Click here to switch to the map view.

The map label for this gene is cusS [H]

Identifier: 158340378

GI number: 158340378

Start: 216623

End: 218092

Strand: Direct

Name: cusS [H]

Synonym: AM1_C0305

Alternate gene names: 158340378

Gene position: 216623-218092 (Clockwise)

Preceding gene: 158340377

Following gene: 158340379

Centisome position: 79.31

GC content: 45.99

Gene sequence:

>1470_bases
ATGGCAATATCCAAACAGCAAAAACATCTACTGATTATTCCCAATCGCTCACTATGGGGAAGTTTTTTTCGCCAAACCCG
GACACGTATTTTACTGATGTACGTTGTCCTGCTGAGTCTATTCACAGGACTGGCAATTCCTCTATTTCAGGTCTTGGTTA
CAGCTCAGGTTTCTCGACGAGTGCGGAGTGATCTCAAAGGAGCCAGAGAAGTTTTCCTGGAAGCCTATACCGATTGGGAA
CAACAACCCAATCAAACGAAGCGTGATCTAGAAGACTTTGCAGATCAGTTTTTATCAAATCAAATCCCCGAAGATGATAG
CTTCTTCATCTTTATCATTGATCAAGATCTCTATCGCTCAAATCCACCGCGATTATTGGAACCAATGCGGCCAGGGTCTG
AGCTTTATCAACAATGGGCCTCTGTGACTCAATTTAGTCGAGGAGAGTGGAAGACAGCAAATACGGATCTAGGCAACATT
CTTTATAAAGCAGAGCCGCTGATCGTAGATGGTGAACAACTAGGAATTATGGTGCTTGCTCATGATACGGCAGGAGAGCA
ACAAGAATCTTTAGCCAGCGTTTATACCTTCACCTTAATTGAAGGAGGGGTTGTATGTATTTCTCTCTTGTTGGCTTGGT
TCGCGACAGGGAGGTTACTCAAACCTGTTCGGGAGCTGGCCATTGCTGCTCGCAAAATTAATGAATCTGATTTAGACCTG
CGTTTGTCCCCACAGGGGTCAGGAGAATTGTCAGACCTAGCCCAAACCTTCAATGCGATGATGGATAGACTTCAAGATAG
TTTTAATAGCCAGCGTCGCTTTGTGAACGATGCGGGCCATGAACTCCGTACCCCGATTACAGTTATTCGCGGGCATCTGG
AACTGATTGGAGATGACCCCCAAGAACAGCAAGAAACGATTGAACTGGTTCTAGATGAACTGGATCGGATGGGTCGGTTA
GTGAATGACATGGTTGCCTTAGCCAAGTCTGAACACCCCGACTTTCTTCAATATGAAATGATTGATCTCAAGCAATTCAT
CTACGATTTGCATACCAAAGCCCAAGCCATAGCAAAGCACAATTGGCAGCTCACCCTGAAATGCTCTGGCTCAATGATGG
GTGACCGTCAGAGGCTGACAGGAGCCCTACTCAATCTCCTGAGAAATGCTGCCCAACATACCCCATCCACAGGAACCATT
GAGTTAGGTTGTAAACAAACCCTTACTCAAGTTGTTTTCTGGGTTAAGGATACTGGGAAGGGGATTGCTCTAGAAGATCA
ACAGCGAATTTTTGACCGTTTTGCTCGCTTAGGCCAACGCCAATCGGAAGGTTCCGGCCTGGGATTAGCCATTGTTCAAG
CTTTTACCAAAGCCCACGGAGGTAAAATTGAGCTGATGAGTCAACTAGGTGTCGGCTCTACCTTTAGAATTACGCTGCCC
CTCTCTGCCCAAAATAGCACTGTGGTATGA

Upstream 100 bases:

>100_bases
GTCTATGTGGGCTATCTACGCAAAAAGCTGGGTGACGATCTGATTCAGACTGTACGAGGGGTTGGGTATCGGCTAAATGC
TTAGTCTAGATGAAGACGTT

Downstream 100 bases:

>100_bases
AAAACGTTCAATCTTTCTCCTGGGTACACCCAGTTCCAGTAGCCTGAGCAGACAGCAGATAGAGTCCTTACCACCCGAGA
AGGCAACCAAGATACGGTCA

Product: two-component sensor histidine kinase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MAISKQQKHLLIIPNRSLWGSFFRQTRTRILLMYVVLLSLFTGLAIPLFQVLVTAQVSRRVRSDLKGAREVFLEAYTDWE
QQPNQTKRDLEDFADQFLSNQIPEDDSFFIFIIDQDLYRSNPPRLLEPMRPGSELYQQWASVTQFSRGEWKTANTDLGNI
LYKAEPLIVDGEQLGIMVLAHDTAGEQQESLASVYTFTLIEGGVVCISLLLAWFATGRLLKPVRELAIAARKINESDLDL
RLSPQGSGELSDLAQTFNAMMDRLQDSFNSQRRFVNDAGHELRTPITVIRGHLELIGDDPQEQQETIELVLDELDRMGRL
VNDMVALAKSEHPDFLQYEMIDLKQFIYDLHTKAQAIAKHNWQLTLKCSGSMMGDRQRLTGALLNLLRNAAQHTPSTGTI
ELGCKQTLTQVVFWVKDTGKGIALEDQQRIFDRFARLGQRQSEGSGLGLAIVQAFTKAHGGKIELMSQLGVGSTFRITLP
LSAQNSTVV

Sequences:

>Translated_489_residues
MAISKQQKHLLIIPNRSLWGSFFRQTRTRILLMYVVLLSLFTGLAIPLFQVLVTAQVSRRVRSDLKGAREVFLEAYTDWE
QQPNQTKRDLEDFADQFLSNQIPEDDSFFIFIIDQDLYRSNPPRLLEPMRPGSELYQQWASVTQFSRGEWKTANTDLGNI
LYKAEPLIVDGEQLGIMVLAHDTAGEQQESLASVYTFTLIEGGVVCISLLLAWFATGRLLKPVRELAIAARKINESDLDL
RLSPQGSGELSDLAQTFNAMMDRLQDSFNSQRRFVNDAGHELRTPITVIRGHLELIGDDPQEQQETIELVLDELDRMGRL
VNDMVALAKSEHPDFLQYEMIDLKQFIYDLHTKAQAIAKHNWQLTLKCSGSMMGDRQRLTGALLNLLRNAAQHTPSTGTI
ELGCKQTLTQVVFWVKDTGKGIALEDQQRIFDRFARLGQRQSEGSGLGLAIVQAFTKAHGGKIELMSQLGVGSTFRITLP
LSAQNSTVV
>Mature_488_residues
AISKQQKHLLIIPNRSLWGSFFRQTRTRILLMYVVLLSLFTGLAIPLFQVLVTAQVSRRVRSDLKGAREVFLEAYTDWEQ
QPNQTKRDLEDFADQFLSNQIPEDDSFFIFIIDQDLYRSNPPRLLEPMRPGSELYQQWASVTQFSRGEWKTANTDLGNIL
YKAEPLIVDGEQLGIMVLAHDTAGEQQESLASVYTFTLIEGGVVCISLLLAWFATGRLLKPVRELAIAARKINESDLDLR
LSPQGSGELSDLAQTFNAMMDRLQDSFNSQRRFVNDAGHELRTPITVIRGHLELIGDDPQEQQETIELVLDELDRMGRLV
NDMVALAKSEHPDFLQYEMIDLKQFIYDLHTKAQAIAKHNWQLTLKCSGSMMGDRQRLTGALLNLLRNAAQHTPSTGTIE
LGCKQTLTQVVFWVKDTGKGIALEDQQRIFDRFARLGQRQSEGSGLGLAIVQAFTKAHGGKIELMSQLGVGSTFRITLPL
SAQNSTVV

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=289, Percent_Identity=34.6020761245675, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788393, Length=272, Percent_Identity=32.3529411764706, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786600, Length=260, Percent_Identity=30.7692307692308, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789403, Length=269, Percent_Identity=33.0855018587361, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=30.6034482758621, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790346, Length=271, Percent_Identity=29.8892988929889, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788549, Length=216, Percent_Identity=31.4814814814815, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788713, Length=261, Percent_Identity=29.5019157088123, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788279, Length=287, Percent_Identity=26.4808362369338, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787374, Length=295, Percent_Identity=23.3898305084746, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790861, Length=365, Percent_Identity=26.027397260274, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787894, Length=270, Percent_Identity=26.2962962962963, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693157, Length=249, Percent_Identity=29.718875502008, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=29.6137339055794, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789808, Length=305, Percent_Identity=23.6065573770492, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790551, Length=287, Percent_Identity=26.4808362369338, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=358, Percent_Identity=24.0223463687151, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082128, Length=293, Percent_Identity=24.2320819112628, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55126; Mature: 54995

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISKQQKHLLIIPNRSLWGSFFRQTRTRILLMYVVLLSLFTGLAIPLFQVLVTAQVSRR
CCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRSDLKGAREVFLEAYTDWEQQPNQTKRDLEDFADQFLSNQIPEDDSFFIFIIDQDLYRS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHCC
NPPRLLEPMRPGSELYQQWASVTQFSRGEWKTANTDLGNILYKAEPLIVDGEQLGIMVLA
CCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCEEECCCCEEEEEEE
HDTAGEQQESLASVYTFTLIEGGVVCISLLLAWFATGRLLKPVRELAIAARKINESDLDL
ECCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCE
RLSPQGSGELSDLAQTFNAMMDRLQDSFNSQRRFVNDAGHELRTPITVIRGHLELIGDDP
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHCCCCC
QEQQETIELVLDELDRMGRLVNDMVALAKSEHPDFLQYEMIDLKQFIYDLHTKAQAIAKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NWQLTLKCSGSMMGDRQRLTGALLNLLRNAAQHTPSTGTIELGCKQTLTQVVFWVKDTGK
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCC
GIALEDQQRIFDRFARLGQRQSEGSGLGLAIVQAFTKAHGGKIELMSQLGVGSTFRITLP
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHCCCCCEEEEEEE
LSAQNSTVV
ECCCCCCCC
>Mature Secondary Structure 
AISKQQKHLLIIPNRSLWGSFFRQTRTRILLMYVVLLSLFTGLAIPLFQVLVTAQVSRR
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRSDLKGAREVFLEAYTDWEQQPNQTKRDLEDFADQFLSNQIPEDDSFFIFIIDQDLYRS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHCC
NPPRLLEPMRPGSELYQQWASVTQFSRGEWKTANTDLGNILYKAEPLIVDGEQLGIMVLA
CCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCEEECCCCEEEEEEE
HDTAGEQQESLASVYTFTLIEGGVVCISLLLAWFATGRLLKPVRELAIAARKINESDLDL
ECCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCE
RLSPQGSGELSDLAQTFNAMMDRLQDSFNSQRRFVNDAGHELRTPITVIRGHLELIGDDP
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHCCCCC
QEQQETIELVLDELDRMGRLVNDMVALAKSEHPDFLQYEMIDLKQFIYDLHTKAQAIAKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NWQLTLKCSGSMMGDRQRLTGALLNLLRNAAQHTPSTGTIELGCKQTLTQVVFWVKDTGK
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCC
GIALEDQQRIFDRFARLGQRQSEGSGLGLAIVQAFTKAHGGKIELMSQLGVGSTFRITLP
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHCCCCCEEEEEEE
LSAQNSTVV
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]