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The map label for this gene is ispH

Identifier: 15888117

GI number: 15888117

Start: 769735

End: 770781

Strand: Direct

Name: ispH

Synonym: Atu0774

Alternate gene names: 15888117

Gene position: 769735-770781 (Clockwise)

Preceding gene: 159184460

Following gene: 15888118

Centisome position: 27.09

GC content: 60.65

Gene sequence:

>1047_bases
ATGAACATCGCCGTTTCCAAACCCCCTTTGACGATAAGGCTGTGCGGACCGCGCGGCTTTTGCGCGGGCGTGGATAGGGC
GATTCAGATCGTCGTGCTGGCGCTGAAAGCCTATGGCGCACCGGTCTATGTTCGCCACGAAATCGTGCATAATCGTTACG
TTGTTGAAGGCCTGGAAGCCAAGGGCGCGATTTTCGTCGAGGAACTGCATGAGATTCCTGCCGAGCATCGCGAGCAGCCG
GTGGTCTTTTCCGCCCACGGTGTGCCGAAATCCGTGCCTGAAGACGCGCAGGCACGTAACCTGTTTTATCTCGACGCGAC
TTGCCCGCTGGTCTCCAAGGTCCATAAGCAGGCTATGCGCCACCAGCGCCTCGGCCGTCATGTGGTGCTGATCGGCCATG
CCGGTCACCCCGAAGTCATTGGCACGATGGGCCAACTGCCCGAAGGTACGGTCTCGCTGGTCGAGACGGTGGAAGATGCG
GGTGTTTACGAACCGGTCGACCGTGAAAACCTCGGTTTCGTCACGCAGACGACACTGTCGGTCGATGATACCGCCGGCGT
CATCGCCAGGCTTCAGGAACGGTTTCCCGCCATTCAGGCGCCTGCCGCCGACAGCATCTGTTACGCAACCACCAACCGTC
AGGATGCGGTGAAGCAGGCAGCCCCCGGCTGCGATCTCTTCATCGTCGTTGGCGCCCCCAATTCCTCCAATTCAAAGCGG
CTGGTGGAAGTGGCGCTGCGCGCCGGCGCCAAACACTCGGTGCTGGTGCAGCGGGCGTCGGAAATCGACTGGAATGAGAT
CGGCGACATCCGCACGGTCGGCCTTTCGGCCGGTGCTTCCGCGCCGGAGGTCATCGTTGATGAGATCATCGAGGCCTTCA
AGGCACGTTTCGATACCACGCTCGATCTCGCCGTGACGGTTGAGGAAACGGAACATTTCCTCGTCAACCGCGAGCTGCGC
AGCATCGAGTTGACGACGGATGACATGGCCTTCGTTAATGGCAATGCCAGCAACGCGCTGCCGCCAAAAGCCACCGCCGG
TATCTGA

Upstream 100 bases:

>100_bases
GGTTTTGCCTATTTCCGGAACGGTAAGGCGCAGGGTGAATGAAGAGCGCGCCGTCCGGTAGCTTGCCGGGCAAGGCCAAG
GAGCGCGCCAAGGAGCACGA

Downstream 100 bases:

>100_bases
AGCGGGGTGGCGCTGAGCAAGGGCGGTTTTCGTCCTACCTTTGGCATGCTCCCCCTAGATTTCTAGTCCTTCCTTCATCA
AGAGATATAAAATTGGCAGT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNIAVSKPPLTIRLCGPRGFCAGVDRAIQIVVLALKAYGAPVYVRHEIVHNRYVVEGLEAKGAIFVEELHEIPAEHREQP
VVFSAHGVPKSVPEDAQARNLFYLDATCPLVSKVHKQAMRHQRLGRHVVLIGHAGHPEVIGTMGQLPEGTVSLVETVEDA
GVYEPVDRENLGFVTQTTLSVDDTAGVIARLQERFPAIQAPAADSICYATTNRQDAVKQAAPGCDLFIVVGAPNSSNSKR
LVEVALRAGAKHSVLVQRASEIDWNEIGDIRTVGLSAGASAPEVIVDEIIEAFKARFDTTLDLAVTVEETEHFLVNRELR
SIELTTDDMAFVNGNASNALPPKATAGI

Sequences:

