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The map label for this gene is mnhA [H]

Identifier: 15888247

GI number: 15888247

Start: 898920

End: 901289

Strand: Direct

Name: mnhA [H]

Synonym: Atu0909

Alternate gene names: 15888247

Gene position: 898920-901289 (Clockwise)

Preceding gene: 15888245

Following gene: 15888248

Centisome position: 31.63

GC content: 62.57

Gene sequence:

>2370_bases
ATGACATCAGATGTCACGGCGCTGACATTCCTGTCGCTGTTTCTGCCGTTTCTGGCAGCGCTTGCCGCGCCGGCCCTCGT
GAAAAGGTTCGGCCATAATGCCGCATGGATCATCGCGATCGCGCCGGCTTTCGCCTTCGTGCATTTCGCGCTGATGCTGC
CGCAGATTGCGGCGGGCGGCGTGGTGACGGGCGGTTATGCCTGGGTGCCGAGCTTCAATCTCAGCTTCTCCTGGTTCATC
GATGGCCTGTCGCTCACCTTCGCGTTGCTGATAACAGGCATTGGCGTGCTGATCGTGCTTTATGCCGGCGGTTACATGAA
GGGGCATCCGCAGCAGGGCCGGTTCCTGTCCTTCCTGCTGCTGTTCATGGGCGCGATGCTGGGCGTCGTCGTCTCCGACA
GCGTGCTGATGCTGTTCGTGTTCTGGGAACTCACCTCAATCACGTCCTTCCTGCTGATCGGCTTCGACCACGAGCGCGCC
GCCTCGCGCCGGGCTGCGCTACAGGCGCTGGTGGTGACGGGCGGTGGTGGGCTGCTGCTGCTTGCTGGGCTGATCTTCAT
CTGGGACATCAGTGGTTTGACGCAATTGTCGATGCTGGTGCGCGGCGGCGATATTCTGCGCGACAGCCCGTTTTATCTGG
CGGCGCTGCTTCTGGTCCTTGGCGGTGCCTTCACCAAATCGGCGCAGTTTCCCTTCCATTTCTGGCTACCCAACGCCATG
GAAGCGCCCACGCCGGTCTCGGCCTATCTGCATTCGGCAACCATGGTTAAGGCCGGCGTCTATCTCCTGATGAGGCTCAA
CCCGGTTCTCGGCGATACTGCCGCCTGGCAGATCCTGCTGCCCTTCTTCGGCGGCCTGACCATGCTCACCGGCGCGCTGC
TCGCCGTGCGCCAGACCGATCTGAAGCTGATGCTTGCCTATACCACCGTCTCCTCGCTCGGCCTGCTCGTCATGCTCACG
GGCTTCGGCTCGGACCACGCCATCGAGGCGGCGGTGCTTTATCTGGTGGCGCATTCGCTGTTCAAGGGCGCCTTGTTCAT
GGTCGCTGGCATCATCGATCACGAGACCGGCACCCGCGATGTCACGAAGCTCGCCGGCTTGCGCAAGGCCATGCCCATCA
CCTTCGCCGCCGCACTTGCCGCGGCCATCTCCATGGCCGGCCTGCCGCCATTTTTCGGCTTCCTCGCCAAGGAAGAGATT
TATTATGCGCTGGCGCATGGCAATCCGCGCGCCGTGCTGTTCACCGGCATTGCCATTCTCGGCAACGGGCTGATGTTCGC
CGTCGCCTTCGCCGTGGGCTTGAAACCCTTCCTCGGCAAGCCGGTGAAAACGCCGAAAAACGCGCATGAGGGACCGCTGC
TGCTCTGGCTCGGTCCGGCGCTTTTGGCGCTGAAGGGGTTCACCATAGCGCTCTTTGCCGGCATCGCGCATTTTTACATC
TCCACCCCCATGGCAAGCGCGATTGCGGGTGAGCCGCGCCCGGTGGAAATCTCGCTCATTCCCCATATCGGCGTGCCGCT
TGGCCTGTCGCTGCTGACGATCGCGCTCGGCATTACCCTTTACACCCAGCTTTCAAGGGTGCGCAGCCTGATGGACCGCA
GCTTCAAGGCGCTCGGGGCCGGGCCGGACAGGGGCTTTGACGTCTTCATCGAAACGCTGGTGAGGATGTCCTTCCACGTC
ACGCGGCTTATCCAGCCCGGCCGGCTGGAGTTTTACGTCACCGCCACCTTCGCCGTCATCGCCGCCGTACTGCTGGTGCC
GCTGTTCCTCTATGACGAACTGCCGTCGATCCCGGCCTGGCCGCATGATATGCCCATCCATGAGCTGACCTTCATCGTCA
TCGCGGTGGCCGGTCTCCTCGCGGTGCTGACGGCATCGAGCCGGCTGACGGCCATCATCGCGCTCGGCATACAGGGTTTT
GCGGTGGCGGTCATCTTCCTGCTGTTCGGCGCGCCTGATCTCTCCTTCACGCAATTCATGGTCGAGACGCTCTCGGTGGT
CATCCTGACGCTCGTCATGACGCGGCTGCGCCTGTCCCCGTCGGACCATCGCGGGCTTGGCCAGAAACTGCTCGATAGCA
CCATCGCCATTGCCTGCGGAACCGGCTTTGCCCTGTTCCTGATGCGGGCGACGCAAGCGAGCTTCGACAACCGCCTGACC
GACTTCTACAATACCTATTCCAAGGTCATCGCCCACGGCGCGAATGTGGTGAATGTCATCATCGTCGATTTCCGCGGCAC
GGATACGCTCGGCGAAATCGCCGTCGTGATGATAACCGGTCTTGCCATCCTCGCGCTCATCCGCATCCGCCCCGCCGCCG
TCCTCAAGGGGCCGGCGAAGACGGTGAAAAAGAAGGGAGCGCGGACATGA

Upstream 100 bases:

>100_bases
TGGGTTGAGATGACCGTGCCTATGCCTTAACTGAAAAATCGATTTGAAAAAGACGCCGTTCATTGGCGCGCGGGGATGGG
GGACATCCCAGGAGATGTGA

Downstream 100 bases:

>100_bases
ACACGCTCATCCTGCGCACGGTCGCGCCTGTCGTGACCAGCCTCATGGTGCTGTTTTCCATCTTCGTTCTGCTGCGGGGC
CATAACGAGCCGGGCGGTGG

Product: putative monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 789; Mature: 788

Protein sequence:

>789_residues
MTSDVTALTFLSLFLPFLAALAAPALVKRFGHNAAWIIAIAPAFAFVHFALMLPQIAAGGVVTGGYAWVPSFNLSFSWFI
DGLSLTFALLITGIGVLIVLYAGGYMKGHPQQGRFLSFLLLFMGAMLGVVVSDSVLMLFVFWELTSITSFLLIGFDHERA
ASRRAALQALVVTGGGGLLLLAGLIFIWDISGLTQLSMLVRGGDILRDSPFYLAALLLVLGGAFTKSAQFPFHFWLPNAM
EAPTPVSAYLHSATMVKAGVYLLMRLNPVLGDTAAWQILLPFFGGLTMLTGALLAVRQTDLKLMLAYTTVSSLGLLVMLT
GFGSDHAIEAAVLYLVAHSLFKGALFMVAGIIDHETGTRDVTKLAGLRKAMPITFAAALAAAISMAGLPPFFGFLAKEEI
YYALAHGNPRAVLFTGIAILGNGLMFAVAFAVGLKPFLGKPVKTPKNAHEGPLLLWLGPALLALKGFTIALFAGIAHFYI
STPMASAIAGEPRPVEISLIPHIGVPLGLSLLTIALGITLYTQLSRVRSLMDRSFKALGAGPDRGFDVFIETLVRMSFHV
TRLIQPGRLEFYVTATFAVIAAVLLVPLFLYDELPSIPAWPHDMPIHELTFIVIAVAGLLAVLTASSRLTAIIALGIQGF
AVAVIFLLFGAPDLSFTQFMVETLSVVILTLVMTRLRLSPSDHRGLGQKLLDSTIAIACGTGFALFLMRATQASFDNRLT
DFYNTYSKVIAHGANVVNVIIVDFRGTDTLGEIAVVMITGLAILALIRIRPAAVLKGPAKTVKKKGART

Sequences:

>Translated_789_residues
MTSDVTALTFLSLFLPFLAALAAPALVKRFGHNAAWIIAIAPAFAFVHFALMLPQIAAGGVVTGGYAWVPSFNLSFSWFI
DGLSLTFALLITGIGVLIVLYAGGYMKGHPQQGRFLSFLLLFMGAMLGVVVSDSVLMLFVFWELTSITSFLLIGFDHERA
ASRRAALQALVVTGGGGLLLLAGLIFIWDISGLTQLSMLVRGGDILRDSPFYLAALLLVLGGAFTKSAQFPFHFWLPNAM
EAPTPVSAYLHSATMVKAGVYLLMRLNPVLGDTAAWQILLPFFGGLTMLTGALLAVRQTDLKLMLAYTTVSSLGLLVMLT
GFGSDHAIEAAVLYLVAHSLFKGALFMVAGIIDHETGTRDVTKLAGLRKAMPITFAAALAAAISMAGLPPFFGFLAKEEI
YYALAHGNPRAVLFTGIAILGNGLMFAVAFAVGLKPFLGKPVKTPKNAHEGPLLLWLGPALLALKGFTIALFAGIAHFYI
STPMASAIAGEPRPVEISLIPHIGVPLGLSLLTIALGITLYTQLSRVRSLMDRSFKALGAGPDRGFDVFIETLVRMSFHV
TRLIQPGRLEFYVTATFAVIAAVLLVPLFLYDELPSIPAWPHDMPIHELTFIVIAVAGLLAVLTASSRLTAIIALGIQGF
AVAVIFLLFGAPDLSFTQFMVETLSVVILTLVMTRLRLSPSDHRGLGQKLLDSTIAIACGTGFALFLMRATQASFDNRLT
DFYNTYSKVIAHGANVVNVIIVDFRGTDTLGEIAVVMITGLAILALIRIRPAAVLKGPAKTVKKKGART
>Mature_788_residues
TSDVTALTFLSLFLPFLAALAAPALVKRFGHNAAWIIAIAPAFAFVHFALMLPQIAAGGVVTGGYAWVPSFNLSFSWFID
GLSLTFALLITGIGVLIVLYAGGYMKGHPQQGRFLSFLLLFMGAMLGVVVSDSVLMLFVFWELTSITSFLLIGFDHERAA
SRRAALQALVVTGGGGLLLLAGLIFIWDISGLTQLSMLVRGGDILRDSPFYLAALLLVLGGAFTKSAQFPFHFWLPNAME
APTPVSAYLHSATMVKAGVYLLMRLNPVLGDTAAWQILLPFFGGLTMLTGALLAVRQTDLKLMLAYTTVSSLGLLVMLTG
FGSDHAIEAAVLYLVAHSLFKGALFMVAGIIDHETGTRDVTKLAGLRKAMPITFAAALAAAISMAGLPPFFGFLAKEEIY
YALAHGNPRAVLFTGIAILGNGLMFAVAFAVGLKPFLGKPVKTPKNAHEGPLLLWLGPALLALKGFTIALFAGIAHFYIS
TPMASAIAGEPRPVEISLIPHIGVPLGLSLLTIALGITLYTQLSRVRSLMDRSFKALGAGPDRGFDVFIETLVRMSFHVT
RLIQPGRLEFYVTATFAVIAAVLLVPLFLYDELPSIPAWPHDMPIHELTFIVIAVAGLLAVLTASSRLTAIIALGIQGFA
VAVIFLLFGAPDLSFTQFMVETLSVVILTLVMTRLRLSPSDHRGLGQKLLDSTIAIACGTGFALFLMRATQASFDNRLTD
FYNTYSKVIAHGANVVNVIIVDFRGTDTLGEIAVVMITGLAILALIRIRPAAVLKGPAKTVKKKGART

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=394, Percent_Identity=33.248730964467, Blast_Score=182, Evalue=9e-46,
Organism=Homo sapiens, GI251831116, Length=377, Percent_Identity=25.4641909814324, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1788614, Length=353, Percent_Identity=35.6940509915014, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1788829, Length=354, Percent_Identity=35.3107344632768, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1788831, Length=337, Percent_Identity=32.0474777448071, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1788827, Length=438, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788613, Length=402, Percent_Identity=27.363184079602, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI145693160, Length=386, Percent_Identity=27.2020725388601, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI2367154, Length=288, Percent_Identity=31.5972222222222, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 84325; Mature: 84194

