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The map label for this gene is dapA

Identifier: 15888366

GI number: 15888366

Start: 1017629

End: 1018513

Strand: Direct

Name: dapA

Synonym: Atu1024

Alternate gene names: 15888366

Gene position: 1017629-1018513 (Clockwise)

Preceding gene: 159184581

Following gene: 159184582

Centisome position: 35.81

GC content: 61.02

Gene sequence:

>885_bases
ATGTTCAAGGGATCAATTCCCGCCCTCATTACCCCGTTCACGGACAATGGTTCCGTGGACGAAAAGGCCTTTGCCGCCCA
TGTGGAATGGCAGATCGCCGAAGGCAGCAACGGCCTTGTGCCCGTGGGCACGACGGGGGAATCCCCGACGCTCTCTCACG
ATGAGCACAAACGGGTCGTTGAGCTGTGCATCGAGGTTGCGGCGAAACGGGTTCCGGTCATTGCGGGTGCTGGCTCCAAC
AATACCGATGAAGCAATCGAGCTTGCGCTGCACGCGCAGGAGGCCGGTGCGGATGCCCTGCTGGTCGTCACTCCCTATTA
CAACAAGCCGACGCAGAAGGGCCTGTTCGCGCATTTTTCCGCTGTCGCCGAAGCGGTGAAACTGCCGATCGTCATCTACA
ATATCCCGCCGCGCTCGGTGGTGGACATGTCGCCGGAAACCATGGGTGCGCTGGTCAAAGCGCATAAGAATATCATCGGC
GTCAAGGATGCGACCGGCAAACTCGATCGGGTTTCCGAACAGCGCATTTCCTGCGGCAAGGATTTTGTCCAGCTGTCGGG
CGAGGATGGCACGGCGCTCGGCTTTAACGCCCATGGCGGCGTCGGCTGCATTTCGGTCACCGCCAATGTCGCGCCGCGCC
TTTGTTCCGAGTTTCAGGCGGCAATGCTTGCCGGTGATTACGCCAAGGCGCTTGAGTATCAGGACCGCCTGATGCCGCTG
CACAGGGCGATCTTCATGGAGCCCGGCGTCTGCGGCACCAAATATGCGCTGTCGAAGACACGCGGCGGCAATCGCCGGGT
CCGTTCGCCCTTGATGAGCACGCTGGAACCGGCAACGGAAGCGGCGATCGATGCGGCGCTGAAGCATGCCGGCCTGATGA
ACTGA

Upstream 100 bases:

>100_bases
GCCTTTGCGCTTGTCGGCGCCAAGGGCTGGCTCTAATGTGTGCCGGTTTTAACCATCGAATGCGGCCGAAGCATGAACCG
CTCGGCGCCAGGAGCTTTGC

Downstream 100 bases:

>100_bases
GATACGCCTGAAAAGCTTGAAAGGCGCGCCATGGACCGCTTCATAACAGAAGCCCTCCCGCGCGCCTTTTCTTTTTGCGG
CGACAACATTAAATAGATGT

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MFKGSIPALITPFTDNGSVDEKAFAAHVEWQIAEGSNGLVPVGTTGESPTLSHDEHKRVVELCIEVAAKRVPVIAGAGSN
NTDEAIELALHAQEAGADALLVVTPYYNKPTQKGLFAHFSAVAEAVKLPIVIYNIPPRSVVDMSPETMGALVKAHKNIIG
VKDATGKLDRVSEQRISCGKDFVQLSGEDGTALGFNAHGGVGCISVTANVAPRLCSEFQAAMLAGDYAKALEYQDRLMPL
HRAIFMEPGVCGTKYALSKTRGGNRRVRSPLMSTLEPATEAAIDAALKHAGLMN

Sequences:

>Translated_294_residues
MFKGSIPALITPFTDNGSVDEKAFAAHVEWQIAEGSNGLVPVGTTGESPTLSHDEHKRVVELCIEVAAKRVPVIAGAGSN
NTDEAIELALHAQEAGADALLVVTPYYNKPTQKGLFAHFSAVAEAVKLPIVIYNIPPRSVVDMSPETMGALVKAHKNIIG
VKDATGKLDRVSEQRISCGKDFVQLSGEDGTALGFNAHGGVGCISVTANVAPRLCSEFQAAMLAGDYAKALEYQDRLMPL
HRAIFMEPGVCGTKYALSKTRGGNRRVRSPLMSTLEPATEAAIDAALKHAGLMN
>Mature_294_residues
MFKGSIPALITPFTDNGSVDEKAFAAHVEWQIAEGSNGLVPVGTTGESPTLSHDEHKRVVELCIEVAAKRVPVIAGAGSN
NTDEAIELALHAQEAGADALLVVTPYYNKPTQKGLFAHFSAVAEAVKLPIVIYNIPPRSVVDMSPETMGALVKAHKNIIG
VKDATGKLDRVSEQRISCGKDFVQLSGEDGTALGFNAHGGVGCISVTANVAPRLCSEFQAAMLAGDYAKALEYQDRLMPL
HRAIFMEPGVCGTKYALSKTRGGNRRVRSPLMSTLEPATEAAIDAALKHAGLMN

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=296, Percent_Identity=28.0405405405405, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI13540533, Length=241, Percent_Identity=29.4605809128631, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788823, Length=295, Percent_Identity=45.0847457627119, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1786463, Length=244, Percent_Identity=36.4754098360656, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI87082415, Length=260, Percent_Identity=33.4615384615385, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1789620, Length=237, Percent_Identity=29.535864978903, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_AGRT5 (Q8UGL3)

Other databases:

- EMBL:   AE007869
- PIR:   AG2702
- PIR:   G97484
- RefSeq:   NP_354047.1
- ProteinModelPortal:   Q8UGL3
- SMR:   Q8UGL3
- STRING:   Q8UGL3
- GeneID:   1133062
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1024
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   ELLCYPM
- PhylomeDB:   Q8UGL3
- ProtClustDB:   PRK03170
- BioCyc:   ATUM176299-1:ATU1024-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31133; Mature: 31133

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 162-162 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGSIPALITPFTDNGSVDEKAFAAHVEWQIAEGSNGLVPVGTTGESPTLSHDEHKRVV
CCCCCCCEEEEECCCCCCCCHHHEEEEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHHH
ELCIEVAAKRVPVIAGAGSNNTDEAIELALHAQEAGADALLVVTPYYNKPTQKGLFAHFS
HHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHH
AVAEAVKLPIVIYNIPPRSVVDMSPETMGALVKAHKNIIGVKDATGKLDRVSEQRISCGK
HHHHHHHCCEEEEECCCCCEECCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCC
DFVQLSGEDGTALGFNAHGGVGCISVTANVAPRLCSEFQAAMLAGDYAKALEYQDRLMPL
HHEEECCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRAIFMEPGVCGTKYALSKTRGGNRRVRSPLMSTLEPATEAAIDAALKHAGLMN
HHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFKGSIPALITPFTDNGSVDEKAFAAHVEWQIAEGSNGLVPVGTTGESPTLSHDEHKRVV
CCCCCCCEEEEECCCCCCCCHHHEEEEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHHH
ELCIEVAAKRVPVIAGAGSNNTDEAIELALHAQEAGADALLVVTPYYNKPTQKGLFAHFS
HHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHH
AVAEAVKLPIVIYNIPPRSVVDMSPETMGALVKAHKNIIGVKDATGKLDRVSEQRISCGK
HHHHHHHCCEEEEECCCCCEECCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCC
DFVQLSGEDGTALGFNAHGGVGCISVTANVAPRLCSEFQAAMLAGDYAKALEYQDRLMPL
HHEEECCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRAIFMEPGVCGTKYALSKTRGGNRRVRSPLMSTLEPATEAAIDAALKHAGLMN
HHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194