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The map label for this gene is gatA [H]

Identifier: 158423174

GI number: 158423174

Start: 1762294

End: 1763706

Strand: Reverse

Name: gatA [H]

Synonym: AZC_1550

Alternate gene names: 158423174

Gene position: 1763706-1762294 (Counterclockwise)

Preceding gene: 158423175

Following gene: 158423173

Centisome position: 32.85

GC content: 71.69

Gene sequence:

>1413_bases
GTGACTGATATCGCCACGCTGTCGCTGACCGAGATCGCCGCCGCCATCGCCAACCGGCAGGTCTCCTCCCGCGAGGCGAC
GCAAGCCTGTCTCGACCGCATCGCCGACTGGCAGCCGCACCTCAACGCCTTCATCGCCCTTGAGCCGGACGAGGCCCTCA
AAGCGGCCGACGCCGCCGACGCCGCGCTGGCGAAGGGGGAGAGCGCCGGCCCGCTGCACGGTGTGCCGCTCGCCCACAAG
GACATGTTCTACGAGGCGGGCAAGGTGTGCACCTGCGGCACCGAAATTCGCCGCAGCTTCGTGCCGGACACCAGCTCCAC
GGCGCTGGCCCGCATCAAGGCGGCGGGCACCGTGCGGCTCGGCACGCTGCACATGGTGGAATTCGCCTTCGGGCCCACCG
GCCACAATGTGCACTATGGCGACTGCCACAATCCGTGGAACGTCGCCCATGCACCGGGCGGCTCCTCCTCCGGCTCCGGC
GCGGCGGTGGCGGCGCGGCTCACCTTTGCGGCGCTCGGCTCGGACACCGGCGGCTCGGTGCGCATGCCCGCCCATTTCTG
CGGCGTCACCGGCCTCAAGACCACCGTGGGGCGCGTGAGCCGCTTCGGCGCCATGCCGCTCTCCCACTCGCTGGACACGG
TGGGCACGCTGACCCGCACGGCGGAAGATGCCGCCTTGCTGCTCGGCCTCATGGCCGGCGCCGACCCGGCGGACCCGACG
ACGATCGGCGGCGATCTGCCCGATTATCTCGCCGCCACCAAGGGCGGCCTTGCCGGCCTCACCATCGGCATTCCCGACAG
CTTCTATGTGGACGACATCTCGTCCGACACGGCAGCGGCGATGGACGCGACCATCGCCACGCTGGTGCGCGAGGGCGCCA
ACGTGGTGCGCGTGTCGCTGCCGGACCAGCGCCAGCTCTCCGCCGCCGCGCAGGTGCTGCTCGGGGTAGAGGCGAGCGCC
ATCCATCTGCCCTGGCTGCGCGAGCGGCCGCAGGACTATGGCGCGCAGGTCCGCGCGCGGGTGGAGAATGGCCACGCCTT
CTCCGGCGTCGCCTATCTGGAGGCCCTGCGCTGGCGCGGCCCGGCCCTTGCCGCTCATCTCGCGGCGGTGACGGAGGTGG
ATGCGGTGCTGGCACCCGTCTGCCCCGCGCCCTCTCCGACTCTGGCGGAGACTGACGTGGGCGGCAGCTTCAATGCGGAG
GCCGTCATCCAGGGCATCACGCGCTTCACCCGTCCGGTGAACTATCTCGGCCTGCCGGCGCTGGTGGTGCCCGTCGCCCG
TGCCGCGAGCGGCCTGCCCGTCGGCTTCCAGTTCATCGGCCGCCCGTTCGCGGAGGACGTGCTGCTGCGCATCGGCGCCG
GCTTCCAGCGCGCCACCGATTTCCATCTCGCCACGCCGGAGCTGCCCGCATGA

Upstream 100 bases:

>100_bases
GCCCCGCCGTCGAACTGTTCGCGCCCGTGGCGGCAAGCCTGCCGTTCGACCTTGAGCCCGCCACCTTCCTCGTGACCCAG
CGCCCGGCGGAGACACAGAA

Downstream 100 bases:

>100_bases
GCCAGACCACGGCCGAAGCCCCGCTCGTCGAGATCGACGACCTCACCATCCGCTTCACCGGCGAGCGCACGGTCCATGCG
GTGAACGGCGTGTCCCTCTC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTDIATLSLTEIAAAIANRQVSSREATQACLDRIADWQPHLNAFIALEPDEALKAADAADAALAKGESAGPLHGVPLAHK
DMFYEAGKVCTCGTEIRRSFVPDTSSTALARIKAAGTVRLGTLHMVEFAFGPTGHNVHYGDCHNPWNVAHAPGGSSSGSG
AAVAARLTFAALGSDTGGSVRMPAHFCGVTGLKTTVGRVSRFGAMPLSHSLDTVGTLTRTAEDAALLLGLMAGADPADPT
TIGGDLPDYLAATKGGLAGLTIGIPDSFYVDDISSDTAAAMDATIATLVREGANVVRVSLPDQRQLSAAAQVLLGVEASA
IHLPWLRERPQDYGAQVRARVENGHAFSGVAYLEALRWRGPALAAHLAAVTEVDAVLAPVCPAPSPTLAETDVGGSFNAE
AVIQGITRFTRPVNYLGLPALVVPVARAASGLPVGFQFIGRPFAEDVLLRIGAGFQRATDFHLATPELPA

Sequences:

>Translated_470_residues
MTDIATLSLTEIAAAIANRQVSSREATQACLDRIADWQPHLNAFIALEPDEALKAADAADAALAKGESAGPLHGVPLAHK
DMFYEAGKVCTCGTEIRRSFVPDTSSTALARIKAAGTVRLGTLHMVEFAFGPTGHNVHYGDCHNPWNVAHAPGGSSSGSG
AAVAARLTFAALGSDTGGSVRMPAHFCGVTGLKTTVGRVSRFGAMPLSHSLDTVGTLTRTAEDAALLLGLMAGADPADPT
TIGGDLPDYLAATKGGLAGLTIGIPDSFYVDDISSDTAAAMDATIATLVREGANVVRVSLPDQRQLSAAAQVLLGVEASA
IHLPWLRERPQDYGAQVRARVENGHAFSGVAYLEALRWRGPALAAHLAAVTEVDAVLAPVCPAPSPTLAETDVGGSFNAE
AVIQGITRFTRPVNYLGLPALVVPVARAASGLPVGFQFIGRPFAEDVLLRIGAGFQRATDFHLATPELPA
>Mature_469_residues
TDIATLSLTEIAAAIANRQVSSREATQACLDRIADWQPHLNAFIALEPDEALKAADAADAALAKGESAGPLHGVPLAHKD
MFYEAGKVCTCGTEIRRSFVPDTSSTALARIKAAGTVRLGTLHMVEFAFGPTGHNVHYGDCHNPWNVAHAPGGSSSGSGA
AVAARLTFAALGSDTGGSVRMPAHFCGVTGLKTTVGRVSRFGAMPLSHSLDTVGTLTRTAEDAALLLGLMAGADPADPTT
IGGDLPDYLAATKGGLAGLTIGIPDSFYVDDISSDTAAAMDATIATLVREGANVVRVSLPDQRQLSAAAQVLLGVEASAI
HLPWLRERPQDYGAQVRARVENGHAFSGVAYLEALRWRGPALAAHLAAVTEVDAVLAPVCPAPSPTLAETDVGGSFNAEA
VIQGITRFTRPVNYLGLPALVVPVARAASGLPVGFQFIGRPFAEDVLLRIGAGFQRATDFHLATPELPA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=30.0395256916996, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI195972892, Length=495, Percent_Identity=27.0707070707071, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI166795287, Length=145, Percent_Identity=32.4137931034483, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=410, Percent_Identity=30.2439024390244, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17537465, Length=241, Percent_Identity=31.9502074688797, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=243, Percent_Identity=28.8065843621399, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=313, Percent_Identity=25.8785942492013, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=313, Percent_Identity=25.8785942492013, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=201, Percent_Identity=26.3681592039801, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71990152, Length=210, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=461, Percent_Identity=24.7288503253796, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319685, Length=441, Percent_Identity=27.891156462585, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648113, Length=496, Percent_Identity=30.4435483870968, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI161078093, Length=495, Percent_Identity=26.2626262626263, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24652985, Length=507, Percent_Identity=26.0355029585799, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI19922090, Length=507, Percent_Identity=26.0355029585799, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24652981, Length=507, Percent_Identity=26.0355029585799, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24652983, Length=507, Percent_Identity=26.0355029585799, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24644968, Length=507, Percent_Identity=25.0493096646943, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI45550774, Length=241, Percent_Identity=30.2904564315353, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648435, Length=241, Percent_Identity=30.2904564315353, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648437, Length=241, Percent_Identity=30.2904564315353, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356731, Length=228, Percent_Identity=32.0175438596491, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648441, Length=178, Percent_Identity=31.4606741573034, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648439, Length=178, Percent_Identity=31.4606741573034, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48630; Mature: 48498

