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The map label for this gene is lcfB [H]

Identifier: 158423686

GI number: 158423686

Start: 2374543

End: 2375991

Strand: Direct

Name: lcfB [H]

Synonym: AZC_2062

Alternate gene names: 158423686

Gene position: 2374543-2375991 (Clockwise)

Preceding gene: 158423685

Following gene: 158423687

Centisome position: 44.22

GC content: 66.74

Gene sequence:

>1449_bases
ATGACGGATCCCACATGCGCCGGACTGTTTCCCGAGCCGGACGGCACGTCGGATCGCCCGCTGGTGTGGGACGACCGCAG
TGGCGTGTGGCGGACTGCCGATGACATCCGCGGCCTCAGCCTGCGCCTTGCCCAGCGGATTGAACGGCCGGGCAAGCAAC
TCGTCTTTCTCCTCGCCACCAACGCGCCGGAGACGCTGGTCGGCCTGCTGGCAGCGGCAGCGGCCGGGCATGCGGTCGCC
CTGATCGACCCCACCCTGTCGCCCGACAGGCTGGACGATCTGGTGCGGGACTATCGGCCGGAACTGGTGATCGGCACCGG
AGCGGGTGCGCTCTGTCAGCCGGGAAATTGGAATGTCGCGCCGTCCGATGGGGTCTTCCCGGAGATCGCCGCGCGGATCG
GGGCTACAGGCCCATCGGACATCCATCCGGACCTCGGGCTTCTGCTGCTAACCTCCGGCACCACGGGAAGCTCCAAATTC
GTGCGCCTGTCCTGGGCTGCGGTGACGGCCAATGCCCGGCAGATTGCCGCGAGTCTTGCGATTACCCGCGAGAGCGTCGC
GATTGCCCATCTGCCGTTCCATTACTCCTACGGCCTCTCCGTCATCACGTCCCATCTCGCTATGGGCGCGCGCATCGCCT
TCATCGACGATGCGCTCACCTCGCCGTCCTTCTGGGACAAGGTGGCGGACTGCGGCGGCAGCCATTTTCCGGGTGTGCCG
TTCCATTACCGCGTCCTGACCCGGCTCGGCCTTGATCGCGCGCCGTCCAGCGTCACCTGCTTCACGCAGGCCGGCGGCCA
TCTCGAAACGCTGACCCAGACGACGATGATCGCTGTCTGCGAGAAGCGCGGGGCCCGTTTCTACGTGATGTACGGCCAGA
CGGAGGCGTCCCCGCGGATGTCCTGCGTGCCCAGTGACCGGCTGCTGGAGAAGCTCGGCTCCGTCGGGCTGGCAATGCCG
GAGGGGCGTCTCAGCATCATCGATGGCGAGGGACGCCCACTGCCAGCGGGCGAGACCGGCGGCGTGGTCTATGAGGGTCC
CAATGTGATGATGGGCTATGCCACCTGCCGCACGGATCTCTCCCTTGGCGATACCACGGGCGGACGCATCGAGACGGGTG
ACCTCGGTCGTCTGGACGCTGAGGGCTTCCTGTTCCTGTCCGGACGGGCGGCGCGCTTCGCCAAGATCGCCGGATTGCGC
CTCAGTCTCGACGACATGGAGCGGCAACTGGCCGGGCTCGGCACTGTCGCCTGCCTTGATCTCGGGGAGCGGATCGCCGT
GGTGTTCGAGGGCGATGTTCCGGAGGGCGCCAAGGAGCAGGCAAGAACCCTCGCGCTGGCCTGCAAGATTCCCGCAGCGA
CCTTCCAACTCAGGGCCTTGCCCGAGATCCCGCGCAAGACGAGCGGGAAGATGGATTATGCCCGTGCGAAGGAGGCGCTG
AATGTTTGA

Upstream 100 bases:

