Click here to switch to the map view.

The map label for this gene is pbpF [H]

Identifier: 15888270

GI number: 15888270

Start: 920246

End: 922519

Strand: Reverse

Name: pbpF [H]

Synonym: Atu0931

Alternate gene names: 15888270

Gene position: 922519-920246 (Counterclockwise)

Preceding gene: 15888273

Following gene: 15888269

Centisome position: 32.47

GC content: 62.49

Gene sequence:

>2274_bases
ATGGTTTTGCGTCGGGAATGGGTTGAAACGCAGAAATACCGACGGGCCGCGTTGCCTCGAACAGGAAAACATCGCCAGGT
GCAGGAAGAAAAAGACGATAATAAAGGCCGCAGGAAGCGGCATATCCTGCTCCGGATCGATAGCTTCATCGACTCCGGCC
TGTGGACGGCTGCGGCACGGCTGGTGGATTTCTGGGAAGACGTCACCATCGCCTCGCGCAAACTGCATGTGCGCGGCTGG
AAGAAGCTTGTCGTCAACCTGACCTGTGACGCGCTGACCTTCGGAACGGCCGGCTCGATTGTGCTTCTGGCGCTCGCCAT
GCCTGCTTTCGAGGAAACCAAGAAGGACTGGCGTTATCGCGGCGATTTCGCCGTCACCTTCCTCGATCGTTACGGCAACA
CCATCGGCCATCGCGGCATCATCCATGAAGACAGCGTGCCGATCGACCAGATGCCTGATCATTTCATCAAGGCGGTGCTG
GCCACCGAGGACCGGCGTTTCTTCGACCATTTCGGCATCGATTTCATCGGCCTTGCCCGCGCCATGAGCGAAAATGCCCG
CGCTGGCGGCGTGGTGCAGGGCGGCTCGACGCTGACGCAGCAGCTTGCCAAGAACCTGTTTCTCACCAATGAGCGCTCGC
TGGAGCGCAAGATCAAGGAAGCCTTCCTCGCGCTCTGGCTTGAGAGCAACATGTCGAAGAAGGAAATCCTCAGCCTTTAT
CTCGACCGCGCCTATATGGGCGGCGGCACCTTTGGCGCGGCGGCGGCGGCGCAGTTTTATTTCGGCAAGAACCTGACCGA
CGTGACGCTCTCCGAATCCGCCATTCTGGCCGGCCTGTTCAAGGCGCCGGCCAAATATGCGCCGCATGTCAATTTGCCGG
CGGCCCGCGCCCGCGCCAATACCGTGCTTTCCAACCTGGTTCAGAGCGGGCTGATGACCGAGGGCCAGGTGATTGGCGCA
CGGCGCAACCCGGCCACCGTCATCGACCGCGCCGATGTGAAGGCGCCGGATTATTTCCTCGACTGGGCCTTCGACGAGGT
GCAACGACTGGCCGCCGAAGGCAAGTTTAAGGATCATACCGTCGTTGTGCGCACCACCATCGATACCGGCCTGCAGCAGG
CCGCCGAGCAGGCGATGGAAATGGAGCTGCGTGAATATGGCGAGGGTTACCGCGTCAAACAGGGCGCGATGGTGATGATC
GAGAATGGCGGCGGCGTGCGCGCCATGGTCGGCGGGCGTGATTATGGCGAAAGCCAGTTCAACCGCGCCACGGCGGCGCT
GCGCCAGCCGGGCTCTTCCTTCAAGGTCTACACCTACTCGGCCGCGATGGAAAAGGGCATGAAGCCGGACACGCTGATAT
CAGACGCGCCGGTGACCTGGCGCGGCTGGTCGCCTCAGAATTACGGCCGCTCCTATGCCGGCAAGGTGACGCTGCAGGTA
GCACTCGCCAAATCCTACAATACCGTGCCGGTCCGGCTTGCCAAGGACGTGCTAGGCACGCAGGTGATCGTCGACACCGC
CAAGGCAATGGGTGTCGCCACACCGCTTCGCAGCGACAAGACCATTCCGCTCGGCACGTCGGAAGTCACGGTTCTCGATC
AGGCCACCGCCTATGCCGTGTTCCCGGCCGACGGTATGCAGTCCCGCCGCCACGGCATCGAGCAGGTGATGGATTACGAG
GGCAACGTTCTCTACGATTTCAGCCATGACGAGCCGCCCGCCAAGCGGGTGCTTTCCGAAGAAGCCAATTCGAAGATGAA
CCAGATGCTGGTGACCATTCCGGTGATGGGCACGGCCCGGCGCGGCGCACTGGATAACGGCATCGTCTCCGGCGGCAAGA
CCGGCACCTCACAGGCTTATCGCGACGCCTGGTATGTGGGCTTTACCGGCAACTATACGACCGCTGTCTGGTTCGGCAAT
GACGAGTTCACGCCGATGAACAACATGACCGGCGGCGCGCTTCCCGCCATGACCTACAAGCGGCTGATGGACTATGCCCA
TCAGGGCATGGAGCTGCGCCCCATTCCCGGCATCCAGAACCTGCTGCCGACAGGCGCGCGTCCGCAGCCTTCGGCACAGG
TCGCCGCCGCATCCGCCACCACTGCGCCCGCCATGCCGGCACTCACCCGCCCCCGCTCGCTCTCAGCCGAGGCAACCCGT
GTCATCCGGTCCATCGCCAAGACGATGAAGGAAGCCCCGCCTCTAGCGGTCACGACGCAGAAGGTGGCCGAAGCCACTAT
CCGCGATGGCAGGGATGAAGCGGGGCGGCGTTAG

Upstream 100 bases:

>100_bases
AGGGCTGGTTTGTACGATATTTTATGACGATTGGCGGGCGGCAGAGGGTCATTGGCCCGCAAGAAGCCGCCGCGTCAAGC
AATTCCGACACAAGCATGCC

Downstream 100 bases:

>100_bases
GATACGTCCCATGCCGCCACTGGAAATGGAGTTTCGCGACCCTTGCGGCTGCAGGAACATTCATTCTCTCAAACAACCAG
AGGGTGTTGATCGAGCAAAT

Product: penicillin binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 757; Mature: 757

Protein sequence:

>757_residues
MVLRREWVETQKYRRAALPRTGKHRQVQEEKDDNKGRRKRHILLRIDSFIDSGLWTAAARLVDFWEDVTIASRKLHVRGW
KKLVVNLTCDALTFGTAGSIVLLALAMPAFEETKKDWRYRGDFAVTFLDRYGNTIGHRGIIHEDSVPIDQMPDHFIKAVL
ATEDRRFFDHFGIDFIGLARAMSENARAGGVVQGGSTLTQQLAKNLFLTNERSLERKIKEAFLALWLESNMSKKEILSLY
LDRAYMGGGTFGAAAAAQFYFGKNLTDVTLSESAILAGLFKAPAKYAPHVNLPAARARANTVLSNLVQSGLMTEGQVIGA
RRNPATVIDRADVKAPDYFLDWAFDEVQRLAAEGKFKDHTVVVRTTIDTGLQQAAEQAMEMELREYGEGYRVKQGAMVMI
ENGGGVRAMVGGRDYGESQFNRATAALRQPGSSFKVYTYSAAMEKGMKPDTLISDAPVTWRGWSPQNYGRSYAGKVTLQV
ALAKSYNTVPVRLAKDVLGTQVIVDTAKAMGVATPLRSDKTIPLGTSEVTVLDQATAYAVFPADGMQSRRHGIEQVMDYE
GNVLYDFSHDEPPAKRVLSEEANSKMNQMLVTIPVMGTARRGALDNGIVSGGKTGTSQAYRDAWYVGFTGNYTTAVWFGN
DEFTPMNNMTGGALPAMTYKRLMDYAHQGMELRPIPGIQNLLPTGARPQPSAQVAAASATTAPAMPALTRPRSLSAEATR
VIRSIAKTMKEAPPLAVTTQKVAEATIRDGRDEAGRR

Sequences:

>Translated_757_residues
MVLRREWVETQKYRRAALPRTGKHRQVQEEKDDNKGRRKRHILLRIDSFIDSGLWTAAARLVDFWEDVTIASRKLHVRGW
KKLVVNLTCDALTFGTAGSIVLLALAMPAFEETKKDWRYRGDFAVTFLDRYGNTIGHRGIIHEDSVPIDQMPDHFIKAVL
ATEDRRFFDHFGIDFIGLARAMSENARAGGVVQGGSTLTQQLAKNLFLTNERSLERKIKEAFLALWLESNMSKKEILSLY
LDRAYMGGGTFGAAAAAQFYFGKNLTDVTLSESAILAGLFKAPAKYAPHVNLPAARARANTVLSNLVQSGLMTEGQVIGA
RRNPATVIDRADVKAPDYFLDWAFDEVQRLAAEGKFKDHTVVVRTTIDTGLQQAAEQAMEMELREYGEGYRVKQGAMVMI
ENGGGVRAMVGGRDYGESQFNRATAALRQPGSSFKVYTYSAAMEKGMKPDTLISDAPVTWRGWSPQNYGRSYAGKVTLQV
ALAKSYNTVPVRLAKDVLGTQVIVDTAKAMGVATPLRSDKTIPLGTSEVTVLDQATAYAVFPADGMQSRRHGIEQVMDYE
GNVLYDFSHDEPPAKRVLSEEANSKMNQMLVTIPVMGTARRGALDNGIVSGGKTGTSQAYRDAWYVGFTGNYTTAVWFGN
DEFTPMNNMTGGALPAMTYKRLMDYAHQGMELRPIPGIQNLLPTGARPQPSAQVAAASATTAPAMPALTRPRSLSAEATR
VIRSIAKTMKEAPPLAVTTQKVAEATIRDGRDEAGRR
>Mature_757_residues
MVLRREWVETQKYRRAALPRTGKHRQVQEEKDDNKGRRKRHILLRIDSFIDSGLWTAAARLVDFWEDVTIASRKLHVRGW
KKLVVNLTCDALTFGTAGSIVLLALAMPAFEETKKDWRYRGDFAVTFLDRYGNTIGHRGIIHEDSVPIDQMPDHFIKAVL
ATEDRRFFDHFGIDFIGLARAMSENARAGGVVQGGSTLTQQLAKNLFLTNERSLERKIKEAFLALWLESNMSKKEILSLY
LDRAYMGGGTFGAAAAAQFYFGKNLTDVTLSESAILAGLFKAPAKYAPHVNLPAARARANTVLSNLVQSGLMTEGQVIGA
RRNPATVIDRADVKAPDYFLDWAFDEVQRLAAEGKFKDHTVVVRTTIDTGLQQAAEQAMEMELREYGEGYRVKQGAMVMI
ENGGGVRAMVGGRDYGESQFNRATAALRQPGSSFKVYTYSAAMEKGMKPDTLISDAPVTWRGWSPQNYGRSYAGKVTLQV
ALAKSYNTVPVRLAKDVLGTQVIVDTAKAMGVATPLRSDKTIPLGTSEVTVLDQATAYAVFPADGMQSRRHGIEQVMDYE
GNVLYDFSHDEPPAKRVLSEEANSKMNQMLVTIPVMGTARRGALDNGIVSGGKTGTSQAYRDAWYVGFTGNYTTAVWFGN
DEFTPMNNMTGGALPAMTYKRLMDYAHQGMELRPIPGIQNLLPTGARPQPSAQVAAASATTAPAMPALTRPRSLSAEATR
VIRSIAKTMKEAPPLAVTTQKVAEATIRDGRDEAGRR

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=538, Percent_Identity=33.0855018587361, Blast_Score=212, Evalue=8e-56,
Organism=Escherichia coli, GI87082258, Length=320, Percent_Identity=38.125, Blast_Score=183, Evalue=4e-47,
Organism=Escherichia coli, GI1788867, Length=547, Percent_Identity=28.3363802559415, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1789601, Length=191, Percent_Identity=37.696335078534, Blast_Score=120, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 83319; Mature: 83319

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLRREWVETQKYRRAALPRTGKHRQVQEEKDDNKGRRKRHILLRIDSFIDSGLWTAAAR
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHEEEEEEHHHHHCHHHHHHHH
LVDFWEDVTIASRKLHVRGWKKLVVNLTCDALTFGTAGSIVLLALAMPAFEETKKDWRYR
HHHHHHHHHHHHHEEECCCCEEEEEEEEECCEECCCCHHHHHHHHHCCHHHHHHHHHEEC
GDFAVTFLDRYGNTIGHRGIIHEDSVPIDQMPDHFIKAVLATEDRRFFDHFGIDFIGLAR
CCEEEEEHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCHHHHHHHCCHHHHHHH
AMSENARAGGVVQGGSTLTQQLAKNLFLTNERSLERKIKEAFLALWLESNMSKKEILSLY
HHHCCCCCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LDRAYMGGGTFGAAAAAQFYFGKNLTDVTLSESAILAGLFKAPAKYAPHVNLPAARARAN
HHHHHCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCHHCCCCCCCCHHHHHHH
TVLSNLVQSGLMTEGQVIGARRNPATVIDRADVKAPDYFLDWAFDEVQRLAAEGKFKDHT
HHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
VVVRTTIDTGLQQAAEQAMEMELREYGEGYRVKQGAMVMIENGGGVRAMVGGRDYGESQF
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCEEEEECCCCCCHHHH
NRATAALRQPGSSFKVYTYSAAMEKGMKPDTLISDAPVTWRGWSPQNYGRSYAGKVTLQV
HHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCEECCCCCEECCCCCHHCCCCCCCEEEEEE
ALAKSYNTVPVRLAKDVLGTQVIVDTAKAMGVATPLRSDKTIPLGTSEVTVLDQATAYAV
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEE
FPADGMQSRRHGIEQVMDYEGNVLYDFSHDEPPAKRVLSEEANSKMNQMLVTIPVMGTAR
ECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCC
RGALDNGIVSGGKTGTSQAYRDAWYVGFTGNYTTAVWFGNDEFTPMNNMTGGALPAMTYK
CCCCCCCCCCCCCCCCCHHHHCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHH
RLMDYAHQGMELRPIPGIQNLLPTGARPQPSAQVAAASATTAPAMPALTRPRSLSAEATR
HHHHHHHCCCEECCCCCHHHHCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCHHHHH
VIRSIAKTMKEAPPLAVTTQKVAEATIRDGRDEAGRR
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MVLRREWVETQKYRRAALPRTGKHRQVQEEKDDNKGRRKRHILLRIDSFIDSGLWTAAAR
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHEEEEEEHHHHHCHHHHHHHH
LVDFWEDVTIASRKLHVRGWKKLVVNLTCDALTFGTAGSIVLLALAMPAFEETKKDWRYR
HHHHHHHHHHHHHEEECCCCEEEEEEEEECCEECCCCHHHHHHHHHCCHHHHHHHHHEEC
GDFAVTFLDRYGNTIGHRGIIHEDSVPIDQMPDHFIKAVLATEDRRFFDHFGIDFIGLAR
CCEEEEEHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCHHHHHHHCCHHHHHHH
AMSENARAGGVVQGGSTLTQQLAKNLFLTNERSLERKIKEAFLALWLESNMSKKEILSLY
HHHCCCCCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LDRAYMGGGTFGAAAAAQFYFGKNLTDVTLSESAILAGLFKAPAKYAPHVNLPAARARAN
HHHHHCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCHHCCCCCCCCHHHHHHH
TVLSNLVQSGLMTEGQVIGARRNPATVIDRADVKAPDYFLDWAFDEVQRLAAEGKFKDHT
HHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
VVVRTTIDTGLQQAAEQAMEMELREYGEGYRVKQGAMVMIENGGGVRAMVGGRDYGESQF
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCEEEEECCCCCCHHHH
NRATAALRQPGSSFKVYTYSAAMEKGMKPDTLISDAPVTWRGWSPQNYGRSYAGKVTLQV
HHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCEECCCCCEECCCCCHHCCCCCCCEEEEEE
ALAKSYNTVPVRLAKDVLGTQVIVDTAKAMGVATPLRSDKTIPLGTSEVTVLDQATAYAV
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEE
FPADGMQSRRHGIEQVMDYEGNVLYDFSHDEPPAKRVLSEEANSKMNQMLVTIPVMGTAR
ECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCC
RGALDNGIVSGGKTGTSQAYRDAWYVGFTGNYTTAVWFGNDEFTPMNNMTGGALPAMTYK
CCCCCCCCCCCCCCCCCHHHHCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHH
RLMDYAHQGMELRPIPGIQNLLPTGARPQPSAQVAAASATTAPAMPALTRPRSLSAEATR
HHHHHHHCCCEECCCCCHHHHCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCHHHHH
VIRSIAKTMKEAPPLAVTTQKVAEATIRDGRDEAGRR
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]