Click here to switch to the map view.

The map label for this gene is glcF [H]

Identifier: 15888009

GI number: 15888009

Start: 663091

End: 664392

Strand: Direct

Name: glcF [H]

Synonym: Atu0667

Alternate gene names: 15888009

Gene position: 663091-664392 (Clockwise)

Preceding gene: 159184413

Following gene: 159184415

Centisome position: 23.34

GC content: 62.52

Gene sequence:

>1302_bases
ATGCAAACATCCTTCACATCAGAACAGCTGGCCGATCCGCATGTGGCGGAATCCGAAAAAATCCTGCGCAAATGTGTGCA
TTGCGGTTTCTGCACTGCCACCTGTCCCACCTATGTCACGCTTGGCAATGAGCTGGACAGCCCGCGCGGCCGCATCTACC
TCATCAAGGACATGCTGGAAAACAGCCGCCCGGCCGACCGCGAAGTGGTCACCCATATCGACCGCTGTCTCTCCTGCCTT
GCCTGCACCACTACCTGTCCCTCCGGCGTGGATTATATGCATCTGGTGGATCACGCGCGCGCCCACATCGAACAGACATA
CAAGCGCCCCTCCATGGACCGGCTGATCCGCAATCTTCTGGTTGCCGTCCTGCCGCATCCCGGCCGCTTCCGGCTGGCTC
TGCATCTGGCAAGGCTGGCGCGGCCCTTCTCGGGTCTGCTTGCCAAGTCTCCGGCGCTGAAGCCGCTGCAATCCATGCTC
GCTCTTGCCCCCGCTGCGGTGCCGCCGGCCTCCGCATCCGCCCGTCCGGGCGCACGCCCAGCGGAAGGCGAAAAGCGCGG
GCGCGTTGCCCTCCTCACCGGCTGCGCCCAGCCGGTGCTCGACCCTGGAATCAACGAGGCGACGCTGCGTCTGCTCGCCC
GGCTCGGCATCGAGGTCGTGGTGCCAAGAGGCGAGGGCTGCTGCGGTTCGCTGGTGCATCACATGGGCCGTGAGGAAGAG
GCACTCGCCTCCGCCCGGCGGAATGTCGATGTCTGGATGCGGGAGATGGAAGAGGGCGGTCTCGACGCCATCATCATCAC
CGCGTCCGGCTGCGGCACCACCATCAAGGATTATGGCCATATGCTGCGGCTCGATCCAGCCTATGCCGAAAAAGCCGCGC
GTGTCTCGGCGCTTGCGAAGGACATCACCGAATATCTCGCCAGCCTCGATCTGCCGCAAAGGCAGAGCCAAGGGCTGACG
GTCGCCTATCATTCCGCCTGCTCCATGCAGCATGGCCAGAAGATTACGCTGGCGCCGAAACAGCTCTTGAAAGCGGCAGG
TTTCACGGTGCGTGATCCGGCGGAAGGGCATCTGTGCTGCGGCTCGGCTGGAACCTACAACATCCTGCAGCCGGAAATTT
CGAGCAAGCTCAAGGCGCGCAAGGTGAAAAATATCGAAGCCACAAGGGCGGATGTCATTGCGACAGGCAATATCGGCTGC
ATCACGCAGATCGGAACCGGTACGGATATGCCGATCCTGCACACGGTGGAACTGCTGGACTGGGCTTATGGCGGGCCGAA
GCCGGCGAGGCTTTTGGTTTAA

Upstream 100 bases:

>100_bases
AGAAGCGCTGCTTTCGGCGCGTATCAGGGAGAAGCTCGATCCGGCGGGCATCTTCAACCCCGGCAAGATGGCTGTAACGG
TATAAGGGGCGGTCTGAACC

Downstream 100 bases:

>100_bases
AACTTATACGCCACGCCGAAATTCACCGCATTGGTGATGACGTCGGTCTTCAACGAGCCGCCATTGTCGATATCCACGTC
GATAAAGGAATAGGTGCCCT

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MQTSFTSEQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLENSRPADREVVTHIDRCLSCL
ACTTTCPSGVDYMHLVDHARAHIEQTYKRPSMDRLIRNLLVAVLPHPGRFRLALHLARLARPFSGLLAKSPALKPLQSML
ALAPAAVPPASASARPGARPAEGEKRGRVALLTGCAQPVLDPGINEATLRLLARLGIEVVVPRGEGCCGSLVHHMGREEE
ALASARRNVDVWMREMEEGGLDAIIITASGCGTTIKDYGHMLRLDPAYAEKAARVSALAKDITEYLASLDLPQRQSQGLT
VAYHSACSMQHGQKITLAPKQLLKAAGFTVRDPAEGHLCCGSAGTYNILQPEISSKLKARKVKNIEATRADVIATGNIGC
ITQIGTGTDMPILHTVELLDWAYGGPKPARLLV

Sequences:

>Translated_433_residues
MQTSFTSEQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLENSRPADREVVTHIDRCLSCL
ACTTTCPSGVDYMHLVDHARAHIEQTYKRPSMDRLIRNLLVAVLPHPGRFRLALHLARLARPFSGLLAKSPALKPLQSML
ALAPAAVPPASASARPGARPAEGEKRGRVALLTGCAQPVLDPGINEATLRLLARLGIEVVVPRGEGCCGSLVHHMGREEE
ALASARRNVDVWMREMEEGGLDAIIITASGCGTTIKDYGHMLRLDPAYAEKAARVSALAKDITEYLASLDLPQRQSQGLT
VAYHSACSMQHGQKITLAPKQLLKAAGFTVRDPAEGHLCCGSAGTYNILQPEISSKLKARKVKNIEATRADVIATGNIGC
ITQIGTGTDMPILHTVELLDWAYGGPKPARLLV
>Mature_433_residues
MQTSFTSEQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLENSRPADREVVTHIDRCLSCL
ACTTTCPSGVDYMHLVDHARAHIEQTYKRPSMDRLIRNLLVAVLPHPGRFRLALHLARLARPFSGLLAKSPALKPLQSML
ALAPAAVPPASASARPGARPAEGEKRGRVALLTGCAQPVLDPGINEATLRLLARLGIEVVVPRGEGCCGSLVHHMGREEE
ALASARRNVDVWMREMEEGGLDAIIITASGCGTTIKDYGHMLRLDPAYAEKAARVSALAKDITEYLASLDLPQRQSQGLT
VAYHSACSMQHGQKITLAPKQLLKAAGFTVRDPAEGHLCCGSAGTYNILQPEISSKLKARKVKNIEATRADVIATGNIGC
ITQIGTGTDMPILHTVELLDWAYGGPKPARLLV

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=423, Percent_Identity=42.789598108747, Blast_Score=334, Evalue=5e-93,
Organism=Escherichia coli, GI1786497, Length=242, Percent_Identity=26.8595041322314, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788576, Length=423, Percent_Identity=25.531914893617, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 46767; Mature: 46767

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSFTSEQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLE
CCCCCCHHHHCCCCCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCCEEEEEHHHHC
NSRPADREVVTHIDRCLSCLACTTTCPSGVDYMHLVDHARAHIEQTYKRPSMDRLIRNLL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VAVLPHPGRFRLALHLARLARPFSGLLAKSPALKPLQSMLALAPAAVPPASASARPGARP
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AEGEKRGRVALLTGCAQPVLDPGINEATLRLLARLGIEVVVPRGEGCCGSLVHHMGREEE
CCCCCCCCEEEEECCCHHHCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCHHH
ALASARRNVDVWMREMEEGGLDAIIITASGCGTTIKDYGHMLRLDPAYAEKAARVSALAK
HHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCEEEECHHHHHHHHHHHHHHH
DITEYLASLDLPQRQSQGLTVAYHSACSMQHGQKITLAPKQLLKAAGFTVRDPAEGHLCC
HHHHHHHHCCCCCHHCCCCEEEEHHHHHHCCCCEEEECHHHHHHHCCCEECCCCCCCEEE
GSAGTYNILQPEISSKLKARKVKNIEATRADVIATGNIGCITQIGTGTDMPILHTVELLD
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCEEEECCCCEEEEECCCCCCHHHHHHHHHH
WAYGGPKPARLLV
HHCCCCCCCCCCC
>Mature Secondary Structure
MQTSFTSEQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLE
CCCCCCHHHHCCCCCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCCEEEEEHHHHC
NSRPADREVVTHIDRCLSCLACTTTCPSGVDYMHLVDHARAHIEQTYKRPSMDRLIRNLL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VAVLPHPGRFRLALHLARLARPFSGLLAKSPALKPLQSMLALAPAAVPPASASARPGARP
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AEGEKRGRVALLTGCAQPVLDPGINEATLRLLARLGIEVVVPRGEGCCGSLVHHMGREEE
CCCCCCCCEEEEECCCHHHCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCHHH
ALASARRNVDVWMREMEEGGLDAIIITASGCGTTIKDYGHMLRLDPAYAEKAARVSALAK
HHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCEEEECHHHHHHHHHHHHHHH
DITEYLASLDLPQRQSQGLTVAYHSACSMQHGQKITLAPKQLLKAAGFTVRDPAEGHLCC
HHHHHHHHCCCCCHHCCCCEEEEHHHHHHCCCCEEEECHHHHHHHCCCEECCCCCCCEEE
GSAGTYNILQPEISSKLKARKVKNIEATRADVIATGNIGCITQIGTGTDMPILHTVELLD
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCEEEECCCCEEEEECCCCCCHHHHHHHHHH
WAYGGPKPARLLV
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]