>Translated_348_residues
MNIAVSKPPLTIRLCGPRGFCAGVDRAIQIVVLALKAYGAPVYVRHEIVHNRYVVEGLEAKGAIFVEELHEIPAEHREQP
VVFSAHGVPKSVPEDAQARNLFYLDATCPLVSKVHKQAMRHQRLGRHVVLIGHAGHPEVIGTMGQLPEGTVSLVETVEDA
GVYEPVDRENLGFVTQTTLSVDDTAGVIARLQERFPAIQAPAADSICYATTNRQDAVKQAAPGCDLFIVVGAPNSSNSKR
LVEVALRAGAKHSVLVQRASEIDWNEIGDIRTVGLSAGASAPEVIVDEIIEAFKARFDTTLDLAVTVEETEHFLVNRELR
SIELTTDDMAFVNGNASNALPPKATAGI
>Mature_348_residues
MNIAVSKPPLTIRLCGPRGFCAGVDRAIQIVVLALKAYGAPVYVRHEIVHNRYVVEGLEAKGAIFVEELHEIPAEHREQP
VVFSAHGVPKSVPEDAQARNLFYLDATCPLVSKVHKQAMRHQRLGRHVVLIGHAGHPEVIGTMGQLPEGTVSLVETVEDA
GVYEPVDRENLGFVTQTTLSVDDTAGVIARLQERFPAIQAPAADSICYATTNRQDAVKQAAPGCDLFIVVGAPNSSNSKR
LVEVALRAGAKHSVLVQRASEIDWNEIGDIRTVGLSAGASAPEVIVDEIIEAFKARFDTTLDLAVTVEETEHFLVNRELR
SIELTTDDMAFVNGNASNALPPKATAGI

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=283, Percent_Identity=52.2968197879859, Blast_Score=291, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_AGRT5 (P58673)

Other databases:

- EMBL:   AE007869
- PIR:   AH2671
- PIR:   F97453
- RefSeq:   NP_353798.1
- STRING:   P58673
- GeneID:   1132812
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0774
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   WGAPVYV
- PhylomeDB:   P58673
- ProtClustDB:   PRK01045
- BioCyc:   ATUM176299-1:ATU0774-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 37538; Mature: 37538

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAVSKPPLTIRLCGPRGFCAGVDRAIQIVVLALKAYGAPVYVRHEIVHNRYVVEGLEA
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHEEECCCC
KGAIFVEELHEIPAEHREQPVVFSAHGVPKSVPEDAQARNLFYLDATCPLVSKVHKQAMR
CCCHHHHHHHHCCHHHCCCCEEEEECCCCCCCCCCCHHCEEEEEECCCHHHHHHHHHHHH
HQRLGRHVVLIGHAGHPEVIGTMGQLPEGTVSLVETVEDAGVYEPVDRENLGFVTQTTLS
HHHCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEC
VDDTAGVIARLQERFPAIQAPAADSICYATTNRQDAVKQAAPGCDLFIVVGAPNSSNSKR
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCHH
LVEVALRAGAKHSVLVQRASEIDWNEIGDIRTVGLSAGASAPEVIVDEIIEAFKARFDTT
HHHHHHHCCCCHHHHHHHHHCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCE
LDLAVTVEETEHFLVNRELRSIELTTDDMAFVNGNASNALPPKATAGI
EEEEEEEECHHHHHHCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIAVSKPPLTIRLCGPRGFCAGVDRAIQIVVLALKAYGAPVYVRHEIVHNRYVVEGLEA
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHEEECCCC
KGAIFVEELHEIPAEHREQPVVFSAHGVPKSVPEDAQARNLFYLDATCPLVSKVHKQAMR
CCCHHHHHHHHCCHHHCCCCEEEEECCCCCCCCCCCHHCEEEEEECCCHHHHHHHHHHHH
HQRLGRHVVLIGHAGHPEVIGTMGQLPEGTVSLVETVEDAGVYEPVDRENLGFVTQTTLS
HHHCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEC
VDDTAGVIARLQERFPAIQAPAADSICYATTNRQDAVKQAAPGCDLFIVVGAPNSSNSKR
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCHH
LVEVALRAGAKHSVLVQRASEIDWNEIGDIRTVGLSAGASAPEVIVDEIIEAFKARFDTT
HHHHHHHCCCCHHHHHHHHHCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCE
LDLAVTVEETEHFLVNRELRSIELTTDDMAFVNGNASNALPPKATAGI
EEEEEEEECHHHHHHCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194