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The map label for this gene is cysJ [H]

Identifier: 15887500

GI number: 15887500

Start: 154282

End: 156051

Strand: Direct

Name: cysJ [H]

Synonym: Atu0146

Alternate gene names: 15887500

Gene position: 154282-156051 (Clockwise)

Preceding gene: 159184180

Following gene: 159184186

Centisome position: 5.43

GC content: 60.85

Gene sequence:

>1770_bases
ATGATGAAGATACCTTACATTCCACAGGATGCGCCCTTCAACGGAGATCAGCGCGCCTGGCTCGCCGGCTTTCTGGCCGG
GCTCCACTCCCGCACCGCCATCGGTCTGGAGACCTCCGTCGCCGCCGCTGCCCCCAAATCGGCGGCCAGCGTGTTGAACA
TCCTTGTCGGCACCCAGACGGGCAATGCCGAAGCGCTTGCCATGGATATCGCCGCTGCGGCGCGCACTCAGGGCATGCAG
CCGGTGGTGACGGCTCTGGATGACGTCTCCATGGACAGCCTCGGCTCCATGAAGCGGGTCATCGTCGTCACTTCGACCTA
TGGCGAAGGCGAGATGCCTGACAATGCCCAGCTGTTCTGGGAGGCGCTTGCTGCTGACAGCGCGCCGCGGCTGGACGAGA
TGAATTTCGCCGTACTTGCCTTGGGTGATACCGGTTATGACGGGTTCTGCCAGGCGGGTAAGCTTATCGACACGCGTTTT
GAACAGCTCGGCGGCAGGCGCATGAAAGCGCGTATCGACTGCGATATCGACTTTGAGGATGCAGCCGCTGCGTGGATCGG
TGAGGCCCTGCCGCTTGCGCTGGCCGTCGATGGCGGCAAGGAAGCCGCTGTCACCATGCCCGACATCGCCGCGCAGCCAA
CGCGGGCAAAATCGGGCTGGAGCCGCAAGAACCCCTATCCAGCGACGGTAGGCGTTAATCGCCGCCTGTCCGGTTCCGGC
TCGTCCAAGGAAATCCGCCACTACGAATTCGATCTCCGCGAAAGCGGGCTCGATTATGAGGCGGGTGACGCGCTTGGTGT
TGTTCCGCTTAACAATCCGAAACTGGCCGGCGCGCTTCTCGGCCATTTCGGAGCGGTCGGAGACAGCCAAGTCGCCGGGC
TGGACCGGCCGGTTGGCGATGCGCTTCTCAACATCTACGAGATTTCAACGCCCTCACGCGAACTGGTGGCTGAAATCGGC
AAGCGCGCTGGGCATGAAGAACTTTCCCATATCCTTGCGAATGGCGACAAGGAGCAGCTGGCCGCCTTTCTCTGGAGCAA
GGATATTCTCGACCTGCTCAATCTAGGCGGAAAGCCGCTCTTCGAGCTTGCCGAATTCGTCACCTTCCTGAAACCCTTGC
AGCACCGCGCCTATTCCATCTCCTCAAGCCCTCTGCTTGCGAAAGACAGCGTGCATCTGACGATCGCCAGCGTGCGGTAT
CGCGCCGAGGGCCGGGAGCGTGGCGGCGTATGCTCCACCTATCTCGCAGATCGGGTGGAGGCTGGGCAGAGCGCCGGTAT
TTTCGTTTCGCCCAACAAGGCATTCCGTGTCCCGCAGGACGATAATGCGCCTGTTATCATGGTTGGACCGGGCACGGGTA
TTGCGCCGTTTCGGGCCTTTTTGCAGGAACGGCAGGCACGCGGCGCCAGGGGCAAGAACTGGCTGTTTTTCGGCGACCAG
CACCGGCAATCGGACTTCATCTATGAAAACGAACTTGGCGACATGAGCCGCGACGGCGTGCTGACACGCCTCGACCTTGC
TTTTTCACGGGATCAGGCGGAGAAAATCTATGTCCAGACCCGCATGCGGCAGAACGGTAAGGCGCTTTATCAATGGCTGG
AGGAGGGCGGTTATTTCTACGTCTGCGGCGATGCGACCCGTATGGCCAAGGATGTCGATGACGCCCTGCACAGCATCATA
AGCGATGAGGCCGGCATCTCCGCCGAGGCCGCAAGCGACTATGTCAACCGGCTAAAGCGTGAAAAACGCTATCTGCGCGA
CGTCTATTAG

Upstream 100 bases:

>100_bases
AAAACATCGAGAAGATCGGCCGTCTGCCGGGCTTCGAGGAGCTTTTCGCCGCGCGCGCCATTCCGGACCTTGCCACCGCA
TCCAGTCATAGCAGGGCGGC

Downstream 100 bases:

>100_bases
ACCCAACCGTAAAAGGGGCCGCCGGAGCACCGATTGCCTCAGCAATTGGTGCAGGCGGTCTCGGCGTCACGCTGCTTGAA
GAAATAATCCCTGCCGATCG

Product: sulfite reductase (NADPH) flavoprotein alpha-component

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MMKIPYIPQDAPFNGDQRAWLAGFLAGLHSRTAIGLETSVAAAAPKSAASVLNILVGTQTGNAEALAMDIAAAARTQGMQ
PVVTALDDVSMDSLGSMKRVIVVTSTYGEGEMPDNAQLFWEALAADSAPRLDEMNFAVLALGDTGYDGFCQAGKLIDTRF
EQLGGRRMKARIDCDIDFEDAAAAWIGEALPLALAVDGGKEAAVTMPDIAAQPTRAKSGWSRKNPYPATVGVNRRLSGSG
SSKEIRHYEFDLRESGLDYEAGDALGVVPLNNPKLAGALLGHFGAVGDSQVAGLDRPVGDALLNIYEISTPSRELVAEIG
KRAGHEELSHILANGDKEQLAAFLWSKDILDLLNLGGKPLFELAEFVTFLKPLQHRAYSISSSPLLAKDSVHLTIASVRY
RAEGRERGGVCSTYLADRVEAGQSAGIFVSPNKAFRVPQDDNAPVIMVGPGTGIAPFRAFLQERQARGARGKNWLFFGDQ
HRQSDFIYENELGDMSRDGVLTRLDLAFSRDQAEKIYVQTRMRQNGKALYQWLEEGGYFYVCGDATRMAKDVDDALHSII
SDEAGISAEAASDYVNRLKREKRYLRDVY

Sequences:

