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The map label for this gene is artJ [H]

Identifier: 15889178

GI number: 15889178

Start: 1860027

End: 1860800

Strand: Direct

Name: artJ [H]

Synonym: Atu1879

Alternate gene names: 15889178

Gene position: 1860027-1860800 (Clockwise)

Preceding gene: 159184961

Following gene: 15889179

Centisome position: 65.46

GC content: 58.79

Gene sequence:

>774_bases
ATGCCTATTTCCACCCGTCTGCTCGCAGCCGTATCGATTGCCGCCATCTCGCTGTTGTCAGGCGCAGCCCTTGCGCAGGA
CAAGATCGTCATCGGCACCGAAGGCGCCTACCCGCCCTTCAACAATCTCGAAGCCGACGGCACGCTGACCGGCTTCGACA
TCGACATCGCCAAGGCGCTGTGCGAACAAATGAAGGCCGAATGCACCTTCGTCACCAATGACTGGGACGGCATCATTCCC
GCCCTGCAGGCCAAGAAGTTCGACGCCATCATCGCGTCCATGTCGATCACGCCGGAACGCCTCCAGAAGGTCGATTTCTC
CAAGAAATATTACAACACCCCGCCGGCAATCGCCGTGCCGAAGGATTCGCCGATCAAGTCCATCGACGATCTCAAGGGCA
AGTCGCTCGGCGCGCAGGGTTCCACCACACACTCCAACTATGCCGAAAAGCATTTCCCGGATGCCGACGTGAAGATGTAT
CCGACCGCCGACGAATATAAGCTCGACATCGCCAATGGCCGCATCGACGCCGTCATCGACGATATCGTCGTGCTGTCGGA
GTGGCTGAAGACCGACGCCGGCAAGTGCTGCAAGATCCTGACGCCGCTCAAGGTCGATCCCGAGATCAACGGCAATGGCG
CAGGCATTGCCGTGCGCAAGGGCGACACGGCGCTGGCCGACAAGTTCACGGCCGCAATCGCCGGTATCCGCGCCAGCGGC
AAGTATCAGGAGATCAACAAAAAATACTTCGATTTCGACGTTTACGGCGATTAA

Upstream 100 bases:

>100_bases
CCACAGGCGGTATCCTCCGTTCTGCCGCGCCCAGCGCCCATGGCATCCGCACTGAAAATAACAACAGACTGCGGATGAAC
GACCAACAGAGAGGATCGAA

Downstream 100 bases:

>100_bases
GCCGGTTCGATCTGTTGAAATCTCGTGGCGGAAGGCTTGCCCTTCCGCCATTTTTGTTTGAAAACGTCACTATAACAATC
ACGGCGGCTTAAGACCGCAG

Product: ABC transporter, substrate binding protein (amino acid)

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MPISTRLLAAVSIAAISLLSGAALAQDKIVIGTEGAYPPFNNLEADGTLTGFDIDIAKALCEQMKAECTFVTNDWDGIIP
ALQAKKFDAIIASMSITPERLQKVDFSKKYYNTPPAIAVPKDSPIKSIDDLKGKSLGAQGSTTHSNYAEKHFPDADVKMY
PTADEYKLDIANGRIDAVIDDIVVLSEWLKTDAGKCCKILTPLKVDPEINGNGAGIAVRKGDTALADKFTAAIAGIRASG
KYQEINKKYFDFDVYGD

Sequences:

>Translated_257_residues
MPISTRLLAAVSIAAISLLSGAALAQDKIVIGTEGAYPPFNNLEADGTLTGFDIDIAKALCEQMKAECTFVTNDWDGIIP
ALQAKKFDAIIASMSITPERLQKVDFSKKYYNTPPAIAVPKDSPIKSIDDLKGKSLGAQGSTTHSNYAEKHFPDADVKMY
PTADEYKLDIANGRIDAVIDDIVVLSEWLKTDAGKCCKILTPLKVDPEINGNGAGIAVRKGDTALADKFTAAIAGIRASG
KYQEINKKYFDFDVYGD
>Mature_256_residues
PISTRLLAAVSIAAISLLSGAALAQDKIVIGTEGAYPPFNNLEADGTLTGFDIDIAKALCEQMKAECTFVTNDWDGIIPA
LQAKKFDAIIASMSITPERLQKVDFSKKYYNTPPAIAVPKDSPIKSIDDLKGKSLGAQGSTTHSNYAEKHFPDADVKMYP
TADEYKLDIANGRIDAVIDDIVVLSEWLKTDAGKCCKILTPLKVDPEINGNGAGIAVRKGDTALADKFTAAIAGIRASGK
YQEINKKYFDFDVYGD

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=40.4081632653061, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=40.0778210116732, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=38.3673469387755, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=41.3793103448276, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787031, Length=243, Percent_Identity=32.0987654320988, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788228, Length=229, Percent_Identity=31.8777292576419, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27643; Mature: 27512

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISTRLLAAVSIAAISLLSGAALAQDKIVIGTEGAYPPFNNLEADGTLTGFDIDIAKAL
CCCCHHHHHHHHHHHHHHHHCCHHCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHH
CEQMKAECTFVTNDWDGIIPALQAKKFDAIIASMSITPERLQKVDFSKKYYNTPPAIAVP
HHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHCCCCCCEECC
KDSPIKSIDDLKGKSLGAQGSTTHSNYAEKHFPDADVKMYPTADEYKLDIANGRIDAVID
CCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCCEEEEECCCCHHHHHH
DIVVLSEWLKTDAGKCCKILTPLKVDPEINGNGAGIAVRKGDTALADKFTAAIAGIRASG
HHHHHHHHHHCCCCCCEEECCCCEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCC
KYQEINKKYFDFDVYGD
CHHHHCCEEEEEEECCC
>Mature Secondary Structure 
PISTRLLAAVSIAAISLLSGAALAQDKIVIGTEGAYPPFNNLEADGTLTGFDIDIAKAL
CCCHHHHHHHHHHHHHHHHCCHHCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHH
CEQMKAECTFVTNDWDGIIPALQAKKFDAIIASMSITPERLQKVDFSKKYYNTPPAIAVP
HHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHCCCCCCEECC
KDSPIKSIDDLKGKSLGAQGSTTHSNYAEKHFPDADVKMYPTADEYKLDIANGRIDAVID
CCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCCEEEEECCCCHHHHHH
DIVVLSEWLKTDAGKCCKILTPLKVDPEINGNGAGIAVRKGDTALADKFTAAIAGIRASG
HHHHHHHHHHCCCCCCEEECCCCEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCC
KYQEINKKYFDFDVYGD
CHHHHCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]