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The map label for this gene is polC [H]

Identifier: 15889361

GI number: 15889361

Start: 2036440

End: 2037048

Strand: Reverse

Name: polC [H]

Synonym: Atu2077

Alternate gene names: 15889361

Gene position: 2037048-2036440 (Counterclockwise)

Preceding gene: 15889362

Following gene: 15889360

Centisome position: 71.69

GC content: 58.29

Gene sequence:

>609_bases
TTGAAAACTATCGCCATCGATTTCGAGACCGCCAATGAGGAGCGGGGCAGCGCCTGCTCCGTCGGGCTTGCGTGGATTGA
GGACGGCCAGATCGTTCGTGTCGAGGAACGTCTCATCCGCCCCAGGGACATGCGGTTTTCGCCCTTCAACATCGCGGTTC
ACGGAATTCGCCCTCGTGATGTCGAGGACGCGCCGGAGTTTCCTGAGGTGATGGAGGAGTTCATCGACGATTTCCACGGC
GCGACCATGATCGCTCATAATGCCGCCTTCGATTTCAGCGTCATGCGTGCGTCGTTCGACAAGTACCGGCTGTCCTATCC
TGATCTTTCCTACCTCTGCAGCGTCAAGATCGCCCGACATGTCTGGCCACATCTGGTGTCGCACAAGCTGAATGTCATTG
CCCATCATCTCCAGTTGCGTTTCGTGCATCATAATGCGGCGGAAGATGCGGTTGTCTGTGCTGCCGCCTCGATTGCGGCG
GCGCGGGCTCTCGGTGTTGCGCATATTCGCGATCTGCCGGAAAAGATCGGTATGGTCGCTGGCCGTCTGACGTCCACGGG
TTATCAGCCCTGCAGCATGAAAAAACGGACCGTCGCACGGGTTGCCTGA

Upstream 100 bases:

>100_bases
ACGCTCATCGACTGGATGCTTTCGCAAGCGGGTTCCACTGAGGCTGGTTCTCTTTGACGCCAATCCGGTCTATGCCGGAA
GACAACAACGGGAATCACTC

Downstream 100 bases:

>100_bases
GCGGGTTTTCCACCTATGTCTTATGCAAGCGTAACGGAGAAGATGCGATGAGGATGAAAACTCTGTCGGGTTTGTTTGCG
GGCCTTCTGGCATTGCTGCC

Product: DNA polymerase III, epsilon subunit

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MKTIAIDFETANEERGSACSVGLAWIEDGQIVRVEERLIRPRDMRFSPFNIAVHGIRPRDVEDAPEFPEVMEEFIDDFHG
ATMIAHNAAFDFSVMRASFDKYRLSYPDLSYLCSVKIARHVWPHLVSHKLNVIAHHLQLRFVHHNAAEDAVVCAAASIAA
ARALGVAHIRDLPEKIGMVAGRLTSTGYQPCSMKKRTVARVA

Sequences:

>Translated_202_residues
MKTIAIDFETANEERGSACSVGLAWIEDGQIVRVEERLIRPRDMRFSPFNIAVHGIRPRDVEDAPEFPEVMEEFIDDFHG
ATMIAHNAAFDFSVMRASFDKYRLSYPDLSYLCSVKIARHVWPHLVSHKLNVIAHHLQLRFVHHNAAEDAVVCAAASIAA
ARALGVAHIRDLPEKIGMVAGRLTSTGYQPCSMKKRTVARVA
>Mature_202_residues
MKTIAIDFETANEERGSACSVGLAWIEDGQIVRVEERLIRPRDMRFSPFNIAVHGIRPRDVEDAPEFPEVMEEFIDDFHG
ATMIAHNAAFDFSVMRASFDKYRLSYPDLSYLCSVKIARHVWPHLVSHKLNVIAHHLQLRFVHHNAAEDAVVCAAASIAA
ARALGVAHIRDLPEKIGMVAGRLTSTGYQPCSMKKRTVARVA

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1786409, Length=134, Percent_Identity=32.089552238806, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR023223
- InterPro:   IPR016027
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 22606; Mature: 22606

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIAIDFETANEERGSACSVGLAWIEDGQIVRVEERLIRPRDMRFSPFNIAVHGIRPRD
CCEEEEEEECCCCCCCCEEEEEEEEECCCCEEEEHHHHCCCCCCCCCCCEEEEECCCCCC
VEDAPEFPEVMEEFIDDFHGATMIAHNAAFDFSVMRASFDKYRLSYPDLSYLCSVKIARH
CCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VWPHLVSHKLNVIAHHLQLRFVHHNAAEDAVVCAAASIAAARALGVAHIRDLPEKIGMVA
HHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRLTSTGYQPCSMKKRTVARVA
HHHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MKTIAIDFETANEERGSACSVGLAWIEDGQIVRVEERLIRPRDMRFSPFNIAVHGIRPRD
CCEEEEEEECCCCCCCCEEEEEEEEECCCCEEEEHHHHCCCCCCCCCCCEEEEECCCCCC
VEDAPEFPEVMEEFIDDFHGATMIAHNAAFDFSVMRASFDKYRLSYPDLSYLCSVKIARH
CCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VWPHLVSHKLNVIAHHLQLRFVHHNAAEDAVVCAAASIAAARALGVAHIRDLPEKIGMVA
HHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRLTSTGYQPCSMKKRTVARVA
HHHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]