>Translated_589_residues
MMKIPYIPQDAPFNGDQRAWLAGFLAGLHSRTAIGLETSVAAAAPKSAASVLNILVGTQTGNAEALAMDIAAAARTQGMQ
PVVTALDDVSMDSLGSMKRVIVVTSTYGEGEMPDNAQLFWEALAADSAPRLDEMNFAVLALGDTGYDGFCQAGKLIDTRF
EQLGGRRMKARIDCDIDFEDAAAAWIGEALPLALAVDGGKEAAVTMPDIAAQPTRAKSGWSRKNPYPATVGVNRRLSGSG
SSKEIRHYEFDLRESGLDYEAGDALGVVPLNNPKLAGALLGHFGAVGDSQVAGLDRPVGDALLNIYEISTPSRELVAEIG
KRAGHEELSHILANGDKEQLAAFLWSKDILDLLNLGGKPLFELAEFVTFLKPLQHRAYSISSSPLLAKDSVHLTIASVRY
RAEGRERGGVCSTYLADRVEAGQSAGIFVSPNKAFRVPQDDNAPVIMVGPGTGIAPFRAFLQERQARGARGKNWLFFGDQ
HRQSDFIYENELGDMSRDGVLTRLDLAFSRDQAEKIYVQTRMRQNGKALYQWLEEGGYFYVCGDATRMAKDVDDALHSII
SDEAGISAEAASDYVNRLKREKRYLRDVY
>Mature_589_residues
MMKIPYIPQDAPFNGDQRAWLAGFLAGLHSRTAIGLETSVAAAAPKSAASVLNILVGTQTGNAEALAMDIAAAARTQGMQ
PVVTALDDVSMDSLGSMKRVIVVTSTYGEGEMPDNAQLFWEALAADSAPRLDEMNFAVLALGDTGYDGFCQAGKLIDTRF
EQLGGRRMKARIDCDIDFEDAAAAWIGEALPLALAVDGGKEAAVTMPDIAAQPTRAKSGWSRKNPYPATVGVNRRLSGSG
SSKEIRHYEFDLRESGLDYEAGDALGVVPLNNPKLAGALLGHFGAVGDSQVAGLDRPVGDALLNIYEISTPSRELVAEIG
KRAGHEELSHILANGDKEQLAAFLWSKDILDLLNLGGKPLFELAEFVTFLKPLQHRAYSISSSPLLAKDSVHLTIASVRY
RAEGRERGGVCSTYLADRVEAGQSAGIFVSPNKAFRVPQDDNAPVIMVGPGTGIAPFRAFLQERQARGARGKNWLFFGDQ
HRQSDFIYENELGDMSRDGVLTRLDLAFSRDQAEKIYVQTRMRQNGKALYQWLEEGGYFYVCGDATRMAKDVDDALHSII
SDEAGISAEAASDYVNRLKREKRYLRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=626, Percent_Identity=30.3514376996805, Blast_Score=254, Evalue=2e-67,
Organism=Homo sapiens, GI7657393, Length=607, Percent_Identity=30.1482701812191, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI221316709, Length=607, Percent_Identity=29.8187808896211, Blast_Score=220, Evalue=3e-57,
Organism=Homo sapiens, GI221316705, Length=616, Percent_Identity=29.7077922077922, Blast_Score=219, Evalue=6e-57,
Organism=Homo sapiens, GI24041029, Length=598, Percent_Identity=28.9297658862876, Blast_Score=209, Evalue=9e-54,
Organism=Homo sapiens, GI221316707, Length=537, Percent_Identity=29.7951582867784, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI10835173, Length=652, Percent_Identity=28.2208588957055, Blast_Score=180, Evalue=4e-45,
Organism=Homo sapiens, GI40254422, Length=647, Percent_Identity=27.8207109737249, Blast_Score=180, Evalue=4e-45,
Organism=Homo sapiens, GI169790956, Length=260, Percent_Identity=37.6923076923077, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI169790958, Length=260, Percent_Identity=37.6923076923077, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1789123, Length=584, Percent_Identity=39.041095890411, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17554134, Length=619, Percent_Identity=31.0177705977383, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17566446, Length=588, Percent_Identity=27.0408163265306, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17531441, Length=406, Percent_Identity=24.6305418719212, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6321143, Length=394, Percent_Identity=30.2030456852792, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321832, Length=637, Percent_Identity=27.7864992150706, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6325305, Length=640, Percent_Identity=23.4375, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322597, Length=175, Percent_Identity=32.5714285714286, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17137192, Length=612, Percent_Identity=30.0653594771242, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24582192, Length=520, Percent_Identity=30.3846153846154, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24583543, Length=663, Percent_Identity=27.7526395173454, Blast_Score=195, Evalue=6e-50,
Organism=Drosophila melanogaster, GI78706876, Length=663, Percent_Identity=27.7526395173454, Blast_Score=195, Evalue=6e-50,
Organism=Drosophila melanogaster, GI78706872, Length=663, Percent_Identity=27.7526395173454, Blast_Score=195, Evalue=8e-50,
Organism=Drosophila melanogaster, GI24660907, Length=600, Percent_Identity=26.6666666666667, Blast_Score=175, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24660903, Length=600, Percent_Identity=26.6666666666667, Blast_Score=175, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24647438, Length=414, Percent_Identity=22.2222222222222, Blast_Score=81, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 63878; Mature: 63878

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00018 EF_HAND_1 ; PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIPYIPQDAPFNGDQRAWLAGFLAGLHSRTAIGLETSVAAAAPKSAASVLNILVGTQT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCHHHHHCCCHHHHHHHHHHEECCC
GNAEALAMDIAAAARTQGMQPVVTALDDVSMDSLGSMKRVIVVTSTYGEGEMPDNAQLFW
CCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCCEEEEEEEECCCCCCCCCCHHHHH
EALAADSAPRLDEMNFAVLALGDTGYDGFCQAGKLIDTRFEQLGGRRMKARIDCDIDFED
HHHHCCCCCCCCCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHCCCEEEEEEECCCCHHH
AAAAWIGEALPLALAVDGGKEAAVTMPDIAAQPTRAKSGWSRKNPYPATVGVNRRLSGSG
HHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCEECCCC
SSKEIRHYEFDLRESGLDYEAGDALGVVPLNNPKLAGALLGHFGAVGDSQVAGLDRPVGD
CCCCCEEEEECHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHCCCCCCHHH
ALLNIYEISTPSRELVAEIGKRAGHEELSHILANGDKEQLAAFLWSKDILDLLNLGGKPL
HHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCH
FELAEFVTFLKPLQHRAYSISSSPLLAKDSVHLTIASVRYRAEGRERGGVCSTYLADRVE
HHHHHHHHHHHHHHHHHEECCCCCCEECCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHH
AGQSAGIFVSPNKAFRVPQDDNAPVIMVGPGTGIAPFRAFLQERQARGARGKNWLFFGDQ
CCCCCCEEECCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCC
HRQSDFIYENELGDMSRDGVLTRLDLAFSRDQAEKIYVQTRMRQNGKALYQWLEEGGYFY
CCCCCCEEECCCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHCCEEE
VCGDATRMAKDVDDALHSIISDEAGISAEAASDYVNRLKREKRYLRDVY
EECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMKIPYIPQDAPFNGDQRAWLAGFLAGLHSRTAIGLETSVAAAAPKSAASVLNILVGTQT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCHHHHHCCCHHHHHHHHHHEECCC
GNAEALAMDIAAAARTQGMQPVVTALDDVSMDSLGSMKRVIVVTSTYGEGEMPDNAQLFW
CCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCCEEEEEEEECCCCCCCCCCHHHHH
EALAADSAPRLDEMNFAVLALGDTGYDGFCQAGKLIDTRFEQLGGRRMKARIDCDIDFED
HHHHCCCCCCCCCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHCCCEEEEEEECCCCHHH
AAAAWIGEALPLALAVDGGKEAAVTMPDIAAQPTRAKSGWSRKNPYPATVGVNRRLSGSG
HHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCEECCCC
SSKEIRHYEFDLRESGLDYEAGDALGVVPLNNPKLAGALLGHFGAVGDSQVAGLDRPVGD
CCCCCEEEEECHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHCCCCCCHHH
ALLNIYEISTPSRELVAEIGKRAGHEELSHILANGDKEQLAAFLWSKDILDLLNLGGKPL
HHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCH
FELAEFVTFLKPLQHRAYSISSSPLLAKDSVHLTIASVRYRAEGRERGGVCSTYLADRVE
HHHHHHHHHHHHHHHHHEECCCCCCEECCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHH
AGQSAGIFVSPNKAFRVPQDDNAPVIMVGPGTGIAPFRAFLQERQARGARGKNWLFFGDQ
CCCCCCEEECCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCC
HRQSDFIYENELGDMSRDGVLTRLDLAFSRDQAEKIYVQTRMRQNGKALYQWLEEGGYFY
CCCCCCEEECCCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHCCEEE
VCGDATRMAKDVDDALHSIISDEAGISAEAASDYVNRLKREKRYLRDVY
EECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]