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The map label for this gene is mdeA [H]

Identifier: 158423804

GI number: 158423804

Start: 2496783

End: 2498066

Strand: Direct

Name: mdeA [H]

Synonym: AZC_2180

Alternate gene names: 158423804

Gene position: 2496783-2498066 (Clockwise)

Preceding gene: 158423803

Following gene: 158423806

Centisome position: 46.5

GC content: 65.89

Gene sequence:

>1284_bases
ATGAGCGAACGCACGCCCGGTTTTTCCACCCTCGCCATTCACGCGGGGGCTCAGCCCGACCCCACCACCGGCGCGCGGGC
AACGCCCATCTACCAGACCACCTCCTTCGTCTTCGACGACGTGGACCATGCGGCGGCGCTGTTCGGGCTCCAGACCTTCG
GCAACATCTACACGCGCATCGGCAACCCGACCGTCGCTGTGCTGGAGGAGCGGGTGGCGGCGCTGGAAGGCGGCACGGCG
GCGCTTGCGGTCGCATCCGGCCATGCGGCGCAGCATCTGGTGTTCCACACGCTGCTGACGTCTGGCGACGAATTCGTCGC
CTCGCGCAAGCTCTATGGCGGCTCCATCAACCAGTTCAACCATGCCTTCAAGAGCTACGGCTGGAACGTCGCCTGGGCCG
ATCCCGATAATGTCTCCAGCTTCGAGCGGGCCATCACGCCGAAGACCAAGGCGATCTTCATCGAGAGCCTCGCCAACCCC
GGCGGCATCGTCACCGACATCAAGGCCATCTCGGCGGTCGCGAAGAAGGCAGGCATCCCGCTGATCGTCGATAACACGCT
GGCGACGCCCTACCTCTGGCGGCCAATCGAGCATGGCGCGGACATCGTCATCCATTCCGCCACCAAGTTCCTGGGCGGTC
ACGGCAATTCCATCGGCGGCGTGATCGTGGACGCGGGCACGTTCGACTGGACGGCCAGCGGGCGCTATCCTTTCCTCACC
CAGCCCCGGCCCGAGTATCAGGGCATCGTGCTGCACGAGACCTTCGGTAATTTCGCCTTCGCCATCGCCGCCCGCGTCCT
GGGCCTGCGCGATCTCGGGCCGGCGCTCTCGCCCTTCAATGCCTTCCTGATCCTCACCGGCATCGAGACGCTGGGACTGC
GCGTGCAGCGCCACAGCGACAATGCGCTGGCGGTGGCCAATTTCCTGAGCACCCATCCGGCAGTGGAAGGGGTGAGCTAT
GCGGGGCTGCCGGGTGACCGCTACCATGACCTCGCCAAGGCCTATCTGCCGAAGGGCGCGGGTGCGGTCTTCACCTTCCG
GCTGAAGGGCGGCTATCAGGCGGGCGTGAACCTCGTCTCCAATGTCGAGCTGTTCTCGCATCTCGCCAATATCGGCGACA
CCCGCTCGCTCATCATCCACCCCGCTTCCACCACCCACCGCCAGCTCTCGGACGAGCAGAAGACGCTGGCCGGTGCCGGC
CCCGAGGTGGTGCGTCTGTCGGTCGGCATCGAGGACGTGGCGGACATCATTGCCGATCTCGATCAGGCGCTTGCGAAGGC
CTGA

Upstream 100 bases:

>100_bases
CTGCATGATCCATAATTGCACGATCAAACCATGTTCGTCGTGCACATAAGCGCATCCGCCGGGCGGCTTCCGGCGCGGTG
CCATACGGAGAGGCAAGCCC

Downstream 100 bases:

>100_bases
GGCGGCTCGCGCCCGCGCTGGCCGGATGGCCGGCGCGGGTATCCATCAGTTGCGCGCGCCGTGGACCTCGCGGGCGAGCG
CGAGCCAGGCGCGGGCGGCG

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSERTPGFSTLAIHAGAQPDPTTGARATPIYQTTSFVFDDVDHAAALFGLQTFGNIYTRIGNPTVAVLEERVAALEGGTA
ALAVASGHAAQHLVFHTLLTSGDEFVASRKLYGGSINQFNHAFKSYGWNVAWADPDNVSSFERAITPKTKAIFIESLANP
GGIVTDIKAISAVAKKAGIPLIVDNTLATPYLWRPIEHGADIVIHSATKFLGGHGNSIGGVIVDAGTFDWTASGRYPFLT
QPRPEYQGIVLHETFGNFAFAIAARVLGLRDLGPALSPFNAFLILTGIETLGLRVQRHSDNALAVANFLSTHPAVEGVSY
AGLPGDRYHDLAKAYLPKGAGAVFTFRLKGGYQAGVNLVSNVELFSHLANIGDTRSLIIHPASTTHRQLSDEQKTLAGAG
PEVVRLSVGIEDVADIIADLDQALAKA

Sequences:

>Translated_427_residues
MSERTPGFSTLAIHAGAQPDPTTGARATPIYQTTSFVFDDVDHAAALFGLQTFGNIYTRIGNPTVAVLEERVAALEGGTA
ALAVASGHAAQHLVFHTLLTSGDEFVASRKLYGGSINQFNHAFKSYGWNVAWADPDNVSSFERAITPKTKAIFIESLANP
GGIVTDIKAISAVAKKAGIPLIVDNTLATPYLWRPIEHGADIVIHSATKFLGGHGNSIGGVIVDAGTFDWTASGRYPFLT
QPRPEYQGIVLHETFGNFAFAIAARVLGLRDLGPALSPFNAFLILTGIETLGLRVQRHSDNALAVANFLSTHPAVEGVSY
AGLPGDRYHDLAKAYLPKGAGAVFTFRLKGGYQAGVNLVSNVELFSHLANIGDTRSLIIHPASTTHRQLSDEQKTLAGAG
PEVVRLSVGIEDVADIIADLDQALAKA
>Mature_426_residues
SERTPGFSTLAIHAGAQPDPTTGARATPIYQTTSFVFDDVDHAAALFGLQTFGNIYTRIGNPTVAVLEERVAALEGGTAA
LAVASGHAAQHLVFHTLLTSGDEFVASRKLYGGSINQFNHAFKSYGWNVAWADPDNVSSFERAITPKTKAIFIESLANPG
GIVTDIKAISAVAKKAGIPLIVDNTLATPYLWRPIEHGADIVIHSATKFLGGHGNSIGGVIVDAGTFDWTASGRYPFLTQ
PRPEYQGIVLHETFGNFAFAIAARVLGLRDLGPALSPFNAFLILTGIETLGLRVQRHSDNALAVANFLSTHPAVEGVSYA
GLPGDRYHDLAKAYLPKGAGAVFTFRLKGGYQAGVNLVSNVELFSHLANIGDTRSLIIHPASTTHRQLSDEQKTLAGAGP
EVVRLSVGIEDVADIIADLDQALAKA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=34.7517730496454, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=31.4420803782506, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=30.8056872037915, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1790375, Length=424, Percent_Identity=30.6603773584906, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1789383, Length=436, Percent_Identity=26.1467889908257, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71980708, Length=418, Percent_Identity=34.2105263157895, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17538055, Length=427, Percent_Identity=33.9578454332553, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17560056, Length=426, Percent_Identity=34.7417840375587, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=46.2962962962963, Blast_Score=340, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6319307, Length=432, Percent_Identity=31.25, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6321254, Length=446, Percent_Identity=26.457399103139, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=365, Percent_Identity=27.3972602739726, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=432, Percent_Identity=25, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922554, Length=428, Percent_Identity=32.4766355140187, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45246; Mature: 45114

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERTPGFSTLAIHAGAQPDPTTGARATPIYQTTSFVFDDVDHAAALFGLQTFGNIYTRI
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHCHHHHHHHHHHHHHHHHHHHC
GNPTVAVLEERVAALEGGTAALAVASGHAAQHLVFHTLLTSGDEFVASRKLYGGSINQFN
CCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHH
HAFKSYGWNVAWADPDNVSSFERAITPKTKAIFIESLANPGGIVTDIKAISAVAKKAGIP
HHHHHCCCEEEECCCCCHHHHHHHCCCCHHEEEEEHHCCCCCHHHHHHHHHHHHHHCCCC
LIVDNTLATPYLWRPIEHGADIVIHSATKFLGGHGNSIGGVIVDAGTFDWTASGRYPFLT
EEEECCCCCCCEECCCCCCCCEEEEEHHHHHCCCCCCCCEEEEECCCCEEECCCCCCCCC
QPRPEYQGIVLHETFGNFAFAIAARVLGLRDLGPALSPFNAFLILTGIETLGLRVQRHSD
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHCEEEEECCC
NALAVANFLSTHPAVEGVSYAGLPGDRYHDLAKAYLPKGAGAVFTFRLKGGYQAGVNLVS
CHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHCCHHHH
NVELFSHLANIGDTRSLIIHPASTTHRQLSDEQKTLAGAGPEVVRLSVGIEDVADIIADL
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHH
DQALAKA
HHHHHCC
>Mature Secondary Structure 
SERTPGFSTLAIHAGAQPDPTTGARATPIYQTTSFVFDDVDHAAALFGLQTFGNIYTRI
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHCHHHHHHHHHHHHHHHHHHHC
GNPTVAVLEERVAALEGGTAALAVASGHAAQHLVFHTLLTSGDEFVASRKLYGGSINQFN
CCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHH
HAFKSYGWNVAWADPDNVSSFERAITPKTKAIFIESLANPGGIVTDIKAISAVAKKAGIP
HHHHHCCCEEEECCCCCHHHHHHHCCCCHHEEEEEHHCCCCCHHHHHHHHHHHHHHCCCC
LIVDNTLATPYLWRPIEHGADIVIHSATKFLGGHGNSIGGVIVDAGTFDWTASGRYPFLT
EEEECCCCCCCEECCCCCCCCEEEEEHHHHHCCCCCCCCEEEEECCCCEEECCCCCCCCC
QPRPEYQGIVLHETFGNFAFAIAARVLGLRDLGPALSPFNAFLILTGIETLGLRVQRHSD
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHCEEEEECCC
NALAVANFLSTHPAVEGVSYAGLPGDRYHDLAKAYLPKGAGAVFTFRLKGGYQAGVNLVS
CHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHCCHHHH
NVELFSHLANIGDTRSLIIHPASTTHRQLSDEQKTLAGAGPEVVRLSVGIEDVADIIADL
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHH
DQALAKA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]