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDVTALTFLSLFLPFLAALAAPALVKRFGHNAAWIIAIAPAFAFVHFALMLPQIAAGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
VVTGGYAWVPSFNLSFSWFIDGLSLTFALLITGIGVLIVLYAGGYMKGHPQQGRFLSFLL
EEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LFMGAMLGVVVSDSVLMLFVFWELTSITSFLLIGFDHERAASRRAALQALVVTGGGGLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCHHHH
LAGLIFIWDISGLTQLSMLVRGGDILRDSPFYLAALLLVLGGAFTKSAQFPFHFWLPNAM
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
EAPTPVSAYLHSATMVKAGVYLLMRLNPVLGDTAAWQILLPFFGGLTMLTGALLAVRQTD
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
LKLMLAYTTVSSLGLLVMLTGFGSDHAIEAAVLYLVAHSLFKGALFMVAGIIDHETGTRD
HHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VTKLAGLRKAMPITFAAALAAAISMAGLPPFFGFLAKEEIYYALAHGNPRAVLFTGIAIL
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEECCCCCEEHEEHHHHH
GNGLMFAVAFAVGLKPFLGKPVKTPKNAHEGPLLLWLGPALLALKGFTIALFAGIAHFYI
HCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
STPMASAIAGEPRPVEISLIPHIGVPLGLSLLTIALGITLYTQLSRVRSLMDRSFKALGA
HCCHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPDRGFDVFIETLVRMSFHVTRLIQPGRLEFYVTATFAVIAAVLLVPLFLYDELPSIPAW
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCC
PHDMPIHELTFIVIAVAGLLAVLTASSRLTAIIALGIQGFAVAVIFLLFGAPDLSFTQFM
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VETLSVVILTLVMTRLRLSPSDHRGLGQKLLDSTIAIACGTGFALFLMRATQASFDNRLT
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHH
DFYNTYSKVIAHGANVVNVIIVDFRGTDTLGEIAVVMITGLAILALIRIRPAAVLKGPAK
HHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHH
TVKKKGART
HHHHCCCCC
>Mature Secondary Structure 
TSDVTALTFLSLFLPFLAALAAPALVKRFGHNAAWIIAIAPAFAFVHFALMLPQIAAGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
VVTGGYAWVPSFNLSFSWFIDGLSLTFALLITGIGVLIVLYAGGYMKGHPQQGRFLSFLL
EEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LFMGAMLGVVVSDSVLMLFVFWELTSITSFLLIGFDHERAASRRAALQALVVTGGGGLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCHHHH
LAGLIFIWDISGLTQLSMLVRGGDILRDSPFYLAALLLVLGGAFTKSAQFPFHFWLPNAM
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
EAPTPVSAYLHSATMVKAGVYLLMRLNPVLGDTAAWQILLPFFGGLTMLTGALLAVRQTD
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
LKLMLAYTTVSSLGLLVMLTGFGSDHAIEAAVLYLVAHSLFKGALFMVAGIIDHETGTRD
HHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VTKLAGLRKAMPITFAAALAAAISMAGLPPFFGFLAKEEIYYALAHGNPRAVLFTGIAIL
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEECCCCCEEHEEHHHHH
GNGLMFAVAFAVGLKPFLGKPVKTPKNAHEGPLLLWLGPALLALKGFTIALFAGIAHFYI
HCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
STPMASAIAGEPRPVEISLIPHIGVPLGLSLLTIALGITLYTQLSRVRSLMDRSFKALGA
HCCHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPDRGFDVFIETLVRMSFHVTRLIQPGRLEFYVTATFAVIAAVLLVPLFLYDELPSIPAW
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCC
PHDMPIHELTFIVIAVAGLLAVLTASSRLTAIIALGIQGFAVAVIFLLFGAPDLSFTQFM
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VETLSVVILTLVMTRLRLSPSDHRGLGQKLLDSTIAIACGTGFALFLMRATQASFDNRLT
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHH
DFYNTYSKVIAHGANVVNVIIVDFRGTDTLGEIAVVMITGLAILALIRIRPAAVLKGPAK
HHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHH
TVKKKGART
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]