>100_bases
CGATGTGGTGCTGTTCCTGTGCTCGGACCTCTCCCGCTACGTGACGGGACAGGTGCTGCGCGTGGACGGCGGCATGGTCA
TCTGATCGCGGGGCGCAGTC

Downstream 100 bases:

>100_bases
GCCGCTCCTTCAGGCGTCGCCCTATTCGCTGCGGCAGGCGGAGAAGGAGGCGCGGCTGCTGCCAGGCCTAAACGAGCTGA
CGCGCTTCCACTATGAACAC

Product: AMP-dependent synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTDPTCAGLFPEPDGTSDRPLVWDDRSGVWRTADDIRGLSLRLAQRIERPGKQLVFLLATNAPETLVGLLAAAAAGHAVA
LIDPTLSPDRLDDLVRDYRPELVIGTGAGALCQPGNWNVAPSDGVFPEIAARIGATGPSDIHPDLGLLLLTSGTTGSSKF
VRLSWAAVTANARQIAASLAITRESVAIAHLPFHYSYGLSVITSHLAMGARIAFIDDALTSPSFWDKVADCGGSHFPGVP
FHYRVLTRLGLDRAPSSVTCFTQAGGHLETLTQTTMIAVCEKRGARFYVMYGQTEASPRMSCVPSDRLLEKLGSVGLAMP
EGRLSIIDGEGRPLPAGETGGVVYEGPNVMMGYATCRTDLSLGDTTGGRIETGDLGRLDAEGFLFLSGRAARFAKIAGLR
LSLDDMERQLAGLGTVACLDLGERIAVVFEGDVPEGAKEQARTLALACKIPAATFQLRALPEIPRKTSGKMDYARAKEAL
NV

Sequences:

>Translated_482_residues
MTDPTCAGLFPEPDGTSDRPLVWDDRSGVWRTADDIRGLSLRLAQRIERPGKQLVFLLATNAPETLVGLLAAAAAGHAVA
LIDPTLSPDRLDDLVRDYRPELVIGTGAGALCQPGNWNVAPSDGVFPEIAARIGATGPSDIHPDLGLLLLTSGTTGSSKF
VRLSWAAVTANARQIAASLAITRESVAIAHLPFHYSYGLSVITSHLAMGARIAFIDDALTSPSFWDKVADCGGSHFPGVP
FHYRVLTRLGLDRAPSSVTCFTQAGGHLETLTQTTMIAVCEKRGARFYVMYGQTEASPRMSCVPSDRLLEKLGSVGLAMP
EGRLSIIDGEGRPLPAGETGGVVYEGPNVMMGYATCRTDLSLGDTTGGRIETGDLGRLDAEGFLFLSGRAARFAKIAGLR
LSLDDMERQLAGLGTVACLDLGERIAVVFEGDVPEGAKEQARTLALACKIPAATFQLRALPEIPRKTSGKMDYARAKEAL
NV
>Mature_481_residues
TDPTCAGLFPEPDGTSDRPLVWDDRSGVWRTADDIRGLSLRLAQRIERPGKQLVFLLATNAPETLVGLLAAAAAGHAVAL
IDPTLSPDRLDDLVRDYRPELVIGTGAGALCQPGNWNVAPSDGVFPEIAARIGATGPSDIHPDLGLLLLTSGTTGSSKFV
RLSWAAVTANARQIAASLAITRESVAIAHLPFHYSYGLSVITSHLAMGARIAFIDDALTSPSFWDKVADCGGSHFPGVPF
HYRVLTRLGLDRAPSSVTCFTQAGGHLETLTQTTMIAVCEKRGARFYVMYGQTEASPRMSCVPSDRLLEKLGSVGLAMPE
GRLSIIDGEGRPLPAGETGGVVYEGPNVMMGYATCRTDLSLGDTTGGRIETGDLGRLDAEGFLFLSGRAARFAKIAGLRL
SLDDMERQLAGLGTVACLDLGERIAVVFEGDVPEGAKEQARTLALACKIPAATFQLRALPEIPRKTSGKMDYARAKEALN
V

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1789201, Length=371, Percent_Identity=28.0323450134771, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693145, Length=346, Percent_Identity=26.3005780346821, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788107, Length=368, Percent_Identity=23.9130434782609, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17560308, Length=348, Percent_Identity=25.5747126436782, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=266, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 51159; Mature: 51028

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPTCAGLFPEPDGTSDRPLVWDDRSGVWRTADDIRGLSLRLAQRIERPGKQLVFLLAT
CCCCCCCCCCCCCCCCCCCCEEEECCCCCEECHHHHCCHHHHHHHHHCCCCCEEEEEEEC
NAPETLVGLLAAAAAGHAVALIDPTLSPDRLDDLVRDYRPELVIGTGAGALCQPGNWNVA
CCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
PSDGVFPEIAARIGATGPSDIHPDLGLLLLTSGTTGSSKFVRLSWAAVTANARQIAASLA
CCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEECCHHHHHHHHH
ITRESVAIAHLPFHYSYGLSVITSHLAMGARIAFIDDALTSPSFWDKVADCGGSHFPGVP
EECCCEEEEEECEEHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCC
FHYRVLTRLGLDRAPSSVTCFTQAGGHLETLTQTTMIAVCEKRGARFYVMYGQTEASPRM
HHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
SCVPSDRLLEKLGSVGLAMPEGRLSIIDGEGRPLPAGETGGVVYEGPNVMMGYATCRTDL
CCCCHHHHHHHHCCCCEECCCCCEEEECCCCCCCCCCCCCCEEECCCCEEEEEEEEECCC
SLGDTTGGRIETGDLGRLDAEGFLFLSGRAARFAKIAGLRLSLDDMERQLAGLGTVACLD
CCCCCCCCEEECCCCCCCCCCCEEEECCCCHHHHHHCCCEEEHHHHHHHHHCCCCEEEEC
LGERIAVVFEGDVPEGAKEQARTLALACKIPAATFQLRALPEIPRKTSGKMDYARAKEAL
CCCEEEEEEECCCCCCHHHHHHEEEEEEECCCHHEEEECCCCCCCCCCCCCHHHHHHHHH
NV
CC
>Mature Secondary Structure 
TDPTCAGLFPEPDGTSDRPLVWDDRSGVWRTADDIRGLSLRLAQRIERPGKQLVFLLAT
CCCCCCCCCCCCCCCCCCCEEEECCCCCEECHHHHCCHHHHHHHHHCCCCCEEEEEEEC
NAPETLVGLLAAAAAGHAVALIDPTLSPDRLDDLVRDYRPELVIGTGAGALCQPGNWNVA
CCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
PSDGVFPEIAARIGATGPSDIHPDLGLLLLTSGTTGSSKFVRLSWAAVTANARQIAASLA
CCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEECCHHHHHHHHH
ITRESVAIAHLPFHYSYGLSVITSHLAMGARIAFIDDALTSPSFWDKVADCGGSHFPGVP
EECCCEEEEEECEEHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCC
FHYRVLTRLGLDRAPSSVTCFTQAGGHLETLTQTTMIAVCEKRGARFYVMYGQTEASPRM
HHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
SCVPSDRLLEKLGSVGLAMPEGRLSIIDGEGRPLPAGETGGVVYEGPNVMMGYATCRTDL
CCCCHHHHHHHHCCCCEECCCCCEEEECCCCCCCCCCCCCCEEECCCCEEEEEEEEECCC
SLGDTTGGRIETGDLGRLDAEGFLFLSGRAARFAKIAGLRLSLDDMERQLAGLGTVACLD
CCCCCCCCEEECCCCCCCCCCCEEEECCCCHHHHHHCCCEEEHHHHHHHHHCCCCEEEEC
LGERIAVVFEGDVPEGAKEQARTLALACKIPAATFQLRALPEIPRKTSGKMDYARAKEAL
CCCEEEEEEECCCCCCHHHHHHEEEEEEECCCHHEEEECCCCCCCCCCCCCHHHHHHHHH
NV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]