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The map label for this gene is potF [H]

Identifier: 15887954

GI number: 15887954

Start: 596390

End: 597487

Strand: Direct

Name: potF [H]

Synonym: Atu0609

Alternate gene names: 15887954

Gene position: 596390-597487 (Clockwise)

Preceding gene: 15887953

Following gene: 15887955

Centisome position: 20.99

GC content: 55.83

Gene sequence:

>1098_bases
ATGAAAAACCGTTCTCTCCGCCTGACCTTGGCTCTCACCACCGCGCTCGTTGCAGCAGGCTCGGCAATGGCCCAGGAGAA
GGTCGTCCACGTCTATAACTGGTCGGACTATATCGATCCCGTCATCCTTGAAGATTTTACCAAGGAAACCGGGATAAAGG
TGGTCTACGACGTGTATGACGGCAACGAAGTGCTGGAGACCAAGCTTTTGGCCGGCAGCTCGGGTTATGACGTCGTGGCG
CCGACATCGCCTTTCCTGGCGCGGCAGATCAAGGCCGGTGTCTACCAGAAGCTGGACAAGTCCAAGCTGCCGAACCTCAA
GAATGCGTGGCCGGACATTACCGAACGCCTGGCGAAATATGACCCCGGCAACGAATATGCCGTCAACTACATGTGGGGCA
CCACCGGCATCGGCTACAATGTCGCCAAGGTGAAGGCCGCGCTCGGCGATGTGCCTGTTGATAGCTGGGACGTGTTGTTC
AAGCCGGAAAATGCCGAGAAGCTGAAATCCTGTGGTATCAACATTCTCGACGCGTCGGACGAGACTTTCGCGATTGCGAT
GAACTATCTCGGCAAGAATCCCGACAGTAAGGAAACGGCCGACCTGGAAGCGGGCGGCGAGGTCTATTCGAAGATTCGCC
CCTATGTGAAGACCTTCAATTCATCGCCCTATATCAATGATCTCGCCAATGGCGACACTTGCATCTCCATCGGCTGGTCC
GGCGATATTCTTCAGGCAAAAACGCGCGCCGAGGAAGCCAAGAACGGCGTTGAGGTGAATTATGTCATCCCCAAGGAAGG
CACCTATATCTGGATGGATAGCTTTGCCATTCCGGCTGATGCCAAGAATGTCGATGAGGCCCATGCCTTCATCAACTACA
TGATGAAGCCTGAAGTCGCCGCCAAGGCTTCCGACTACGTGCAATACGCCAACGGCAACCTTCCCTCGCAGGCGCTGATG
GATCCGTCGGTCGCGAAGAACCCGGGGGTCTATCCCGATCCGGAAACGATGAAGAAGCTCTTCACCATTTCGCCCTACGG
ACCGAAGGAGCAGCGTGTGTTGAACCGCGTCTGGACGCAGATAAAGACCGGCACCTGA

Upstream 100 bases:

>100_bases
TGCTGCACATTTCCCGCTTGCTCCCTTTTGTTTTCCTTTCTAGTCTCGCTAAAAAAGGGGATCACGCTTCCGTCGTGAGG
ATGCGTTAACAGGAGACATC

Downstream 100 bases:

>100_bases
TCGGACTTGGGAGAGGGTGGCGCTGTCCACCCTCTTTCTCCTTGATGTCGACGATGGATTCTTGGGTGAAATGGGGCGGG
CATGGCAAAGACACTCGGAC

Product: ABC transporter, substrate binding protein (putrescine)

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKNRSLRLTLALTTALVAAGSAMAQEKVVHVYNWSDYIDPVILEDFTKETGIKVVYDVYDGNEVLETKLLAGSSGYDVVA
PTSPFLARQIKAGVYQKLDKSKLPNLKNAWPDITERLAKYDPGNEYAVNYMWGTTGIGYNVAKVKAALGDVPVDSWDVLF
KPENAEKLKSCGINILDASDETFAIAMNYLGKNPDSKETADLEAGGEVYSKIRPYVKTFNSSPYINDLANGDTCISIGWS
GDILQAKTRAEEAKNGVEVNYVIPKEGTYIWMDSFAIPADAKNVDEAHAFINYMMKPEVAAKASDYVQYANGNLPSQALM
DPSVAKNPGVYPDPETMKKLFTISPYGPKEQRVLNRVWTQIKTGT

Sequences:

>Translated_365_residues
MKNRSLRLTLALTTALVAAGSAMAQEKVVHVYNWSDYIDPVILEDFTKETGIKVVYDVYDGNEVLETKLLAGSSGYDVVA
PTSPFLARQIKAGVYQKLDKSKLPNLKNAWPDITERLAKYDPGNEYAVNYMWGTTGIGYNVAKVKAALGDVPVDSWDVLF
KPENAEKLKSCGINILDASDETFAIAMNYLGKNPDSKETADLEAGGEVYSKIRPYVKTFNSSPYINDLANGDTCISIGWS
GDILQAKTRAEEAKNGVEVNYVIPKEGTYIWMDSFAIPADAKNVDEAHAFINYMMKPEVAAKASDYVQYANGNLPSQALM
DPSVAKNPGVYPDPETMKKLFTISPYGPKEQRVLNRVWTQIKTGT
>Mature_365_residues
MKNRSLRLTLALTTALVAAGSAMAQEKVVHVYNWSDYIDPVILEDFTKETGIKVVYDVYDGNEVLETKLLAGSSGYDVVA
PTSPFLARQIKAGVYQKLDKSKLPNLKNAWPDITERLAKYDPGNEYAVNYMWGTTGIGYNVAKVKAALGDVPVDSWDVLF
KPENAEKLKSCGINILDASDETFAIAMNYLGKNPDSKETADLEAGGEVYSKIRPYVKTFNSSPYINDLANGDTCISIGWS
GDILQAKTRAEEAKNGVEVNYVIPKEGTYIWMDSFAIPADAKNVDEAHAFINYMMKPEVAAKASDYVQYANGNLPSQALM
DPSVAKNPGVYPDPETMKKLFTISPYGPKEQRVLNRVWTQIKTGT

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=350, Percent_Identity=55.4285714285714, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1787367, Length=312, Percent_Identity=36.8589743589744, Blast_Score=201, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40218; Mature: 40218

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRSLRLTLALTTALVAAGSAMAQEKVVHVYNWSDYIDPVILEDFTKETGIKVVYDVYD
CCCCCEEEEHHHHHHHHHHCHHHHHHCEEEEECCHHHCCHHHHHHHHHCCCCEEEEEECC
GNEVLETKLLAGSSGYDVVAPTSPFLARQIKAGVYQKLDKSKLPNLKNAWPDITERLAKY
CCHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
DPGNEYAVNYMWGTTGIGYNVAKVKAALGDVPVDSWDVLFKPENAEKLKSCGINILDASD
CCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCEEEECCC
ETFAIAMNYLGKNPDSKETADLEAGGEVYSKIRPYVKTFNSSPYINDLANGDTCISIGWS
CEEEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCC
GDILQAKTRAEEAKNGVEVNYVIPKEGTYIWMDSFAIPADAKNVDEAHAFINYMMKPEVA
CCHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECEECCCCCCCHHHHHHHHHHHHCCCHH
AKASDYVQYANGNLPSQALMDPSVAKNPGVYPDPETMKKLFTISPYGPKEQRVLNRVWTQ
HHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHEEECCCCCHHHHHHHHHHHH
IKTGT
HCCCC
>Mature Secondary Structure
MKNRSLRLTLALTTALVAAGSAMAQEKVVHVYNWSDYIDPVILEDFTKETGIKVVYDVYD
CCCCCEEEEHHHHHHHHHHCHHHHHHCEEEEECCHHHCCHHHHHHHHHCCCCEEEEEECC
GNEVLETKLLAGSSGYDVVAPTSPFLARQIKAGVYQKLDKSKLPNLKNAWPDITERLAKY
CCHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
DPGNEYAVNYMWGTTGIGYNVAKVKAALGDVPVDSWDVLFKPENAEKLKSCGINILDASD
CCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCEEEECCC
ETFAIAMNYLGKNPDSKETADLEAGGEVYSKIRPYVKTFNSSPYINDLANGDTCISIGWS
CEEEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCC
GDILQAKTRAEEAKNGVEVNYVIPKEGTYIWMDSFAIPADAKNVDEAHAFINYMMKPEVA
CCHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECEECCCCCCCHHHHHHHHHHHHCCCHH
AKASDYVQYANGNLPSQALMDPSVAKNPGVYPDPETMKKLFTISPYGPKEQRVLNRVWTQ
HHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHEEECCCCCHHHHHHHHHHHH
IKTGT
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]