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The map label for this gene is cefD [H]

Identifier: 157963460

GI number: 157963460

Start: 4435713

End: 4437098

Strand: Direct

Name: cefD [H]

Synonym: Spea_3648

Alternate gene names: 157963460

Gene position: 4435713-4437098 (Clockwise)

Preceding gene: 157963458

Following gene: 157963469

Centisome position: 85.72

GC content: 46.61

Gene sequence:

>1386_bases
ATGAACCACAAACCTGAAGCCAACTTGGCTAACGCTGGACGTCGAGGCTTTTTAAAGACAACCACTGGCGTTGCACTTGC
TGCTGTCGGAACAAGTTTACTGCCAGCCACTGCACATGCGAGCAGTGACAATCATATTGAGCCTTTCCATGGTCATGGCT
CTGGCCAGGGCTTCTGGAACAAGGTTCGCAATGAATTTATCCTAAACAAGCGCACTACCTACATGAACATTGGTACTGCT
GGCACCATGCCAAAGCGTGTCTTAAAAGACTATAACCGCACCAATGAACAGATCTCTATTAATCCTTGGAACAGCAATAT
TCCAACTTTAGATTATGCCAAGCAGATAGCTCCAAGCTTCGGTGCCGATGAGCACGAATTAGTGCTATGTCGTAACACTA
CCGATGGCTTATGTACCATTATCAACGGCCTACAGTTTGAGTACGGCGACATCATCTTAACCACTAACCACGAACACCAC
GGTGTCACTACACCACTAAAGCATGTAGCACAGCGCTATGGCGCCGAAGTGATCGAGTTGGCTCTACCTGTTTACACGCC
AAATAATGAAGTAAGCAGTGATGATTTCGTGCAGGTCTTTAACGATGCGGTAAGCCAATATGGCAATCGCGTACGCTTGA
TGGTGTTCTCTCACGTGACGTTCACCACTGGTACCACGCTTCCAGCTAAGCGTATCTGCCAAGAGGTCGCCATTCCAAAT
GGTATCATTACCTTAGTCGATGGTGCGCATACTCCCGGTATGTTCAACCTAGATTTCCATGATATCGATTGTGACTTCTA
CTCAGGTGCAGGCCACAAGTGGCAATGTGGTCCTGGTGCTACAGGCTTCCTATACGTACGTGATAACGCTAATCGTCTAT
TCGAATTCTGGAGCGATCGCGAAACACCGCTATGGTTTATCAACACCTCATCATCGGCTGCAGAAGTACAGACTAAGCTG
CAATCTGTTGGCCAGGATAACTACCCAGCCAAACGCGCTCTAGCAGATGCTTGCGCACTTTGGGATGAGATTGGTCGTGA
TGCAATTGAAGAGCGCATTCTAGATTTAAGCGCACTATGTAAGAAACAACTAGCCAAGAAATTCCCTGAGGCGATGATCT
TTGCTCCTAATATTCGTGAGCTATCTAGCGGCCTAACTACCTTCAACCCATTCGACGATCTAAATGATGGCGAGCTACTG
ACAGAGTTCCGCGACCGTTTACATGAAGAGTATGGCTACATAGTTAGAACCACCTCTTTCTACTTAGATCAGTACGATAC
GGTTAAGACTTACTCTCTACGCATTTCGACTCACCTTTTCCATGATGAAGATGATGTTAAGGGTCTAGTCAAAGCGATGA
AGCAACTGCATCGTGAAATGAGATAA

Upstream 100 bases:

>100_bases
AATCAAACCTAGCAAACCGCTTTAACCACCAACAAACAAAAATATAAAATAATAAATATAAATCATTATTTAAAAACATA
AAAAATAACAAGGAAAAACA

Downstream 100 bases:

>100_bases
GCCATAAAATAAGCACCAATAGAAACGGCCCAGTTGTTATGATTTAAATAACAACCTGGGCCGTTTTTTTATCTTGAAAT
CGAGCAAAAATGCTTACAAA

Product: class V aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MNHKPEANLANAGRRGFLKTTTGVALAAVGTSLLPATAHASSDNHIEPFHGHGSGQGFWNKVRNEFILNKRTTYMNIGTA
GTMPKRVLKDYNRTNEQISINPWNSNIPTLDYAKQIAPSFGADEHELVLCRNTTDGLCTIINGLQFEYGDIILTTNHEHH
GVTTPLKHVAQRYGAEVIELALPVYTPNNEVSSDDFVQVFNDAVSQYGNRVRLMVFSHVTFTTGTTLPAKRICQEVAIPN
GIITLVDGAHTPGMFNLDFHDIDCDFYSGAGHKWQCGPGATGFLYVRDNANRLFEFWSDRETPLWFINTSSSAAEVQTKL
QSVGQDNYPAKRALADACALWDEIGRDAIEERILDLSALCKKQLAKKFPEAMIFAPNIRELSSGLTTFNPFDDLNDGELL
TEFRDRLHEEYGYIVRTTSFYLDQYDTVKTYSLRISTHLFHDEDDVKGLVKAMKQLHREMR

Sequences:

>Translated_461_residues
MNHKPEANLANAGRRGFLKTTTGVALAAVGTSLLPATAHASSDNHIEPFHGHGSGQGFWNKVRNEFILNKRTTYMNIGTA
GTMPKRVLKDYNRTNEQISINPWNSNIPTLDYAKQIAPSFGADEHELVLCRNTTDGLCTIINGLQFEYGDIILTTNHEHH
GVTTPLKHVAQRYGAEVIELALPVYTPNNEVSSDDFVQVFNDAVSQYGNRVRLMVFSHVTFTTGTTLPAKRICQEVAIPN
GIITLVDGAHTPGMFNLDFHDIDCDFYSGAGHKWQCGPGATGFLYVRDNANRLFEFWSDRETPLWFINTSSSAAEVQTKL
QSVGQDNYPAKRALADACALWDEIGRDAIEERILDLSALCKKQLAKKFPEAMIFAPNIRELSSGLTTFNPFDDLNDGELL
TEFRDRLHEEYGYIVRTTSFYLDQYDTVKTYSLRISTHLFHDEDDVKGLVKAMKQLHREMR
>Mature_461_residues
MNHKPEANLANAGRRGFLKTTTGVALAAVGTSLLPATAHASSDNHIEPFHGHGSGQGFWNKVRNEFILNKRTTYMNIGTA
GTMPKRVLKDYNRTNEQISINPWNSNIPTLDYAKQIAPSFGADEHELVLCRNTTDGLCTIINGLQFEYGDIILTTNHEHH
GVTTPLKHVAQRYGAEVIELALPVYTPNNEVSSDDFVQVFNDAVSQYGNRVRLMVFSHVTFTTGTTLPAKRICQEVAIPN
GIITLVDGAHTPGMFNLDFHDIDCDFYSGAGHKWQCGPGATGFLYVRDNANRLFEFWSDRETPLWFINTSSSAAEVQTKL
QSVGQDNYPAKRALADACALWDEIGRDAIEERILDLSALCKKQLAKKFPEAMIFAPNIRELSSGLTTFNPFDDLNDGELL
TEFRDRLHEEYGYIVRTTSFYLDQYDTVKTYSLRISTHLFHDEDDVKGLVKAMKQLHREMR

Specific function: Catalyzes the reversible isomerization between isopenicillin N and penicillin N [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI1787970, Length=342, Percent_Identity=25.1461988304094, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =5.1.1.17 [H]

Molecular weight: Translated: 51730; Mature: 51730

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHKPEANLANAGRRGFLKTTTGVALAAVGTSLLPATAHASSDNHIEPFHGHGSGQGFWN
CCCCCCCCHHCCCCCCCEEHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
KVRNEFILNKRTTYMNIGTAGTMPKRVLKDYNRTNEQISINPWNSNIPTLDYAKQIAPSF
HHHHHHEECCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCC
GADEHELVLCRNTTDGLCTIINGLQFEYGDIILTTNHEHHGVTTPLKHVAQRYGAEVIEL
CCCCCEEEEEECCCCCHHHHHHCCEEECCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEH
ALPVYTPNNEVSSDDFVQVFNDAVSQYGNRVRLMVFSHVTFTTGTTLPAKRICQEVAIPN
EEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCHHHHHHHHCCCC
GIITLVDGAHTPGMFNLDFHDIDCDFYSGAGHKWQCGPGATGFLYVRDNANRLFEFWSDR
CEEEEEECCCCCCEEECCEEECCCEEECCCCCEEECCCCCCEEEEEECCHHHHHHHHCCC
ETPLWFINTSSSAAEVQTKLQSVGQDNYPAKRALADACALWDEIGRDAIEERILDLSALC
CCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
KKQLAKKFPEAMIFAPNIRELSSGLTTFNPFDDLNDGELLTEFRDRLHEEYGYIVRTTSF
HHHHHHHCCCCEEECCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEH
YLDQYDTVKTYSLRISTHLFHDEDDVKGLVKAMKQLHREMR
HHCCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNHKPEANLANAGRRGFLKTTTGVALAAVGTSLLPATAHASSDNHIEPFHGHGSGQGFWN
CCCCCCCCHHCCCCCCCEEHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
KVRNEFILNKRTTYMNIGTAGTMPKRVLKDYNRTNEQISINPWNSNIPTLDYAKQIAPSF
HHHHHHEECCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCC
GADEHELVLCRNTTDGLCTIINGLQFEYGDIILTTNHEHHGVTTPLKHVAQRYGAEVIEL
CCCCCEEEEEECCCCCHHHHHHCCEEECCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEH
ALPVYTPNNEVSSDDFVQVFNDAVSQYGNRVRLMVFSHVTFTTGTTLPAKRICQEVAIPN
EEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCHHHHHHHHCCCC
GIITLVDGAHTPGMFNLDFHDIDCDFYSGAGHKWQCGPGATGFLYVRDNANRLFEFWSDR
CEEEEEECCCCCCEEECCEEECCCEEECCCCCEEECCCCCCEEEEEECCHHHHHHHHCCC
ETPLWFINTSSSAAEVQTKLQSVGQDNYPAKRALADACALWDEIGRDAIEERILDLSALC
CCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
KKQLAKKFPEAMIFAPNIRELSSGLTTFNPFDDLNDGELLTEFRDRLHEEYGYIVRTTSF
HHHHHHHCCCCEEECCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEH
YLDQYDTVKTYSLRISTHLFHDEDDVKGLVKAMKQLHREMR
HHCCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1694525; 2804141 [H]