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The map label for this gene is zraS [H]

Identifier: 15887737

GI number: 15887737

Start: 381014

End: 382489

Strand: Direct

Name: zraS [H]

Synonym: Atu0388

Alternate gene names: 15887737

Gene position: 381014-382489 (Clockwise)

Preceding gene: 159184286

Following gene: 15887738

Centisome position: 13.41

GC content: 61.72

Gene sequence:

>1476_bases
GTGTTTTCTAAAGGCGTAGACAGCTTGGACCGCACGCAAGACGATCCGGTGCATTGCATGCCGGGATTGACCGCCGGCCT
TTCCGGGCGGCTGCTGCTGCTGACCGTGATTTTCGTGATGATCGCCGAGGTGCTGATCTTCGTGCCATCGGTTGCCAATA
TGCGTCTTTCCTGGCTGCGTGACCGTCTGAACACGGCCGCCGCTGCCGGTGCTGTCATCGATGGCCTGCAGGCCGAACTG
CCGCGAAGCGTGCAGAACGACACGCTGATGGCAACCGGAGCGAAGCTAATCGCCCTGCGCAAGGATGGTACGTCGCGCCT
GCTGGCCGTAGCGGAAACGCCGCCCGGCGTCGACGAGCAATATGATCTTTCCGATGTTTCGCAGGCGGCGGCGGTGCGGG
ACGCCTTTTCCACCCTGCTGTTCGGCGGCAACCGCGTCATCCGCGTCTTCGATGTGGTGGGAGACAGCGACACGATCATC
GAGCTCGTACTGGGCGAAAACAAGCTGCGGGCGGCGATGCTTGCCTATGCTCGCAACGTTTTCCTGATTTCCATCGTCCT
GTCGCTGGTCACCGCGGGCCTGATCTTCATCTCCATCAACCGCATCATGATCCGGCCGATACGCCGGCTGACGCTCGGCA
TGCAGAATTTTTCGGAAGATCCGGGCAATCCCGAGCGGATTTTCGTGCCGGAGGAGGGAACCGACGAACTGTCGGTTGCG
GGCCGCCATCTCGCCGACATGCAGACGGAACTGCAGAAAACGCTGCGGCAGCAGAAGAACCTTGTGGATCTTGGCCTTGC
CGTGTCGAAGATCAACCATGACATGCGCAATATCCTCTCCTCCGCCCAGCTCATGTCAGACCGTCTGGTCGATGCCAAGG
ACCCGATGGTCAGGAGCTTCGCGCCGAAACTGCTGCGCACCATCGACCGTGCCGTGGCCTATACGGGTGAGGTTCTGGCC
TATGGTCAGGCGACGGAGGGGGCGCCAAAACGCAAGATAGTGCCATTGAGCCTGCTGGTGCGGGATGTGCGCGATATTCT
GGCCCTCGATCCCGATAGCGGCATCGAGTTTCACGACAGCGTCCCCCCCGATCTGACGGTGGATGCGGATTCCGAACAGC
TTTTCCGGGTGATCCACAATCTCTGTCGCAATGCCGTCCAGGCCCTGCAATCCGACGTGGCGAATTCGGCACCAAGGCAT
CTTTCCATCTCGGCCCAGAGGGCCGGCAGCGTCGTCAGCATCGTCATAGACGACAATGGGCCGGGAATGCCGCGCAAGGC
CAAGGAGAACCTGTTCTCCGCTTTCAGGGGATCGGCCCGTTCCGGCGGCACGGGCCTCGGGCTGGTCATTGCCCGTGAAC
TTGTGCTTGCCCACGGCGGTACGATCGCGCTTGTCGAAAAGCCCGGTCCCGGCACGCAGTTCCGTATCGAAATCGCCGAC
CGCGCCGCCTCCATACAGGCAGCCAGAACCGCCTGA

Upstream 100 bases:

>100_bases
CAGCGCATTGGAAAAATATCCATTAGGTCCTATTATCGTTGCTGCATATAAACCGAGCCGTTCCACGCAAGCAGCGGCGC
GCATACAGGATCATGGCCCG

Downstream 100 bases:

>100_bases
AAAACCCGCCCGGCTGCAGGTTCGGAAAGCCATTATCTCCGGCGGAATCAAGGCCCGATCAGGCCCGCGAAAAACAGCTT
GGATGGAATTTACAATATAA

Product: two component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MFSKGVDSLDRTQDDPVHCMPGLTAGLSGRLLLLTVIFVMIAEVLIFVPSVANMRLSWLRDRLNTAAAAGAVIDGLQAEL
PRSVQNDTLMATGAKLIALRKDGTSRLLAVAETPPGVDEQYDLSDVSQAAAVRDAFSTLLFGGNRVIRVFDVVGDSDTII
ELVLGENKLRAAMLAYARNVFLISIVLSLVTAGLIFISINRIMIRPIRRLTLGMQNFSEDPGNPERIFVPEEGTDELSVA
GRHLADMQTELQKTLRQQKNLVDLGLAVSKINHDMRNILSSAQLMSDRLVDAKDPMVRSFAPKLLRTIDRAVAYTGEVLA
YGQATEGAPKRKIVPLSLLVRDVRDILALDPDSGIEFHDSVPPDLTVDADSEQLFRVIHNLCRNAVQALQSDVANSAPRH
LSISAQRAGSVVSIVIDDNGPGMPRKAKENLFSAFRGSARSGGTGLGLVIARELVLAHGGTIALVEKPGPGTQFRIEIAD
RAASIQAARTA

Sequences:

>Translated_491_residues
MFSKGVDSLDRTQDDPVHCMPGLTAGLSGRLLLLTVIFVMIAEVLIFVPSVANMRLSWLRDRLNTAAAAGAVIDGLQAEL
PRSVQNDTLMATGAKLIALRKDGTSRLLAVAETPPGVDEQYDLSDVSQAAAVRDAFSTLLFGGNRVIRVFDVVGDSDTII
ELVLGENKLRAAMLAYARNVFLISIVLSLVTAGLIFISINRIMIRPIRRLTLGMQNFSEDPGNPERIFVPEEGTDELSVA
GRHLADMQTELQKTLRQQKNLVDLGLAVSKINHDMRNILSSAQLMSDRLVDAKDPMVRSFAPKLLRTIDRAVAYTGEVLA
YGQATEGAPKRKIVPLSLLVRDVRDILALDPDSGIEFHDSVPPDLTVDADSEQLFRVIHNLCRNAVQALQSDVANSAPRH
LSISAQRAGSVVSIVIDDNGPGMPRKAKENLFSAFRGSARSGGTGLGLVIARELVLAHGGTIALVEKPGPGTQFRIEIAD
RAASIQAARTA
>Mature_491_residues
MFSKGVDSLDRTQDDPVHCMPGLTAGLSGRLLLLTVIFVMIAEVLIFVPSVANMRLSWLRDRLNTAAAAGAVIDGLQAEL
PRSVQNDTLMATGAKLIALRKDGTSRLLAVAETPPGVDEQYDLSDVSQAAAVRDAFSTLLFGGNRVIRVFDVVGDSDTII
ELVLGENKLRAAMLAYARNVFLISIVLSLVTAGLIFISINRIMIRPIRRLTLGMQNFSEDPGNPERIFVPEEGTDELSVA
GRHLADMQTELQKTLRQQKNLVDLGLAVSKINHDMRNILSSAQLMSDRLVDAKDPMVRSFAPKLLRTIDRAVAYTGEVLA
YGQATEGAPKRKIVPLSLLVRDVRDILALDPDSGIEFHDSVPPDLTVDADSEQLFRVIHNLCRNAVQALQSDVANSAPRH
LSISAQRAGSVVSIVIDDNGPGMPRKAKENLFSAFRGSARSGGTGLGLVIARELVLAHGGTIALVEKPGPGTQFRIEIAD
RAASIQAARTA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=27.0042194092827, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790300, Length=227, Percent_Identity=30.3964757709251, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1786912, Length=278, Percent_Identity=26.6187050359712, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=27.2340425531915, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=194, Percent_Identity=28.3505154639175, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787894, Length=309, Percent_Identity=27.831715210356, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52960; Mature: 52960

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKGVDSLDRTQDDPVHCMPGLTAGLSGRLLLLTVIFVMIAEVLIFVPSVANMRLSWLR
CCCCCCCHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DRLNTAAAAGAVIDGLQAELPRSVQNDTLMATGAKLIALRKDGTSRLLAVAETPPGVDEQ
HHHHHHHHHHHHHHHHHHHCCCHHCCCEEEECCCEEEEEECCCCCEEEEEECCCCCCCCC
YDLSDVSQAAAVRDAFSTLLFGGNRVIRVFDVVGDSDTIIELVLGENKLRAAMLAYARNV
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHH
FLISIVLSLVTAGLIFISINRIMIRPIRRLTLGMQNFSEDPGNPERIFVPEEGTDELSVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHH
GRHLADMQTELQKTLRQQKNLVDLGLAVSKINHDMRNILSSAQLMSDRLVDAKDPMVRSF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
APKLLRTIDRAVAYTGEVLAYGQATEGAPKRKIVPLSLLVRDVRDILALDPDSGIEFHDS
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
VPPDLTVDADSEQLFRVIHNLCRNAVQALQSDVANSAPRHLSISAQRAGSVVSIVIDDNG
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCEEEEEEECCC
PGMPRKAKENLFSAFRGSARSGGTGLGLVIARELVLAHGGTIALVEKPGPGTQFRIEIAD
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECC
RAASIQAARTA
CHHHHHHHCCC
>Mature Secondary Structure
MFSKGVDSLDRTQDDPVHCMPGLTAGLSGRLLLLTVIFVMIAEVLIFVPSVANMRLSWLR
CCCCCCCHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DRLNTAAAAGAVIDGLQAELPRSVQNDTLMATGAKLIALRKDGTSRLLAVAETPPGVDEQ
HHHHHHHHHHHHHHHHHHHCCCHHCCCEEEECCCEEEEEECCCCCEEEEEECCCCCCCCC
YDLSDVSQAAAVRDAFSTLLFGGNRVIRVFDVVGDSDTIIELVLGENKLRAAMLAYARNV
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHH
FLISIVLSLVTAGLIFISINRIMIRPIRRLTLGMQNFSEDPGNPERIFVPEEGTDELSVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHH
GRHLADMQTELQKTLRQQKNLVDLGLAVSKINHDMRNILSSAQLMSDRLVDAKDPMVRSF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
APKLLRTIDRAVAYTGEVLAYGQATEGAPKRKIVPLSLLVRDVRDILALDPDSGIEFHDS
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
VPPDLTVDADSEQLFRVIHNLCRNAVQALQSDVANSAPRHLSISAQRAGSVVSIVIDDNG
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCEEEEEEECCC
PGMPRKAKENLFSAFRGSARSGGTGLGLVIARELVLAHGGTIALVEKPGPGTQFRIEIAD
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECC
RAASIQAARTA
CHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]