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The map label for this gene is phoB [H]

Identifier: 15887773

GI number: 15887773

Start: 422089

End: 422772

Strand: Direct

Name: phoB [H]

Synonym: Atu0425

Alternate gene names: 15887773

Gene position: 422089-422772 (Clockwise)

Preceding gene: 15887772

Following gene: 159184303

Centisome position: 14.85

GC content: 61.4

Gene sequence:

>684_bases
ATGGTGCCGAAGATTGCAGTTGTGGAAGACGAGGAAGCGCTGAGCGTCCTGCTTCGTTACAATCTCGAGGCTGAGGGATA
CGACGTCGACACGATACCCCGTGGCGACGAGGCGGAAATCAGGTTGCAGGAGCGTATTCCGGATCTTCTCATCCTGGACT
GGATGCTGCCTGGCGTCTCCGGCATCGAACTTTGCCGGAGGCTCAGAATGCGGCCGGAAACGGAACGCCTGCCCATCATC
ATGCTGACGGCGCGTGGCGAAGAGAGCGAGCGCGTTCGTGGCCTTGCCACCGGCGCCGACGATTATGTCGTCAAGCCGTT
CTCGACGCCGGAACTCATGGCCCGCGTCAAGGCCATGCTGCGCCGGGCGCGTCCCGAGGTTCTGTCTTCGGTGCTGAAAT
GTGGTGATATCGAACTGGATCGCGAGACCCATCGCGTTCACCGCAAAAGCCGCGAAGTGCGCCTCGGCCCGACGGAATTC
CGCCTGCTGGAGTTCCTGATGACCTCGCCGGGCCGGGTGTTCTCCCGCTCGCAATTGCTGGATGGCGTCTGGGGCCACGA
TATCTACGTCGACGAGCGTACCGTTGACGTGCATGTCGGACGCCTGCGCAAGGCGCTCAATTTCTCCCATATGCAGGATG
TCATCCGCACCGTGCGTGGTGCCGGATATTCGATGGAAGCCTGA

Upstream 100 bases:

>100_bases
GCAGCCAGCCGGAGGGCGAGCGTCCGAAGGACGACAGTTCCAACACGCTCGGTTCGGTGACCGAGTAACAGACAGGCAGA
GGCTTGTGCGGAGTTAGCAT

Downstream 100 bases:

>100_bases
GAGGAACCGAACTGACGGTTCCTTTCAGCGCGACATACTCCAAAATCGAAAGGCCCGAAACCGGAATGGTGTCGGGCCTT
TTCATTTTGAACCTTCCGCA

Product: two component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MVPKIAVVEDEEALSVLLRYNLEAEGYDVDTIPRGDEAEIRLQERIPDLLILDWMLPGVSGIELCRRLRMRPETERLPII
MLTARGEESERVRGLATGADDYVVKPFSTPELMARVKAMLRRARPEVLSSVLKCGDIELDRETHRVHRKSREVRLGPTEF
RLLEFLMTSPGRVFSRSQLLDGVWGHDIYVDERTVDVHVGRLRKALNFSHMQDVIRTVRGAGYSMEA

Sequences:

>Translated_227_residues
MVPKIAVVEDEEALSVLLRYNLEAEGYDVDTIPRGDEAEIRLQERIPDLLILDWMLPGVSGIELCRRLRMRPETERLPII
MLTARGEESERVRGLATGADDYVVKPFSTPELMARVKAMLRRARPEVLSSVLKCGDIELDRETHRVHRKSREVRLGPTEF
RLLEFLMTSPGRVFSRSQLLDGVWGHDIYVDERTVDVHVGRLRKALNFSHMQDVIRTVRGAGYSMEA
>Mature_227_residues
MVPKIAVVEDEEALSVLLRYNLEAEGYDVDTIPRGDEAEIRLQERIPDLLILDWMLPGVSGIELCRRLRMRPETERLPII
MLTARGEESERVRGLATGADDYVVKPFSTPELMARVKAMLRRARPEVLSSVLKCGDIELDRETHRVHRKSREVRLGPTEF
RLLEFLMTSPGRVFSRSQLLDGVWGHDIYVDERTVDVHVGRLRKALNFSHMQDVIRTVRGAGYSMEA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=48.8789237668161, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=42.0353982300885, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1788394, Length=231, Percent_Identity=40.6926406926407, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=37.6681614349776, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI87082012, Length=225, Percent_Identity=37.3333333333333, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1786911, Length=229, Percent_Identity=37.5545851528384, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=36.4035087719298, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=35.4260089686099, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=36.036036036036, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=34.8214285714286, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1790863, Length=232, Percent_Identity=32.7586206896552, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=36.0869565217391, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787229, Length=234, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=31.3304721030043, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26008; Mature: 26008

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPKIAVVEDEEALSVLLRYNLEAEGYDVDTIPRGDEAEIRLQERIPDLLILDWMLPGVS
CCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEHHHHCCHHEEHHHHCCCCC
GIELCRRLRMRPETERLPIIMLTARGEESERVRGLATGADDYVVKPFSTPELMARVKAML
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
RRARPEVLSSVLKCGDIELDRETHRVHRKSREVRLGPTEFRLLEFLMTSPGRVFSRSQLL
HHCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
DGVWGHDIYVDERTVDVHVGRLRKALNFSHMQDVIRTVRGAGYSMEA
CCCCCCEEEECCCEEEHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVPKIAVVEDEEALSVLLRYNLEAEGYDVDTIPRGDEAEIRLQERIPDLLILDWMLPGVS
CCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEHHHHCCHHEEHHHHCCCCC
GIELCRRLRMRPETERLPIIMLTARGEESERVRGLATGADDYVVKPFSTPELMARVKAML
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
RRARPEVLSSVLKCGDIELDRETHRVHRKSREVRLGPTEFRLLEFLMTSPGRVFSRSQLL
HHCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
DGVWGHDIYVDERTVDVHVGRLRKALNFSHMQDVIRTVRGAGYSMEA
CCCCCCEEEECCCEEEHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]