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The map label for this gene is rlmB [H]

Identifier: 15889284

GI number: 15889284

Start: 1957166

End: 1958053

Strand: Direct

Name: rlmB [H]

Synonym: Atu1993

Alternate gene names: 15889284

Gene position: 1957166-1958053 (Clockwise)

Preceding gene: 15889283

Following gene: 15889285

Centisome position: 68.88

GC content: 62.16

Gene sequence:

>888_bases
ATGAGCAAAGACGATCCAAACGACAAATCCACCCGCGATACGCACTACGCCACACTGCGCCGTGCCGTACGCGATGCCAA
ACGCGAGCGCGGCGAAATTCCGACCCCGCAGCAGCCGAAGCGCCGAAATCGTGGCGCGGACGACTGGAAGCCGCCGCAGC
TTGCGCCGGATCAGGTCCATCTTTACGGCCTGCATACCGTGCGCGCGGCGCTTTCCAATCCCCAACGGCAGCTCATCAAG
CTGTCGGTGACACTCAATGCCTTTGCGCGTCTGGATATCGGCGAGGCGGATGCACAGCCGTTCCCTGTCGAAATCGTCTC
GCCGCAGGAGATCGACAAGGTGCTCGGCCCCGAGGCGATCCATCAGGGCGTGATGCTGGAGACCAGGCCGCTTCCGGTCA
AGAAGCTCGACGCCTTGAAGAAAAGCCCGCTGATCCTCGTGCTCGATCAGGTGACCGATCCGCACAATGTCGGCGCCATC
ATGCGCTCGGCGGTCGCGTTCAACGCCGGCGCCGTCATCACCACCATCCGCCATAGCCCGACGGAGTCAGGCGTGCTGGC
GAAATCCGCGTCCGGTGCACTGGAACTCATTCCCTATATCCAGATTACCAACCTCGCCGACACATTGGGCGAGCTGCACA
AGCTCGGCTTCTTCTCGGTCGGTCTGGATTCGGAAGGCCCCGCTCCCATCGAGCAAACCTTCACCGGTGCAAAGATTGCC
CTGGTGCTAGGCGCGGAAGGCAAGGGCCTGCGCCAGAAGACCCGCGAAACCGTTTCGGCGCTCGCCCGTCTCGACATGCC
GGGCGAGATCAAATCGCTCAACGTATCGAATGCGGCGGCCATCGCCCTTTATGCAACGCAGCAATTTCTGGGCAAAGCGT
CGTCCTGA

Upstream 100 bases:

>100_bases
GCCGTTTCACGAATTTTCGGGAAAAAATTCAATCCGCTTTGCAGCCCTGTGGATTATCGCGGGGGCTGGTGTGCGTCGCG
TCGCAGAGATATAAGGCGCC

Downstream 100 bases:

>100_bases
CGCTTGCCCTTCCTTGCGAAATAACAAAGCGCAACGCATGCTGCGTCTTTAACGTTCGCAAGGAGCAAATCCCGACATGA
CCGACCTGCCCATCAAACCG

Product: tRNA/rRNA metyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSKDDPNDKSTRDTHYATLRRAVRDAKRERGEIPTPQQPKRRNRGADDWKPPQLAPDQVHLYGLHTVRAALSNPQRQLIK
LSVTLNAFARLDIGEADAQPFPVEIVSPQEIDKVLGPEAIHQGVMLETRPLPVKKLDALKKSPLILVLDQVTDPHNVGAI
MRSAVAFNAGAVITTIRHSPTESGVLAKSASGALELIPYIQITNLADTLGELHKLGFFSVGLDSEGPAPIEQTFTGAKIA
LVLGAEGKGLRQKTRETVSALARLDMPGEIKSLNVSNAAAIALYATQQFLGKASS

Sequences:

>Translated_295_residues
MSKDDPNDKSTRDTHYATLRRAVRDAKRERGEIPTPQQPKRRNRGADDWKPPQLAPDQVHLYGLHTVRAALSNPQRQLIK
LSVTLNAFARLDIGEADAQPFPVEIVSPQEIDKVLGPEAIHQGVMLETRPLPVKKLDALKKSPLILVLDQVTDPHNVGAI
MRSAVAFNAGAVITTIRHSPTESGVLAKSASGALELIPYIQITNLADTLGELHKLGFFSVGLDSEGPAPIEQTFTGAKIA
LVLGAEGKGLRQKTRETVSALARLDMPGEIKSLNVSNAAAIALYATQQFLGKASS
>Mature_294_residues
SKDDPNDKSTRDTHYATLRRAVRDAKRERGEIPTPQQPKRRNRGADDWKPPQLAPDQVHLYGLHTVRAALSNPQRQLIKL
SVTLNAFARLDIGEADAQPFPVEIVSPQEIDKVLGPEAIHQGVMLETRPLPVKKLDALKKSPLILVLDQVTDPHNVGAIM
RSAVAFNAGAVITTIRHSPTESGVLAKSASGALELIPYIQITNLADTLGELHKLGFFSVGLDSEGPAPIEQTFTGAKIAL
VLGAEGKGLRQKTRETVSALARLDMPGEIKSLNVSNAAAIALYATQQFLGKASS

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=212, Percent_Identity=33.0188679245283, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1790623, Length=235, Percent_Identity=35.3191489361702, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788935, Length=140, Percent_Identity=30.7142857142857, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=283, Percent_Identity=25.0883392226148, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31859; Mature: 31728

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDDPNDKSTRDTHYATLRRAVRDAKRERGEIPTPQQPKRRNRGADDWKPPQLAPDQVH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCEE
LYGLHTVRAALSNPQRQLIKLSVTLNAFARLDIGEADAQPFPVEIVSPQEIDKVLGPEAI
EEHHHHHHHHHCCCCCEEEEEEEEEEEHEEECCCCCCCCCCCEEEECHHHHHHHCCHHHH
HQGVMLETRPLPVKKLDALKKSPLILVLDQVTDPHNVGAIMRSAVAFNAGAVITTIRHSP
HCCCEEECCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECCC
TESGVLAKSASGALELIPYIQITNLADTLGELHKLGFFSVGLDSEGPAPIEQTFTGAKIA
CCCCEEEECCCCCEEEECCEEEHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHCCCCEEE
LVLGAEGKGLRQKTRETVSALARLDMPGEIKSLNVSNAAAIALYATQQFLGKASS
EEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEHHHHHHHHCCCCC
>Mature Secondary Structure 
SKDDPNDKSTRDTHYATLRRAVRDAKRERGEIPTPQQPKRRNRGADDWKPPQLAPDQVH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCEE
LYGLHTVRAALSNPQRQLIKLSVTLNAFARLDIGEADAQPFPVEIVSPQEIDKVLGPEAI
EEHHHHHHHHHCCCCCEEEEEEEEEEEHEEECCCCCCCCCCCEEEECHHHHHHHCCHHHH
HQGVMLETRPLPVKKLDALKKSPLILVLDQVTDPHNVGAIMRSAVAFNAGAVITTIRHSP
HCCCEEECCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECCC
TESGVLAKSASGALELIPYIQITNLADTLGELHKLGFFSVGLDSEGPAPIEQTFTGAKIA
CCCCEEEECCCCCEEEECCEEEHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHCCCCEEE
LVLGAEGKGLRQKTRETVSALARLDMPGEIKSLNVSNAAAIALYATQQFLGKASS
EEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]