Click here to switch to the map view.

The map label for this gene is aspS [H]

Identifier: 15888491

GI number: 15888491

Start: 1144725

End: 1146512

Strand: Direct

Name: aspS [H]

Synonym: Atu1153

Alternate gene names: 15888491

Gene position: 1144725-1146512 (Clockwise)

Preceding gene: 15888482

Following gene: 15888492

Centisome position: 40.28

GC content: 60.46

Gene sequence:

>1788_bases
ATGCATCGTTATCGCAGCCACACCTGTGCCGCTCTCCGCAAGTCCGACGTCGGCGAAACCGTTCGCCTTTCCGGTTGGGT
TCATCGCGTGCGAGATCATGGCGGCGTTCTCTTCATCGACCTTCGCGACCATTACGGCATCACCCAGGTGGTGGCCGATC
CTGATAGCCCGGCCTTCAAGGTTGCAGAAACCGTGCGCGGCGAATGGGTCATCCGCATCGACGGTCTAGTCAAGGCGCGT
TCGGAAGACACCATCAACAAAGGCATGGCGACCGGCGAGATCGAGCTTTACGCGCAGGAGATCGAAGTTCTTGGTGTTGC
CAAGGAACTGCCACTGCCGGTGTTCGGCGAGCCTGAATATCCCGAAGACGTCCGCCTGAAGTATCGCTTCCTCGACCTTC
GCCGCGAAACCCTGCACAGGAACATCGTCAAGCGCACGCAGATCATCTCCTCCATGCGCAAGGGCATGGGCGATCTGGGC
TTTGCCGAATATACGACGCCGATCCTCACTGCATCCTCGCCGGAAGGCGCGCGCGACTTCCTCGTGCCCTCGCGCATCCA
CGAAGGTCAGTTCTTCGCGCTGCCGCAGGCGCCGCAGCAGTACAAGCAGCTTCTGATGGTTGCCGGTTTCGACCGCTACT
TCCAGATCGCCCCCTGCTTCCGCGATGAAGACCCGCGTGCCGACCGTCTGCCGGGCGAATTCTACCAGCTCGACGTCGAA
ATGAGCTTCGTGACCCAGGAAGATGTCTGGACCACGATGGAGCCGATGATGACGGCCGTGTTCGAACAGTTTGCCGAAGG
CAAGCCGGTGACGAAACAGTGGCCGCGCATCCCCTATGACGAATCGATCCGTAAATACGGTTCCGACAAGCCGGACCTGC
GTAACCCGATCGTCATGGAAGCCGTGACTGAGCATTTCGACGGTTCGGGCTTCAAGGTCTTCGCCAACATGATCGCTTCC
AACCCCAAGGTTCAGGTCTGGGCGATCCCCGCCAAGACCGGCGGTTCGCGCGCTTTCTGCGACCGCATGAACGCCTGGGC
GCAGTCTCAGGGCCAACCCGGCCTCGGCTACATCTTCTGGAAGGAAGAAGAAGGCAAGGTTGCCGGTTCGGGTCCGCTTG
CCAAGAACATCGGCGAAGAACGCACCGAGGCGCTGCGCCAGCAGCTCGGTCTCGAGGCGGGCGATGCCTGCTTCTTCGTT
GCTGGTGATCCCGCCAAGTTCTACAAGTTCGCCGGCGAGGCGCGCACCCGCGCTGCCGACGAGTTGAACCTGATCGACCG
CGACCGCTTCGAAATGTGCTGGATCGTCGATTTCCCGTTCTTCGAATACAACGAAGAAGAAAAGAAGATCGATTTCGCCC
ACAACCCCTTCTCCATGCCGCAGGGTGGAATGGAAGCGCTGGAAGGGCAGGACCCGCTCTCCATCAAGGCGTTCCAGTAT
GACGCGGTCTGCAACGGTTTTGAAATCGCTTCCGGCTCGATCCGTAACCAGTCGCCCGAGCTGATGGTCAAGGCTTTCGA
AAAGGTTGGCCTCTCGCAGAGCGATGTGGAAGAGCGCTTCGGCGGTCTCTATCGTGCCTTCCAGTATGGCGCGCCTCCGC
ATGGCGGTTGCGCCTTCGGTATCGACCGTGTCGTCATGCTGCTGGTCGGTGCGAAGAACCTGCGCGAAATCACGCTGTTC
CCGATGAACCAGCAGGCGCAGGACCTTCTGATGAATGCGCCGTCGCCGGCAACGCCAACACAGCTTCGCGAACTGGCGCT
GCGCGTCGTTCCGTCCAAGAAGGACTGA

Upstream 100 bases:

>100_bases
TCTTGCGCTTTGCCTTGACAAATCAGGCTGGTCATGCGCTTTTCCGGCAAATTTCGACAGTGCCGCCTCTCTGGCCCACG
ACACGTTCCAGGATATAAAA

Downstream 100 bases:

>100_bases
TCATGTTGAAGACGCTGCTCGACCGTGTTCGCGCAGCCTTCACCCGTGCATTGGCGGCCGCGCTTGCGGCCGCTTTTGCG
TTCTGGGTCGCGCATCGGTG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MHRYRSHTCAALRKSDVGETVRLSGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFKVAETVRGEWVIRIDGLVKAR
SEDTINKGMATGEIELYAQEIEVLGVAKELPLPVFGEPEYPEDVRLKYRFLDLRRETLHRNIVKRTQIISSMRKGMGDLG
FAEYTTPILTASSPEGARDFLVPSRIHEGQFFALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVTQEDVWTTMEPMMTAVFEQFAEGKPVTKQWPRIPYDESIRKYGSDKPDLRNPIVMEAVTEHFDGSGFKVFANMIAS
NPKVQVWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFWKEEEGKVAGSGPLAKNIGEERTEALRQQLGLEAGDACFFV
AGDPAKFYKFAGEARTRAADELNLIDRDRFEMCWIVDFPFFEYNEEEKKIDFAHNPFSMPQGGMEALEGQDPLSIKAFQY
DAVCNGFEIASGSIRNQSPELMVKAFEKVGLSQSDVEERFGGLYRAFQYGAPPHGGCAFGIDRVVMLLVGAKNLREITLF
PMNQQAQDLLMNAPSPATPTQLRELALRVVPSKKD

Sequences:

>Translated_595_residues
MHRYRSHTCAALRKSDVGETVRLSGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFKVAETVRGEWVIRIDGLVKAR
SEDTINKGMATGEIELYAQEIEVLGVAKELPLPVFGEPEYPEDVRLKYRFLDLRRETLHRNIVKRTQIISSMRKGMGDLG
FAEYTTPILTASSPEGARDFLVPSRIHEGQFFALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVTQEDVWTTMEPMMTAVFEQFAEGKPVTKQWPRIPYDESIRKYGSDKPDLRNPIVMEAVTEHFDGSGFKVFANMIAS
NPKVQVWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFWKEEEGKVAGSGPLAKNIGEERTEALRQQLGLEAGDACFFV
AGDPAKFYKFAGEARTRAADELNLIDRDRFEMCWIVDFPFFEYNEEEKKIDFAHNPFSMPQGGMEALEGQDPLSIKAFQY
DAVCNGFEIASGSIRNQSPELMVKAFEKVGLSQSDVEERFGGLYRAFQYGAPPHGGCAFGIDRVVMLLVGAKNLREITLF
PMNQQAQDLLMNAPSPATPTQLRELALRVVPSKKD
>Mature_595_residues
MHRYRSHTCAALRKSDVGETVRLSGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFKVAETVRGEWVIRIDGLVKAR
SEDTINKGMATGEIELYAQEIEVLGVAKELPLPVFGEPEYPEDVRLKYRFLDLRRETLHRNIVKRTQIISSMRKGMGDLG
FAEYTTPILTASSPEGARDFLVPSRIHEGQFFALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVTQEDVWTTMEPMMTAVFEQFAEGKPVTKQWPRIPYDESIRKYGSDKPDLRNPIVMEAVTEHFDGSGFKVFANMIAS
NPKVQVWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFWKEEEGKVAGSGPLAKNIGEERTEALRQQLGLEAGDACFFV
AGDPAKFYKFAGEARTRAADELNLIDRDRFEMCWIVDFPFFEYNEEEKKIDFAHNPFSMPQGGMEALEGQDPLSIKAFQY
DAVCNGFEIASGSIRNQSPELMVKAFEKVGLSQSDVEERFGGLYRAFQYGAPPHGGCAFGIDRVVMLLVGAKNLREITLF
PMNQQAQDLLMNAPSPATPTQLRELALRVVPSKKD

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=598, Percent_Identity=34.1137123745819, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI45439306, Length=293, Percent_Identity=28.6689419795222, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI226491502, Length=256, Percent_Identity=27.34375, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI4758762, Length=248, Percent_Identity=26.2096774193548, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=41.6243654822335, Blast_Score=440, Evalue=1e-124,
Organism=Escherichia coli, GI1789256, Length=299, Percent_Identity=27.7591973244147, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790571, Length=298, Percent_Identity=26.8456375838926, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=609, Percent_Identity=32.512315270936, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17551876, Length=299, Percent_Identity=26.0869565217391, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=295, Percent_Identity=26.1016949152542, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=248, Percent_Identity=27.0161290322581, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71994340, Length=248, Percent_Identity=27.0161290322581, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17535927, Length=248, Percent_Identity=27.0161290322581, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=658, Percent_Identity=28.2674772036474, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6323011, Length=302, Percent_Identity=27.8145695364238, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=282, Percent_Identity=24.822695035461, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24584738, Length=599, Percent_Identity=33.5559265442404, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI17136276, Length=261, Percent_Identity=28.3524904214559, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921528, Length=226, Percent_Identity=27.4336283185841, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67036; Mature: 67036

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRSHTCAALRKSDVGETVRLSGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFK
CCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHH
VAETVRGEWVIRIDGLVKARSEDTINKGMATGEIELYAQEIEVLGVAKELPLPVFGEPEY
HHHHHCCCEEEEECCEEECCCCHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCC
PEDVRLKYRFLDLRRETLHRNIVKRTQIISSMRKGMGDLGFAEYTTPILTASSPEGARDF
CHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEECCCCCCCHHC
LVPSRIHEGQFFALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVWTTMEPMMTAVFEQFAEGKPVTKQWPRIPYDESIRKYGSDKPDLRNPIVME
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHCCCCCCCCCCCHHHH
AVTEHFDGSGFKVFANMIASNPKVQVWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFW
HHHHHCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
KEEEGKVAGSGPLAKNIGEERTEALRQQLGLEAGDACFFVAGDPAKFYKFAGEARTRAAD
ECCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCHHHHHHHH
ELNLIDRDRFEMCWIVDFPFFEYNEEEKKIDFAHNPFSMPQGGMEALEGQDPLSIKAFQY
HCCCCCCCCCCEEEEEECCCCCCCCHHCCEEECCCCCCCCCCCHHHHCCCCCCEEEEEEE
DAVCNGFEIASGSIRNQSPELMVKAFEKVGLSQSDVEERFGGLYRAFQYGAPPHGGCAFG
CHHCCCEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IDRVVMLLVGAKNLREITLFPMNQQAQDLLMNAPSPATPTQLRELALRVVPSKKD
HHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHRYRSHTCAALRKSDVGETVRLSGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFK
CCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHH
VAETVRGEWVIRIDGLVKARSEDTINKGMATGEIELYAQEIEVLGVAKELPLPVFGEPEY
HHHHHCCCEEEEECCEEECCCCHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCC
PEDVRLKYRFLDLRRETLHRNIVKRTQIISSMRKGMGDLGFAEYTTPILTASSPEGARDF
CHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEECCCCCCCHHC
LVPSRIHEGQFFALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVWTTMEPMMTAVFEQFAEGKPVTKQWPRIPYDESIRKYGSDKPDLRNPIVME
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHCCCCCCCCCCCHHHH
AVTEHFDGSGFKVFANMIASNPKVQVWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFW
HHHHHCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
KEEEGKVAGSGPLAKNIGEERTEALRQQLGLEAGDACFFVAGDPAKFYKFAGEARTRAAD
ECCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCHHHHHHHH
ELNLIDRDRFEMCWIVDFPFFEYNEEEKKIDFAHNPFSMPQGGMEALEGQDPLSIKAFQY
HCCCCCCCCCCEEEEEECCCCCCCCHHCCEEECCCCCCCCCCCHHHHCCCCCCEEEEEEE
DAVCNGFEIASGSIRNQSPELMVKAFEKVGLSQSDVEERFGGLYRAFQYGAPPHGGCAFG
CHHCCCEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IDRVVMLLVGAKNLREITLFPMNQQAQDLLMNAPSPATPTQLRELALRVVPSKKD
HHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA