Click here to switch to the map view.

The map label for this gene is mnhD [H]

Identifier: 15888250

GI number: 15888250

Start: 902102

End: 903679

Strand: Direct

Name: mnhD [H]

Synonym: Atu0912

Alternate gene names: 15888250

Gene position: 902102-903679 (Clockwise)

Preceding gene: 15888249

Following gene: 15888251

Centisome position: 31.75

GC content: 62.99

Gene sequence:

>1578_bases
ATGGCTTCCTCCACCACATCAACCGTGACCGATCTTTCCGCAGCGCTTGTGATGGCGCCCGTGCCGCTTGCCGACTGGCT
GATCATCCTGCCCATCGCGCTGTGCATCGGCGCAGGGGCCGTGCTGATGATGATGCGCCACGCCATCCGCCACCATGCCG
CAGTCGCCATTGCCGCACTTTCTTTGCTGGTGATCCTTAATGCAGCACTGCTCTGGAAGGTTGCCACGCAAGGCCCGTTC
ACCATGGTCATGGGCCGCTGGCTGCCGCCCTTCGGCATTGCCTTCACCGCCGATCTCACCGGCGCGCTCCTGTCGCTCGC
CGCCGCCATCGTCGCGCTTGCCGGCGCCATCCATGCTGGCGCGGATATCGATGCCAGCGGCAGGCGTTATGGCTTCTATC
CTTTCCTGATGTTGCTGATGGCGGGCGTCACCGGCGCTTTCCTGACCGGTGATCTCTTCAACCTCTATGTCTGGTTCGAG
GTGCTGCTGATCTCGTCCTTCGGCCTCATCGTGCTCGGTTCCACGCGCGAGCAGATCGATGGCGCACTGAAATACGCCAT
CCTGAACCTCATCGGCACGACCCTGTTCCTGATCACGGTCGGATATCTTTATGCCATCTTCGGCACGCTCAACATGGCCG
ATATTGCGCTCAAGGCAACGGAGCTGCGTGGCACCGCGCCGTTGATGACGCTCGCCAGCCTCTTCGCGCTCGCCTTCGCC
ATGAAGGCCGCCGCCTTTCCGGTGAATTTCTGGCTGCCCGCCTCTTATCACACGCCGCGCATCGTCGTTTCCGCGCTCTT
TGGTGGCCTGCTCACCAAGGTCGGCATCTATTCGCTGCTGCGCGTCATGGTCATGCTGTTTCCGGTCGAGCGGGAAGAAC
TGAGCATCGTCATCGCCATTTCCGCCGCACTCACCATGGTGCTGGGCGCCATGGGCGCACTCGCCCAGAACGATATCCGC
CGCATGCTCGGTTACATCGTCATCTCGGGCATCGGCTACATGATGGCCGGCATCGCCATCGGCACGCCGTCAGGCGTATC
GGGCGCCATCTTTTATGCCCTGCATTCCATGGTCCTGATGACGGCGCTTTATCTCGCCGCCGGCCATGCCGCCCGCCTCG
GCGGCGGTTTCAGCCTCACGTCGCTTGGCGACCTTTACCGGCAGGCCCCGTGGTTTTCGGCACTGGCGCTTGCGCTGTTT
TTCGCCGGTTCCGGCCTGCCACCTTTTTCCGGCTTCTGGCCGAAGGCGGTTCTGGTCAAGTCGGCCATCGATATCGGCGC
CTGGTGGCTCGCCGCCGCCATTCTCGTCTCCGGTTTCATCGCCACCATCGCCTTCGGACGGGTATTCCTGCTCTGTTTCT
GGCGCCCAGTAACGACGTCCGCCGGCCAGCCTGCCTTGCAGCCCGCCGCACGTCCGCCGGCCCCGTCTGTCGCGCCACTG
GTGGGGCTGACTTTGCTGGTGGTGTTCTTCGGCCTGTTCCCGGAAAGCCTGCTCAATCTCAGCCAGCAGGCGGCGGCTGG
TCTTGGTAATCCGCAGGCCTATATTCAGTCGGTCTTTCCGCAAGGGGGAAAGCCATGA

Upstream 100 bases:

>100_bases
CAGGAATTGCAGACCGACGATACGGACGAAATGCGCACCGCAGAACCCGCCGGCGAGCCCTTGCCGCCGCTCGGTTATTG
AGCGGCGAGGCAGAGACGAC

Downstream 100 bases:

>100_bases
GCCTCTACACCGTGCAATTGGTGTTTATTGCCGTCTGGCTCGCCGTGACGGGCAGCCTCACGCTGGCGAATATTGTCTTC
GCCCTCATCGTTTCGACACT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MASSTTSTVTDLSAALVMAPVPLADWLIILPIALCIGAGAVLMMMRHAIRHHAAVAIAALSLLVILNAALLWKVATQGPF
TMVMGRWLPPFGIAFTADLTGALLSLAAAIVALAGAIHAGADIDASGRRYGFYPFLMLLMAGVTGAFLTGDLFNLYVWFE
VLLISSFGLIVLGSTREQIDGALKYAILNLIGTTLFLITVGYLYAIFGTLNMADIALKATELRGTAPLMTLASLFALAFA
MKAAAFPVNFWLPASYHTPRIVVSALFGGLLTKVGIYSLLRVMVMLFPVEREELSIVIAISAALTMVLGAMGALAQNDIR
RMLGYIVISGIGYMMAGIAIGTPSGVSGAIFYALHSMVLMTALYLAAGHAARLGGGFSLTSLGDLYRQAPWFSALALALF
FAGSGLPPFSGFWPKAVLVKSAIDIGAWWLAAAILVSGFIATIAFGRVFLLCFWRPVTTSAGQPALQPAARPPAPSVAPL
VGLTLLVVFFGLFPESLLNLSQQAAAGLGNPQAYIQSVFPQGGKP

Sequences:

>Translated_525_residues
MASSTTSTVTDLSAALVMAPVPLADWLIILPIALCIGAGAVLMMMRHAIRHHAAVAIAALSLLVILNAALLWKVATQGPF
TMVMGRWLPPFGIAFTADLTGALLSLAAAIVALAGAIHAGADIDASGRRYGFYPFLMLLMAGVTGAFLTGDLFNLYVWFE
VLLISSFGLIVLGSTREQIDGALKYAILNLIGTTLFLITVGYLYAIFGTLNMADIALKATELRGTAPLMTLASLFALAFA
MKAAAFPVNFWLPASYHTPRIVVSALFGGLLTKVGIYSLLRVMVMLFPVEREELSIVIAISAALTMVLGAMGALAQNDIR
RMLGYIVISGIGYMMAGIAIGTPSGVSGAIFYALHSMVLMTALYLAAGHAARLGGGFSLTSLGDLYRQAPWFSALALALF
FAGSGLPPFSGFWPKAVLVKSAIDIGAWWLAAAILVSGFIATIAFGRVFLLCFWRPVTTSAGQPALQPAARPPAPSVAPL
VGLTLLVVFFGLFPESLLNLSQQAAAGLGNPQAYIQSVFPQGGKP
>Mature_524_residues
ASSTTSTVTDLSAALVMAPVPLADWLIILPIALCIGAGAVLMMMRHAIRHHAAVAIAALSLLVILNAALLWKVATQGPFT
MVMGRWLPPFGIAFTADLTGALLSLAAAIVALAGAIHAGADIDASGRRYGFYPFLMLLMAGVTGAFLTGDLFNLYVWFEV
LLISSFGLIVLGSTREQIDGALKYAILNLIGTTLFLITVGYLYAIFGTLNMADIALKATELRGTAPLMTLASLFALAFAM
KAAAFPVNFWLPASYHTPRIVVSALFGGLLTKVGIYSLLRVMVMLFPVEREELSIVIAISAALTMVLGAMGALAQNDIRR
MLGYIVISGIGYMMAGIAIGTPSGVSGAIFYALHSMVLMTALYLAAGHAARLGGGFSLTSLGDLYRQAPWFSALALALFF
AGSGLPPFSGFWPKAVLVKSAIDIGAWWLAAAILVSGFIATIAFGRVFLLCFWRPVTTSAGQPALQPAARPPAPSVAPLV
GLTLLVVFFGLFPESLLNLSQQAAAGLGNPQAYIQSVFPQGGKP

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=227, Percent_Identity=25.1101321585903, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788613, Length=345, Percent_Identity=27.2463768115942, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788827, Length=354, Percent_Identity=26.271186440678, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788829, Length=370, Percent_Identity=25.1351351351351, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693160, Length=392, Percent_Identity=25.5102040816327, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788831, Length=305, Percent_Identity=25.9016393442623, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788614, Length=418, Percent_Identity=25.3588516746411, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI2367154, Length=315, Percent_Identity=23.4920634920635, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 55122; Mature: 54990

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSTTSTVTDLSAALVMAPVPLADWLIILPIALCIGAGAVLMMMRHAIRHHAAVAIAAL
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLVILNAALLWKVATQGPFTMVMGRWLPPFGIAFTADLTGALLSLAAAIVALAGAIHAG
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
ADIDASGRRYGFYPFLMLLMAGVTGAFLTGDLFNLYVWFEVLLISSFGLIVLGSTREQID
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHH
GALKYAILNLIGTTLFLITVGYLYAIFGTLNMADIALKATELRGTAPLMTLASLFALAFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MKAAAFPVNFWLPASYHTPRIVVSALFGGLLTKVGIYSLLRVMVMLFPVEREELSIVIAI
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEHH
SAALTMVLGAMGALAQNDIRRMLGYIVISGIGYMMAGIAIGTPSGVSGAIFYALHSMVLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TALYLAAGHAARLGGGFSLTSLGDLYRQAPWFSALALALFFAGSGLPPFSGFWPKAVLVK
HHHHHHHCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
SAIDIGAWWLAAAILVSGFIATIAFGRVFLLCFWRPVTTSAGQPALQPAARPPAPSVAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
VGLTLLVVFFGLFPESLLNLSQQAAAGLGNPQAYIQSVFPQGGKP
HHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ASSTTSTVTDLSAALVMAPVPLADWLIILPIALCIGAGAVLMMMRHAIRHHAAVAIAAL
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLVILNAALLWKVATQGPFTMVMGRWLPPFGIAFTADLTGALLSLAAAIVALAGAIHAG
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
ADIDASGRRYGFYPFLMLLMAGVTGAFLTGDLFNLYVWFEVLLISSFGLIVLGSTREQID
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHH
GALKYAILNLIGTTLFLITVGYLYAIFGTLNMADIALKATELRGTAPLMTLASLFALAFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MKAAAFPVNFWLPASYHTPRIVVSALFGGLLTKVGIYSLLRVMVMLFPVEREELSIVIAI
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEHH
SAALTMVLGAMGALAQNDIRRMLGYIVISGIGYMMAGIAIGTPSGVSGAIFYALHSMVLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TALYLAAGHAARLGGGFSLTSLGDLYRQAPWFSALALALFFAGSGLPPFSGFWPKAVLVK
HHHHHHHCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
SAIDIGAWWLAAAILVSGFIATIAFGRVFLLCFWRPVTTSAGQPALQPAARPPAPSVAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
VGLTLLVVFFGLFPESLLNLSQQAAAGLGNPQAYIQSVFPQGGKP
HHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]