVMEGIALYTGQPFIMA
MTPVQTVMVFITLIAAAINLNDGETNAIEGMTHFILFATFIMLLFLGL

Specific function: According to PubMed:8496184 the natural activity of this protein is as a calcium/proton antiporter, but it can act as a weak sodium/proton antiporter. The presence of a preferred substrate and normal pH cytoplasmic values might prevent significant sodium/

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1787468, Length=341, Percent_Identity=31.6715542521994, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 38981; Mature: 38981

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGSILKQERMLLLAIPAAIAAYMVEHAIFEAGKTAGLIAAIVLIGLIVLVSMRVAHHAE
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILAAKVGDPYGTMILTLSAVAVEVLILAIIMSESSSPTLVRDTIYSAVMIDINGILGLAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIGGLRHGEQPYNDDSGKTYGVMILTAMGISMIVPEFIPQQSWHIYSVFTIGAMIALYAL
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
FLKMQVGPHSYFFSYTYPRKAHPVGQASDGHGQEEDEGSVPLSIGLIIAGVVLIGVLAEF
HHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MSAFLGESLEGTSAPPALMAVVVATISASPEILTALRSALRNRMQAVVNIAMGASLSTVI
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LTVPVMEGIALYTGQPFIMAMTPVQTVMVFITLIAAAINLNDGETNAIEGMTHFILFATF
HHHHHHHCHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
IMLLFLGL
HHHHHHCC
>Mature Secondary Structure
MVGSILKQERMLLLAIPAAIAAYMVEHAIFEAGKTAGLIAAIVLIGLIVLVSMRVAHHAE
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILAAKVGDPYGTMILTLSAVAVEVLILAIIMSESSSPTLVRDTIYSAVMIDINGILGLAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIGGLRHGEQPYNDDSGKTYGVMILTAMGISMIVPEFIPQQSWHIYSVFTIGAMIALYAL
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
FLKMQVGPHSYFFSYTYPRKAHPVGQASDGHGQEEDEGSVPLSIGLIIAGVVLIGVLAEF
HHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MSAFLGESLEGTSAPPALMAVVVATISASPEILTALRSALRNRMQAVVNIAMGASLSTVI
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LTVPVMEGIALYTGQPFIMAMTPVQTVMVFITLIAAAINLNDGETNAIEGMTHFILFATF
HHHHHHHCHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
IMLLFLGL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8496184; 8905232; 9278503 [H]