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The map label for this gene is kgtP [H]

Identifier: 158423711

GI number: 158423711

Start: 2399711

End: 2401276

Strand: Direct

Name: kgtP [H]

Synonym: AZC_2087

Alternate gene names: 158423711

Gene position: 2399711-2401276 (Clockwise)

Preceding gene: 158423710

Following gene: 158423715

Centisome position: 44.69

GC content: 64.24

Gene sequence:

>1566_bases
TTGCGTCGCCTCACAGTTATTCCCTGCTGCCGGTCGGTATTCCGGGTGCGTGTTTTCACCGCGTCGCTGTTGGGCGGCAG
GTGCGGCAGCAGCGCCCCAACGGGGCCGCAGACGCCTCGGGATCAGCGGGAGCGAAGGGGGCCGGACGGGCGTCACGCCC
GTGCGGCCGCGTGGCTTGAAGGCATGCGAACCCAGGATCTCCCGGACGATATCTGGCGCGAGGGAGACGATATGAAACGT
CGTACCGATGAAGACTTCCGCCCCGTCGGGGATGGGTATGACACCAAGGACACGGTGCGCCGTGTCAAGGCGATCCTGGT
CGGATCGACCGGCAACCTGATCGAGTGGTACGACGTCTACGTCTTCGCCGCTTTCCAGCTTTACTTTGCTCCGTCCTTCT
TTGCTGACCAGTCTCCGGCCCGTCAGCAGATCTTTGCTTCCATCGTCTTCGCGCTCGGCTTCCTTGCGCGCCCGTTCGGC
AGCCTCCTGTTCGGCGCCATCGCGGACCGCTACGGCCGCCGGATCTCGCTCACCGCGTCCGTGCTGCTGATGTGCTTCGG
CTCGCTCATCATCGCCTGCACCCCCTCTGCGACCCAGATCGGCATCGCCGCGCCCATCCTGCTCACGGTCGCCCGCCTCC
TGCAGGGCCTGAGCCAGGGCGGCGAATACGGCGCCAGCGCCACCTATCTCTCGGAGATGTCGCACCCCAACCGCCGCGGC
TTCTATTCCGGCGTCTGGTACGTGACGCTGATCGGCGGCCAGCTGATGGCGGTGTTCACCCTGCTCATCATGCAGAAGCT
GTTCCTCTCGGAGGACCAGATCCGGGCCTGGGGCTGGCGCATTCCCTTCTTCATCGGCGCGGCGCTGGCGGTTTATTCCT
TCACCATGCGGCGGGACATGCCGGAGACCGACCTGTTCAATAAGGCGAAGTCGGTCGCCAAGGAGCGCGCCCCCCTCGCC
GATCTCATGCGGCACTGGAAGTCGCTGCTGCTCGTGGTGGGCGTGACCATCGGCGGCACCTCCGCCTTCTACACCTACAC
CACCTACATGCAGAAGTTCCTGAAGCAGTCGGTGGGCCTCACGGCGGACCAGACCACCATGGTCACGGTCGGCACGCTCA
TCGTCGCTTTGATCATGCAGCCCCTCTACGGCTCGATCTCCGACAAGATCGGCCGCAAGCCGCTTCTGATCTACTTCGGC
GTGATGGGTACGCTGTTCACCTATCCGCTGCTGAACACCATCCAGACCACCAAGGACCCTTGGATGGCCTTCCTGCTGAT
CTGCGGCGGCTGGGCCATCGTCAGCGGCTATACGTCCATCACGGCCATCATCAAGGCGGAGCTGTTCCCCACCTCCGTGC
GTGCCATGGGCGTGGGCTTCCCCTATGCCGTCACGGTCGCCGTGTTCGGCGGCACCGTGGACAGCGTGGCCCAGATCTTC
AAGTTCGAGCTCCATTGGGAGCAGGGCTTCTACTGGTACGCCACCGCCTGCATCTTCGTGTCGCTGCTGGTCTATATCGG
CCTCAAGGACACCAAGAAGACCTCGCGGATGGACGAGGCCCACTGA

Upstream 100 bases:

>100_bases
GGGGGTTGCGCTCGCGCGAAAAAAGGTGAATTAAGCTGACCTAATCGCCGCAGTATTCTGTCAGCTAACGCAACGTTAGG
CAGCGGATCACTCGCTCACG

Downstream 100 bases:

>100_bases
TCGGGCGCTCTTCCGCATTCACGTGAAAGAAAGCCGCGCGGCGCTCATCGCTCCGCGCGGCTTTTTCATGTCGGGACGAG
CGTCAGGCGCCGAGCAGGGC

Product: MFS dicarboxylate transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MRRLTVIPCCRSVFRVRVFTASLLGGRCGSSAPTGPQTPRDQRERRGPDGRHARAAAWLEGMRTQDLPDDIWREGDDMKR
RTDEDFRPVGDGYDTKDTVRRVKAILVGSTGNLIEWYDVYVFAAFQLYFAPSFFADQSPARQQIFASIVFALGFLARPFG
SLLFGAIADRYGRRISLTASVLLMCFGSLIIACTPSATQIGIAAPILLTVARLLQGLSQGGEYGASATYLSEMSHPNRRG
FYSGVWYVTLIGGQLMAVFTLLIMQKLFLSEDQIRAWGWRIPFFIGAALAVYSFTMRRDMPETDLFNKAKSVAKERAPLA
DLMRHWKSLLLVVGVTIGGTSAFYTYTTYMQKFLKQSVGLTADQTTMVTVGTLIVALIMQPLYGSISDKIGRKPLLIYFG
VMGTLFTYPLLNTIQTTKDPWMAFLLICGGWAIVSGYTSITAIIKAELFPTSVRAMGVGFPYAVTVAVFGGTVDSVAQIF
KFELHWEQGFYWYATACIFVSLLVYIGLKDTKKTSRMDEAH

Sequences:

>Translated_521_residues
MRRLTVIPCCRSVFRVRVFTASLLGGRCGSSAPTGPQTPRDQRERRGPDGRHARAAAWLEGMRTQDLPDDIWREGDDMKR
RTDEDFRPVGDGYDTKDTVRRVKAILVGSTGNLIEWYDVYVFAAFQLYFAPSFFADQSPARQQIFASIVFALGFLARPFG
SLLFGAIADRYGRRISLTASVLLMCFGSLIIACTPSATQIGIAAPILLTVARLLQGLSQGGEYGASATYLSEMSHPNRRG
FYSGVWYVTLIGGQLMAVFTLLIMQKLFLSEDQIRAWGWRIPFFIGAALAVYSFTMRRDMPETDLFNKAKSVAKERAPLA
DLMRHWKSLLLVVGVTIGGTSAFYTYTTYMQKFLKQSVGLTADQTTMVTVGTLIVALIMQPLYGSISDKIGRKPLLIYFG
VMGTLFTYPLLNTIQTTKDPWMAFLLICGGWAIVSGYTSITAIIKAELFPTSVRAMGVGFPYAVTVAVFGGTVDSVAQIF
KFELHWEQGFYWYATACIFVSLLVYIGLKDTKKTSRMDEAH
>Mature_521_residues
MRRLTVIPCCRSVFRVRVFTASLLGGRCGSSAPTGPQTPRDQRERRGPDGRHARAAAWLEGMRTQDLPDDIWREGDDMKR
RTDEDFRPVGDGYDTKDTVRRVKAILVGSTGNLIEWYDVYVFAAFQLYFAPSFFADQSPARQQIFASIVFALGFLARPFG
SLLFGAIADRYGRRISLTASVLLMCFGSLIIACTPSATQIGIAAPILLTVARLLQGLSQGGEYGASATYLSEMSHPNRRG
FYSGVWYVTLIGGQLMAVFTLLIMQKLFLSEDQIRAWGWRIPFFIGAALAVYSFTMRRDMPETDLFNKAKSVAKERAPLA
DLMRHWKSLLLVVGVTIGGTSAFYTYTTYMQKFLKQSVGLTADQTTMVTVGTLIVALIMQPLYGSISDKIGRKPLLIYFG
VMGTLFTYPLLNTIQTTKDPWMAFLLICGGWAIVSGYTSITAIIKAELFPTSVRAMGVGFPYAVTVAVFGGTVDSVAQIF
KFELHWEQGFYWYATACIFVSLLVYIGLKDTKKTSRMDEAH

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=423, Percent_Identity=45.3900709219858, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=29.9765807962529, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1788292, Length=428, Percent_Identity=27.5700934579439, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789941, Length=398, Percent_Identity=28.643216080402, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI87082231, Length=210, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082404, Length=324, Percent_Identity=23.7654320987654, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 57914; Mature: 57914

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLTVIPCCRSVFRVRVFTASLLGGRCGSSAPTGPQTPRDQRERRGPDGRHARAAAWLE
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
GMRTQDLPDDIWREGDDMKRRTDEDFRPVGDGYDTKDTVRRVKAILVGSTGNLIEWYDVY
CCCCCCCCHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VFAAFQLYFAPSFFADQSPARQQIFASIVFALGFLARPFGSLLFGAIADRYGRRISLTAS
HHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHH
VLLMCFGSLIIACTPSATQIGIAAPILLTVARLLQGLSQGGEYGASATYLSEMSHPNRRG
HHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCC
FYSGVWYVTLIGGQLMAVFTLLIMQKLFLSEDQIRAWGWRIPFFIGAALAVYSFTMRRDM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCC
PETDLFNKAKSVAKERAPLADLMRHWKSLLLVVGVTIGGTSAFYTYTTYMQKFLKQSVGL
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
TADQTTMVTVGTLIVALIMQPLYGSISDKIGRKPLLIYFGVMGTLFTYPLLNTIQTTKDP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
WMAFLLICGGWAIVSGYTSITAIIKAELFPTSVRAMGVGFPYAVTVAVFGGTVDSVAQIF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHH
KFELHWEQGFYWYATACIFVSLLVYIGLKDTKKTSRMDEAH
HHHEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCC
>Mature Secondary Structure
MRRLTVIPCCRSVFRVRVFTASLLGGRCGSSAPTGPQTPRDQRERRGPDGRHARAAAWLE
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
GMRTQDLPDDIWREGDDMKRRTDEDFRPVGDGYDTKDTVRRVKAILVGSTGNLIEWYDVY
CCCCCCCCHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VFAAFQLYFAPSFFADQSPARQQIFASIVFALGFLARPFGSLLFGAIADRYGRRISLTAS
HHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHH
VLLMCFGSLIIACTPSATQIGIAAPILLTVARLLQGLSQGGEYGASATYLSEMSHPNRRG
HHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCC
FYSGVWYVTLIGGQLMAVFTLLIMQKLFLSEDQIRAWGWRIPFFIGAALAVYSFTMRRDM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCC
PETDLFNKAKSVAKERAPLADLMRHWKSLLLVVGVTIGGTSAFYTYTTYMQKFLKQSVGL
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
TADQTTMVTVGTLIVALIMQPLYGSISDKIGRKPLLIYFGVMGTLFTYPLLNTIQTTKDP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
WMAFLLICGGWAIVSGYTSITAIIKAELFPTSVRAMGVGFPYAVTVAVFGGTVDSVAQIF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHH
KFELHWEQGFYWYATACIFVSLLVYIGLKDTKKTSRMDEAH
HHHEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]