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The map label for this gene is lipA [H]

Identifier: 15888761

GI number: 15888761

Start: 1430402

End: 1431373

Strand: Direct

Name: lipA [H]

Synonym: Atu1436

Alternate gene names: 15888761

Gene position: 1430402-1431373 (Clockwise)

Preceding gene: 159184759

Following gene: 15888765

Centisome position: 50.34

GC content: 59.67

Gene sequence:

>972_bases
ATGGTCACTATTCTCGACAGGACGTCGTCCGACGAAAAGCGCATCCGCCACCCGGAAAAGGCGCATCGGCCCGATACCGA
GGTCATGCGCAAGCCGGAATGGATCCGCGTCAAGGCGCCGACTTCCAAGGGTTATCACGAGACGCGCGAACTGGTGCGCA
GCCACAAGCTGGTAACGGTGTGCGAGGAAGCCGGCTGTCCGAATATCGGCGAGTGCTGGGAAAAGAAACACGCGACCTTC
ATGATCATGGGTGAAATCTGCACCCGTGCCTGCGCCTTCTGTAACGTCGCGACCGGCAAGCCCAATGCGCTTGACATGGA
CGAGCCCGAAAACGTCGCCAAGGCCGTCAAGCAGATGGGCCTTTCACATGTCGTTATCACCTCGGTTGACCGTGACGATC
TCGCCGACGGCGGTGCTGAACATTTCGAGAAGGTCATCTGGGCGATCCGTGCCGCGTCTCCGACGACGACCATCGAAATC
CTCACCCCCGACTTCCTGAAGAAGCCCGGCGCGCTGGAGCGCGTCGTCGCCGCCAAGCCTGATGTCTTCAACCACAACAT
GGAAACTGTTCCGGGCAATTACCTCACGGTTCGTCCGGGCGCGCGTTATTTCCATTCCGTGCGTCTCTTGCAGCGTGTCA
AGGAACTGGACCCCACCATGTTCACCAAGTCCGGCATCATGGTCGGCTTGGGTGAAGAGCGCAACGAAGTGCTGCAGCTG
ATGGACGATCTGCGCAGCGCCGACGTGGATTTCCTGACCATCGGCCAATATCTGCAGCCGACCCGCAAACACCACAAGGT
TGAGGCCTTCGTCACACCGGAAGAATTCAAGTCCTACGAGACTGTGGCCTATGCCAAGGGCTTCCTGATGGTCTCTTCCA
GCCCGCTGACGCGTTCTTCCCACCATGCCGGCGACGATTTTGAGCGCCTGCGTGCGGCCCGCGAGAAGAAGCTGCTGGCT
GCTGCTGAGTAA

Upstream 100 bases:

>100_bases
GCCGCATGACCGGAAGGGGCTTACGCTTTTTCTGGAATTGTTCTAAAGCATCCGGCCAACAGGTGTTTAGATCGCTACAG
GCGATGGAAGCAGGTTTTTA

Downstream 100 bases:

>100_bases
GGACAAGGGTTCGGCAACATAGTGCTGGATCGGGCGATATGCTCTCTCCACCGTCGTGCTCGTGCTTATCCCGAGCATCT
GCGATCTATCGATTGTACGA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MVTILDRTSSDEKRIRHPEKAHRPDTEVMRKPEWIRVKAPTSKGYHETRELVRSHKLVTVCEEAGCPNIGECWEKKHATF
MIMGEICTRACAFCNVATGKPNALDMDEPENVAKAVKQMGLSHVVITSVDRDDLADGGAEHFEKVIWAIRAASPTTTIEI
LTPDFLKKPGALERVVAAKPDVFNHNMETVPGNYLTVRPGARYFHSVRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
MDDLRSADVDFLTIGQYLQPTRKHHKVEAFVTPEEFKSYETVAYAKGFLMVSSSPLTRSSHHAGDDFERLRAAREKKLLA
AAE

Sequences:

>Translated_323_residues
MVTILDRTSSDEKRIRHPEKAHRPDTEVMRKPEWIRVKAPTSKGYHETRELVRSHKLVTVCEEAGCPNIGECWEKKHATF
MIMGEICTRACAFCNVATGKPNALDMDEPENVAKAVKQMGLSHVVITSVDRDDLADGGAEHFEKVIWAIRAASPTTTIEI
LTPDFLKKPGALERVVAAKPDVFNHNMETVPGNYLTVRPGARYFHSVRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
MDDLRSADVDFLTIGQYLQPTRKHHKVEAFVTPEEFKSYETVAYAKGFLMVSSSPLTRSSHHAGDDFERLRAAREKKLLA
AAE
>Mature_323_residues
MVTILDRTSSDEKRIRHPEKAHRPDTEVMRKPEWIRVKAPTSKGYHETRELVRSHKLVTVCEEAGCPNIGECWEKKHATF
MIMGEICTRACAFCNVATGKPNALDMDEPENVAKAVKQMGLSHVVITSVDRDDLADGGAEHFEKVIWAIRAASPTTTIEI
LTPDFLKKPGALERVVAAKPDVFNHNMETVPGNYLTVRPGARYFHSVRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
MDDLRSADVDFLTIGQYLQPTRKHHKVEAFVTPEEFKSYETVAYAKGFLMVSSSPLTRSSHHAGDDFERLRAAREKKLLA
AAE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=320, Percent_Identity=44.6875, Blast_Score=276, Evalue=1e-74,
Organism=Homo sapiens, GI37577164, Length=272, Percent_Identity=44.8529411764706, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=50.7194244604317, Blast_Score=285, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI32564533, Length=256, Percent_Identity=45.3125, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=42.5742574257426, Blast_Score=248, Evalue=8e-67,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=44.7552447552448, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36341; Mature: 36341

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTILDRTSSDEKRIRHPEKAHRPDTEVMRKPEWIRVKAPTSKGYHETRELVRSHKLVTV
CEEEECCCCCCHHHHCCCHHHCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHEE
CEEAGCPNIGECWEKKHATFMIMGEICTRACAFCNVATGKPNALDMDEPENVAKAVKQMG
HHHCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
LSHVVITSVDRDDLADGGAEHFEKVIWAIRAASPTTTIEILTPDFLKKPGALERVVAAKP
CCEEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHCCCHHHHHHHCCC
DVFNHNMETVPGNYLTVRPGARYFHSVRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
CHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHEECCCEEEECCCCHHHHHHH
MDDLRSADVDFLTIGQYLQPTRKHHKVEAFVTPEEFKSYETVAYAKGFLMVSSSPLTRSS
HHHHHHCCCCEEEHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCEEEEECCCCCCCC
HHAGDDFERLRAAREKKLLAAAE
CCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVTILDRTSSDEKRIRHPEKAHRPDTEVMRKPEWIRVKAPTSKGYHETRELVRSHKLVTV
CEEEECCCCCCHHHHCCCHHHCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHEE
CEEAGCPNIGECWEKKHATFMIMGEICTRACAFCNVATGKPNALDMDEPENVAKAVKQMG
HHHCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
LSHVVITSVDRDDLADGGAEHFEKVIWAIRAASPTTTIEILTPDFLKKPGALERVVAAKP
CCEEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHCCCHHHHHHHCCC
DVFNHNMETVPGNYLTVRPGARYFHSVRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
CHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHEECCCEEEECCCCHHHHHHH
MDDLRSADVDFLTIGQYLQPTRKHHKVEAFVTPEEFKSYETVAYAKGFLMVSSSPLTRSS
HHHHHHCCCCEEEHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCEEEEECCCCCCCC
HHAGDDFERLRAAREKKLLAAAE
CCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA