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The map label for this gene is pnpA

Identifier: 15834850

GI number: 15834850

Start: 271947

End: 274028

Strand: Reverse

Name: pnpA

Synonym: TC0230

Alternate gene names: 15834850

Gene position: 274028-271947 (Counterclockwise)

Preceding gene: 15834851

Following gene: 15834847

Centisome position: 25.54

GC content: 42.27

Gene sequence:

>2082_bases
ATGGCTTTTGAGACTTTTTCTGTTGCGTTAGACAAAGATAAGACACTGATTTTCGAGACAGGGAAAATAGCTCGCCAAGC
CAATGGAGCTGTTCTTGTCAAAATGAATGAAACTTGGGTTTTTTCATCAGCGTGCGCGGCCTCTTTATCAGAGGCCGTAG
ATTTTCTGCCTTTCAGAGTAGACTATCAAGAAAAGTTTTCCTCCGCGGGGAAAACCTCTGGAGGATTTCTGAAACGCGAA
GGTCGTCCCTCCGAGAAAGAAATTCTTATTTCTCGACTGATAGATCGCTCTTTGCGTCCATCATTCCCTAATAGACTTAT
GCAAGACATTCAAGTCTTGTCCTACGTTTGGTCTTATGACGGGAAAACTTTACCAGACCCCTTAGCTATTTGTGGAGCTT
CTGCAGCTTTAGCTATCTCAGAGGTCCCTCAGAATTGTATTGTTGCAGGTGTACGCGTCGGTCTCGTCGAAGGAAAATGG
GTAGTTAACCCAACTAAAGATGAGTTAGACGCATCCAAGCTTGATCTCGTTATGGCAGGAACAGCTTCTGCGGTATTAAT
GATCGAAGGTCATTGCGACTTTTTGACTGAAGAGCAAGTTTTAGAAGCTATTGCTTTTGGACAAAAATACATAGCTAAAA
TATGTGACGCTATTGAAGCTTGGCAGAAAGCGATTGGTAAGGAAAAACAGCTCTCGGCTGTCCTTGATCTGCCGGAAGAT
GTGCAAAATGTTGTTTCGAATTTTATTAGAGAAAAATTCGAAAAAGCACTGTCTTTTAGAGATAGAGACGCTTTAGAACA
AGTCTCAAAAGAGTTAGAAGAATCTGTTGTCGCCAACTTAGTTCAAGAAGAAAGTGATTTTTCTTTATTGAACGTGAAAG
CTGCATTTAAGAATGCAAAATCCAATCAAATGCGAGCTTTAATTAGAGATCTCGGCATTCGCGTAGACGGACGAAGTACA
ACAGAAATTCGCCCTATTTCCATAGAAGTCTCTTTCCTTCCACGAACACATGGAAGCTGCCTATTTACCCGTGGAGAAAC
ACAAAGTGTAGCTGTATGCACACTTGGGGGTGAAAGTATGGCACAACGATTTGAAGACCTAAATGGGGATGGAGCAGCGC
GCTTTTATTTGCAGTATTTTTTCCCTCCTTTCTCTGTAGGAGAAGTTGGTAGAATCGGATCTCCAGGGAGACGCGAAATA
GGTCACGGAAAATTAGCTGAAAAAGCTTTAAGTCATGTCCTTCCTGAAGCATCTCGATTCCCTTACACTGTTCGAGTGGA
GTCTAATATTACAGAATCCAATGGGTCTTCTTCTATGGCTTCTGTATGTGGGGGCTGTCTTTCTCTGATGGATGCAGGAG
TGCCTATCAAAGCTCCTGTAGCCGGCATTGCTATGGGTCTAATCCTAGATCAAGATAAGGCTATTGTCCTTTCTGATATT
TCAGGGATAGAAGATCATCTCGGCGATATGGATTTTAAAGTCGCGGGAACTGAAGAAGGAATCACAGCATTCCAAATGGA
CATTAAAGTAGAAGGAATTACCCATGAGATCATGGAGCAGGCGTTGGCCCAAGCTAAGCAAGGTCGAAGCCATATCCTAA
ATCTTATGACACAGGTAATGTCTTCTCCTAATGATTCTGTTTCCAGATATGCTCCTCGTATTGAAACTATGCAAATCAAT
ACTTCAAAAATCGCGACAGTAATAGGTCCTGGAGGGAAACAGATCCGTCAAATTATTGAGCGATCAGGAGCTCAAGTTGA
CATTAACGATAACGGGCTCATTAACATATCAGCTAACACCCAAGAATCTATCGATAAAGCTAAGGAGCTTATTGAGGGAT
TAACGGGAGAAGTTGAGGTTGGCAAGATCTATAACGGCCGAGTCACTTCCGTTGTGGCCTTTGGAGCATTTGTAGAAGTC
CTTCCTGGTAAAGAGGGTCTTTGTCATATTTCAGAATTGTCTAAACAAAAAGTAGACAATGTAGCTGATTTCGTAAAAGA
AGGTGATCGCCTAGCGGTTAAGTTGCTCAGCATCAATGAGAAGGGGCAACTAAAACTTAGCCATAAAGCAACTTTAGAAT
AG

Upstream 100 bases:

>100_bases
CTTAGAGTACTTAAATTCTACTGATACTGAAAGATATAAAAATTTAATCGCTCGCCTTAACTTGAGAAAATAATACCCGA
TTTCGTTTTAGGACAACTTC

Downstream 100 bases:

>100_bases
TAAAATAGACGATCCTACTAAATTCAAATAACTCTGAAAAGCTAAGAGAGGATTCTCTCTTAGCTTTTTTATTGATGAAA
CTTTATTGCTTATCTCTTAC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 693; Mature: 692

Protein sequence:

>693_residues
MAFETFSVALDKDKTLIFETGKIARQANGAVLVKMNETWVFSSACAASLSEAVDFLPFRVDYQEKFSSAGKTSGGFLKRE
GRPSEKEILISRLIDRSLRPSFPNRLMQDIQVLSYVWSYDGKTLPDPLAICGASAALAISEVPQNCIVAGVRVGLVEGKW
VVNPTKDELDASKLDLVMAGTASAVLMIEGHCDFLTEEQVLEAIAFGQKYIAKICDAIEAWQKAIGKEKQLSAVLDLPED
VQNVVSNFIREKFEKALSFRDRDALEQVSKELEESVVANLVQEESDFSLLNVKAAFKNAKSNQMRALIRDLGIRVDGRST
TEIRPISIEVSFLPRTHGSCLFTRGETQSVAVCTLGGESMAQRFEDLNGDGAARFYLQYFFPPFSVGEVGRIGSPGRREI
GHGKLAEKALSHVLPEASRFPYTVRVESNITESNGSSSMASVCGGCLSLMDAGVPIKAPVAGIAMGLILDQDKAIVLSDI
SGIEDHLGDMDFKVAGTEEGITAFQMDIKVEGITHEIMEQALAQAKQGRSHILNLMTQVMSSPNDSVSRYAPRIETMQIN
TSKIATVIGPGGKQIRQIIERSGAQVDINDNGLINISANTQESIDKAKELIEGLTGEVEVGKIYNGRVTSVVAFGAFVEV
LPGKEGLCHISELSKQKVDNVADFVKEGDRLAVKLLSINEKGQLKLSHKATLE

Sequences:

>Translated_693_residues
MAFETFSVALDKDKTLIFETGKIARQANGAVLVKMNETWVFSSACAASLSEAVDFLPFRVDYQEKFSSAGKTSGGFLKRE
GRPSEKEILISRLIDRSLRPSFPNRLMQDIQVLSYVWSYDGKTLPDPLAICGASAALAISEVPQNCIVAGVRVGLVEGKW
VVNPTKDELDASKLDLVMAGTASAVLMIEGHCDFLTEEQVLEAIAFGQKYIAKICDAIEAWQKAIGKEKQLSAVLDLPED
VQNVVSNFIREKFEKALSFRDRDALEQVSKELEESVVANLVQEESDFSLLNVKAAFKNAKSNQMRALIRDLGIRVDGRST
TEIRPISIEVSFLPRTHGSCLFTRGETQSVAVCTLGGESMAQRFEDLNGDGAARFYLQYFFPPFSVGEVGRIGSPGRREI
GHGKLAEKALSHVLPEASRFPYTVRVESNITESNGSSSMASVCGGCLSLMDAGVPIKAPVAGIAMGLILDQDKAIVLSDI
SGIEDHLGDMDFKVAGTEEGITAFQMDIKVEGITHEIMEQALAQAKQGRSHILNLMTQVMSSPNDSVSRYAPRIETMQIN
TSKIATVIGPGGKQIRQIIERSGAQVDINDNGLINISANTQESIDKAKELIEGLTGEVEVGKIYNGRVTSVVAFGAFVEV
LPGKEGLCHISELSKQKVDNVADFVKEGDRLAVKLLSINEKGQLKLSHKATLE
>Mature_692_residues
AFETFSVALDKDKTLIFETGKIARQANGAVLVKMNETWVFSSACAASLSEAVDFLPFRVDYQEKFSSAGKTSGGFLKREG
RPSEKEILISRLIDRSLRPSFPNRLMQDIQVLSYVWSYDGKTLPDPLAICGASAALAISEVPQNCIVAGVRVGLVEGKWV
VNPTKDELDASKLDLVMAGTASAVLMIEGHCDFLTEEQVLEAIAFGQKYIAKICDAIEAWQKAIGKEKQLSAVLDLPEDV
QNVVSNFIREKFEKALSFRDRDALEQVSKELEESVVANLVQEESDFSLLNVKAAFKNAKSNQMRALIRDLGIRVDGRSTT
EIRPISIEVSFLPRTHGSCLFTRGETQSVAVCTLGGESMAQRFEDLNGDGAARFYLQYFFPPFSVGEVGRIGSPGRREIG
HGKLAEKALSHVLPEASRFPYTVRVESNITESNGSSSMASVCGGCLSLMDAGVPIKAPVAGIAMGLILDQDKAIVLSDIS
GIEDHLGDMDFKVAGTEEGITAFQMDIKVEGITHEIMEQALAQAKQGRSHILNLMTQVMSSPNDSVSRYAPRIETMQINT
SKIATVIGPGGKQIRQIIERSGAQVDINDNGLINISANTQESIDKAKELIEGLTGEVEVGKIYNGRVTSVVAFGAFVEVL
PGKEGLCHISELSKQKVDNVADFVKEGDRLAVKLLSINEKGQLKLSHKATLE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=713, Percent_Identity=35.203366058906, Blast_Score=411, Evalue=1e-114,
Organism=Escherichia coli, GI145693187, Length=685, Percent_Identity=45.985401459854, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI157672248, Length=219, Percent_Identity=25.1141552511416, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI115534063, Length=709, Percent_Identity=32.0169252468265, Blast_Score=312, Evalue=4e-85,
Organism=Drosophila melanogaster, GI281362905, Length=710, Percent_Identity=37.0422535211268, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=710, Percent_Identity=37.0422535211268, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=710, Percent_Identity=37.0422535211268, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=649, Percent_Identity=37.5963020030817, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 75335; Mature: 75204

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFETFSVALDKDKTLIFETGKIARQANGAVLVKMNETWVFSSACAASLSEAVDFLPFRV
CCCEEEEEEECCCCEEEEECCHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCEEE
DYQEKFSSAGKTSGGFLKREGRPSEKEILISRLIDRSLRPSFPNRLMQDIQVLSYVWSYD
CHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
GKTLPDPLAICGASAALAISEVPQNCIVAGVRVGLVEGKWVVNPTKDELDASKLDLVMAG
CCCCCCHHHHHCCHHHHHHHHCCHHHHHHHHEEEEEECEEEECCCHHHCCHHHEEEEEEC
TASAVLMIEGHCDFLTEEQVLEAIAFGQKYIAKICDAIEAWQKAIGKEKQLSAVLDLPED
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
VQNVVSNFIREKFEKALSFRDRDALEQVSKELEESVVANLVQEESDFSLLNVKAAFKNAK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCC
SNQMRALIRDLGIRVDGRSTTEIRPISIEVSFLPRTHGSCLFTRGETQSVAVCTLGGESM
HHHHHHHHHHCCEEECCCCCCEEEEEEEEEEEECCCCCCEEEECCCCCEEEEEEECCHHH
AQRFEDLNGDGAARFYLQYFFPPFSVGEVGRIGSPGRREIGHGKLAEKALSHVLPEASRF
HHHHHHCCCCCHHEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
PYTVRVESNITESNGSSSMASVCGGCLSLMDAGVPIKAPVAGIAMGLILDQDKAIVLSDI
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEEEECC
SGIEDHLGDMDFKVAGTEEGITAFQMDIKVEGITHEIMEQALAQAKQGRSHILNLMTQVM
CCHHHHHCCCCEEEECCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SSPNDSVSRYAPRIETMQINTSKIATVIGPGGKQIRQIIERSGAQVDINDNGLINISANT
CCCCCHHHHHCCCCEEEEECHHHEEEEECCCHHHHHHHHHHCCCEEEECCCCEEEEECCC
QESIDKAKELIEGLTGEVEVGKIYNGRVTSVVAFGAFVEVLPGKEGLCHISELSKQKVDN
HHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
VADFVKEGDRLAVKLLSINEKGQLKLSHKATLE
HHHHHHCCCEEEEEEEEECCCCCEEECCCCCCC
>Mature Secondary Structure 
AFETFSVALDKDKTLIFETGKIARQANGAVLVKMNETWVFSSACAASLSEAVDFLPFRV
CCEEEEEEECCCCEEEEECCHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCEEE
DYQEKFSSAGKTSGGFLKREGRPSEKEILISRLIDRSLRPSFPNRLMQDIQVLSYVWSYD
CHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
GKTLPDPLAICGASAALAISEVPQNCIVAGVRVGLVEGKWVVNPTKDELDASKLDLVMAG
CCCCCCHHHHHCCHHHHHHHHCCHHHHHHHHEEEEEECEEEECCCHHHCCHHHEEEEEEC
TASAVLMIEGHCDFLTEEQVLEAIAFGQKYIAKICDAIEAWQKAIGKEKQLSAVLDLPED
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
VQNVVSNFIREKFEKALSFRDRDALEQVSKELEESVVANLVQEESDFSLLNVKAAFKNAK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCC
SNQMRALIRDLGIRVDGRSTTEIRPISIEVSFLPRTHGSCLFTRGETQSVAVCTLGGESM
HHHHHHHHHHCCEEECCCCCCEEEEEEEEEEEECCCCCCEEEECCCCCEEEEEEECCHHH
AQRFEDLNGDGAARFYLQYFFPPFSVGEVGRIGSPGRREIGHGKLAEKALSHVLPEASRF
HHHHHHCCCCCHHEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
PYTVRVESNITESNGSSSMASVCGGCLSLMDAGVPIKAPVAGIAMGLILDQDKAIVLSDI
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEEEECC
SGIEDHLGDMDFKVAGTEEGITAFQMDIKVEGITHEIMEQALAQAKQGRSHILNLMTQVM
CCHHHHHCCCCEEEECCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SSPNDSVSRYAPRIETMQINTSKIATVIGPGGKQIRQIIERSGAQVDINDNGLINISANT
CCCCCHHHHHCCCCEEEEECHHHEEEEECCCHHHHHHHHHHCCCEEEECCCCEEEEECCC
QESIDKAKELIEGLTGEVEVGKIYNGRVTSVVAFGAFVEVLPGKEGLCHISELSKQKVDN
HHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
VADFVKEGDRLAVKLLSINEKGQLKLSHKATLE
HHHHHHCCCEEEEEEEEECCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA