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The map label for this gene is metS

Identifier: 15888821

GI number: 15888821

Start: 1485208

End: 1486758

Strand: Reverse

Name: metS

Synonym: Atu1496

Alternate gene names: 15888821

Gene position: 1486758-1485208 (Counterclockwise)

Preceding gene: 159184783

Following gene: 159184782

Centisome position: 52.32

GC content: 60.22

Gene sequence:

>1551_bases
ATGACAGACAAGACACCGTTCTACATCACCACCGCGATCTCCTACCCCAACGGCAAGCCGCATATCGGCCATGCCTATGA
GTTGATTGCGACGGACGCCATGGCACGTTTTCAGCGCCTGGACGGAATGGATGTTTTCTTCCTGACCGGCACGGACGAAC
ACGGCCAGAAGATGCAGCAGACGGCAAGGGCGGAAGGCATTTCGCCGGAAGAGCTGGCGCAGCGCAATTCCGACCAGTTC
CGTGAAATGGGCAAGCTGCTCAACGCCTCGAACGACGATTTCATCCGCACCACGGAAGAGCGTCACCACGAGACCTCGCG
CAATATCTGGAACCTGATGGCCGATAGCGGCGACATCTACAAGGACAGCTATGCCGGCTGGTATTCCGTGCGCGACGAGG
CCTATTACGCCGAGGACGAGACGGAAGTGCGCGCAGACGGTGTCCGTTACGGGCCGCAGGGCACACCGGTCGAATGGGTG
GAAGAGGAAAGCTACTTCTTCAAGCTCTCCGAATATCAGGAAAAACTCCTGAAGCTCTACGAGGAGAACCCTGACTTCAT
CGGTCCGGCCGAGCGCCGCAACGAGATCATCTCGTTCGTCAAATCCGGTCTGAAGGACCTGTCGATTTCCCGCACCACCT
TTGACTGGGGCATCAAGGTTCCCGACGATCCGAAGCACGTCATGTATGTCTGGGTCGATGCGCTGACCAACTACATTACC
GCGACCGGCTACATCGAGGACAGGAACGGCCCGCGCGCCAAATATTGGCCTGCCGACGTGCACATCATCGGCAAGGACAT
CATCCGCTTCCACGCGGTCTACTGGCCGGCTTTCCTGATGAGCGCCAAGCTGCCGCTGCCCAAGCGGGTCTACGCGCACG
GCTTCCTGCTCAACAAGGGCGAGAAGATGTCGAAGTCGCTCGGCAATGTCGTCGACCCTGCCAATCTGGTTGCCCATTTC
GGCCTCGATCCGGTTCGTTATTTCTTCATGCGCGAAGTCTCTTTCGGCCAGGATGGCAGCTATAGCGAAGAGGGCATCGC
CACCCGCATCAATGCCGATCTCGCCAATGGCATCGGCAATCTCGCCAGCCGTTCGCTGTCGATGATCGTCAAGAATTGCG
ACGGGCAGATCCCGACGCCCGGCGCATTCAACGATGACGACAAAGCGATGCTCGCTTCTGCCGACGGGCTGCTTGCGGTC
TGCCGCGAGGAAATGGGCAAGCAGCTCATTCACCGCGCGCTTGCCGCCATCATCGCCGTCGTTTCGGAAACCGACCGTTA
TTTCGCGTCGCAGGAACCGTGGGCGCTGAAGAAGACCGATCCGGAGCGCATGGGCACCGTGCTTTACGTGACGGCCGAAG
TGGTGCGCCAGATCGGCATCCTGCTGCAGCCCTTCATGCCTCAGTCCTGCGAGAAGCTGCTCGATCTCGTTGCGGCGCCT
GCCGACGGCAGAGATTTCACGGCGCTCGGCGAAGCCGGTCGTCTGGCTCCAGGCACACCGCTCGAAGCGCCGAAGCCGGT
CTTCCCGCGTTACGTTGCCCCGGAAGCGTGA

Upstream 100 bases:

>100_bases
TGAATCATACCCAAAATGGGGAAGTTGTTGTTTCGCTGCTGCGAACAATGGTTTAAGAGCGGTCTATCCAAAAAACAGCC
TGTAAAGTCGATAGACCGTC

Downstream 100 bases:

>100_bases
AGGCGAGACCATGCTGATCGATACCCATTGCCATCTGGATTTTCCCGATTTCGAGGCGGAGCGCGACGATATCATCGCCC
GTGCTCACGCCTCGGGTGTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTDKTPFYITTAISYPNGKPHIGHAYELIATDAMARFQRLDGMDVFFLTGTDEHGQKMQQTARAEGISPEELAQRNSDQF
REMGKLLNASNDDFIRTTEERHHETSRNIWNLMADSGDIYKDSYAGWYSVRDEAYYAEDETEVRADGVRYGPQGTPVEWV
EEESYFFKLSEYQEKLLKLYEENPDFIGPAERRNEIISFVKSGLKDLSISRTTFDWGIKVPDDPKHVMYVWVDALTNYIT
ATGYIEDRNGPRAKYWPADVHIIGKDIIRFHAVYWPAFLMSAKLPLPKRVYAHGFLLNKGEKMSKSLGNVVDPANLVAHF
GLDPVRYFFMREVSFGQDGSYSEEGIATRINADLANGIGNLASRSLSMIVKNCDGQIPTPGAFNDDDKAMLASADGLLAV
CREEMGKQLIHRALAAIIAVVSETDRYFASQEPWALKKTDPERMGTVLYVTAEVVRQIGILLQPFMPQSCEKLLDLVAAP
ADGRDFTALGEAGRLAPGTPLEAPKPVFPRYVAPEA

Sequences:

>Translated_516_residues
MTDKTPFYITTAISYPNGKPHIGHAYELIATDAMARFQRLDGMDVFFLTGTDEHGQKMQQTARAEGISPEELAQRNSDQF
REMGKLLNASNDDFIRTTEERHHETSRNIWNLMADSGDIYKDSYAGWYSVRDEAYYAEDETEVRADGVRYGPQGTPVEWV
EEESYFFKLSEYQEKLLKLYEENPDFIGPAERRNEIISFVKSGLKDLSISRTTFDWGIKVPDDPKHVMYVWVDALTNYIT
ATGYIEDRNGPRAKYWPADVHIIGKDIIRFHAVYWPAFLMSAKLPLPKRVYAHGFLLNKGEKMSKSLGNVVDPANLVAHF
GLDPVRYFFMREVSFGQDGSYSEEGIATRINADLANGIGNLASRSLSMIVKNCDGQIPTPGAFNDDDKAMLASADGLLAV
CREEMGKQLIHRALAAIIAVVSETDRYFASQEPWALKKTDPERMGTVLYVTAEVVRQIGILLQPFMPQSCEKLLDLVAAP
ADGRDFTALGEAGRLAPGTPLEAPKPVFPRYVAPEA
>Mature_515_residues
TDKTPFYITTAISYPNGKPHIGHAYELIATDAMARFQRLDGMDVFFLTGTDEHGQKMQQTARAEGISPEELAQRNSDQFR
EMGKLLNASNDDFIRTTEERHHETSRNIWNLMADSGDIYKDSYAGWYSVRDEAYYAEDETEVRADGVRYGPQGTPVEWVE
EESYFFKLSEYQEKLLKLYEENPDFIGPAERRNEIISFVKSGLKDLSISRTTFDWGIKVPDDPKHVMYVWVDALTNYITA
TGYIEDRNGPRAKYWPADVHIIGKDIIRFHAVYWPAFLMSAKLPLPKRVYAHGFLLNKGEKMSKSLGNVVDPANLVAHFG
LDPVRYFFMREVSFGQDGSYSEEGIATRINADLANGIGNLASRSLSMIVKNCDGQIPTPGAFNDDDKAMLASADGLLAVC
REEMGKQLIHRALAAIIAVVSETDRYFASQEPWALKKTDPERMGTVLYVTAEVVRQIGILLQPFMPQSCEKLLDLVAAPA
DGRDFTALGEAGRLAPGTPLEAPKPVFPRYVAPEA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily

Homologues:

Organism=Homo sapiens, GI24308436, Length=542, Percent_Identity=35.6088560885609, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI14043022, Length=505, Percent_Identity=24.950495049505, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1788432, Length=503, Percent_Identity=26.6401590457256, Blast_Score=188, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI212645096, Length=498, Percent_Identity=35.5421686746988, Blast_Score=322, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI71992781, Length=387, Percent_Identity=34.8837209302326, Blast_Score=244, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI17540794, Length=523, Percent_Identity=25.4302103250478, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321610, Length=531, Percent_Identity=31.4500941619586, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6321704, Length=514, Percent_Identity=22.1789883268482, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21358483, Length=511, Percent_Identity=35.6164383561644, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24655311, Length=548, Percent_Identity=25.5474452554745, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21355409, Length=179, Percent_Identity=26.8156424581006, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_AGRT5 (Q8UFA2)

Other databases:

- EMBL:   AE007869
- PIR:   AH2760
- PIR:   F97541
- RefSeq:   NP_354502.1
- ProteinModelPortal:   Q8UFA2
- SMR:   Q8UFA2
- STRING:   Q8UFA2
- GeneID:   1133534
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1496
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- PhylomeDB:   Q8UFA2
- ProtClustDB:   PRK11893
- BioCyc:   ATUM176299-1:ATU1496-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 57931; Mature: 57800

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 305-305

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKTPFYITTAISYPNGKPHIGHAYELIATDAMARFQRLDGMDVFFLTGTDEHGQKMQQ
CCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
TARAEGISPEELAQRNSDQFREMGKLLNASNDDFIRTTEERHHETSRNIWNLMADSGDIY
HHHHCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCC
KDSYAGWYSVRDEAYYAEDETEVRADGVRYGPQGTPVEWVEEESYFFKLSEYQEKLLKLY
CCCCCCEEEECCCEEECCCCCHHHHCCEEECCCCCCHHHCCCCHHEEEHHHHHHHHHHHH
EENPDFIGPAERRNEIISFVKSGLKDLSISRTTFDWGIKVPDDPKHVMYVWVDALTNYIT
HCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEHHHHHHHHE
ATGYIEDRNGPRAKYWPADVHIIGKDIIRFHAVYWPAFLMSAKLPLPKRVYAHGFLLNKG
EEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEECCC
EKMSKSLGNVVDPANLVAHFGLDPVRYFFMREVSFGQDGSYSEEGIATRINADLANGIGN
HHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHH
LASRSLSMIVKNCDGQIPTPGAFNDDDKAMLASADGLLAVCREEMGKQLIHRALAAIIAV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VSETDRYFASQEPWALKKTDPERMGTVLYVTAEVVRQIGILLQPFMPQSCEKLLDLVAAP
HHHHHHHHCCCCCCCCCCCCHHHHCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
ADGRDFTALGEAGRLAPGTPLEAPKPVFPRYVAPEA
CCCCCEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDKTPFYITTAISYPNGKPHIGHAYELIATDAMARFQRLDGMDVFFLTGTDEHGQKMQQ
CCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
TARAEGISPEELAQRNSDQFREMGKLLNASNDDFIRTTEERHHETSRNIWNLMADSGDIY
HHHHCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCC
KDSYAGWYSVRDEAYYAEDETEVRADGVRYGPQGTPVEWVEEESYFFKLSEYQEKLLKLY
CCCCCCEEEECCCEEECCCCCHHHHCCEEECCCCCCHHHCCCCHHEEEHHHHHHHHHHHH
EENPDFIGPAERRNEIISFVKSGLKDLSISRTTFDWGIKVPDDPKHVMYVWVDALTNYIT
HCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEHHHHHHHHE
ATGYIEDRNGPRAKYWPADVHIIGKDIIRFHAVYWPAFLMSAKLPLPKRVYAHGFLLNKG
EEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEECCC
EKMSKSLGNVVDPANLVAHFGLDPVRYFFMREVSFGQDGSYSEEGIATRINADLANGIGN
HHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHH
LASRSLSMIVKNCDGQIPTPGAFNDDDKAMLASADGLLAVCREEMGKQLIHRALAAIIAV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VSETDRYFASQEPWALKKTDPERMGTVLYVTAEVVRQIGILLQPFMPQSCEKLLDLVAAP
HHHHHHHHCCCCCCCCCCCCHHHHCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
ADGRDFTALGEAGRLAPGTPLEAPKPVFPRYVAPEA
CCCCCEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194