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIATLSLTEIAAAIANRQVSSREATQACLDRIADWQPHLNAFIALEPDEALKAADAAD
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHH
AALAKGESAGPLHGVPLAHKDMFYEAGKVCTCGTEIRRSFVPDTSSTALARIKAAGTVRL
HHHHCCCCCCCCCCCCCCHHHHHHHCCCEEECCHHHHHHHCCCCCCHHHHHHHCCCEEEE
GTLHMVEFAFGPTGHNVHYGDCHNPWNVAHAPGGSSSGSGAAVAARLTFAALGSDTGGSV
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCE
RMPAHFCGVTGLKTTVGRVSRFGAMPLSHSLDTVGTLTRTAEDAALLLGLMAGADPADPT
ECCHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TIGGDLPDYLAATKGGLAGLTIGIPDSFYVDDISSDTAAAMDATIATLVREGANVVRVSL
CCCCCCHHHHHHCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
PDQRQLSAAAQVLLGVEASAIHLPWLRERPQDYGAQVRARVENGHAFSGVAYLEALRWRG
CCHHHHHHHHHHHHCCCCCEEECCHHHCCCHHHCCCEEEEECCCCCHHHHHHHHHHHCCC
PALAAHLAAVTEVDAVLAPVCPAPSPTLAETDVGGSFNAEAVIQGITRFTRPVNYLGLPA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCHH
LVVPVARAASGLPVGFQFIGRPFAEDVLLRIGAGFQRATDFHLATPELPA
HHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCHHCCCCEECCCCCCC
>Mature Secondary Structure 
TDIATLSLTEIAAAIANRQVSSREATQACLDRIADWQPHLNAFIALEPDEALKAADAAD
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHH
AALAKGESAGPLHGVPLAHKDMFYEAGKVCTCGTEIRRSFVPDTSSTALARIKAAGTVRL
HHHHCCCCCCCCCCCCCCHHHHHHHCCCEEECCHHHHHHHCCCCCCHHHHHHHCCCEEEE
GTLHMVEFAFGPTGHNVHYGDCHNPWNVAHAPGGSSSGSGAAVAARLTFAALGSDTGGSV
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCE
RMPAHFCGVTGLKTTVGRVSRFGAMPLSHSLDTVGTLTRTAEDAALLLGLMAGADPADPT
ECCHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TIGGDLPDYLAATKGGLAGLTIGIPDSFYVDDISSDTAAAMDATIATLVREGANVVRVSL
CCCCCCHHHHHHCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
PDQRQLSAAAQVLLGVEASAIHLPWLRERPQDYGAQVRARVENGHAFSGVAYLEALRWRG
CCHHHHHHHHHHHHCCCCCEEECCHHHCCCHHHCCCEEEEECCCCCHHHHHHHHHHHCCC
PALAAHLAAVTEVDAVLAPVCPAPSPTLAETDVGGSFNAEAVIQGITRFTRPVNYLGLPA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCHH
LVVPVARAASGLPVGFQFIGRPFAEDVLLRIGAGFQRATDFHLATPELPA
HHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCHHCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA