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The map label for this gene is pleD [H]

Identifier: 15888624

GI number: 15888624

Start: 1286797

End: 1288167

Strand: Direct

Name: pleD [H]

Synonym: Atu1297

Alternate gene names: 15888624

Gene position: 1286797-1288167 (Clockwise)

Preceding gene: 159184699

Following gene: 159184700

Centisome position: 45.28

GC content: 60.47

Gene sequence:

>1371_bases
ATGACGGCGAGAGTTCTGGTTGTTGACGACATACCCGCAAACGTGAAGCTTCTCGAGGCTCGGCTTGTGGCGGAATATTT
CGACGTCGTCACCGCAGAGGATGGTTTCAAGGCGCTGGCGATCTGCGATGAAGAGCAGGTCGACATCATTCTGCTCGATA
TCATGATGCCGGGCATGGATGGTTTCGAAGTCTGTGAAAGGCTGAAGGCCAATCCAAACACCGCCCATATTCCGGTTGTC
ATGGTCACCGCGCTCGATCAGCCGTCGGACCGTGTACGCGGCCTGAAGGCCGGCGCCGACGATTTTCTCACGAAACCGGT
CAACGATCTTCAGCTGATCGCCCGTGTCAAAAGCCTGGTTCGTCTGAAGGCCGTGAGTGACGAGTTGCGGCTGCGTGCGG
AAACTGCGCGGCAGATCGGCATCGAGGAGATGCTGCGGAGCGACGGCCTGATGCAGACGCCGGGCCGCGTGCTGGTTGCG
GATGGTCGCGCCAGCTCGCAGGAACGCATCATCCGGGCGCTGAAGCCGGTCGCGGAGGTCGATGCCGTCACGGAGCCGCA
GGCGGCTCTGCTCAAGGCGGCGAGCAGCCCCTTCGAGCTCGTCATCGTCAATTCCAATTTCGAGGACTACGATCCGCTGA
GACTGTGTTCGCAGTTTCGCTCGCTGGAGCGCACCCGTTTTCTGCCGTTGCTTCTGGTGGCGGAGCAGGGGGCGGATGAG
ATGGTGGCGCGTGCGCTCGATCTTGGGGTGAACGATTATATTCTCCGCCCGATCGATCCGAATGAACTCGTCGCCCGTTC
GCTGACGCAGATCAGGCGCAAGCGCTATAATGAACATCTGCGGCTCAATCTGCAGCATACGATGGAGCTTGCGATTGTAG
ACGGGCTGACGGGCCTCAACAATCGTCGTTATCTCGATAGTCACCTGAAGATACTTTTCGACCGGGCTGCCGTGCGTGGT
CGCCCGATCTCCATCTGCATGACCGATATAGACCGCTTCAAGCTGGTCAATGACACCTATGGCCACGATGTCGGCGATGA
GGTGCTACGGGAATTTGCAGCACGCATCCGCAGCACGGTGCGCGGCGCGGATCTCGCCTGCCGTTATGGCGGAGAGGAAT
TCGTCGTCGTCATGCCGGATACGCCCATCGAGCTTGCGGCAAGCGTCGCCGAGCGCCTGCGGGCAATCGTGGAAGACAAG
CCCTTTTATGTTCGTTCCATCGATCGGGAACTGAGCATCACCGCCTCCCTCGGCATCGCCACCAGCAGTGGCGCCTTCGG
CGCGCCGGACGAAATATTGAAGCAGGCCGACAAGGCGCTTTACGAGGCGAAACATGCCGGACGCAACCGCGTCGTGGCCG
CCGCGGCCTGA

Upstream 100 bases:

>100_bases
CGGATGTGAGGCCTATGTGTCCAAACCGATTTCCGTTCCGAAATTCATCGAAATCATCAAGACCTATCTTGGCGATGCGT
AAGGCGGTTGGGGAACTGTT

Downstream 100 bases:

>100_bases
GCCGGCCGCGGGCAATTAACCACGACTGTTTGCGCAGCCTCTCCGGCATATCCCGGCGAAATAGCTATTCGCAAATCGAA
GCCGACGTCGCGAGACCGCA

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTARVLVVDDIPANVKLLEARLVAEYFDVVTAEDGFKALAICDEEQVDIILLDIMMPGMDGFEVCERLKANPNTAHIPVV
MVTALDQPSDRVRGLKAGADDFLTKPVNDLQLIARVKSLVRLKAVSDELRLRAETARQIGIEEMLRSDGLMQTPGRVLVA
DGRASSQERIIRALKPVAEVDAVTEPQAALLKAASSPFELVIVNSNFEDYDPLRLCSQFRSLERTRFLPLLLVAEQGADE
MVARALDLGVNDYILRPIDPNELVARSLTQIRRKRYNEHLRLNLQHTMELAIVDGLTGLNNRRYLDSHLKILFDRAAVRG
RPISICMTDIDRFKLVNDTYGHDVGDEVLREFAARIRSTVRGADLACRYGGEEFVVVMPDTPIELAASVAERLRAIVEDK
PFYVRSIDRELSITASLGIATSSGAFGAPDEILKQADKALYEAKHAGRNRVVAAAA

Sequences:

>Translated_456_residues
MTARVLVVDDIPANVKLLEARLVAEYFDVVTAEDGFKALAICDEEQVDIILLDIMMPGMDGFEVCERLKANPNTAHIPVV
MVTALDQPSDRVRGLKAGADDFLTKPVNDLQLIARVKSLVRLKAVSDELRLRAETARQIGIEEMLRSDGLMQTPGRVLVA
DGRASSQERIIRALKPVAEVDAVTEPQAALLKAASSPFELVIVNSNFEDYDPLRLCSQFRSLERTRFLPLLLVAEQGADE
MVARALDLGVNDYILRPIDPNELVARSLTQIRRKRYNEHLRLNLQHTMELAIVDGLTGLNNRRYLDSHLKILFDRAAVRG
RPISICMTDIDRFKLVNDTYGHDVGDEVLREFAARIRSTVRGADLACRYGGEEFVVVMPDTPIELAASVAERLRAIVEDK
PFYVRSIDRELSITASLGIATSSGAFGAPDEILKQADKALYEAKHAGRNRVVAAAA
>Mature_455_residues
TARVLVVDDIPANVKLLEARLVAEYFDVVTAEDGFKALAICDEEQVDIILLDIMMPGMDGFEVCERLKANPNTAHIPVVM
VTALDQPSDRVRGLKAGADDFLTKPVNDLQLIARVKSLVRLKAVSDELRLRAETARQIGIEEMLRSDGLMQTPGRVLVAD
GRASSQERIIRALKPVAEVDAVTEPQAALLKAASSPFELVIVNSNFEDYDPLRLCSQFRSLERTRFLPLLLVAEQGADEM
VARALDLGVNDYILRPIDPNELVARSLTQIRRKRYNEHLRLNLQHTMELAIVDGLTGLNNRRYLDSHLKILFDRAAVRGR
PISICMTDIDRFKLVNDTYGHDVGDEVLREFAARIRSTVRGADLACRYGGEEFVVVMPDTPIELAASVAERLRAIVEDKP
FYVRSIDRELSITASLGIATSSGAFGAPDEILKQADKALYEAKHAGRNRVVAAAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=167, Percent_Identity=38.9221556886228, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787262, Length=179, Percent_Identity=39.6648044692737, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87081881, Length=190, Percent_Identity=40, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=37.888198757764, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI87082007, Length=190, Percent_Identity=38.4210526315789, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=36.7088607594937, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=37.9518072289157, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=42.1487603305785, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=33.9506172839506, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=33.7278106508876, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=36.3636363636364, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788394, Length=120, Percent_Identity=37.5, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081977, Length=167, Percent_Identity=32.9341317365269, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787541, Length=181, Percent_Identity=32.0441988950276, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=33.8983050847458, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=115, Percent_Identity=33.9130434782609, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790863, Length=158, Percent_Identity=31.0126582278481, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI2367329, Length=153, Percent_Identity=35.9477124183007, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=38.8349514563107, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=33.0508474576271, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789149, Length=108, Percent_Identity=37.037037037037, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=31.0975609756098, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787056, Length=165, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790860, Length=172, Percent_Identity=31.3953488372093, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787229, Length=120, Percent_Identity=30.8333333333333, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1790437, Length=139, Percent_Identity=35.2517985611511, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788905, Length=131, Percent_Identity=35.8778625954198, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081921, Length=165, Percent_Identity=29.0909090909091, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788713, Length=126, Percent_Identity=30.952380952381, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788550, Length=134, Percent_Identity=30.5970149253731, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1787375, Length=118, Percent_Identity=33.0508474576271, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50524; Mature: 50393

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARVLVVDDIPANVKLLEARLVAEYFDVVTAEDGFKALAICDEEQVDIILLDIMMPGMD
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCC
GFEVCERLKANPNTAHIPVVMVTALDQPSDRVRGLKAGADDFLTKPVNDLQLIARVKSLV
HHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKAVSDELRLRAETARQIGIEEMLRSDGLMQTPGRVLVADGRASSQERIIRALKPVAEV
HHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
DAVTEPQAALLKAASSPFELVIVNSNFEDYDPLRLCSQFRSLERTRFLPLLLVAEQGADE
HCCCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHH
MVARALDLGVNDYILRPIDPNELVARSLTQIRRKRYNEHLRLNLQHTMELAIVDGLTGLN
HHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHEEEEHHHHEEEEEECCCCCCC
NRRYLDSHLKILFDRAAVRGRPISICMTDIDRFKLVNDTYGHDVGDEVLREFAARIRSTV
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHEEECCCCCCCCHHHHHHHHHHHHHHHH
RGADLACRYGGEEFVVVMPDTPIELAASVAERLRAIVEDKPFYVRSIDRELSITASLGIA
CCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECEE
TSSGAFGAPDEILKQADKALYEAKHAGRNRVVAAAA
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
TARVLVVDDIPANVKLLEARLVAEYFDVVTAEDGFKALAICDEEQVDIILLDIMMPGMD
CEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCC
GFEVCERLKANPNTAHIPVVMVTALDQPSDRVRGLKAGADDFLTKPVNDLQLIARVKSLV
HHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKAVSDELRLRAETARQIGIEEMLRSDGLMQTPGRVLVADGRASSQERIIRALKPVAEV
HHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
DAVTEPQAALLKAASSPFELVIVNSNFEDYDPLRLCSQFRSLERTRFLPLLLVAEQGADE
HCCCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHH
MVARALDLGVNDYILRPIDPNELVARSLTQIRRKRYNEHLRLNLQHTMELAIVDGLTGLN
HHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHEEEEHHHHEEEEEECCCCCCC
NRRYLDSHLKILFDRAAVRGRPISICMTDIDRFKLVNDTYGHDVGDEVLREFAARIRSTV
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHEEECCCCCCCCHHHHHHHHHHHHHHHH
RGADLACRYGGEEFVVVMPDTPIELAASVAERLRAIVEDKPFYVRSIDRELSITASLGIA
CCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECEE
TSSGAFGAPDEILKQADKALYEAKHAGRNRVVAAAA
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA