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The map label for this gene is ruvC

Identifier: 15835403

GI number: 15835403

Start: 933052

End: 933564

Strand: Reverse

Name: ruvC

Synonym: TC0789

Alternate gene names: 15835403

Gene position: 933564-933052 (Counterclockwise)

Preceding gene: 15835404

Following gene: 15835402

Centisome position: 87.01

GC content: 39.57

Gene sequence:

>513_bases
ATGGCGGATTTGATTATGGGGATAGATCCGGGAACTTTGGTTTGTGGATATGCATTCATTCGAGTAGAAAATCGATATCA
GATCCAGCCGCATAGTTTTGGAAAAATCAAACTATCTCAGAAACAGGCGTTGTCTCATAGATATAGACAGCTGTTTACAG
AAATTTCCACAATCCTTCAACGAGAAGCTCCTAAAGCCGTTGTTTTAGAGACTCAGTACGTTCATAAAAATCCTCAGAGT
ACAATTAAACTAGGGATGGCAAGGGGAGTATTATTATTAGCAGCCTCTTTACAAGATATCTCTGTGTTTGAGTATGCGCC
TAACACAGCTAAGAAAGCTGCTGTAGGTAAAGGTAATGCTTCTAAGCAGCAGGTACAGCTTATGGTAAGTAAGCTACTGA
ATATTCAGGATCTGCTAGCGGATGATAATGAGGATATTGCGGATGCTTTTGCTTTAGCCATGTGTCATGCACATTTAGCG
CCTTATCAGGATCTAAAAAAAAGTCTTTTGTAA

Upstream 100 bases:

>100_bases
TTGGGTTCCTTGATAGGTCCTTAATTTCATTAGCAACGCTTTCCTTTATCATGTAAAATTTGCACAACGTGAAAATTAGA
AGACTCGGGTGTAAAAAGGC

Downstream 100 bases:

>100_bases
TCGATGGAATTAGACTCCAATGTATGAGTATATTAAAGGAAAATTAACCCATACTGATGAAGCTTATGTTGTGATTGAAA
GCTTTGGTATAGGCTATTCT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MADLIMGIDPGTLVCGYAFIRVENRYQIQPHSFGKIKLSQKQALSHRYRQLFTEISTILQREAPKAVVLETQYVHKNPQS
TIKLGMARGVLLLAASLQDISVFEYAPNTAKKAAVGKGNASKQQVQLMVSKLLNIQDLLADDNEDIADAFALAMCHAHLA
PYQDLKKSLL

Sequences:

>Translated_170_residues
MADLIMGIDPGTLVCGYAFIRVENRYQIQPHSFGKIKLSQKQALSHRYRQLFTEISTILQREAPKAVVLETQYVHKNPQS
TIKLGMARGVLLLAASLQDISVFEYAPNTAKKAAVGKGNASKQQVQLMVSKLLNIQDLLADDNEDIADAFALAMCHAHLA
PYQDLKKSLL
>Mature_169_residues
ADLIMGIDPGTLVCGYAFIRVENRYQIQPHSFGKIKLSQKQALSHRYRQLFTEISTILQREAPKAVVLETQYVHKNPQST
IKLGMARGVLLLAASLQDISVFEYAPNTAKKAAVGKGNASKQQVQLMVSKLLNIQDLLADDNEDIADAFALAMCHAHLAP
YQDLKKSLL

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=132, Percent_Identity=38.6363636363636, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_CHLMU (Q9PJN9)

Other databases:

- EMBL:   AE002160
- PIR:   E81662
- RefSeq:   NP_297162.1
- ProteinModelPortal:   Q9PJN9
- SMR:   Q9PJN9
- GeneID:   1246155
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0789
- TIGR:   TC_0789
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   Q9PJN9
- ProtClustDB:   PRK00039
- BioCyc:   CMUR243161:TC_0789-MONOMER
- BRENDA:   3.1.22.4
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18837; Mature: 18706

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLIMGIDPGTLVCGYAFIRVENRYQIQPHSFGKIKLSQKQALSHRYRQLFTEISTILQ
CCCCEECCCCCHHHHHHHEEEECCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
REAPKAVVLETQYVHKNPQSTIKLGMARGVLLLAASLQDISVFEYAPNTAKKAAVGKGNA
HCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
SKQQVQLMVSKLLNIQDLLADDNEDIADAFALAMCHAHLAPYQDLKKSLL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
ADLIMGIDPGTLVCGYAFIRVENRYQIQPHSFGKIKLSQKQALSHRYRQLFTEISTILQ
CCCEECCCCCHHHHHHHEEEECCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
REAPKAVVLETQYVHKNPQSTIKLGMARGVLLLAASLQDISVFEYAPNTAKKAAVGKGNA
HCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
SKQQVQLMVSKLLNIQDLLADDNEDIADAFALAMCHAHLAPYQDLKKSLL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935