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The map label for this gene is dltE [H]

Identifier: 15888205

GI number: 15888205

Start: 861178

End: 861936

Strand: Direct

Name: dltE [H]

Synonym: Atu0864

Alternate gene names: 15888205

Gene position: 861178-861936 (Clockwise)

Preceding gene: 15888203

Following gene: 15888206

Centisome position: 30.31

GC content: 60.34

Gene sequence:

>759_bases
ATGAAAATCACCGGCAATACCATTCTCATCACCGGCGGCGGCTCCGGCATCGGTAGGGCGCTGGCGGAAGCGTTTCATCA
GGCCGGCAACAGGGTCATCATTTCCGGCCGCCGTCAGGCTGTGCTCGATGAGGTAACGGCCGCCAATCCCGGCATGGCCT
CAATGGTCATGGATGCCGGCGATGCCGAAGGCATCCGCGCTTTTGCCGAGGCGCTGGTAAAGGCCCATCCCACCCTCAAT
GCCGTCATCAACAATGCCGGCATCATGCGCCCGGAAGAAATCGCCGCCGCGCCTGACTATCTCGACACGGCGGAAGAAAC
CATTGCGACCAACCTCCTTGCCCCCATCCGTCTGACGGCGGCGCTTTTGCCGCATTTCCTCAAGCAGCCGGCCGCCACGG
TGCTGACGGTCTCCTCCGGCCTCGCTTTCGTGCCCATGGTGCTGACCCCCACTTACAGCGCCACCAAATCGGCCATCCAT
GCCTATTCGGTTGCGCTACGCGAGCAACTGAAGGAAACGTCCGTGGAGGTCATCGAAATCGTGCCGCCTTATGTGCAGAC
GACGCTGATGGGCGAAGGGCAGGCCAATGACGAAAGGGCCATGCCGCTTGAGGCCTTCATTTCCGAAGTGATGGATATTC
TCAAGAACCGCCCGGATGAGAAGGAAGTGGTGGTGGAACGCTGCAAGCCGCTGCGTTTCGCCGCGCAGAACGGCAATTTC
GATCAGGTCTTCGCCATGATCAACCATATGCACCCGTAA

Upstream 100 bases:

>100_bases
ACACTCTCCTTTGGGTAAGTATAAATCTTTTGGGTGCCTACTTTCGATTGGAGAGTATCATAGCTAACTTCCTTTCAGCC
AACAAGCAAAGGATGTTCCA

Downstream 100 bases:

>100_bases
ACAGCGCAACGTTTGTTTTGCAAGGACATCCTGTGATAGCGTCGCGCTCTTATCCGCGGAGCGTGCGCTTTCATGGGCCA
AGCCCAGTTCCTGATGCCGA

Product: oxidoreductase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKITGNTILITGGGSGIGRALAEAFHQAGNRVIISGRRQAVLDEVTAANPGMASMVMDAGDAEGIRAFAEALVKAHPTLN
AVINNAGIMRPEEIAAAPDYLDTAEETIATNLLAPIRLTAALLPHFLKQPAATVLTVSSGLAFVPMVLTPTYSATKSAIH
AYSVALREQLKETSVEVIEIVPPYVQTTLMGEGQANDERAMPLEAFISEVMDILKNRPDEKEVVVERCKPLRFAAQNGNF
DQVFAMINHMHP

Sequences:

>Translated_252_residues
MKITGNTILITGGGSGIGRALAEAFHQAGNRVIISGRRQAVLDEVTAANPGMASMVMDAGDAEGIRAFAEALVKAHPTLN
AVINNAGIMRPEEIAAAPDYLDTAEETIATNLLAPIRLTAALLPHFLKQPAATVLTVSSGLAFVPMVLTPTYSATKSAIH
AYSVALREQLKETSVEVIEIVPPYVQTTLMGEGQANDERAMPLEAFISEVMDILKNRPDEKEVVVERCKPLRFAAQNGNF
DQVFAMINHMHP
>Mature_252_residues
MKITGNTILITGGGSGIGRALAEAFHQAGNRVIISGRRQAVLDEVTAANPGMASMVMDAGDAEGIRAFAEALVKAHPTLN
AVINNAGIMRPEEIAAAPDYLDTAEETIATNLLAPIRLTAALLPHFLKQPAATVLTVSSGLAFVPMVLTPTYSATKSAIH
AYSVALREQLKETSVEVIEIVPPYVQTTLMGEGQANDERAMPLEAFISEVMDILKNRPDEKEVVVERCKPLRFAAQNGNF
DQVFAMINHMHP

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG3967

COG function: function code M; Short-chain dehydrogenase involved in D-alanine esterification of lipoteichoic acid and wall teichoic acid (D-alanine transfer protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI20149619, Length=192, Percent_Identity=28.125, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI7705855, Length=192, Percent_Identity=26.0416666666667, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI142976729, Length=195, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI4557649, Length=220, Percent_Identity=25, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI7706318, Length=254, Percent_Identity=22.8346456692913, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157739938, Length=211, Percent_Identity=21.8009478672986, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1786812, Length=191, Percent_Identity=29.3193717277487, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787820, Length=185, Percent_Identity=28.1081081081081, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787335, Length=204, Percent_Identity=25.4901960784314, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082160, Length=208, Percent_Identity=25, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789208, Length=205, Percent_Identity=21.9512195121951, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082100, Length=192, Percent_Identity=27.6041666666667, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI32563809, Length=230, Percent_Identity=26.9565217391304, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507613, Length=230, Percent_Identity=26.9565217391304, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17563726, Length=199, Percent_Identity=26.6331658291457, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=197, Percent_Identity=24.8730964467005, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17564282, Length=195, Percent_Identity=27.6923076923077, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=213, Percent_Identity=28.169014084507, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21358495, Length=196, Percent_Identity=28.0612244897959, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24580925, Length=204, Percent_Identity=25.4901960784314, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24643142, Length=207, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24651139, Length=193, Percent_Identity=30.5699481865285, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24647946, Length=146, Percent_Identity=32.1917808219178, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI23397609, Length=191, Percent_Identity=26.7015706806283, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 26985; Mature: 26985

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITGNTILITGGGSGIGRALAEAFHQAGNRVIISGRRQAVLDEVTAANPGMASMVMDAG
CEEECCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHCCC
DAEGIRAFAEALVKAHPTLNAVINNAGIMRPEEIAAAPDYLDTAEETIATNLLAPIRLTA
CHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ALLPHFLKQPAATVLTVSSGLAFVPMVLTPTYSATKSAIHAYSVALREQLKETSVEVIEI
HHHHHHHHCCHHEEEECCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPPYVQTTLMGEGQANDERAMPLEAFISEVMDILKNRPDEKEVVVERCKPLRFAAQNGNF
CCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCH
DQVFAMINHMHP
HHHHHHHHHCCC
>Mature Secondary Structure
MKITGNTILITGGGSGIGRALAEAFHQAGNRVIISGRRQAVLDEVTAANPGMASMVMDAG
CEEECCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHCCC
DAEGIRAFAEALVKAHPTLNAVINNAGIMRPEEIAAAPDYLDTAEETIATNLLAPIRLTA
CHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ALLPHFLKQPAATVLTVSSGLAFVPMVLTPTYSATKSAIHAYSVALREQLKETSVEVIEI
HHHHHHHHCCHHEEEECCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPPYVQTTLMGEGQANDERAMPLEAFISEVMDILKNRPDEKEVVVERCKPLRFAAQNGNF
CCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCH
DQVFAMINHMHP
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 7797557 [H]